Rmu_sc0024469.1_g000006

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024469.1
OG0016471
Physical Location & Seq
Forward (+)
13581 .. 16368
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024469.1_g000006.1.cds

Sequence Viewer

Length: 1299 bp
atggaagcaactccaactgttgaggctgagatcgaagtggctagagtgcctattgtgttgggggaattggacattgtcaagccaactccagctacagaagtgcaggtgaaggcttctcttttgatccttacgcaaactgtagactccagtgatagcgtacatccaggcttccaaaggttggtcattaagactaaagcccactcttcaagctccgcatcaactccagtgttgtcagcagtagctcgttctattatgaatgccaacggcatcgcaagtgatgacacgcaacgagctttatgtaaaatggttcgtgctcacaaaccggatctcaccgatattcactctaactttgtggtgggtccatggatagtctttggcgattttaattgtgttatgggagctcatgaaaaaagaggtggggacccacctagtcctatctcttgcattgattttcagcagatgtgttatgcatgccatcttattgatgtgcatacaagaggtctatcttacacttggacaaaccggagaacatatgaaaggtttgatagggcactttgtaacatgggttggatggatgcatgtagtatttttgattgctgtactttaactaaagtggcttctgatcattgtcctttattattgagctgctctcgtctgccatttgtttctaaacctcagttcagatttcaaaatatgtggctgcagcaccttgatttccaaagtatggttagtaacttctggtcttctggcaattctgctggatgtcgaatgtttgtgctttcctcaaaacatcgagcactgaaatttatgctcaaagattggaatgccaataccttaaggaatgtgcatcaactgacaatcatggcaaaggaggtaacattgctcttaaatttgatatcagaaaagcctttgacactttggactgggattttctacttagggggtgtgagacaaggtgatcccttgtctccactcttattcttcattgcgaaggaggttctttgtagggggttaagtaatccggtggacaagggaaaaattgattggttggcttgtccaggggagctcccgtggtatacgtgccttgatgagtttttagatgaatatgcacttaattctatccaagtaattaataaagcaaagtcttatgtgtttattggtaagcatgcacagcctagagaagttatcatacaacgcctactttggtttaaagagggtgcaatgcccttcaattatcttggagtgccggtttttcaaggtagacctaaagcagcttattttacatctattgctgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

432

Amino Acids

48.43

Weight (kDa)

8.3

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
Acc36I ACCTGC 1 cut(s) 94
AccB7I CCANNNNNTGG 2 cut(s) 178, 724
AccI GTMKAC 3 cut(s) 141, 1076, 1261
AciI CCGC 1 cut(s) 213
AclWI GGATC 3 cut(s) 118, 333, 953
AcsI RAATTY 2 cut(s) 803, 889
AfaI GTAC 2 cut(s) 159, 601
AfiI CCNNNNNNNGG 2 cut(s) 178, 724
AflII CTTAAG 1 cut(s) 835
AgsI TTSAA 4 cut(s) 207, 689, 1231, 1256
AjnI CCWGG 2 cut(s) 163, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 1027, 1059
AluBI AGCT 8 cut(s) 92, 210, 242, 293, 401, 645, 1066, 1274
AluI AGCT 8 cut(s) 92, 210, 242, 293, 401, 645, 1066, 1274
Alw21I GWGCWC 4 cut(s) 316, 403, 799, 1068
Alw26I GTCTC 2 cut(s) 943, 972
AlwI GGATC 3 cut(s) 118, 333, 953
ApeKI GCWGC 4 cut(s) 645, 700, 703, 1271
ApoI RAATTY 2 cut(s) 803, 889
AseI ATTAAT 1 cut(s) 1131
AspS9I GGNCC 2 cut(s) 359, 421
AsuHPI GGTGA 3 cut(s) 118, 322, 968
AvaII GGWCC 2 cut(s) 359, 421
BaeGI GKGCMC 1 cut(s) 553
BanII GRGCYC 2 cut(s) 403, 1068
BarI GAAGNNNNNNTAC 2 cut(s) 1173, 1205
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 4 cut(s) 316, 403, 799, 1068
BbvI GCAGC 4 cut(s) 632, 687, 715, 1283
BccI CCATC 2 cut(s) 483, 565
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 2 cut(s) 165, 1059
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 943, 972
BfaI CTAG 3 cut(s) 42, 429, 1176
BfmI CTRYAG 3 cut(s) 93, 138, 701
BfrI CTTAAG 1 cut(s) 835
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 4 cut(s) 646, 701, 704, 1272
BlsI GCNGC 4 cut(s) 647, 702, 705, 1273
Bme1390I CCNGG 2 cut(s) 165, 1059
Bme18I GGWCC 2 cut(s) 359, 421
BmgT120I GGNCC 2 cut(s) 359, 421
BmiI GGNNCC 3 cut(s) 360, 422, 423
BmrFI CCNGG 2 cut(s) 165, 1059
BmrI ACTGGG 1 cut(s) 933
BmsI GCATC 4 cut(s) 224, 276, 565, 856
BmuI ACTGGG 1 cut(s) 933
BpiI GAAGAC 1 cut(s) 735
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpmI CTGGAG 3 cut(s) 72, 130, 207
BsaAI YACGTR 1 cut(s) 1080
BsaJI CCNNGG 3 cut(s) 362, 1058, 1070
BsaWI WCCGGW 3 cut(s) 322, 522, 1021
BsaXI ACNNNNNCTCC 4 cut(s) 1055, 1085, 1233, 1263
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 724
Bse118I RCCGGY 1 cut(s) 1246
Bse1I ACTGG 3 cut(s) 147, 224, 928
Bse3DI GCAATG 3 cut(s) 878, 984, 1227
BseBI CCWGG 2 cut(s) 165, 1059
BseDI CCNNGG 3 cut(s) 362, 1058, 1070
BseGI GGATG 4 cut(s) 160, 576, 580, 767
BseLI CCNNNNNNNGG 2 cut(s) 178, 724
BseMI GCAATG 3 cut(s) 878, 984, 1227
BseMII CTCAG 2 cut(s) 18, 689
BseNI ACTGG 3 cut(s) 147, 224, 928
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 4 cut(s) 632, 687, 715, 1283
BsgI GTGCAG 1 cut(s) 122
BsiHKAI GWGCWC 4 cut(s) 316, 403, 799, 1068
BsiSI CCGG 4 cut(s) 323, 523, 1022, 1247
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 2 cut(s) 178, 724
BsmAI GTCTC 2 cut(s) 943, 972
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 2 cut(s) 262, 829
Bsp1286I GDGCHC 5 cut(s) 316, 403, 553, 799, 1068
Bsp143I GATC 5 cut(s) 30, 123, 325, 622, 958
Bsp19I CCATGG 1 cut(s) 362
BspACI CCGC 1 cut(s) 213
BspCNI CTCAG 2 cut(s) 19, 688
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 360, 422, 423
BspMAI CTGCAG 1 cut(s) 705
BspMI ACCTGC 1 cut(s) 94
BspPI GGATC 3 cut(s) 118, 333, 953
BspTI CTTAAG 1 cut(s) 835
BsrDI GCAATG 3 cut(s) 878, 984, 1227
BsrFI RCCGGY 1 cut(s) 1246
BsrI ACTGG 3 cut(s) 147, 224, 928
BssAI RCCGGY 1 cut(s) 1246
BssECI CCNNGG 3 cut(s) 362, 1058, 1070
BssMI GATC 5 cut(s) 30, 123, 325, 622, 958
BssNAI GTATAC 1 cut(s) 1077
BssT1I CCWWGG 1 cut(s) 362
Bst1107I GTATAC 1 cut(s) 1077
Bst2UI CCWGG 2 cut(s) 165, 1059
Bst4CI ACNGT 2 cut(s) 19, 139
Bst6I CTCTTC 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 835
BstBAI YACGTR 1 cut(s) 1080
BstC8I GCNNGC 2 cut(s) 472, 1167
BstDEI CTNAG 3 cut(s) 27, 675, 937
BstDSI CCRYGG 2 cut(s) 362, 1070
BstF5I GGATG 4 cut(s) 160, 576, 580, 767
BstKTI GATC 5 cut(s) 33, 126, 328, 625, 961
BstMAI GTCTC 2 cut(s) 943, 972
BstMBI GATC 5 cut(s) 30, 123, 325, 622, 958
BstMWI GCNNNNNNNGC 1 cut(s) 1171
BstNI CCWGG 2 cut(s) 165, 1059
BstNSI RCATGY 3 cut(s) 474, 582, 1169
BstSCI CCNGG 2 cut(s) 163, 1057
BstSFI CTRYAG 3 cut(s) 93, 138, 701
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 4 cut(s) 632, 687, 715, 1283
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BstZ17I GTATAC 1 cut(s) 1077
BtgI CCRYGG 2 cut(s) 362, 1070
BtgZI GCGATG 1 cut(s) 253
BtsCI GGATG 4 cut(s) 160, 576, 580, 767
BtsIMutI CAGTG 3 cut(s) 154, 231, 797
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 472, 1167
CciI TCATGA 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 1246
Cfr13I GGNCC 2 cut(s) 359, 421
Csp6I GTAC 2 cut(s) 158, 600
CspCI CAANNNNNGTGG 2 cut(s) 677, 712
CviAII CATG 7 cut(s) 363, 404, 471, 562, 579, 862, 1166
CviQI GTAC 2 cut(s) 158, 600
DdeI CTNAG 3 cut(s) 27, 675, 937
DpnI GATC 5 cut(s) 32, 125, 327, 624, 960
DpnII GATC 5 cut(s) 30, 123, 325, 622, 958
DraI TTTAAA 1 cut(s) 1210
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 2 cut(s) 401, 1066
Eco130I CCWWGG 1 cut(s) 362
Eco24I GRGCYC 2 cut(s) 403, 1068
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 2 cut(s) 359, 421
Eco53kI GAGCTC 2 cut(s) 401, 1066
EcoICRI GAGCTC 2 cut(s) 401, 1066
EcoO109I RGGNCCY 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 163, 1057
EcoRV GATATC 1 cut(s) 897
EcoT14I CCWWGG 1 cut(s) 362
EcoT22I ATGCAT 2 cut(s) 472, 580
EcoT38I GRGCYC 2 cut(s) 403, 1068
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 7 cut(s) 366, 407, 474, 565, 582, 865, 1169
FaqI GGGAC 1 cut(s) 434
FatI CATG 7 cut(s) 362, 403, 470, 561, 578, 861, 1165
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 1 cut(s) 622
FblI GTMKAC 3 cut(s) 141, 1076, 1261
Fnu4HI GCNGC 4 cut(s) 646, 701, 704, 1272
FokI GGATG 4 cut(s) 147, 583, 587, 774
FriOI GRGCYC 2 cut(s) 403, 1068
Fsp4HI GCNGC 4 cut(s) 646, 701, 704, 1272
FspBI CTAG 3 cut(s) 42, 429, 1176
GluI GCNGC 4 cut(s) 646, 701, 704, 1272
GsuI CTGGAG 3 cut(s) 72, 130, 207
HapII CCGG 4 cut(s) 323, 523, 1022, 1247
Hin1II CATG 7 cut(s) 366, 407, 474, 565, 582, 865, 1169
HinfI GANTC 1 cut(s) 143
HpaII CCGG 4 cut(s) 323, 523, 1022, 1247
HphI GGTGA 3 cut(s) 118, 322, 968
Hpy166II GTNNAC 4 cut(s) 142, 1027, 1077, 1262
Hpy188I TCNGA 3 cut(s) 622, 683, 901
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 4 cut(s) 142, 1027, 1077, 1262
HpyAV CCTTC 3 cut(s) 103, 985, 1237
HpyCH4III ACNGT 2 cut(s) 19, 139
HpyCH4IV ACGT 1 cut(s) 1079
HpyF10VI GCNNNNNNNGC 1 cut(s) 1171
HpyF3I CTNAG 3 cut(s) 27, 675, 937
HpySE526I ACGT 1 cut(s) 1079
Hsp92II CATG 7 cut(s) 366, 407, 474, 565, 582, 865, 1169
KflI GGGWCCC 1 cut(s) 421
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 5 cut(s) 30, 123, 325, 622, 958
LmnI GCTCC 4 cut(s) 215, 398, 1063, 1071
Lsp1109I GCAGC 4 cut(s) 632, 687, 715, 1283
LweI GCATC 4 cut(s) 224, 276, 565, 856
MaeI CTAG 3 cut(s) 42, 429, 1176
MaeII ACGT 1 cut(s) 1079
MaeIII GTNAC 3 cut(s) 557, 731, 874
MalI GATC 5 cut(s) 32, 125, 327, 624, 960
MboI GATC 5 cut(s) 30, 123, 325, 622, 958
MboII GAAGA 3 cut(s) 195, 735, 973
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 5 cut(s) 316, 403, 553, 799, 1068
MluCI AATT 9 cut(s) 65, 385, 751, 803, 889, 1038, 1114, 1128, 1231
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 2 cut(s) 38, 548
MnlI CCTC 8 cut(s) 16, 407, 491, 684, 793, 865, 988, 1207
Mph1103I ATGCAT 2 cut(s) 472, 580
MseI TTAA 9 cut(s) 186, 384, 605, 836, 887, 1013, 1113, 1131, 1209
MspCI CTTAAG 1 cut(s) 835
MspI CCGG 4 cut(s) 323, 523, 1022, 1247
MspR9I CCNGG 2 cut(s) 165, 1059
Mva1269I GAATGC 2 cut(s) 262, 829
MvaI CCWGG 2 cut(s) 165, 1059
MwoI GCNNNNNNNGC 1 cut(s) 1171
NcoI CCATGG 1 cut(s) 362
NdeI CATATG 1 cut(s) 532
NdeII GATC 5 cut(s) 30, 123, 325, 622, 958
NlaIII CATG 7 cut(s) 366, 407, 474, 565, 582, 865, 1169
NlaIV GGNNCC 3 cut(s) 360, 422, 423
NsiI ATGCAT 2 cut(s) 472, 580
NspI RCATGY 3 cut(s) 474, 582, 1169
PaeI GCATGC 2 cut(s) 474, 1169
PagI TCATGA 1 cut(s) 403
PaqCI CACCTGC 1 cut(s) 94
PctI GAATGC 2 cut(s) 262, 829
PflFI GACNNNGTC 1 cut(s) 74
PflMI CCANNNNNTGG 2 cut(s) 178, 724
PkrI GCNGC 4 cut(s) 647, 702, 705, 1273
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Ppu21I YACGTR 1 cut(s) 1080
PpuMI RGGWCCY 1 cut(s) 421
PshBI ATTAAT 1 cut(s) 1131
Psp124BI GAGCTC 2 cut(s) 403, 1068
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 2 cut(s) 163, 1057
PspGI CCWGG 2 cut(s) 163, 1057
PspN4I GGNNCC 3 cut(s) 360, 422, 423
PspPI GGNCC 2 cut(s) 359, 421
PspPPI RGGWCCY 1 cut(s) 421
PstI CTGCAG 1 cut(s) 705
PsuI RGATCY 1 cut(s) 325
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 2 cut(s) 159, 601
RsaNI GTAC 2 cut(s) 158, 600
SacI GAGCTC 2 cut(s) 403, 1068
SaqAI TTAA 9 cut(s) 186, 384, 605, 836, 887, 1013, 1113, 1131, 1209
SatI GCNGC 4 cut(s) 646, 701, 704, 1272
Sau3AI GATC 5 cut(s) 30, 123, 325, 622, 958
Sau96I GGNCC 2 cut(s) 359, 421
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 165, 1059
SduI GDGCHC 5 cut(s) 316, 403, 553, 799, 1068
SfaNI GCATC 4 cut(s) 224, 276, 565, 856
SfcI CTRYAG 3 cut(s) 93, 138, 701
SinI GGWCC 2 cut(s) 359, 421
SmlI CTYRAG 1 cut(s) 835
SmoI CTYRAG 1 cut(s) 835
SphI GCATGC 2 cut(s) 474, 1169
Sse9I AATT 9 cut(s) 65, 385, 751, 803, 889, 1038, 1114, 1128, 1231
SsiI CCGC 1 cut(s) 213
SspMI CTAG 3 cut(s) 42, 429, 1176
SstI GAGCTC 2 cut(s) 403, 1068
StyD4I CCNGG 2 cut(s) 163, 1057
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 2 cut(s) 19, 139
TaiI ACGT 1 cut(s) 1082
TaqI TCGA 3 cut(s) 33, 766, 793
TasI AATT 9 cut(s) 65, 385, 751, 803, 889, 1038, 1114, 1128, 1231
TatI WGTACW 1 cut(s) 599
Tru1I TTAA 9 cut(s) 186, 384, 605, 836, 887, 1013, 1113, 1131, 1209
Tru9I TTAA 9 cut(s) 186, 384, 605, 836, 887, 1013, 1113, 1131, 1209
TscAI CASTG 3 cut(s) 154, 231, 804
TseI GCWGC 4 cut(s) 645, 700, 703, 1271
TspDTI ATGAA 5 cut(s) 269, 420, 549, 973, 1116
TspRI CASTG 3 cut(s) 154, 231, 804
Tth111I GACNNNGTC 1 cut(s) 74
Van91I CCANNNNNTGG 2 cut(s) 178, 724
Vha464I CTTAAG 1 cut(s) 835
VpaK11BI GGWCC 2 cut(s) 359, 421
VspI ATTAAT 1 cut(s) 1131
XapI RAATTY 2 cut(s) 803, 889
XceI RCATGY 3 cut(s) 474, 582, 1169
XmiI GTMKAC 3 cut(s) 141, 1076, 1261
XspI CTAG 3 cut(s) 42, 429, 1176
Zsp2I ATGCAT 2 cut(s) 472, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.