Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0002072

Physical Location & Seq

Genomic Coordinates Forward (+)

12891491 .. 12895384

Gene Structure 3894 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0141100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2289 bp

ATGTTTTCGTCTATGGCTACTGCATCTGTTCAAGGTTCTATTGCTGGTGGGCTTGGGGATCCTGATCGAATTTTTTTTGGTGGATTCCCTTATTACTTTAAAGAAGAACAGATCAGGGAGTTGGTAGAGTCCTTCGGACCCCTTCGCAGTTTTGAACTAGTGACAGATGGAGGCAGAGGAAACAACTCAATTGCTGCCTATTGCGTTTACCAGGATGTTTCAGTTACAGACATTGCTTGTCGTGCCCTGAATGGGATTCGAATGGGTGCCCAGACACTGATTGCTAGGCGTGCTAATCATGGTGCCAACCAGCAGCCGGAACCACATTCTTCTTCTCAAGAAAATTGCAGTATTTGCTGGTGTAGCCTGACAACTGGCCAAGGCCAAGCGATCTTTACTGCTGAGTGCTCCCACCGTTTCCACTACCCTTGTATTGCCAACAATGTTCAGCATGGAAATCTTTACTGCCCTGTCTGCAGAGTAAAATGGGACAAGAATAATGTACCTTTCCAAGTTCCTCCTCCTCAACAGACCAGGATCCCTTTCAGGCAGTTCCCAATGCCGCAGCAACCCCATTGGAATTATCCTCACCTCCCATATCAGCAGCCGCATTGGAATCATCCTCAGTACCCATTTGAGCAGCAACCCCAGGACCCTCCTGAGCCACTTACCTTCTCTGATGATGAACCTCTCCAGTCATTGACCTCACCTGTTCAATCCTCCTCCCACCAGAATGTGACAGTTAAGACTTATGCAGAGTTCTCTGCTATCTCTGCTGCGGAGTCTAGCTCAAAGTTTCCTGTCCTTGTCAGCATCCGTGCACCACCCCTTAAGGATTCTGAGGGCCATGGTCGTACTCCCATTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGACTGGCTCGAAGCTTGACCTTGTCAAGCGTGCTGTCAAATTTGTCATAGAAAACTTGGGGTCTTCAGACAGACTTTCTATTGTCTCATTCTCATCAAATTCTAAGAGAGTTCTTCCTCTCCAAAGGATGACTGCTGATGGCCGTGAAAGTGCCATACTAGCCGTCGAGTCTCTTAGAGCAGGTGGTGGAACTAACATTGCCGAAGCACTCAAGAAAGGAACTAAGGTCCTTGAAGATCGGAGCGAAAGGAACCCAGTTTCTAGCATCATCCTCTTATCCGACGGCCAAGATAACTACTGTCGAAGTGGAAGCCAAATTTTGAACCAATTGCCTGCTTCCATTCGTTCTAGTGACATGCAACAGGAAATCCCAGTCCACGCATTTGGGTTTGGCCAAGACCATGATGCAAGCATTATGCATGCTATTTCTGATGCATCTGGTGGTACCTTTTCATTCATTGAATCAGTTGGCATGATACAAGATGGTTTTGCTTTGTGCATTGGTGGTCTTCTCAGTGTAGTGGCTCAGGAACTTCGCCTCACAGTAAGGTCAGCATCAGCTGGGGTGAAGATTGTTTCAATACCATCAGGGAGACATGTCAGCCAGATTTCTGATGAGGGCCAGCAAGGTGTAGTTGATATTGGAAATATGTATGCAGAAGAGGAGAAACAATTTCTGGTCTACCTGTTAGTTCCACCCTCAGCTTCACATGTTAAGATGCCATTGTTAGATGTAACATGCGTGTACAAAGATCTTGCTTCAAATGAGTTGATGCAGGTGCAAGGTGAGAGAGTAGAGATACTGAGACCTGAGGTTTGTTCCCCTGCAGAAAAGGCAGTGTCTTTGGAGGTTGATCGACAGCGGAACAGAATTGTGGTTGCTGAAACTATTGCAGAGGCACAAAGGTTGGCTGAGATGGGAAACCTGGAAGGTGCACGGGCACTTTTGGCTGAACGAAGAGCAACTGTCTTAGCATCAGCAGCAGCTCAAGCTGGGGATGGTCTCAGTAATTTGTTTGAAACTGAGCTTAGAGAAGTCATGGAGAGAATGGCATCTATGGACTTGTACGCTAACTCAGGGCGTGCTTACGCTCTCGCAGGGATGAGTTCTCATTCTCGACAGAGGGCTTCAGCTAGGGGTGACACAATAACCTCATGTCTCTTTGGTGCCTCCAACCGTGTGAATTTCTCTAGCGGTGCCAATGCCTTAATGCAGCAGTCGGGTGCCTCTGCTTGTTTTGGCGCCCCTGCAGGTTTCGGTGGTGCTAGTTTTTCATCCGGTGCCGCCCCAGGTGGAAGTCAATGCTCTTCTGCAGTTGGTGCTTTTGAAACACCTGCTATGGTTAGAATGGTAGAAAAATCACAGAAGAGCAATCAATCTGGGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

762

Amino Acids

82.16

Weight (kDa)

5.91

Isoelectric Point (pI)

52.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	24 - 92	4.6e-08	RNA recognition motif
zf-RING_11	PF17123	115 - 145	2.5e-09	RING-like zinc finger
zf-RING_2	PF13639	115 - 160	7.6e-07	Ring finger domain
VWA_CoxE	PF05762	285 - 402	2.7e-07	VWA domain containing CoxE-like protein
VWA	PF00092	289 - 453	2.6e-24	von Willebrand factor type A domain
VWA_3	PF13768	289 - 452	7.8e-20	von Willebrand factor type A domain
VWA_2	PF13519	290 - 396	8.8e-19	von Willebrand factor type A domain
WAV3_C	PF25243	584 - 650	9.1e-06	E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint	PF14624	640 - 683	4.5e-11	VWA / Hh protein intein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002072)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78635 AT1G78640 AT2G33720
fragaria_vesca	FvH4_3g33791 FvH4_3g37630
malus_domestica	MD14G1106900.v1.1
prunus_persica	Prupe.5G092200_v2.0.a1 Prupe.5G102200_v2.0.a1 Prupe.5G102300_v2.0.a1 Prupe.5G102400_v2.0.a1 Prupe.5G102500_v2.0.a1
pyrus_communis	pycom05g30850 pycom05g30870 pycom14g10060
rosa_chinensis	RchiOBHm_Chr2g0116441
rosa_laevigata	RLG00000003924 RLG00000018270 RLG00000035460 RLG00000035470
rosa_multiflora	Rmu_sc0000338.1_g000013 Rmu_sc0000689.1_g000016 Rmu_sc0001164.1_g000007 Rmu_sc0004191.1_g000056 Rmu_sc0019528.1_g000001
rosa_roxburghii	Rroxscaffold_1G00019310 Rroxscaffold_1G00019370 Rroxscaffold_5G00379640
rosa_rugosa	Rorug05G0141100 Rorug05G0339700
rosa_samantha	Rh2AG262200 Rh2CG268400 Rh2CG268500 Rh2DG270400 Rh5AG402000 Rh5BG019500 Rh5BG414100 Rh5CG438600 Rh5DG428700 Rh7AG166100 Rh7BG168200 Rh7CG174500 Rh7DG167800
rosa_wichuraiana	Rw5G037840 Rw7G014460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	1070, 1666, 2242
Acc36I	ACCTGC	4 cut(s)	1070, 1666, 2141, 2242
Acc65I	GGTACC	1 cut(s)	1343
AccB1I	GGYRCC	8 cut(s)	266, 302, 1343, 2066, 2096, 2123, 2141, 2180
AccI	GTMKAC	1 cut(s)	1581
AciI	CCGC	6 cut(s)	563, 608, 779, 1762, 2094, 2184
AclWI	GGATC	4 cut(s)	53, 66, 532, 545
AcoI	YGGCCR	4 cut(s)	376, 1039, 1183, 1291
AcsI	RAATTY	5 cut(s)	69, 938, 997, 1215, 2083
AcuI	CTGAAG	2 cut(s)	948, 2013
AcyI	GRCGYC	1 cut(s)	2142
AfaI	GTAC	6 cut(s)	504, 629, 856, 1345, 1646, 1967
AfiI	CCNNNNNNNGG	2 cut(s)	252, 316
AflII	CTTAAG	1 cut(s)	830
AflIII	ACRYGT	2 cut(s)	1495, 1609
AhlI	ACTAGT	1 cut(s)	157
AjnI	CCWGG	5 cut(s)	210, 533, 648, 1824, 2188
AluBI	AGCT	8 cut(s)	789, 913, 1460, 1604, 1886, 1892, 1927, 2033
AluI	AGCT	8 cut(s)	789, 913, 1460, 1604, 1886, 1892, 1927, 2033
Alw21I	GWGCWC	3 cut(s)	410, 823, 1837
Alw26I	GTCTC	6 cut(s)	988, 1074, 1486, 1699, 1907, 2063
Alw44I	GTGCAC	2 cut(s)	819, 1833
AlwI	GGATC	4 cut(s)	53, 66, 532, 545
AlwNI	CAGNNNCTG	1 cut(s)	277
AoxI	GGCC	7 cut(s)	376, 382, 844, 1039, 1183, 1291, 1519
ApaLI	GTGCAC	2 cut(s)	819, 1833
ApoI	RAATTY	5 cut(s)	69, 938, 997, 1215, 2083
ArsI	GACNNNNNNTTYG	2 cut(s)	1698, 1730
Asp718I	GGTACC	1 cut(s)	1343
AspLEI	GCGC	1 cut(s)	2144
AspS9I	GGNCC	5 cut(s)	137, 652, 844, 1126, 1519
AsuHPI	GGTGA	5 cut(s)	581, 699, 1477, 1697, 2051
AsuII	TTCGAA	1 cut(s)	259
AvaII	GGWCC	3 cut(s)	137, 652, 1126
AxyI	CCTNAGG	1 cut(s)	1710
BaeGI	GKGCMC	5 cut(s)	247, 271, 823, 1837, 1843
BalI	TGGCCA	2 cut(s)	378, 1293
BamHI	GGATCC	2 cut(s)	58, 537
BanI	GGYRCC	8 cut(s)	266, 302, 1343, 2066, 2096, 2123, 2141, 2180
BbsI	GAAGAC	2 cut(s)	954, 1400
Bbv12I	GWGCWC	3 cut(s)	410, 823, 1837
BbvCI	CCTCAGC	1 cut(s)	1600
BccI	CCATC	6 cut(s)	161, 1031, 1376, 1492, 1810, 1892
BceAI	ACGGC	3 cut(s)	1026, 1046, 1198
BciT130I	CCWGG	5 cut(s)	212, 535, 650, 1826, 2190
BcoDI	GTCTC	6 cut(s)	988, 1074, 1486, 1699, 1907, 2063
BcuI	ACTAGT	1 cut(s)	157
BfaI	CTAG	9 cut(s)	158, 285, 786, 1058, 1161, 1248, 2034, 2091, 2166
BfmI	CTRYAG	4 cut(s)	475, 1725, 2148, 2211
BfoI	RGCGCY	1 cut(s)	2145
BfrI	CTTAAG	1 cut(s)	830
BfuAI	ACCTGC	4 cut(s)	1070, 1666, 2141, 2242
BglII	AGATCT	1 cut(s)	1651
Bme1390I	CCNGG	5 cut(s)	212, 535, 650, 1826, 2190
Bme18I	GGWCC	3 cut(s)	137, 652, 1126
BmgT120I	GGNCC	5 cut(s)	137, 652, 844, 1126, 1519
BmrFI	CCNGG	5 cut(s)	212, 535, 650, 1826, 2190
BmrI	ACTGGG	2 cut(s)	1148, 1265
BmuI	ACTGGG	2 cut(s)	1148, 1265
BpiI	GAAGAC	2 cut(s)	954, 1400
BplI	GAGNNNNNCTC	2 cut(s)	773, 805
BpmI	CTGGAG	1 cut(s)	677
Bpu10I	CCTNAGC	3 cut(s)	660, 1425, 1600
Bpu14I	TTCGAA	1 cut(s)	259
BpuEI	CTTGAG	3 cut(s)	321, 1094, 1872
BsaBI	GATNNNNATC	1 cut(s)	63
BsaHI	GRCGYC	1 cut(s)	2142
BsaI	GGTCTC	2 cut(s)	1699, 1907
BsaJI	CCNNGG	4 cut(s)	379, 648, 847, 2188
BsaWI	WCCGGW	1 cut(s)	2177
Bsc4I	CCNNNNNNNGG	2 cut(s)	252, 316
Bse1I	ACTGG	5 cut(s)	379, 694, 907, 1154, 1271
Bse21I	CCTNAGG	1 cut(s)	1710
Bse3DI	GCAATG	2 cut(s)	231, 1095
Bse8I	GATNNNNATC	1 cut(s)	63
BseBI	CCWGG	5 cut(s)	212, 535, 650, 1826, 2190
BseDI	CCNNGG	4 cut(s)	379, 648, 847, 2188
BseGI	GGATG	9 cut(s)	220, 619, 813, 889, 1032, 1167, 1903, 2007, 2174
BseJI	GATNNNNATC	1 cut(s)	63
BseLI	CCNNNNNNNGG	2 cut(s)	252, 316
BseMI	GCAATG	2 cut(s)	231, 1095
BseNI	ACTGG	5 cut(s)	379, 694, 907, 1154, 1271
BseRI	GAGGAG	4 cut(s)	510, 513, 712, 1577
BseSI	GKGCMC	5 cut(s)	247, 271, 823, 1837, 1843
BseYI	CCCAGC	2 cut(s)	1460, 1892
BshFI	GGCC	7 cut(s)	378, 384, 846, 1041, 1185, 1293, 1521
BshNI	GGYRCC	8 cut(s)	266, 302, 1343, 2066, 2096, 2123, 2141, 2180
BsiHKAI	GWGCWC	3 cut(s)	410, 823, 1837
BsiSI	CCGG	2 cut(s)	317, 2178
BslFI	GGGAC	1 cut(s)	503
BslI	CCNNNNNNNGG	2 cut(s)	252, 316
BsmAI	GTCTC	6 cut(s)	988, 1074, 1486, 1699, 1907, 2063
BsmFI	GGGAC	1 cut(s)	503
BsnI	GGCC	7 cut(s)	378, 384, 846, 1041, 1185, 1293, 1521
Bso31I	GGTCTC	2 cut(s)	1699, 1907
Bsp119I	TTCGAA	1 cut(s)	259
Bsp1286I	GDGCHC	6 cut(s)	247, 271, 410, 823, 1837, 1843
Bsp1407I	TGTACA	1 cut(s)	1644
Bsp143I	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
Bsp19I	CCATGG	1 cut(s)	847
BspACI	CCGC	6 cut(s)	563, 608, 779, 1762, 2094, 2184
BspANI	GGCC	7 cut(s)	378, 384, 846, 1041, 1185, 1293, 1521
BspMAI	CTGCAG	4 cut(s)	479, 1729, 2152, 2215
BspMI	ACCTGC	4 cut(s)	1070, 1666, 2141, 2242
BspPI	GGATC	4 cut(s)	53, 66, 532, 545
BspQI	GCTCTTC	3 cut(s)	1852, 2212, 2261
BspT104I	TTCGAA	1 cut(s)	259
BspT107I	GGYRCC	8 cut(s)	266, 302, 1343, 2066, 2096, 2123, 2141, 2180
BspTI	CTTAAG	1 cut(s)	830
BspTNI	GGTCTC	2 cut(s)	1699, 1907
BsrDI	GCAATG	2 cut(s)	231, 1095
BsrGI	TGTACA	1 cut(s)	1644
BsrI	ACTGG	5 cut(s)	379, 694, 907, 1154, 1271
BssECI	CCNNGG	4 cut(s)	379, 648, 847, 2188
BssMI	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
BssNI	GRCGYC	1 cut(s)	2142
BssT1I	CCWWGG	2 cut(s)	379, 847
Bst2UI	CCWGG	5 cut(s)	212, 535, 650, 1826, 2190
Bst4CI	ACNGT	7 cut(s)	416, 742, 877, 1199, 1444, 1867, 2078
Bst6I	CTCTTC	4 cut(s)	1554, 1852, 2212, 2261
BstACI	GRCGYC	1 cut(s)	2142
BstAFI	CTTAAG	1 cut(s)	830
BstAPI	GCANNNNNTGC	2 cut(s)	354, 2219
BstAUI	TGTACA	1 cut(s)	1644
BstBI	TTCGAA	1 cut(s)	259
BstC8I	GCNNGC	7 cut(s)	291, 930, 1233, 1309, 1320, 1523, 1983
BstDSI	CCRYGG	1 cut(s)	847
BstF5I	GGATG	9 cut(s)	220, 619, 813, 889, 1032, 1167, 1903, 2007, 2174
BstH2I	RGCGCY	1 cut(s)	2145
BstHHI	GCGC	1 cut(s)	2144
BstKTI	GATC	8 cut(s)	61, 67, 114, 393, 540, 1138, 1654, 1756
BstMAI	GTCTC	6 cut(s)	988, 1074, 1486, 1699, 1907, 2063
BstMBI	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
BstNI	CCWGG	5 cut(s)	212, 535, 650, 1826, 2190
BstNSI	RCATGY	5 cut(s)	1258, 1322, 1499, 1613, 1641
BstSCI	CCNGG	5 cut(s)	210, 533, 648, 1824, 2188
BstSFI	CTRYAG	4 cut(s)	475, 1725, 2148, 2211
BstSLI	GKGCMC	5 cut(s)	247, 271, 823, 1837, 1843
BstV2I	GAAGAC	2 cut(s)	954, 1400
BstX2I	RGATCY	3 cut(s)	58, 537, 1651
BstXI	CCANNNNNNTGG	1 cut(s)	1283
BstYI	RGATCY	3 cut(s)	58, 537, 1651
Bsu36I	CCTNAGG	1 cut(s)	1710
BsuRI	GGCC	7 cut(s)	378, 384, 846, 1041, 1185, 1293, 1521
BtgI	CCRYGG	1 cut(s)	847
BtsCI	GGATG	9 cut(s)	220, 619, 813, 889, 1032, 1167, 1903, 2007, 2174
BtsI	GCAGTG	1 cut(s)	1743
BtsIMutI	CAGTG	3 cut(s)	275, 1420, 1743
BveI	ACCTGC	4 cut(s)	1070, 1666, 2141, 2242
Cac8I	GCNNGC	7 cut(s)	291, 930, 1233, 1309, 1320, 1523, 1983
CaiI	CAGNNNCTG	1 cut(s)	277
CfoI	GCGC	1 cut(s)	2144
Cfr13I	GGNCC	5 cut(s)	137, 652, 844, 1126, 1519
Csp6I	GTAC	6 cut(s)	503, 628, 855, 1344, 1645, 1966
CspCI	CAANNNNNGTGG	2 cut(s)	410, 445
CviQI	GTAC	6 cut(s)	503, 628, 855, 1344, 1645, 1966
DinI	GGCGCC	1 cut(s)	2143
DpnI	GATC	8 cut(s)	60, 66, 113, 392, 539, 1137, 1653, 1755
DpnII	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
DraI	TTTAAA	1 cut(s)	100
EaeI	YGGCCR	4 cut(s)	376, 1039, 1183, 1291
Eam1104I	CTCTTC	4 cut(s)	1554, 1852, 2212, 2261
EarI	CTCTTC	4 cut(s)	1554, 1852, 2212, 2261
Eco130I	CCWWGG	2 cut(s)	379, 847
Eco31I	GGTCTC	2 cut(s)	1699, 1907
Eco47I	GGWCC	3 cut(s)	137, 652, 1126
Eco57I	CTGAAG	2 cut(s)	948, 2013
Eco81I	CCTNAGG	1 cut(s)	1710
EcoO109I	RGGNCCY	2 cut(s)	652, 1126
EcoRII	CCWGG	5 cut(s)	210, 533, 648, 1824, 2188
EcoT14I	CCWWGG	2 cut(s)	379, 847
EcoT22I	ATGCAT	2 cut(s)	1320, 1336
EgeI	GGCGCC	1 cut(s)	2143
EheI	GGCGCC	1 cut(s)	2143
ErhI	CCWWGG	2 cut(s)	379, 847
FaqI	GGGAC	1 cut(s)	503
FblI	GTMKAC	1 cut(s)	1581
FokI	GGATG	9 cut(s)	227, 606, 800, 896, 1039, 1154, 1910, 2014, 2161
FspBI	CTAG	9 cut(s)	158, 285, 786, 1058, 1161, 1248, 2034, 2091, 2166
GlaI	GCGC	1 cut(s)	2143
GsaI	CCCAGC	2 cut(s)	1464, 1896
GsuI	CTGGAG	1 cut(s)	677
HaeII	RGCGCY	1 cut(s)	2145
HaeIII	GGCC	7 cut(s)	378, 384, 846, 1041, 1185, 1293, 1521
HapII	CCGG	2 cut(s)	317, 2178
HhaI	GCGC	1 cut(s)	2144
Hin1I	GRCGYC	1 cut(s)	2142
Hin6I	GCGC	1 cut(s)	2142
HinP1I	GCGC	1 cut(s)	2142
HindIII	AAGCTT	1 cut(s)	911
HinfI	GANTC	8 cut(s)	84, 128, 256, 616, 782, 836, 1067, 1361
HpaII	CCGG	2 cut(s)	317, 2178
HphI	GGTGA	5 cut(s)	581, 699, 1477, 1697, 2051
Hpy166II	GTNNAC	6 cut(s)	208, 821, 1276, 1582, 1645, 1835
Hpy188I	TCNGA	8 cut(s)	137, 679, 841, 967, 1140, 1180, 1330, 1513
Hpy188III	TCNNGA	7 cut(s)	62, 338, 659, 881, 1111, 1427, 2016
Hpy8I	GTNNAC	6 cut(s)	208, 821, 1276, 1582, 1645, 1835
Hpy99I	CGWCG	2 cut(s)	1067, 1184
HpyAV	CCTTC	4 cut(s)	142, 152, 682, 1823
HpyCH4III	ACNGT	7 cut(s)	416, 742, 877, 1199, 1444, 1867, 2078
Hsp92I	GRCGYC	1 cut(s)	2142
HspAI	GCGC	1 cut(s)	2142
KasI	GGCGCC	1 cut(s)	2141
KpnI	GGTACC	1 cut(s)	1347
Kzo9I	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
LguI	GCTCTTC	3 cut(s)	1852, 2212, 2261
LmnI	GCTCC	2 cut(s)	413, 1140
MaeI	CTAG	9 cut(s)	158, 285, 786, 1058, 1161, 1248, 2034, 2091, 2166
MaeIII	GTNAC	7 cut(s)	160, 223, 736, 871, 1250, 1633, 2039
MalI	GATC	8 cut(s)	60, 66, 113, 392, 539, 1137, 1653, 1755
MboI	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
MfeI	CAATTG	2 cut(s)	189, 1226
MflI	RGATCY	3 cut(s)	58, 537, 1651
MhlI	GDGCHC	6 cut(s)	247, 271, 410, 823, 1837, 1843
MlsI	TGGCCA	2 cut(s)	378, 1293
MluNI	TGGCCA	2 cut(s)	378, 1293
Mly113I	GGCGCC	1 cut(s)	2142
MlyI	GAGTC	3 cut(s)	137, 791, 1076
MmeI	TCCRAC	2 cut(s)	1203, 2097
Mox20I	TGGCCA	2 cut(s)	378, 1293
Mph1103I	ATGCAT	2 cut(s)	1320, 1336
MscI	TGGCCA	2 cut(s)	378, 1293
MseI	TTAA	5 cut(s)	99, 744, 831, 1614, 2108
MslI	CAYNNNNRTG	1 cut(s)	732
Msp20I	TGGCCA	2 cut(s)	378, 1293
MspA1I	CMGCKG	2 cut(s)	1460, 1762
MspCI	CTTAAG	1 cut(s)	830
MspI	CCGG	2 cut(s)	317, 2178
MspR9I	CCNGG	5 cut(s)	212, 535, 650, 1826, 2190
MunI	CAATTG	2 cut(s)	189, 1226
MvaI	CCWGG	5 cut(s)	212, 535, 650, 1826, 2190
NarI	GGCGCC	1 cut(s)	2142
NcoI	CCATGG	1 cut(s)	847
NdeII	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
NmuCI	GTSAC	4 cut(s)	160, 736, 1250, 2039
NsiI	ATGCAT	2 cut(s)	1320, 1336
NspI	RCATGY	5 cut(s)	1258, 1322, 1499, 1613, 1641
NspV	TTCGAA	1 cut(s)	259
PaeI	GCATGC	1 cut(s)	1322
PaqCI	CACCTGC	3 cut(s)	1070, 1666, 2242
PciI	ACATGT	2 cut(s)	1495, 1609
PciSI	GCTCTTC	3 cut(s)	1852, 2212, 2261
PfeI	GAWTC	5 cut(s)	84, 256, 616, 836, 1361
PflFI	GACNNNGTC	1 cut(s)	920
PleI	GAGTC	3 cut(s)	136, 790, 1075
PluTI	GGCGCC	1 cut(s)	2145
PpsI	GAGTC	3 cut(s)	136, 790, 1075
PpuMI	RGGWCCY	2 cut(s)	652, 1126
PscI	ACATGT	2 cut(s)	1495, 1609
Psp5II	RGGWCCY	2 cut(s)	652, 1126
Psp6I	CCWGG	5 cut(s)	210, 533, 648, 1824, 2188
PspFI	CCCAGC	2 cut(s)	1460, 1892
PspGI	CCWGG	5 cut(s)	210, 533, 648, 1824, 2188
PspPI	GGNCC	5 cut(s)	137, 652, 844, 1126, 1519
PspPPI	RGGWCCY	2 cut(s)	652, 1126
PstI	CTGCAG	4 cut(s)	479, 1729, 2152, 2215
PstNI	CAGNNNCTG	1 cut(s)	277
PsuI	RGATCY	3 cut(s)	58, 537, 1651
PsyI	GACNNNGTC	1 cut(s)	920
PvuII	CAGCTG	1 cut(s)	1460
RsaI	GTAC	6 cut(s)	504, 629, 856, 1345, 1646, 1967
RsaNI	GTAC	6 cut(s)	503, 628, 855, 1344, 1645, 1966
RseI	CAYNNNNRTG	1 cut(s)	732
SapI	GCTCTTC	3 cut(s)	1852, 2212, 2261
SaqAI	TTAA	5 cut(s)	99, 744, 831, 1614, 2108
Sau3AI	GATC	8 cut(s)	58, 64, 111, 390, 537, 1135, 1651, 1753
Sau96I	GGNCC	5 cut(s)	137, 652, 844, 1126, 1519
SbfI	CCTGCAGG	1 cut(s)	2152
SchI	GAGTC	3 cut(s)	137, 791, 1076
ScrFI	CCNGG	5 cut(s)	212, 535, 650, 1826, 2190
SdaI	CCTGCAGG	1 cut(s)	2152
SduI	GDGCHC	6 cut(s)	247, 271, 410, 823, 1837, 1843
SfcI	CTRYAG	4 cut(s)	475, 1725, 2148, 2211
SfoI	GGCGCC	1 cut(s)	2143
SfuI	TTCGAA	1 cut(s)	259
SinI	GGWCC	3 cut(s)	137, 652, 1126
SmiMI	CAYNNNNRTG	1 cut(s)	732
SmlI	CTYRAG	4 cut(s)	336, 830, 1109, 1887
SmoI	CTYRAG	4 cut(s)	336, 830, 1109, 1887
SpeI	ACTAGT	1 cut(s)	157
SphI	GCATGC	1 cut(s)	1322
Sse8387I	CCTGCAGG	1 cut(s)	2152
SsiI	CCGC	6 cut(s)	563, 608, 779, 1762, 2094, 2184
SspDI	GGCGCC	1 cut(s)	2141
SspMI	CTAG	9 cut(s)	158, 285, 786, 1058, 1161, 1248, 2034, 2091, 2166
StyD4I	CCNGG	5 cut(s)	210, 533, 648, 1824, 2188
StyI	CCWWGG	2 cut(s)	379, 847
TaaI	ACNGT	7 cut(s)	416, 742, 877, 1199, 1444, 1867, 2078
TaqI	TCGA	7 cut(s)	67, 259, 908, 1065, 1201, 1756, 2017
TatI	WGTACW	1 cut(s)	1644
TauI	GCSGC	3 cut(s)	565, 610, 2186
TfiI	GAWTC	5 cut(s)	84, 256, 616, 836, 1361
Tru1I	TTAA	5 cut(s)	99, 744, 831, 1614, 2108
Tru9I	TTAA	5 cut(s)	99, 744, 831, 1614, 2108
TscAI	CASTG	3 cut(s)	282, 1420, 1743
TseFI	GTSAC	4 cut(s)	160, 736, 1250, 2039
Tsp45I	GTSAC	4 cut(s)	160, 736, 1250, 2039
TspDTI	ATGAA	4 cut(s)	699, 1341, 1345, 2163
TspGWI	ACGGA	1 cut(s)	806
TspRI	CASTG	3 cut(s)	282, 1420, 1743
Tth111I	GACNNNGTC	1 cut(s)	920
Vha464I	CTTAAG	1 cut(s)	830
VneI	GTGCAC	2 cut(s)	819, 1833
VpaK11BI	GGWCC	3 cut(s)	137, 652, 1126
XapI	RAATTY	5 cut(s)	69, 938, 997, 1215, 2083
XceI	RCATGY	5 cut(s)	1258, 1322, 1499, 1613, 1641
XmiI	GTMKAC	1 cut(s)	1581
XspI	CTAG	9 cut(s)	158, 285, 786, 1058, 1161, 1248, 2034, 2091, 2166
Zsp2I	ATGCAT	2 cut(s)	1320, 1336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.