Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0006806.1

Orthogroup ID

OG0013505

Physical Location & Seq

Genomic Coordinates Reverse (-)

132626 .. 134653

Gene Structure 2028 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2028 bp

atgctagtatctccatgtccaactccttcttcttccttctcatttccatcactaccccccaccaaaccccaaaattacaccgccacacccttcaccttcaccttcaccacccacaaacccagactccgctccccccttatctcctccgcctccaaagatgtctggagaagaacaccaccccaccccaaaacgacgccgtcccatccctcccaaccctccccccgccaccgccgcaagctccaaggcacctccttcctggaccacagcgtcgacatggacgacctcaccgcctcaatccgcgccacccaaaacgagcacgacctcaactccctcctctccccctacaagtcccgcaacctctccatccgcttcatggtctccctcctctcccgcgagcccgactggcgccgctccctcgccatcctcgactggatcaacgacgtcgccctgtactctccctccgtcttcgcctacaacgtcgtcatccgcaacgtcctccgcgccaagcagtggcaagtcgcccacggcctgttcgacgaaatgcgccacagagctctggcgccggaccgctacacttactccactctcatcaccgcgtttgggaagtccggcatgttcgacgccgcgctctcgtggctgcagaagatggaggaggaccgggtgtccggggacctggtgctgtatagcaacttgattgagctgtctaggaaactgtgtgattatagcaaagcaatttcgatattttccaggctgaagaggttggggataatgccggatttggtggcttataactcgatgatcaatgtgtttggtaaggccaagttgtttagggaggccagggttttgctgaaggagatgagggcggtgggggttttgccggatactgttagctactcgacgcttttgacaatgtacattgagaatgagaagtttgtggaggcgttggctgtgttctcggagatgaatgaggtcaagtgcccgcttgatctcacgacgtgtaatattatgattgatgtttatgggcagctggatatggcgaaggaggctgataggttgttttggagcatgaggaagatggggcttgagccgaatgttgttagttacaacactcttttgagggtttatggcgatgctgagcttttcggggaagccattcatctttttcgaatgatgcagagaatggatgtggagcagaatgttgtcacgtacaatacgatgattaggatttatgggaagtcgcttgagcatgaaaaggctacaaatcttgtgcaggaaatgcagaagagggggattcagccgaatgccattacatactcgacgattatatctatatggggtagggctgggaaattggacagggcggcaatgctgtttcaaaagctgaggagctctggggttgagattgatcaggttctttatcagacaatgattgtatcttatgagagagtaggtttggttgctcatgctaagcgcttgcttcatgagcttaagcgcccagacaatatccctagggagactgcaattaccattcttgctggagccggtcgtgtagaagaggctacatgggttttccgtcaggctttcgaggctggggaggtgaaggatatagctgtatttggttgtatgattgagcttttctcaagaaaccggaagtatgcaaattgcattgaagtgtttgaaaatatgagagtggcaggatactttcctgcttcccatgtgattggtctagttctcaacgcatatggaaagttacgggagtttgagaaggctgatgctgtatatagggagatgcaggaagaagggtgtgttttctccgatgaaatccacttccaaatgctcagtctttatggtgcaagaaagaactttaagacggtagaggcaatgtttgagaggctggtctccgatcccaacatcaacaaaaaggagttgcatcttgttgttgcaggcatctatgaaagatcaaacagacttaatgattcataccggatcatgaacaggatgaatgaaagtcgagctttgaaatcatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0031967 GO:0031975 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

675

Amino Acids

77.4

Weight (kDa)

9.21

Isoelectric Point (pI)

36.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	153 - 202	2.8e-13	PPR repeat family
PPR	PF01535	191 - 220	1.1e-06	PPR repeat
PPR_3	PF13812	213 - 272	4.9e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	243 - 326	6.5e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	255 - 287	5.2e-06	PPR repeat
PPR_2	PF13041	258 - 304	4.5e-11	PPR repeat family
PPR	PF01535	261 - 290	2.7e-06	PPR repeat
PPR_3	PF13812	282 - 342	5.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	293 - 339	4e-10	PPR repeat family
PPR_3	PF13812	328 - 377	2.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	329 - 375	2.3e-10	PPR repeat family
PPR_long	PF17177	351 - 512	7.6e-12	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	363 - 408	2.3e-11	PPR repeat family
PPR_3	PF13812	387 - 446	5.9e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	399 - 443	6.3e-15	PPR repeat family
PPR	PF01535	401 - 431	5.1e-07	PPR repeat
PPR_1	PF12854	429 - 460	2.9e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013505)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39980
fragaria_vesca	FvH4_6g49520
malus_domestica	MD09G1044200.v1.1
prunus_persica	Prupe.3G273600_v2.0.a1
pyrus_communis	pycom111g03450
rosa_chinensis	RchiOBHm_Chr2g0169431
rosa_laevigata	RLG00000021863
rosa_multiflora	Rmu_co8342305.1_g000001 Rmu_sc0004665.1_g000014 Rmu_sc0004665.1_g000015 Rmu_sc0006806.1_g000040
rosa_roxburghii	Rroxscaffold_2G00082130 Rroxscaffold_3G00240790
rosa_samantha	Rh2AG611300 Rh2BG624900 Rh2CG593300 Rh2DG635500
rosa_wichuraiana	Rw2G050730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	789
AasI	GACNNNNNNGTC	1 cut(s)	266
AatII	GACGTC	1 cut(s)	444
AccB1I	GGYRCC	3 cut(s)	245, 405, 559
AccB7I	CCANNNNNTGG	1 cut(s)	510
AccBSI	CCGCTC	2 cut(s)	129, 411
AccI	GTMKAC	1 cut(s)	270
AccII	CGCG	5 cut(s)	300, 393, 501, 596, 626
AclWI	GGATC	3 cut(s)	440, 1898, 1994
AcuI	CTGAAG	2 cut(s)	773, 869
AcyI	GRCGYC	5 cut(s)	194, 406, 441, 560, 621
AdeI	CACNNNGTG	1 cut(s)	996
AfaI	GTAC	3 cut(s)	452, 914, 1208
AfeI	AGCGCT	1 cut(s)	1472
AfiI	CCNNNNNNNGG	3 cut(s)	373, 510, 600
AflII	CTTAAG	1 cut(s)	1487
AflIII	ACRYGT	1 cut(s)	995
AgsI	TTSAA	4 cut(s)	1376, 1670, 1679, 2020
AhdI	GACNNNNNGTC	1 cut(s)	661
AjiI	CACGTC	1 cut(s)	996
AjnI	CCWGG	4 cut(s)	255, 672, 746, 836
AjuI	GAANNNNNNNTTGG	2 cut(s)	13, 45
Alw21I	GWGCWC	3 cut(s)	318, 556, 1391
Alw26I	GTCTC	3 cut(s)	382, 1508, 1903
AlwI	GGATC	3 cut(s)	440, 1898, 1994
Aor51HI	AGCGCT	1 cut(s)	1472
AoxI	GGCC	3 cut(s)	526, 816, 834
ApeKI	GCWGC	2 cut(s)	637, 1024
ArsI	GACNNNNNNTTYG	2 cut(s)	1825, 1857
Asp700I	GAANNNNTTC	1 cut(s)	1152
AspA2I	CCTAGG	1 cut(s)	1508
AspLEI	GCGC	8 cut(s)	302, 408, 503, 546, 562, 628, 1473, 1494
AspS9I	GGNCC	4 cut(s)	259, 565, 655, 670
AsuC2I	CCSGG	2 cut(s)	659, 667
AsuHPI	GGTGA	6 cut(s)	85, 91, 97, 277, 583, 1609
AsuII	TTCGAA	1 cut(s)	1165
AvaII	GGWCC	4 cut(s)	259, 565, 655, 670
AvrII	CCTAGG	1 cut(s)	1508
BaeGI	GKGCMC	1 cut(s)	980
BanI	GGYRCC	3 cut(s)	245, 405, 559
BanII	GRGCYC	3 cut(s)	399, 556, 1391
BauI	CACGAG	1 cut(s)	631
BbsI	GAAGAC	1 cut(s)	457
Bbv12I	GWGCWC	3 cut(s)	318, 556, 1391
BbvCI	CCTCAGC	1 cut(s)	1382
BbvI	GCAGC	2 cut(s)	624, 1036
BccI	CCATC	6 cut(s)	55, 210, 371, 428, 640, 1069
BceAI	ACGGC	2 cut(s)	181, 541
BciT130I	CCWGG	4 cut(s)	257, 674, 748, 838
BciVI	GTATCC	2 cut(s)	874, 1691
BclI	TGATCA	2 cut(s)	798, 1405
BcnI	CCSGG	2 cut(s)	659, 667
BcoDI	GTCTC	3 cut(s)	382, 1508, 1903
BfaI	CTAG	4 cut(s)	5, 705, 1509, 1727
BfmI	CTRYAG	1 cut(s)	638
BfoI	RGCGCY	4 cut(s)	409, 563, 1474, 1495
BfrI	CTTAAG	1 cut(s)	1487
BfuI	GTATCC	2 cut(s)	874, 1691
BglI	GCCNNNNNGGC	1 cut(s)	403
BisI	GCNGC	6 cut(s)	232, 409, 624, 638, 1025, 1362
BlnI	CCTAGG	1 cut(s)	1508
BlpI	GCTNAGC	2 cut(s)	1134, 1467
BlsI	GCNGC	6 cut(s)	233, 410, 625, 639, 1026, 1363
Bme1390I	CCNGG	6 cut(s)	257, 659, 667, 674, 748, 838
Bme18I	GGWCC	4 cut(s)	259, 565, 655, 670
BmeRI	GACNNNNNGTC	1 cut(s)	661
BmgBI	CACGTC	1 cut(s)	996
BmgT120I	GGNCC	4 cut(s)	259, 565, 655, 670
BmiI	GGNNCC	5 cut(s)	247, 407, 561, 671, 1540
BmrFI	CCNGG	6 cut(s)	257, 659, 667, 674, 748, 838
BmsI	GCATC	6 cut(s)	1120, 1161, 1762, 1779, 1939, 1956
BpiI	GAAGAC	1 cut(s)	457
BplI	GAGNNNNNCTC	2 cut(s)	1622, 1654
BpmI	CTGGAG	2 cut(s)	184, 1557
Bpu10I	CCTNAGC	1 cut(s)	1382
Bpu1102I	GCTNAGC	2 cut(s)	1134, 1467
Bpu14I	TTCGAA	1 cut(s)	1165
BpuEI	CTTGAG	3 cut(s)	1103, 1262, 1624
BpuMI	CCSGG	2 cut(s)	659, 667
BsaAI	YACGTR	1 cut(s)	1206
BsaHI	GRCGYC	5 cut(s)	194, 406, 441, 560, 621
BsaI	GGTCTC	2 cut(s)	382, 1903
BsaJI	CCNNGG	5 cut(s)	241, 523, 666, 837, 1508
BsaWI	WCCGGW	2 cut(s)	1647, 1983
BsaXI	ACNNNNNCTCC	8 cut(s)	108, 138, 311, 341, 443, 473, 563, 593
Bsc4I	CCNNNNNNNGG	3 cut(s)	373, 510, 600
Bse118I	RCCGGY	1 cut(s)	1541
Bse1I	ACTGG	2 cut(s)	407, 434
Bse3DI	GCAATG	2 cut(s)	1371, 1887
BseBI	CCWGG	4 cut(s)	257, 674, 748, 838
BseDI	CCNNGG	5 cut(s)	241, 523, 666, 837, 1508
BseGI	GGATG	6 cut(s)	202, 363, 420, 483, 1189, 2004
BseLI	CCNNNNNNNGG	3 cut(s)	373, 510, 600
BseMI	GCAATG	2 cut(s)	1371, 1887
BseMII	CTCAG	3 cut(s)	1125, 1373, 1852
BseNI	ACTGG	2 cut(s)	407, 434
BseRI	GAGGAG	5 cut(s)	133, 323, 374, 665, 1399
BseSI	GKGCMC	1 cut(s)	980
BseXI	GCAGC	2 cut(s)	624, 1036
BseYI	CCCAGC	2 cut(s)	1343, 1589
BsgI	GTGCAG	1 cut(s)	1289
Bsh1236I	CGCG	5 cut(s)	300, 393, 501, 596, 626
Bsh1285I	CGRYCG	1 cut(s)	1546
BshFI	GGCC	3 cut(s)	528, 818, 836
BshNI	GGYRCC	3 cut(s)	245, 405, 559
BsiEI	CGRYCG	1 cut(s)	1546
BsiHKAI	GWGCWC	3 cut(s)	318, 556, 1391
BsiSI	CCGG	9 cut(s)	563, 609, 658, 666, 773, 878, 1542, 1648, 1984
BslFI	GGGAC	3 cut(s)	184, 334, 683
BslI	CCNNNNNNNGG	3 cut(s)	373, 510, 600
BsmAI	GTCTC	3 cut(s)	382, 1508, 1903
BsmFI	GGGAC	3 cut(s)	184, 334, 683
BsmI	GAATGC	1 cut(s)	1306
BsnI	GGCC	3 cut(s)	528, 818, 836
Bso31I	GGTCTC	2 cut(s)	382, 1903
Bsp119I	TTCGAA	1 cut(s)	1165
Bsp1286I	GDGCHC	5 cut(s)	318, 399, 556, 980, 1391
Bsp1407I	TGTACA	1 cut(s)	912
Bsp143I	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
Bsp1720I	GCTNAGC	2 cut(s)	1134, 1467
BspANI	GGCC	3 cut(s)	528, 818, 836
BspCNI	CTCAG	3 cut(s)	1126, 1374, 1851
BspFNI	CGCG	5 cut(s)	300, 393, 501, 596, 626
BspHI	TCATGA	3 cut(s)	1480, 1989, 2024
BspLI	GGNNCC	5 cut(s)	247, 407, 561, 671, 1540
BspMAI	CTGCAG	1 cut(s)	642
BspPI	GGATC	3 cut(s)	440, 1898, 1994
BspT104I	TTCGAA	1 cut(s)	1165
BspT107I	GGYRCC	3 cut(s)	245, 405, 559
BspTI	CTTAAG	1 cut(s)	1487
BspTNI	GGTCTC	2 cut(s)	382, 1903
BsrBI	CCGCTC	2 cut(s)	129, 411
BsrDI	GCAATG	2 cut(s)	1371, 1887
BsrFI	RCCGGY	1 cut(s)	1541
BsrGI	TGTACA	1 cut(s)	912
BsrI	ACTGG	2 cut(s)	407, 434
BssAI	RCCGGY	1 cut(s)	1541
BssECI	CCNNGG	5 cut(s)	241, 523, 666, 837, 1508
BssMI	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
BssNI	GRCGYC	5 cut(s)	194, 406, 441, 560, 621
BssSI	CACGAG	1 cut(s)	631
BssT1I	CCWWGG	2 cut(s)	241, 1508
Bst2BI	CACGAG	1 cut(s)	631
Bst2UI	CCWGG	4 cut(s)	257, 674, 748, 838
Bst4CI	ACNGT	3 cut(s)	714, 886, 1873
Bst6I	CTCTTC	3 cut(s)	749, 1277, 1548
BstACI	GRCGYC	5 cut(s)	194, 406, 441, 560, 621
BstAFI	CTTAAG	1 cut(s)	1487
BstAPI	GCANNNNNTGC	1 cut(s)	1276
BstAUI	TGTACA	1 cut(s)	912
BstBAI	YACGTR	1 cut(s)	1206
BstBI	TTCGAA	1 cut(s)	1165
BstC8I	GCNNGC	5 cut(s)	236, 395, 980, 1475, 1945
BstDEI	CTNAG	4 cut(s)	1134, 1382, 1467, 1838
BstDSI	CCRYGG	1 cut(s)	523
BstF5I	GGATG	6 cut(s)	202, 363, 420, 483, 1189, 2004
BstFNI	CGCG	5 cut(s)	300, 393, 501, 596, 626
BstH2I	RGCGCY	4 cut(s)	409, 563, 1474, 1495
BstHHI	GCGC	8 cut(s)	302, 408, 503, 546, 562, 628, 1473, 1494
BstKTI	GATC	7 cut(s)	435, 801, 988, 1408, 1906, 1961, 1989
BstMAI	GTCTC	3 cut(s)	382, 1508, 1903
BstMBI	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
BstMCI	CGRYCG	1 cut(s)	1546
BstMWI	GCNNNNNNNGC	7 cut(s)	231, 403, 634, 1043, 1276, 1483, 1586
BstNI	CCWGG	4 cut(s)	257, 674, 748, 838
BstNSI	RCATGY	1 cut(s)	616
BstSCI	CCNGG	6 cut(s)	255, 657, 665, 672, 746, 836
BstSFI	CTRYAG	1 cut(s)	638
BstSLI	GKGCMC	1 cut(s)	980
BstUI	CGCG	5 cut(s)	300, 393, 501, 596, 626
BstV1I	GCAGC	2 cut(s)	624, 1036
BstV2I	GAAGAC	1 cut(s)	457
BstXI	CCANNNNNNTGG	1 cut(s)	1721
BsuI	GTATCC	2 cut(s)	874, 1691
BsuRI	GGCC	3 cut(s)	528, 818, 836
BtgI	CCRYGG	1 cut(s)	523
BtgZI	GCGATG	1 cut(s)	1143
BtrI	CACGTC	1 cut(s)	996
BtsCI	GGATG	6 cut(s)	202, 363, 420, 483, 1189, 2004
BtsI	GCAGTG	1 cut(s)	515
BtsIMutI	CAGTG	1 cut(s)	515
Cac8I	GCNNGC	5 cut(s)	236, 395, 980, 1475, 1945
CciI	TCATGA	3 cut(s)	1480, 1989, 2024
CfoI	GCGC	8 cut(s)	302, 408, 503, 546, 562, 628, 1473, 1494
Cfr10I	RCCGGY	1 cut(s)	1541
Cfr13I	GGNCC	4 cut(s)	259, 565, 655, 670
CpoI	CGGWCCG	1 cut(s)	565
CseI	GACGC	4 cut(s)	202, 256, 629, 907
CsiI	ACCWGGT	1 cut(s)	672
Csp6I	GTAC	3 cut(s)	451, 913, 1207
CspI	CGGWCCG	1 cut(s)	565
CviQI	GTAC	3 cut(s)	451, 913, 1207
DdeI	CTNAG	4 cut(s)	1134, 1382, 1467, 1838
DinI	GGCGCC	2 cut(s)	407, 561
DpnI	GATC	7 cut(s)	434, 800, 987, 1407, 1905, 1960, 1988
DpnII	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
DraIII	CACNNNGTG	1 cut(s)	996
DrdI	GACNNNNNNGTC	1 cut(s)	266
DriI	GACNNNNNGTC	1 cut(s)	661
DseDI	GACNNNNNNGTC	1 cut(s)	266
Eam1104I	CTCTTC	3 cut(s)	749, 1277, 1548
Eam1105I	GACNNNNNGTC	1 cut(s)	661
EarI	CTCTTC	3 cut(s)	749, 1277, 1548
EciI	GGCGGA	1 cut(s)	136
Ecl136II	GAGCTC	2 cut(s)	554, 1389
Eco130I	CCWWGG	2 cut(s)	241, 1508
Eco24I	GRGCYC	3 cut(s)	399, 556, 1391
Eco31I	GGTCTC	2 cut(s)	382, 1903
Eco47I	GGWCC	4 cut(s)	259, 565, 655, 670
Eco47III	AGCGCT	1 cut(s)	1472
Eco53kI	GAGCTC	2 cut(s)	554, 1389
Eco57I	CTGAAG	2 cut(s)	773, 869
EcoICRI	GAGCTC	2 cut(s)	554, 1389
EcoO109I	RGGNCCY	1 cut(s)	670
EcoRII	CCWGG	4 cut(s)	255, 672, 746, 836
EcoT14I	CCWWGG	2 cut(s)	241, 1508
EcoT38I	GRGCYC	3 cut(s)	399, 556, 1391
EgeI	GGCGCC	2 cut(s)	407, 561
EheI	GGCGCC	2 cut(s)	407, 561
ErhI	CCWWGG	2 cut(s)	241, 1508
FalI	AAGNNNNNCTT	1 cut(s)	1999
FaqI	GGGAC	3 cut(s)	184, 334, 683
FauI	CCCGC	4 cut(s)	230, 359, 398, 987
FauNDI	CATATG	1 cut(s)	1741
FbaI	TGATCA	2 cut(s)	798, 1405
FblI	GTMKAC	1 cut(s)	270
Fnu4HI	GCNGC	6 cut(s)	232, 409, 624, 638, 1025, 1362
FokI	GGATG	6 cut(s)	189, 350, 407, 470, 1196, 2011
FriOI	GRGCYC	3 cut(s)	399, 556, 1391
Fsp4HI	GCNGC	6 cut(s)	232, 409, 624, 638, 1025, 1362
FspBI	CTAG	4 cut(s)	5, 705, 1509, 1727
GlaI	GCGC	8 cut(s)	301, 407, 502, 545, 561, 627, 1472, 1493
GluI	GCNGC	6 cut(s)	232, 409, 624, 638, 1025, 1362
GsaI	CCCAGC	2 cut(s)	1347, 1593
GsuI	CTGGAG	2 cut(s)	184, 1557
HaeII	RGCGCY	4 cut(s)	409, 563, 1474, 1495
HaeIII	GGCC	3 cut(s)	528, 818, 836
HapII	CCGG	9 cut(s)	563, 609, 658, 666, 773, 878, 1542, 1648, 1984
HgaI	GACGC	4 cut(s)	202, 256, 629, 907
HhaI	GCGC	8 cut(s)	302, 408, 503, 546, 562, 628, 1473, 1494
Hin1I	GRCGYC	5 cut(s)	194, 406, 441, 560, 621
Hin6I	GCGC	8 cut(s)	300, 406, 501, 544, 560, 626, 1471, 1492
HinP1I	GCGC	8 cut(s)	300, 406, 501, 544, 560, 626, 1471, 1492
HincII	GTYRAC	1 cut(s)	271
HindII	GTYRAC	1 cut(s)	271
HinfI	GANTC	3 cut(s)	123, 1291, 1976
HpaII	CCGG	9 cut(s)	563, 609, 658, 666, 773, 878, 1542, 1648, 1984
HphI	GGTGA	6 cut(s)	85, 91, 97, 277, 583, 1609
Hpy166II	GTNNAC	1 cut(s)	271
Hpy188I	TCNGA	4 cut(s)	958, 1422, 1816, 1903
Hpy188III	TCNNGA	6 cut(s)	163, 991, 1481, 1641, 1990, 2025
Hpy8I	GTNNAC	1 cut(s)	271
HpyCH4III	ACNGT	3 cut(s)	714, 886, 1873
HpyCH4IV	ACGT	5 cut(s)	441, 477, 492, 995, 1205
HpyF10VI	GCNNNNNNNGC	7 cut(s)	231, 403, 634, 1043, 1276, 1483, 1586
HpyF3I	CTNAG	4 cut(s)	1134, 1382, 1467, 1838
HpySE526I	ACGT	5 cut(s)	441, 477, 492, 995, 1205
Hsp92I	GRCGYC	5 cut(s)	194, 406, 441, 560, 621
HspAI	GCGC	8 cut(s)	300, 406, 501, 544, 560, 626, 1471, 1492
KasI	GGCGCC	2 cut(s)	405, 559
Ksp22I	TGATCA	2 cut(s)	798, 1405
Kzo9I	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
LmnI	GCTCC	7 cut(s)	134, 243, 416, 1062, 1189, 1386, 1538
Lsp1109I	GCAGC	2 cut(s)	624, 1036
LweI	GCATC	6 cut(s)	1120, 1161, 1762, 1779, 1939, 1956
MabI	ACCWGGT	1 cut(s)	672
MaeI	CTAG	4 cut(s)	5, 705, 1509, 1727
MaeII	ACGT	5 cut(s)	441, 477, 492, 995, 1205
MaeIII	GTNAC	3 cut(s)	1100, 1201, 1749
MalI	GATC	7 cut(s)	434, 800, 987, 1407, 1905, 1960, 1988
MbiI	CCGCTC	2 cut(s)	129, 411
MboI	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
MhlI	GDGCHC	5 cut(s)	318, 399, 556, 980, 1391
MluCI	AATT	5 cut(s)	73, 732, 1349, 1521, 1660
Mly113I	GGCGCC	2 cut(s)	406, 560
MlyI	GAGTC	1 cut(s)	117
MmeI	TCCRAC	1 cut(s)	44
MroXI	GAANNNNTTC	1 cut(s)	1152
MseI	TTAA	3 cut(s)	1488, 1866, 1971
MslI	CAYNNNNRTG	1 cut(s)	1670
MspA1I	CMGCKG	1 cut(s)	1027
MspCI	CTTAAG	1 cut(s)	1487
MspI	CCGG	9 cut(s)	563, 609, 658, 666, 773, 878, 1542, 1648, 1984
MspR9I	CCNGG	6 cut(s)	257, 659, 667, 674, 748, 838
Mva1269I	GAATGC	1 cut(s)	1306
MvaI	CCWGG	4 cut(s)	257, 674, 748, 838
MvnI	CGCG	5 cut(s)	300, 393, 501, 596, 626
MwoI	GCNNNNNNNGC	7 cut(s)	231, 403, 634, 1043, 1276, 1483, 1586
NarI	GGCGCC	2 cut(s)	406, 560
NciI	CCSGG	2 cut(s)	659, 667
NdeI	CATATG	1 cut(s)	1741
NdeII	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
NlaIV	GGNNCC	5 cut(s)	247, 407, 561, 671, 1540
NmuCI	GTSAC	1 cut(s)	1201
NspI	RCATGY	1 cut(s)	616
NspV	TTCGAA	1 cut(s)	1165
PagI	TCATGA	3 cut(s)	1480, 1989, 2024
PcsI	WCGNNNNNNNCGW	5 cut(s)	276, 423, 474, 531, 1211
PctI	GAATGC	1 cut(s)	1306
PdmI	GAANNNNTTC	1 cut(s)	1152
PfeI	GAWTC	2 cut(s)	1291, 1976
PflFI	GACNNNGTC	1 cut(s)	196
PflMI	CCANNNNNTGG	1 cut(s)	510
PfoI	TCCNGGA	1 cut(s)	255
PkrI	GCNGC	6 cut(s)	233, 410, 625, 639, 1026, 1363
PleI	GAGTC	1 cut(s)	117
PluTI	GGCGCC	2 cut(s)	409, 563
PpsI	GAGTC	1 cut(s)	117
Ppu21I	YACGTR	1 cut(s)	1206
PpuMI	RGGWCCY	1 cut(s)	670
PsiI	TTATAA	1 cut(s)	789
Psp124BI	GAGCTC	2 cut(s)	556, 1391
Psp5II	RGGWCCY	1 cut(s)	670
Psp6I	CCWGG	4 cut(s)	255, 672, 746, 836
PspFI	CCCAGC	2 cut(s)	1343, 1589
PspGI	CCWGG	4 cut(s)	255, 672, 746, 836
PspN4I	GGNNCC	5 cut(s)	247, 407, 561, 671, 1540
PspPI	GGNCC	4 cut(s)	259, 565, 655, 670
PspPPI	RGGWCCY	1 cut(s)	670
PstI	CTGCAG	1 cut(s)	642
PsyI	GACNNNGTC	1 cut(s)	196
PvuII	CAGCTG	1 cut(s)	1027
RsaI	GTAC	3 cut(s)	452, 914, 1208
RsaNI	GTAC	3 cut(s)	451, 913, 1207
RseI	CAYNNNNRTG	1 cut(s)	1670
Rsr2I	CGGWCCG	1 cut(s)	565
RsrII	CGGWCCG	1 cut(s)	565
SacI	GAGCTC	2 cut(s)	556, 1391
SalI	GTCGAC	1 cut(s)	269
SaqAI	TTAA	3 cut(s)	1488, 1866, 1971
SatI	GCNGC	6 cut(s)	232, 409, 624, 638, 1025, 1362
Sau3AI	GATC	7 cut(s)	432, 798, 985, 1405, 1903, 1958, 1986
Sau96I	GGNCC	4 cut(s)	259, 565, 655, 670
SchI	GAGTC	1 cut(s)	117
ScrFI	CCNGG	6 cut(s)	257, 659, 667, 674, 748, 838
SduI	GDGCHC	5 cut(s)	318, 399, 556, 980, 1391
SexAI	ACCWGGT	1 cut(s)	672
SfaNI	GCATC	6 cut(s)	1120, 1161, 1762, 1779, 1939, 1956
SfcI	CTRYAG	1 cut(s)	638
SfoI	GGCGCC	2 cut(s)	407, 561
SfuI	TTCGAA	1 cut(s)	1165
SinI	GGWCC	4 cut(s)	259, 565, 655, 670
SmiMI	CAYNNNNRTG	1 cut(s)	1670
SmlI	CTYRAG	4 cut(s)	1082, 1241, 1487, 1639
SmoI	CTYRAG	4 cut(s)	1082, 1241, 1487, 1639
Sse9I	AATT	5 cut(s)	73, 732, 1349, 1521, 1660
SspDI	GGCGCC	2 cut(s)	405, 559
SspI	AATATT	1 cut(s)	1003
SspMI	CTAG	4 cut(s)	5, 705, 1509, 1727
SstI	GAGCTC	2 cut(s)	556, 1391
StyD4I	CCNGG	6 cut(s)	255, 657, 665, 672, 746, 836
StyI	CCWWGG	2 cut(s)	241, 1508
TaaI	ACNGT	3 cut(s)	714, 886, 1873
TaiI	ACGT	5 cut(s)	444, 480, 495, 998, 1208
TasI	AATT	5 cut(s)	73, 732, 1349, 1521, 1660
TatI	WGTACW	2 cut(s)	450, 912
TauI	GCSGC	4 cut(s)	234, 411, 626, 1364
TfiI	GAWTC	2 cut(s)	1291, 1976
Tru1I	TTAA	3 cut(s)	1488, 1866, 1971
Tru9I	TTAA	3 cut(s)	1488, 1866, 1971
TscAI	CASTG	1 cut(s)	515
TseFI	GTSAC	1 cut(s)	1201
TseI	GCWGC	2 cut(s)	637, 1024
Tsp45I	GTSAC	1 cut(s)	1201
TspGWI	ACGGA	2 cut(s)	451, 1562
TspRI	CASTG	1 cut(s)	515
Tth111I	GACNNNGTC	1 cut(s)	196
Van91I	CCANNNNNTGG	1 cut(s)	510
Vha464I	CTTAAG	1 cut(s)	1487
VpaK11BI	GGWCC	4 cut(s)	259, 565, 655, 670
XceI	RCATGY	1 cut(s)	616
XcmI	CCANNNNNNNNNTGG	1 cut(s)	370
XmaJI	CCTAGG	1 cut(s)	1508
XmiI	GTMKAC	1 cut(s)	270
XmnI	GAANNNNTTC	1 cut(s)	1152
XspI	CTAG	4 cut(s)	5, 705, 1509, 1727
ZraI	GACGTC	1 cut(s)	442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.