Rmu_sc0006646.1_g000001
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006646.1
OG0006868
Physical Location & Seq
Forward (+)
1 .. 4749
4749 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1138 bp
ctttccattccctccttcaaaaactactacgcgtcctttccatgtcaccaaaaccctaattactaattaccatctctctcgccgacgcctaccttcttctaattcatgctcgcttcgccctcccaagtccaatccactctacgacgtcgtccgatccacctccgcctccatggacggcgacaaggacacacgcgatccgggactcaccgacttccccaccgagccgagctgggacagtccttacttcttcagcaacgatcgtgacaccaccgtactaagcgaattcgggtggaatttcagccccgacgaccagatcggcgccgacgacaggtccgatttggcgggaagcagcactactactactctcgcggaacagtttccagaaagaagcagtaatactaactccgacccggcccccgtggtcggatctgtttcgtcaagctctacagaggatccgccggagaggtctactggctccggctccggcggcacaccacctcccgagacaccgagtagcactacgaaggtgaataaaaagaaggggcagaagcgaatccggcagccaagatttgcgtttatgactaagagtgaagttgatcatctcgaggatggctaccgttggcggaaatatggacagaaggctgttaagaatagcccatatccaaggagctactatcggtgcacaaatagcaaatgcactgtgaagaaaagggttgaacgttcctctgaagatccaacgacggtaattacgacgtatgaagggcaacactgtcatcataccgttgcatttccccgcggcggcgggggagtgaatattattagtcatgatcaatctggctttgccgcaggaagccagttaatgcagcttcctccagttgcgcagttcatgcattatccaacttcaattcaacccagatcacaaattagtccaactcataatttgacagtaggagggtcgcagtcgcaccgattagtccgggctggcggtactcattaccatcatgatcaagcagctgggggatcccagtgcattattaatccaaatcatgagccactcccagtagtaccagttcctacagatgaagggcttcttggggacattgtacctcctggaatgcgtaatccgttcgatggataa

Protein Analysis

378

Amino Acids

41.42

Weight (kDa)

8.52

Isoelectric Point (pI)

69.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 202 - 258 1.3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 148
Acc16I TGCGCA 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 318
AccI GTMKAC 1 cut(s) 468
AccII CGCG 4 cut(s) 32, 193, 369, 796
AclI AACGTT 1 cut(s) 719
AclWI GGATC 8 cut(s) 148, 189, 434, 447, 460, 726, 1015, 1028
AcsI RAATTY 2 cut(s) 282, 293
AcuI CTGAAG 2 cut(s) 233, 748
AcyI GRCGYC 3 cut(s) 86, 145, 319
AfaI GTAC 4 cut(s) 274, 989, 1066, 1105
AfiI CCNNNNNNNGG 4 cut(s) 328, 423, 798, 1131
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 4 cut(s) 19, 717, 904, 909
AhdI GACNNNNNGTC 1 cut(s) 329
AjnI CCWGG 1 cut(s) 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 1075, 1107
AluBI AGCT 5 cut(s) 229, 442, 670, 866, 1014
AluI AGCT 5 cut(s) 229, 442, 670, 866, 1014
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 1 cut(s) 498
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 8 cut(s) 148, 189, 434, 447, 460, 726, 1015, 1028
Ama87I CYCGRG 2 cut(s) 501, 603
AoxI GGCC 1 cut(s) 412
ApaLI GTGCAC 1 cut(s) 680
ApeKI GCWGC 4 cut(s) 349, 560, 863, 1011
ApoI RAATTY 2 cut(s) 282, 293
AseI ATTAAT 1 cut(s) 1036
Asp700I GAANNNNTTC 2 cut(s) 376, 1087
AspLEI GCGC 2 cut(s) 321, 881
AspS9I GGNCC 2 cut(s) 331, 413
AsuC2I CCSGG 3 cut(s) 199, 411, 978
AsuHPI GGTGA 3 cut(s) 38, 197, 539
AvaI CYCGRG 2 cut(s) 501, 603
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 684
BamHI GGATCC 2 cut(s) 452, 1020
BanI GGYRCC 1 cut(s) 318
BarI GAAGNNNNNNTAC 4 cut(s) 338, 370, 380, 412
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 4 cut(s) 361, 572, 875, 1023
BccI CCATC 4 cut(s) 79, 603, 1006, 1125
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 1111
BclI TGATCA 3 cut(s) 596, 827, 1004
BcnI CCSGG 3 cut(s) 199, 411, 978
BcoDI GTCTC 1 cut(s) 498
BfmI CTRYAG 2 cut(s) 445, 1075
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 8 cut(s) 350, 488, 561, 797, 800, 844, 864, 1012
BlsI GCNGC 8 cut(s) 351, 489, 562, 798, 801, 845, 865, 1013
Bme1390I CCNGG 4 cut(s) 199, 411, 978, 1111
Bme18I GGWCC 1 cut(s) 331
BmeRI GACNNNNNGTC 1 cut(s) 329
BmeT110I CYCGRG 2 cut(s) 501, 603
BmgT120I GGNCC 2 cut(s) 331, 413
BmiI GGNNCC 6 cut(s) 320, 415, 454, 476, 482, 1022
BmrFI CCNGG 4 cut(s) 199, 411, 978, 1111
BmrI ACTGGG 2 cut(s) 1019, 1053
BmuI ACTGGG 2 cut(s) 1019, 1053
BpmI CTGGAG 1 cut(s) 856
BpuMI CCSGG 3 cut(s) 199, 411, 978
BsaHI GRCGYC 3 cut(s) 86, 145, 319
BsaJI CCNNGG 4 cut(s) 169, 418, 663, 794
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 4 cut(s) 328, 423, 798, 1131
Bse1I ACTGG 6 cut(s) 476, 854, 873, 1025, 1059, 1068
BseBI CCWGG 1 cut(s) 1111
BseDI CCNNGG 4 cut(s) 169, 418, 663, 794
BseGI GGATG 1 cut(s) 614
BseLI CCNNNNNNNGG 4 cut(s) 328, 423, 798, 1131
BseNI ACTGG 6 cut(s) 476, 854, 873, 1025, 1059, 1068
BseSI GKGCMC 1 cut(s) 684
BseXI GCAGC 4 cut(s) 361, 572, 875, 1023
BseYI CCCAGC 2 cut(s) 229, 1014
Bsh1236I CGCG 4 cut(s) 32, 193, 369, 796
Bsh1285I CGRYCG 1 cut(s) 260
BshFI GGCC 1 cut(s) 414
BshNI GGYRCC 1 cut(s) 318
BsiEI CGRYCG 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 684
BsiHKCI CYCGRG 2 cut(s) 501, 603
BsiSI CCGG 7 cut(s) 198, 411, 459, 478, 484, 557, 977
BslFI GGGAC 3 cut(s) 214, 246, 1110
BslI CCNNNNNNNGG 4 cut(s) 328, 423, 798, 1131
BsmAI GTCTC 1 cut(s) 498
BsmFI GGGAC 3 cut(s) 214, 246, 1110
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 1 cut(s) 414
BsoBI CYCGRG 2 cut(s) 501, 603
Bsp1286I GDGCHC 1 cut(s) 684
Bsp19I CCATGG 1 cut(s) 169
BspANI GGCC 1 cut(s) 414
BspFNI CGCG 4 cut(s) 32, 193, 369, 796
BspHI TCATGA 3 cut(s) 824, 1001, 1046
BspLI GGNNCC 6 cut(s) 320, 415, 454, 476, 482, 1022
BspPI GGATC 8 cut(s) 148, 189, 434, 447, 460, 726, 1015, 1028
BspT107I GGYRCC 1 cut(s) 318
BsrI ACTGG 6 cut(s) 476, 854, 873, 1025, 1059, 1068
BssECI CCNNGG 4 cut(s) 169, 418, 663, 794
BssNI GRCGYC 3 cut(s) 86, 145, 319
BssT1I CCWWGG 2 cut(s) 169, 663
Bst2UI CCWGG 1 cut(s) 1111
Bst4CI ACNGT 9 cut(s) 237, 272, 376, 618, 701, 743, 771, 782, 947
BstACI GRCGYC 3 cut(s) 86, 145, 319
BstAPI GCANNNNNTGC 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 111, 983
BstDEI CTNAG 2 cut(s) 276, 583
BstDSI CCRYGG 3 cut(s) 169, 418, 794
BstF5I GGATG 1 cut(s) 614
BstFNI CGCG 4 cut(s) 32, 193, 369, 796
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 321, 881
BstMAI GTCTC 1 cut(s) 498
BstMCI CGRYCG 1 cut(s) 260
BstMWI GCNNNNNNNGC 5 cut(s) 115, 487, 557, 688, 887
BstNI CCWGG 1 cut(s) 1111
BstSCI CCNGG 4 cut(s) 197, 409, 976, 1109
BstSFI CTRYAG 2 cut(s) 445, 1075
BstSLI GKGCMC 1 cut(s) 684
BstUI CGCG 4 cut(s) 32, 193, 369, 796
BstV1I GCAGC 4 cut(s) 361, 572, 875, 1023
BstX2I RGATCY 4 cut(s) 426, 452, 731, 1020
BstYI RGATCY 4 cut(s) 426, 452, 731, 1020
BsuRI GGCC 1 cut(s) 414
BtgI CCRYGG 3 cut(s) 169, 418, 794
BtsCI GGATG 1 cut(s) 614
BtsIMutI CAGTG 3 cut(s) 697, 767, 1032
Cac8I GCNNGC 2 cut(s) 111, 983
CciI TCATGA 3 cut(s) 824, 1001, 1046
CfoI GCGC 2 cut(s) 321, 881
Cfr13I GGNCC 2 cut(s) 331, 413
Cfr42I CCGCGG 1 cut(s) 797
CseI GACGC 2 cut(s) 21, 94
Csp6I GTAC 4 cut(s) 273, 988, 1065, 1104
CviAII CATG 7 cut(s) 42, 106, 170, 825, 887, 1002, 1047
CviQI GTAC 4 cut(s) 273, 988, 1065, 1104
DdeI CTNAG 2 cut(s) 276, 583
DinI GGCGCC 1 cut(s) 320
DriI GACNNNNNGTC 1 cut(s) 329
Eam1105I GACNNNNNGTC 1 cut(s) 329
EciI GGCGGA 3 cut(s) 152, 445, 638
Eco130I CCWWGG 2 cut(s) 169, 663
Eco47I GGWCC 1 cut(s) 331
Eco57I CTGAAG 2 cut(s) 233, 748
Eco88I CYCGRG 2 cut(s) 501, 603
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 1109
EcoT14I CCWWGG 2 cut(s) 169, 663
EcoT22I ATGCAT 1 cut(s) 892
EgeI GGCGCC 1 cut(s) 320
EheI GGCGCC 1 cut(s) 320
ErhI CCWWGG 2 cut(s) 169, 663
FaeI CATG 7 cut(s) 45, 109, 173, 828, 890, 1005, 1050
FalI AAGNNNNNCTT 2 cut(s) 1075, 1107
FaqI GGGAC 3 cut(s) 214, 246, 1110
FatI CATG 7 cut(s) 41, 105, 169, 824, 886, 1001, 1046
FauI CCCGC 3 cut(s) 335, 795, 801
FbaI TGATCA 3 cut(s) 596, 827, 1004
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 8 cut(s) 350, 488, 561, 797, 800, 844, 864, 1012
FokI GGATG 1 cut(s) 621
Fsp4HI GCNGC 8 cut(s) 350, 488, 561, 797, 800, 844, 864, 1012
FspI TGCGCA 1 cut(s) 880
GlaI GCGC 2 cut(s) 320, 880
GluI GCNGC 8 cut(s) 350, 488, 561, 797, 800, 844, 864, 1012
GsaI CCCAGC 2 cut(s) 233, 1018
GsuI CTGGAG 1 cut(s) 856
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 1 cut(s) 414
HapII CCGG 7 cut(s) 198, 411, 459, 478, 484, 557, 977
HgaI GACGC 2 cut(s) 21, 94
HhaI GCGC 2 cut(s) 321, 881
Hin1I GRCGYC 3 cut(s) 86, 145, 319
Hin1II CATG 7 cut(s) 45, 109, 173, 828, 890, 1005, 1050
Hin6I GCGC 2 cut(s) 319, 879
HinP1I GCGC 2 cut(s) 319, 879
HinfI GANTC 2 cut(s) 202, 553
HpaII CCGG 7 cut(s) 198, 411, 459, 478, 484, 557, 977
HphI GGTGA 3 cut(s) 38, 197, 539
Hpy166II GTNNAC 2 cut(s) 469, 682
Hpy188I TCNGA 5 cut(s) 153, 335, 407, 426, 728
Hpy188III TCNNGA 7 cut(s) 261, 381, 501, 603, 825, 1002, 1047
Hpy8I GTNNAC 2 cut(s) 469, 682
Hpy99I CGWCG 7 cut(s) 88, 147, 150, 309, 327, 743, 755
HpyAV CCTTC 7 cut(s) 25, 103, 518, 533, 632, 753, 1077
HpyCH4III ACNGT 9 cut(s) 237, 272, 376, 618, 701, 743, 771, 782, 947
HpyCH4IV ACGT 3 cut(s) 145, 719, 753
HpyCH4V TGCA 6 cut(s) 682, 697, 786, 863, 890, 1030
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 487, 557, 688, 887
HpyF3I CTNAG 2 cut(s) 276, 583
HpySE526I ACGT 3 cut(s) 145, 719, 753
Hsp92I GRCGYC 3 cut(s) 86, 145, 319
Hsp92II CATG 7 cut(s) 45, 109, 173, 828, 890, 1005, 1050
HspAI GCGC 2 cut(s) 319, 879
KasI GGCGCC 1 cut(s) 318
Ksp22I TGATCA 3 cut(s) 596, 827, 1004
KspI CCGCGG 1 cut(s) 797
LmnI GCTCC 3 cut(s) 480, 486, 667
Lsp1109I GCAGC 4 cut(s) 361, 572, 875, 1023
MaeII ACGT 3 cut(s) 145, 719, 753
MaeIII GTNAC 2 cut(s) 44, 261
MboII GAAGA 4 cut(s) 88, 239, 716, 741
MflI RGATCY 4 cut(s) 426, 452, 731, 1020
MhlI GDGCHC 1 cut(s) 684
MluCI AATT 9 cut(s) 58, 65, 101, 282, 293, 745, 904, 922, 938
MluI ACGCGT 1 cut(s) 30
Mly113I GGCGCC 1 cut(s) 319
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 5 cut(s) 404, 430, 759, 921, 954
Mph1103I ATGCAT 1 cut(s) 892
MroXI GAANNNNTTC 2 cut(s) 376, 1087
MseI TTAA 3 cut(s) 646, 858, 1036
MspA1I CMGCKG 2 cut(s) 796, 1014
MspI CCGG 7 cut(s) 198, 411, 459, 478, 484, 557, 977
MspR9I CCNGG 4 cut(s) 199, 411, 978, 1111
Mva1269I GAATGC 1 cut(s) 1120
MvaI CCWGG 1 cut(s) 1111
MvnI CGCG 4 cut(s) 32, 193, 369, 796
MwoI GCNNNNNNNGC 5 cut(s) 115, 487, 557, 688, 887
NarI GGCGCC 1 cut(s) 319
NciI CCSGG 3 cut(s) 199, 411, 978
NcoI CCATGG 1 cut(s) 169
NlaIII CATG 7 cut(s) 45, 109, 173, 828, 890, 1005, 1050
NlaIV GGNNCC 6 cut(s) 320, 415, 454, 476, 482, 1022
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 44, 261
NsbI TGCGCA 1 cut(s) 880
NsiI ATGCAT 1 cut(s) 892
PaeR7I CTCGAG 1 cut(s) 603
PagI TCATGA 3 cut(s) 824, 1001, 1046
PcsI WCGNNNNNNNCGW 3 cut(s) 322, 331, 747
PctI GAATGC 1 cut(s) 1120
PdmI GAANNNNTTC 2 cut(s) 376, 1087
PfeI GAWTC 1 cut(s) 553
PflFI GACNNNGTC 1 cut(s) 147
PfoI TCCNGGA 2 cut(s) 197, 1109
PkrI GCNGC 8 cut(s) 351, 489, 562, 798, 801, 845, 865, 1013
Ple19I CGATCG 1 cut(s) 260
PleI GAGTC 1 cut(s) 196
PluTI GGCGCC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 196
PshBI ATTAAT 1 cut(s) 1036
Psp1406I AACGTT 1 cut(s) 719
Psp6I CCWGG 1 cut(s) 1109
PspFI CCCAGC 2 cut(s) 229, 1014
PspGI CCWGG 1 cut(s) 1109
PspN4I GGNNCC 6 cut(s) 320, 415, 454, 476, 482, 1022
PspPI GGNCC 2 cut(s) 331, 413
PsrI GAACNNNNNNTAC 2 cut(s) 1054, 1086
PsuI RGATCY 4 cut(s) 426, 452, 731, 1020
PsyI GACNNNGTC 1 cut(s) 147
PvuI CGATCG 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 1014
RsaI GTAC 4 cut(s) 274, 989, 1066, 1105
RsaNI GTAC 4 cut(s) 273, 988, 1065, 1104
SacII CCGCGG 1 cut(s) 797
SaqAI TTAA 3 cut(s) 646, 858, 1036
SatI GCNGC 8 cut(s) 350, 488, 561, 797, 800, 844, 864, 1012
Sau96I GGNCC 2 cut(s) 331, 413
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 4 cut(s) 199, 411, 978, 1111
SduI GDGCHC 1 cut(s) 684
SfcI CTRYAG 2 cut(s) 445, 1075
SfoI GGCGCC 1 cut(s) 320
Sfr274I CTCGAG 1 cut(s) 603
Sfr303I CCGCGG 1 cut(s) 797
SgrBI CCGCGG 1 cut(s) 797
SinI GGWCC 1 cut(s) 331
SlaI CTCGAG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
Sse9I AATT 9 cut(s) 58, 65, 101, 282, 293, 745, 904, 922, 938
SspDI GGCGCC 1 cut(s) 318
SspI AATATT 1 cut(s) 815
StyD4I CCNGG 4 cut(s) 197, 409, 976, 1109
StyI CCWWGG 2 cut(s) 169, 663
TaaI ACNGT 9 cut(s) 237, 272, 376, 618, 701, 743, 771, 782, 947
TaiI ACGT 3 cut(s) 148, 722, 756
TaqI TCGA 2 cut(s) 604, 1129
TasI AATT 9 cut(s) 58, 65, 101, 282, 293, 745, 904, 922, 938
TauI GCSGC 4 cut(s) 490, 799, 802, 846
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 3 cut(s) 646, 858, 1036
Tru9I TTAA 3 cut(s) 646, 858, 1036
TscAI CASTG 3 cut(s) 704, 774, 1032
TseFI GTSAC 2 cut(s) 44, 261
TseI GCWGC 4 cut(s) 349, 560, 863, 1011
Tsp45I GTSAC 2 cut(s) 44, 261
TspDTI ATGAA 4 cut(s) 94, 772, 875, 1096
TspGWI ACGGA 1 cut(s) 1114
TspRI CASTG 3 cut(s) 704, 774, 1032
Tth111I GACNNNGTC 1 cut(s) 147
VneI GTGCAC 1 cut(s) 680
VpaK11BI GGWCC 1 cut(s) 331
VspI ATTAAT 1 cut(s) 1036
XapI RAATTY 2 cut(s) 282, 293
XhoI CTCGAG 1 cut(s) 603
XmiI GTMKAC 1 cut(s) 468
XmnI GAANNNNTTC 2 cut(s) 376, 1087
ZraI GACGTC 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.