Rmu_sc0006736.1_g000032

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006736.1
OG0016471
Physical Location & Seq
Reverse (-)
99493 .. 102267
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006736.1_g000032.1.cds

Sequence Viewer

Length: 1191 bp
atggaagcaactccaactgttgaggctgagatcgaagtggctagagtgcctattgtgttgggggaattggacattgtcaagccaactccagctacagaagtgcaggtgaaggcttctcttttgatccttacgcaaactgtagactccagtgatagcgtacatccaggcttccaaaggttggtcattaagactaaagcccactcttcaagctccgcatcaactccagtgttgtcagcagtagctcgttctattatgaatgccaacggcatcgcaagtgatgacacgcaacgagctttatgtaaaatggttcgtgctcacaaatcgggatattgtccctgtaattatctattctactgctcaacaggacaacagatcacagtttatgttgctcttgatgatttcaaaatatgtggctgcagcaccttgatttccaaagtatggttagtaacttctggtctctggcaattctgctggatgtcgaatgtttgtgctttcctcaaaacatcgagcactgaaatttatgctcaaagattggaatgccaataccttaaggaatgtgcatcaactggtaagttcctccaaagctgcactagatttggttcacatgaaatttataatttgagtccttcagaagatcgtttacagagagtgacgctagctcatgtgcagtttcagcaagctttggcaatgcaagacactctttttagagacaaagctagaatcagatggtttcgtgagggggatcggaatacctccttcctacataacatgacggctttcaccaaatatgacagtcttctagacactagcattgtagaaagagtaatcccaaggggtgtgagacaaggtgatcccttgtctccactcttattcttcattgcgaaggaggttctttgtagggggttaagtaatccggtggacaagggaaaaattgattggttggcttgtccaggggagctcccgtggtatacgtgccttgatgagtttttagatgaatatgcacttaattctatccaagtaattaataaagcaaagtcttatgtgtttattggtaagcatgcgcgggctagagaagttatcataaaacgccttctttggtttaaagagggtgcaatgcccttcaattatcttggagtgccggtttttcaaggtagacctaaagcagcttattttacatctattgctgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

396

Amino Acids

44.45

Weight (kDa)

8.57

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AarI CACCTGC 1 cut(s) 94
Acc36I ACCTGC 1 cut(s) 94
AccB7I CCANNNNNTGG 2 cut(s) 178, 438
AccI GTMKAC 3 cut(s) 141, 968, 1153
AccII CGCG 1 cut(s) 1063
AciI CCGC 2 cut(s) 213, 1063
AclWI GGATC 3 cut(s) 118, 750, 845
AcsI RAATTY 2 cut(s) 516, 609
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 178, 438
AflII CTTAAG 1 cut(s) 548
AgsI TTSAA 4 cut(s) 207, 403, 1123, 1148
AjnI CCWGG 2 cut(s) 163, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 919, 951
Alw21I GWGCWC 3 cut(s) 316, 512, 960
Alw26I GTCTC 4 cut(s) 461, 702, 835, 864
AlwI GGATC 3 cut(s) 118, 750, 845
ApeKI GCWGC 4 cut(s) 414, 417, 585, 1163
ApoI RAATTY 2 cut(s) 516, 609
AseI ATTAAT 1 cut(s) 1023
AspLEI GCGC 1 cut(s) 1063
AsuHPI GGTGA 3 cut(s) 118, 772, 860
AsuNHI GCTAGC 1 cut(s) 655
BanII GRGCYC 1 cut(s) 960
BarI GAAGNNNNNNTAC 2 cut(s) 624, 656
BbsI GAAGAC 1 cut(s) 788
Bbv12I GWGCWC 3 cut(s) 316, 512, 960
BbvI GCAGC 4 cut(s) 401, 429, 572, 1175
BccI CCATC 1 cut(s) 720
BceAI ACGGC 2 cut(s) 280, 789
BciT130I CCWGG 2 cut(s) 165, 951
BcoDI GTCTC 4 cut(s) 461, 702, 835, 864
BfaI CTAG 7 cut(s) 42, 591, 656, 717, 800, 807, 1068
BfmI CTRYAG 3 cut(s) 93, 138, 415
BfrI CTTAAG 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 4 cut(s) 415, 418, 586, 1164
BlsI GCNGC 4 cut(s) 416, 419, 587, 1165
Bme1390I CCNGG 2 cut(s) 165, 951
BmrFI CCNGG 2 cut(s) 165, 951
BmsI GCATC 3 cut(s) 224, 276, 569
BmtI GCTAGC 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 788
BpmI CTGGAG 3 cut(s) 72, 130, 207
BsaAI YACGTR 1 cut(s) 972
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 830, 950, 962
BsaWI WCCGGW 1 cut(s) 913
BsaXI ACNNNNNCTCC 4 cut(s) 947, 977, 1125, 1155
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 438
Bse118I RCCGGY 1 cut(s) 1138
Bse1I ACTGG 3 cut(s) 147, 224, 571
Bse3DI GCAATG 3 cut(s) 693, 876, 1119
BseBI CCWGG 2 cut(s) 165, 951
BseDI CCNNGG 3 cut(s) 830, 950, 962
BseGI GGATG 2 cut(s) 160, 480
BseLI CCNNNNNNNGG 2 cut(s) 178, 438
BseMI GCAATG 3 cut(s) 693, 876, 1119
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 3 cut(s) 147, 224, 571
BseXI GCAGC 4 cut(s) 401, 429, 572, 1175
BsgI GTGCAG 3 cut(s) 122, 571, 686
Bsh1236I CGCG 1 cut(s) 1063
BsiHKAI GWGCWC 3 cut(s) 316, 512, 960
BsiSI CCGG 2 cut(s) 914, 1139
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 178, 438
BsmAI GTCTC 4 cut(s) 461, 702, 835, 864
BsmFI GGGAC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 262, 542
Bso31I GGTCTC 1 cut(s) 461
Bsp1286I GDGCHC 3 cut(s) 316, 512, 960
Bsp143I GATC 6 cut(s) 30, 123, 372, 634, 742, 850
BspACI CCGC 2 cut(s) 213, 1063
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 1063
BspMAI CTGCAG 1 cut(s) 419
BspMI ACCTGC 1 cut(s) 94
BspOI GCTAGC 1 cut(s) 659
BspPI GGATC 3 cut(s) 118, 750, 845
BspTI CTTAAG 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 3 cut(s) 693, 876, 1119
BsrFI RCCGGY 1 cut(s) 1138
BsrI ACTGG 3 cut(s) 147, 224, 571
BssAI RCCGGY 1 cut(s) 1138
BssECI CCNNGG 3 cut(s) 830, 950, 962
BssMI GATC 6 cut(s) 30, 123, 372, 634, 742, 850
BssNAI GTATAC 1 cut(s) 969
BssT1I CCWWGG 1 cut(s) 830
Bst1107I GTATAC 1 cut(s) 969
Bst2UI CCWGG 2 cut(s) 165, 951
Bst4CI ACNGT 4 cut(s) 19, 139, 379, 794
Bst6I CTCTTC 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 972
BstC8I GCNNGC 4 cut(s) 657, 678, 1059, 1065
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 962
BstF5I GGATG 2 cut(s) 160, 480
BstFNI CGCG 1 cut(s) 1063
BstHHI GCGC 1 cut(s) 1063
BstKTI GATC 6 cut(s) 33, 126, 375, 637, 745, 853
BstMAI GTCTC 4 cut(s) 461, 702, 835, 864
BstMBI GATC 6 cut(s) 30, 123, 372, 634, 742, 850
BstMWI GCNNNNNNNGC 1 cut(s) 673
BstNI CCWGG 2 cut(s) 165, 951
BstNSI RCATGY 1 cut(s) 1061
BstSCI CCNGG 2 cut(s) 163, 949
BstSFI CTRYAG 3 cut(s) 93, 138, 415
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 4 cut(s) 401, 429, 572, 1175
BstV2I GAAGAC 1 cut(s) 788
BstZ17I GTATAC 1 cut(s) 969
BtgI CCRYGG 1 cut(s) 962
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 2 cut(s) 160, 480
BtsIMutI CAGTG 3 cut(s) 154, 231, 510
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 4 cut(s) 657, 678, 1059, 1065
CfoI GCGC 1 cut(s) 1063
Cfr10I RCCGGY 1 cut(s) 1138
CseI GACGC 1 cut(s) 661
Csp6I GTAC 1 cut(s) 158
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 4 cut(s) 605, 662, 769, 1058
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 1 cut(s) 27
DpnI GATC 6 cut(s) 32, 125, 374, 636, 744, 852
DpnII GATC 6 cut(s) 30, 123, 372, 634, 742, 850
DraI TTTAAA 1 cut(s) 1102
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 958
Eco130I CCWWGG 1 cut(s) 830
Eco24I GRGCYC 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 958
Eco57I CTGAAG 1 cut(s) 612
EcoICRI GAGCTC 1 cut(s) 958
EcoRII CCWGG 2 cut(s) 163, 949
EcoT14I CCWWGG 1 cut(s) 830
EcoT38I GRGCYC 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 830
FaeI CATG 4 cut(s) 608, 665, 772, 1061
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 1 cut(s) 318
FatI CATG 4 cut(s) 604, 661, 768, 1057
FauI CCCGC 1 cut(s) 1056
FblI GTMKAC 3 cut(s) 141, 968, 1153
Fnu4HI GCNGC 4 cut(s) 415, 418, 586, 1164
FokI GGATG 2 cut(s) 147, 487
FriOI GRGCYC 1 cut(s) 960
Fsp4HI GCNGC 4 cut(s) 415, 418, 586, 1164
FspBI CTAG 7 cut(s) 42, 591, 656, 717, 800, 807, 1068
GlaI GCGC 1 cut(s) 1062
GluI GCNGC 4 cut(s) 415, 418, 586, 1164
GsuI CTGGAG 3 cut(s) 72, 130, 207
HapII CCGG 2 cut(s) 914, 1139
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 1063
Hin1II CATG 4 cut(s) 608, 665, 772, 1061
Hin6I GCGC 1 cut(s) 1061
HinP1I GCGC 1 cut(s) 1061
HindIII AAGCTT 1 cut(s) 678
HinfI GANTC 3 cut(s) 143, 622, 720
HpaII CCGG 2 cut(s) 914, 1139
HphI GGTGA 3 cut(s) 118, 772, 860
Hpy166II GTNNAC 6 cut(s) 142, 602, 641, 919, 969, 1154
Hpy188I TCNGA 3 cut(s) 631, 725, 747
Hpy188III TCNNGA 4 cut(s) 324, 392, 734, 800
Hpy8I GTNNAC 6 cut(s) 142, 602, 641, 919, 969, 1154
HpyAV CCTTC 6 cut(s) 103, 636, 766, 877, 1100, 1129
HpyCH4III ACNGT 4 cut(s) 19, 139, 379, 794
HpyCH4IV ACGT 1 cut(s) 971
HpyCH4V TGCA 8 cut(s) 103, 417, 560, 588, 667, 691, 1001, 1112
HpyF10VI GCNNNNNNNGC 1 cut(s) 673
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 971
Hsp92II CATG 4 cut(s) 608, 665, 772, 1061
HspAI GCGC 1 cut(s) 1061
Kzo9I GATC 6 cut(s) 30, 123, 372, 634, 742, 850
LmnI GCTCC 3 cut(s) 215, 955, 963
Lsp1109I GCAGC 4 cut(s) 401, 429, 572, 1175
LweI GCATC 3 cut(s) 224, 276, 569
MaeI CTAG 7 cut(s) 42, 591, 656, 717, 800, 807, 1068
MaeII ACGT 1 cut(s) 971
MaeIII GTNAC 2 cut(s) 445, 649
MalI GATC 6 cut(s) 32, 125, 374, 636, 744, 852
MboI GATC 6 cut(s) 30, 123, 372, 634, 742, 850
MboII GAAGA 4 cut(s) 195, 644, 788, 865
MhlI GDGCHC 3 cut(s) 316, 512, 960
MlyI GAGTC 2 cut(s) 137, 631
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 7 cut(s) 16, 506, 587, 730, 763, 880, 1099
MseI TTAA 6 cut(s) 186, 549, 905, 1005, 1023, 1101
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 2 cut(s) 914, 1139
MspR9I CCNGG 2 cut(s) 165, 951
Mva1269I GAATGC 2 cut(s) 262, 542
MvaI CCWGG 2 cut(s) 165, 951
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 1 cut(s) 673
NdeII GATC 6 cut(s) 30, 123, 372, 634, 742, 850
NheI GCTAGC 1 cut(s) 655
NlaIII CATG 4 cut(s) 608, 665, 772, 1061
NmuCI GTSAC 1 cut(s) 649
NspI RCATGY 1 cut(s) 1061
PaeI GCATGC 1 cut(s) 1061
PaqCI CACCTGC 1 cut(s) 94
PctI GAATGC 2 cut(s) 262, 542
PfeI GAWTC 1 cut(s) 720
PflFI GACNNNGTC 1 cut(s) 74
PflMI CCANNNNNTGG 2 cut(s) 178, 438
PkrI GCNGC 4 cut(s) 416, 419, 587, 1165
PleI GAGTC 2 cut(s) 137, 630
PpsI GAGTC 2 cut(s) 137, 630
Ppu21I YACGTR 1 cut(s) 972
PshBI ATTAAT 1 cut(s) 1023
PsiI TTATAA 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 960
Psp6I CCWGG 2 cut(s) 163, 949
PspGI CCWGG 2 cut(s) 163, 949
PstI CTGCAG 1 cut(s) 419
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
SacI GAGCTC 1 cut(s) 960
SaqAI TTAA 6 cut(s) 186, 549, 905, 1005, 1023, 1101
SatI GCNGC 4 cut(s) 415, 418, 586, 1164
Sau3AI GATC 6 cut(s) 30, 123, 372, 634, 742, 850
SchI GAGTC 2 cut(s) 137, 631
ScrFI CCNGG 2 cut(s) 165, 951
SduI GDGCHC 3 cut(s) 316, 512, 960
SfaNI GCATC 3 cut(s) 224, 276, 569
SfcI CTRYAG 3 cut(s) 93, 138, 415
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SphI GCATGC 1 cut(s) 1061
SsiI CCGC 2 cut(s) 213, 1063
SspMI CTAG 7 cut(s) 42, 591, 656, 717, 800, 807, 1068
SstI GAGCTC 1 cut(s) 960
StyD4I CCNGG 2 cut(s) 163, 949
StyI CCWWGG 1 cut(s) 830
TaaI ACNGT 4 cut(s) 19, 139, 379, 794
TaiI ACGT 1 cut(s) 974
TaqI TCGA 3 cut(s) 33, 479, 506
TfiI GAWTC 1 cut(s) 720
Tru1I TTAA 6 cut(s) 186, 549, 905, 1005, 1023, 1101
Tru9I TTAA 6 cut(s) 186, 549, 905, 1005, 1023, 1101
TscAI CASTG 3 cut(s) 154, 231, 517
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 4 cut(s) 414, 417, 585, 1163
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 4 cut(s) 269, 621, 865, 1008
TspRI CASTG 3 cut(s) 154, 231, 517
Tth111I GACNNNGTC 1 cut(s) 74
Van91I CCANNNNNTGG 2 cut(s) 178, 438
Vha464I CTTAAG 1 cut(s) 548
VspI ATTAAT 1 cut(s) 1023
XapI RAATTY 2 cut(s) 516, 609
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 1 cut(s) 1061
XmiI GTMKAC 3 cut(s) 141, 968, 1153
XspI CTAG 7 cut(s) 42, 591, 656, 717, 800, 807, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.