FvH4_3g37630

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002072
Physical Location & Seq
Forward (+)
32259590 .. 32260102
513 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37630.t1

Sequence Viewer

Length: 513 bp
ATGGCTGAAACACTTCTCTTTCCTTCTCTGTTTGCTTCAACAAGGGTGCAAGACCAACCCTGTGCACTCAACCAAACTGACAACAGAGGTCTCAATCTGGGGCTAAGTCTGGGCTCCATTGACTACGACCCGTGGTTCATCAAGAAGAAGCTGAAGGTCAGCGACCTCGGCGACCTGAGCAGGCTTTTGGTGCCCAAGAGATTAACCAAGAAACATGTTTTGCCGTTGATGCACAAGAGCTTGGACGAAAGTGTCCACAGTAGCAATGGGGGAGCGGTCACCGTTTACGACAGTGATATGAAAACGAAGCATGAGTTGGTCTTCAAATACTGGCCATCGTCAGAGAGTTACGTTCTGCAAAGAAACTGGAAGAAGGACTTTGTGAATGCGAGGAAGTTGAAGGAAGGTGATCTAATTGGGCTCTATTGGGATGCTCGCAGTTCTGCTTTTCTATTTTCGCTTCTTCAACGAGAAGTGGAGATCGATACTACTCTGGGATATTGGAGGAATTAA

Protein Analysis

171

Amino Acids

19.54

Weight (kDa)

9.12

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 49 - 155 7.6e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 190
AccBSI CCGCTC 1 cut(s) 275
AciI CCGC 1 cut(s) 275
AcoI YGGCCR 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 4 cut(s) 39, 325, 400, 467
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 2 cut(s) 151, 240
AluI AGCT 2 cut(s) 151, 240
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 95
Alw44I GTGCAC 1 cut(s) 63
AoxI GGCC 1 cut(s) 332
ApaLI GTGCAC 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 12
AsuHPI GGTGA 2 cut(s) 271, 419
BaeGI GKGCMC 2 cut(s) 67, 195
BalI TGGCCA 1 cut(s) 334
BanI GGYRCC 1 cut(s) 190
BanII GRGCYC 2 cut(s) 116, 423
BbsI GAAGAC 1 cut(s) 313
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 1 cut(s) 343
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 115, 192
BmsI GCATC 2 cut(s) 219, 421
BpiI GAAGAC 1 cut(s) 313
Bpu10I CCTNAGC 1 cut(s) 176
Bsa29I ATCGAT 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 131, 166
Bse1I ACTGG 2 cut(s) 335, 371
Bse3DI GCAATG 1 cut(s) 271
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 2 cut(s) 131, 166
BseGI GGATG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 2 cut(s) 335, 371
BseSI GKGCMC 2 cut(s) 67, 195
BshFI GGCC 1 cut(s) 334
BshNI GGYRCC 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 1 cut(s) 334
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 4 cut(s) 67, 116, 195, 423
Bsp143I GATC 2 cut(s) 409, 480
BspACI CCGC 1 cut(s) 275
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 1 cut(s) 168
BspDI ATCGAT 1 cut(s) 483
BspLI GGNNCC 2 cut(s) 115, 192
BspT107I GGYRCC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 271
BsrI ACTGG 2 cut(s) 335, 371
BssECI CCNNGG 2 cut(s) 131, 166
BssMI GATC 2 cut(s) 409, 480
Bst4CI ACNGT 3 cut(s) 260, 283, 293
BstC8I GCNNGC 2 cut(s) 182, 436
BstDEI CTNAG 2 cut(s) 104, 176
BstDSI CCRYGG 1 cut(s) 131
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 1 cut(s) 436
BstKTI GATC 2 cut(s) 412, 483
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 2 cut(s) 409, 480
BstMWI GCNNNNNNNGC 4 cut(s) 168, 177, 190, 229
BstNSI RCATGY 1 cut(s) 218
BstPI GGTNACC 1 cut(s) 277
BstSLI GKGCMC 2 cut(s) 67, 195
BstV2I GAAGAC 1 cut(s) 313
Bsu15I ATCGAT 1 cut(s) 483
BsuRI GGCC 1 cut(s) 334
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 131
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 182, 436
ClaI ATCGAT 1 cut(s) 483
CviAII CATG 2 cut(s) 215, 311
CviJI RGCY 8 cut(s) 5, 103, 114, 151, 184, 240, 334, 421
CviKI_1 RGCY 8 cut(s) 5, 103, 114, 151, 184, 240, 334, 421
DdeI CTNAG 2 cut(s) 104, 176
DpnI GATC 2 cut(s) 411, 482
DpnII GATC 2 cut(s) 409, 480
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 332
Eco24I GRGCYC 2 cut(s) 116, 423
Eco31I GGTCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 173
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 116, 423
FaeI CATG 2 cut(s) 218, 314
FaiI YATR 3 cut(s) 216, 299, 312
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FatI CATG 2 cut(s) 214, 310
FokI GGATG 1 cut(s) 443
FriOI GRGCYC 2 cut(s) 116, 423
HaeIII GGCC 1 cut(s) 334
Hin1II CATG 2 cut(s) 218, 314
HphI GGTGA 2 cut(s) 271, 419
Hpy166II GTNNAC 3 cut(s) 65, 256, 286
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 1 cut(s) 142
Hpy8I GTNNAC 3 cut(s) 65, 256, 286
HpyAV CCTTC 5 cut(s) 33, 148, 367, 394, 398
HpyCH4III ACNGT 3 cut(s) 260, 283, 293
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 49, 65, 232, 358
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 177, 190, 229
HpyF3I CTNAG 2 cut(s) 104, 176
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 2 cut(s) 218, 314
Kzo9I GATC 2 cut(s) 409, 480
LmnI GCTCC 2 cut(s) 119, 272
LpnPI CCDG 8 cut(s) 73, 83, 95, 166, 188, 316, 352, 479
LweI GCATC 2 cut(s) 219, 421
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 2 cut(s) 277, 347
MalI GATC 2 cut(s) 411, 482
MbiI CCGCTC 1 cut(s) 275
MboI GATC 2 cut(s) 409, 480
MboII GAAGA 4 cut(s) 157, 313, 382, 455
MhlI GDGCHC 4 cut(s) 67, 116, 195, 423
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 2 cut(s) 414, 508
MluNI TGGCCA 1 cut(s) 334
MnlI CCTC 4 cut(s) 80, 176, 384, 498
Mox20I TGGCCA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 12
MscI TGGCCA 1 cut(s) 334
MseI TTAA 2 cut(s) 203, 511
Msp20I TGGCCA 1 cut(s) 334
Mva1269I GAATGC 1 cut(s) 391
MwoI GCNNNNNNNGC 4 cut(s) 168, 177, 190, 229
NdeII GATC 2 cut(s) 409, 480
NlaIII CATG 2 cut(s) 218, 314
NlaIV GGNNCC 2 cut(s) 115, 192
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 1 cut(s) 277
NspI RCATGY 1 cut(s) 218
PciI ACATGT 1 cut(s) 214
PctI GAATGC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 214
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 115, 192
SaqAI TTAA 2 cut(s) 203, 511
Sau3AI GATC 2 cut(s) 409, 480
SduI GDGCHC 4 cut(s) 67, 116, 195, 423
SetI ASST 8 cut(s) 91, 153, 159, 168, 177, 242, 354, 409
SfaNI GCATC 2 cut(s) 219, 421
Sse9I AATT 2 cut(s) 414, 508
SsiI CCGC 1 cut(s) 275
TaaI ACNGT 3 cut(s) 260, 283, 293
TaiI ACGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 483
TasI AATT 2 cut(s) 414, 508
Tru1I TTAA 2 cut(s) 203, 511
Tru9I TTAA 2 cut(s) 203, 511
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 2 cut(s) 127, 314
TspRI CASTG 1 cut(s) 298
VneI GTGCAC 1 cut(s) 63
XceI RCATGY 1 cut(s) 218
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.