Rmu_sc0006915.1_g000006

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006915.1
OG0010879
Physical Location & Seq
Reverse (-)
16829 .. 18894
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
atggataagcagctccggcgagtgagtcctttggttggttcagaggccgcctcgggaatcgtgtttccgggaaagcttcacgtgtttgatttgtcttgttcgttggacattggaagtggagtagttgaaattcatggcacaatcgccgttgtccttcagactatatattgggtcgggttaaatgactgtcattttatccagaagcagaagaacccacacccgctcgcagaaatggatcgagtaatgcaggcaccgtacgatgctaccgttcgattcgcgctggcgtcactggagaggaatctgttacctgacgccgttataaggcggttcacgcgcttgctcttagcgagtcggctgcgctccggttaccagccttcctctgagctccagctctcccaactcctcctgttcgtaaagtctctacaagagatgccaatagctatcaagactgatgacgctaaagctcaacactatgaagtacccacttctttcttcaagacggtgctcgggaacaatctcaaatatagctgctgttacttcaccgataagtcaagcagcttggaggatgcggagagagccatgttggagatgtactgtgaaagatcgcagattaaagatggccatactgttcttgatgttggatgtggctggggatcactgtctctgtacattgcacagaaatataagagctgcagggttactgggatctgcaattcagcaacacagaaggcttttatagaagagcagtgtagggatcgtcggctggagaatgtggagatcattgttgcagatatcagcacatttgagatggaggcatcatatgacagaatattttccatcgaaatgttcgagcacatgaagaactacagggatcttctgaagaagatatccgggtggatgaaacaggacggccttctgtttgtacatcatttctgtcacaaagcatttgcttaccactttgaggacaaaagtgatgatgactggattactaggtatttcttcagtggaggtacaatgccttcagcaaatctactactgtatttccaggatgatgtttctgttgtcgatcattggcttgtcaatgggaagcattatgcacaaacaagtgaagaatggctgaaaagaatggaccataattcggcttccataaaaccaattatggagtcaacttatggcaaggattcggctgtgaagtggactgtgtattggagaactttctttattgcggttgcagaactctttggctacaacaacggagaagaatggatggttgctctcttcctattcaaaaagaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

432

Amino Acids

49.66

Weight (kDa)

6.4

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 152 - 389 2.1e-45 Mycolic acid cyclopropane synthetase
TRM5-TYW2_MTfase PF02475 202 - 315 4.6e-06 TRM5/TYW2 methyltransferase domain
Methyltransf_31 PF13847 204 - 325 6.6e-14 Methyltransferase domain
Methyltransf_23 PF13489 204 - 312 1.4e-11 Methyltransferase domain
TehB PF03848 205 - 288 4.6e-06 Tellurite resistance protein TehB
MTS PF05175 206 - 279 7.1e-07 Methyltransferase small domain
Methyltransf_25 PF13649 210 - 304 1.5e-15 Methyltransferase domain
Methyltransf_11 PF08241 211 - 308 9.1e-15 Methyltransferase domain
Methyltransf_12 PF08242 211 - 306 7.1e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 250
AccBSI CCGCTC 1 cut(s) 223
AccII CGCG 2 cut(s) 278, 334
AciI CCGC 5 cut(s) 48, 221, 325, 569, 1226
AclWI GGATC 5 cut(s) 243, 661, 713, 762, 879
AcoI YGGCCR 1 cut(s) 619
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 4 cut(s) 140, 899, 985, 1005
AcvI CACGTG 1 cut(s) 82
AcyI GRCGYC 2 cut(s) 284, 312
AfaI GTAC 6 cut(s) 257, 480, 593, 668, 924, 1012
AfiI CCNNNNNNNGG 4 cut(s) 35, 321, 961, 1138
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 3 cut(s) 128, 496, 1288
AjnI CCWGG 1 cut(s) 1044
AluBI AGCT 9 cut(s) 13, 76, 385, 391, 440, 464, 528, 558, 690
AluI AGCT 9 cut(s) 13, 76, 385, 391, 440, 464, 528, 558, 690
Alw21I GWGCWC 3 cut(s) 387, 507, 855
Alw26I GTCTC 2 cut(s) 423, 666
AlwI GGATC 5 cut(s) 243, 661, 713, 762, 879
Ama87I CYCGRG 2 cut(s) 52, 506
AoxI GGCC 3 cut(s) 45, 619, 910
ApeKI GCWGC 5 cut(s) 10, 355, 528, 555, 690
ApoI RAATTY 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 832
AspLEI GCGC 3 cut(s) 280, 336, 360
AspS9I GGNCC 1 cut(s) 1129
AsuC2I CCSGG 2 cut(s) 69, 892
AsuHPI GGTGA 1 cut(s) 532
AvaI CYCGRG 2 cut(s) 52, 506
AvaII GGWCC 1 cut(s) 1129
BalI TGGCCA 1 cut(s) 621
BanI GGYRCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 1003, 1035
BbrPI CACGTG 1 cut(s) 82
Bbv12I GWGCWC 3 cut(s) 387, 507, 855
BbvI GCAGC 5 cut(s) 22, 342, 515, 567, 677
BccI CCATC 4 cut(s) 611, 802, 845, 1261
BceAI ACGGC 3 cut(s) 131, 299, 925
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 1 cut(s) 1046
BcnI CCSGG 2 cut(s) 69, 892
BcoDI GTCTC 2 cut(s) 423, 666
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 2 cut(s) 691, 865
BisI GCNGC 6 cut(s) 11, 48, 356, 529, 556, 691
BlsI GCNGC 6 cut(s) 12, 49, 357, 530, 557, 692
Bme1390I CCNGG 3 cut(s) 69, 892, 1046
Bme18I GGWCC 1 cut(s) 1129
BmeT110I CYCGRG 2 cut(s) 52, 506
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 3 cut(s) 69, 892, 1046
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 4 cut(s) 250, 420, 556, 824
BmuI ACTGGG 1 cut(s) 711
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 3 cut(s) 311, 371, 785
BpuMI CCSGG 2 cut(s) 69, 892
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 2 cut(s) 284, 312
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 362
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 321, 961, 1138
Bse1I ACTGG 3 cut(s) 294, 706, 986
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 1046
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 5 cut(s) 571, 647, 903, 1054, 1272
BseLI CCNNNNNNNGG 4 cut(s) 35, 321, 961, 1138
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 3 cut(s) 294, 706, 986
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 5 cut(s) 22, 342, 515, 567, 677
BseYI CCCAGC 1 cut(s) 648
Bsh1236I CGCG 2 cut(s) 278, 334
BshFI GGCC 3 cut(s) 47, 621, 912
BshNI GGYRCC 1 cut(s) 250
BsiHKAI GWGCWC 3 cut(s) 387, 507, 855
BsiHKCI CYCGRG 2 cut(s) 52, 506
BsiSI CCGG 4 cut(s) 16, 68, 363, 891
BsiWI CGTACG 1 cut(s) 255
BslI CCNNNNNNNGG 4 cut(s) 35, 321, 961, 1138
BsmAI GTCTC 2 cut(s) 423, 666
BsnI GGCC 3 cut(s) 47, 621, 912
BsoBI CYCGRG 2 cut(s) 52, 506
Bsp1286I GDGCHC 3 cut(s) 387, 507, 855
Bsp1407I TGTACA 2 cut(s) 666, 922
Bsp143I GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
BspACI CCGC 5 cut(s) 48, 221, 325, 569, 1226
BspANI GGCC 3 cut(s) 47, 621, 912
BspCNI CTCAG 1 cut(s) 373
BspFNI CGCG 2 cut(s) 278, 334
BspLI GGNNCC 1 cut(s) 252
BspMAI CTGCAG 1 cut(s) 695
BspPI GGATC 5 cut(s) 243, 661, 713, 762, 879
BspQI GCTCTTC 1 cut(s) 735
BspT107I GGYRCC 1 cut(s) 250
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 669
BsrGI TGTACA 2 cut(s) 666, 922
BsrI ACTGG 3 cut(s) 294, 706, 986
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
BssNI GRCGYC 2 cut(s) 284, 312
Bst2UI CCWGG 1 cut(s) 1046
Bst4CI ACNGT 9 cut(s) 188, 255, 268, 502, 596, 628, 660, 1038, 1201
Bst6I CTCTTC 2 cut(s) 735, 1283
BstACI GRCGYC 2 cut(s) 284, 312
BstAUI TGTACA 2 cut(s) 666, 922
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 4 cut(s) 225, 249, 282, 338
BstDEI CTNAG 2 cut(s) 343, 381
BstEII GGTNACC 1 cut(s) 365
BstF5I GGATG 5 cut(s) 571, 647, 903, 1054, 1272
BstFNI CGCG 2 cut(s) 278, 334
BstHHI GCGC 3 cut(s) 280, 336, 360
BstKTI GATC 8 cut(s) 238, 605, 656, 708, 757, 780, 874, 1069
BstMAI GTCTC 2 cut(s) 423, 666
BstMBI GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
BstMWI GCNNNNNNNGC 3 cut(s) 16, 331, 575
BstNI CCWGG 1 cut(s) 1046
BstPI GGTNACC 1 cut(s) 365
BstSCI CCNGG 3 cut(s) 67, 890, 1044
BstSFI CTRYAG 2 cut(s) 691, 865
BstUI CGCG 2 cut(s) 278, 334
BstV1I GCAGC 5 cut(s) 22, 342, 515, 567, 677
BstX2I RGATCY 2 cut(s) 705, 871
BstYI RGATCY 2 cut(s) 705, 871
BsuRI GGCC 3 cut(s) 47, 621, 912
BtsCI GGATG 5 cut(s) 571, 647, 903, 1054, 1272
BtsI GCAGTG 1 cut(s) 752
BtsIMutI CAGTG 4 cut(s) 287, 656, 752, 1009
Cac8I GCNNGC 4 cut(s) 225, 249, 282, 338
CfoI GCGC 3 cut(s) 280, 336, 360
Cfr13I GGNCC 1 cut(s) 1129
CseI GACGC 3 cut(s) 273, 320, 464
Csp6I GTAC 6 cut(s) 256, 479, 592, 667, 923, 1011
CviAII CATG 3 cut(s) 134, 580, 856
CviQI GTAC 6 cut(s) 256, 479, 592, 667, 923, 1011
DdeI CTNAG 2 cut(s) 343, 381
DpnI GATC 8 cut(s) 237, 604, 655, 707, 756, 779, 873, 1068
DpnII GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
EaeI YGGCCR 1 cut(s) 619
Eam1104I CTCTTC 2 cut(s) 735, 1283
EarI CTCTTC 2 cut(s) 735, 1283
Ecl136II GAGCTC 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 387
Eco32I GATATC 2 cut(s) 793, 888
Eco47I GGWCC 1 cut(s) 1129
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 4 cut(s) 140, 899, 985, 1005
Eco72I CACGTG 1 cut(s) 82
Eco88I CYCGRG 2 cut(s) 52, 506
Eco91I GGTNACC 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 385
EcoO65I GGTNACC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 1044
EcoRV GATATC 2 cut(s) 793, 888
EcoT38I GRGCYC 1 cut(s) 387
FaeI CATG 3 cut(s) 137, 583, 859
FatI CATG 3 cut(s) 133, 579, 855
FauI CCCGC 1 cut(s) 228
FauNDI CATATG 1 cut(s) 820
Fnu4HI GCNGC 6 cut(s) 11, 48, 356, 529, 556, 691
FokI GGATG 5 cut(s) 578, 654, 910, 1061, 1279
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 6 cut(s) 11, 48, 356, 529, 556, 691
FspBI CTAG 1 cut(s) 990
GlaI GCGC 3 cut(s) 279, 335, 359
GluI GCNGC 6 cut(s) 11, 48, 356, 529, 556, 691
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 3 cut(s) 311, 371, 785
HaeIII GGCC 3 cut(s) 47, 621, 912
HapII CCGG 4 cut(s) 16, 68, 363, 891
HgaI GACGC 3 cut(s) 273, 320, 464
HhaI GCGC 3 cut(s) 280, 336, 360
Hin1I GRCGYC 2 cut(s) 284, 312
Hin1II CATG 3 cut(s) 137, 583, 859
Hin6I GCGC 3 cut(s) 278, 334, 358
HinP1I GCGC 3 cut(s) 278, 334, 358
HincII GTYRAC 1 cut(s) 1167
HindII GTYRAC 1 cut(s) 1167
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 7 cut(s) 25, 57, 273, 298, 349, 1163, 1181
HpaII CCGG 4 cut(s) 16, 68, 363, 891
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 3 cut(s) 330, 1167, 1197
Hpy188I TCNGA 4 cut(s) 43, 159, 382, 879
Hpy188III TCNNGA 6 cut(s) 54, 199, 445, 496, 508, 632
Hpy8I GTNNAC 3 cut(s) 330, 1167, 1197
Hpy99I CGWCG 1 cut(s) 762
HpyAV CCTTC 5 cut(s) 164, 384, 721, 923, 1029
HpyCH4III ACNGT 9 cut(s) 188, 255, 268, 502, 596, 628, 660, 1038, 1201
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 7 cut(s) 247, 674, 693, 711, 788, 1097, 1232
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 331, 575
HpyF3I CTNAG 2 cut(s) 343, 381
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 2 cut(s) 284, 312
Hsp92II CATG 3 cut(s) 137, 583, 859
HspAI GCGC 3 cut(s) 278, 334, 358
Kzo9I GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 3 cut(s) 18, 365, 390
Lsp1109I GCAGC 5 cut(s) 22, 342, 515, 567, 677
LweI GCATC 4 cut(s) 250, 420, 556, 824
MaeI CTAG 1 cut(s) 990
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 6 cut(s) 285, 303, 365, 533, 697, 935
MalI GATC 8 cut(s) 237, 604, 655, 707, 756, 779, 873, 1068
MbiI CCGCTC 1 cut(s) 223
MboI GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
MflI RGATCY 2 cut(s) 705, 871
MhlI GDGCHC 3 cut(s) 387, 507, 855
MlsI TGGCCA 1 cut(s) 621
MluCI AATT 4 cut(s) 129, 712, 1135, 1155
MluNI TGGCCA 1 cut(s) 621
MlyI GAGTC 3 cut(s) 34, 358, 1172
MmeI TCCRAC 3 cut(s) 84, 564, 619
MnlI CCTC 9 cut(s) 37, 61, 288, 388, 413, 556, 805, 955, 1001
Mox20I TGGCCA 1 cut(s) 621
MroXI GAANNNNTTC 1 cut(s) 832
MscI TGGCCA 1 cut(s) 621
MseI TTAA 2 cut(s) 179, 612
MslI CAYNNNNRTG 1 cut(s) 842
Msp20I TGGCCA 1 cut(s) 621
MspI CCGG 4 cut(s) 16, 68, 363, 891
MspR9I CCNGG 3 cut(s) 69, 892, 1046
MvaI CCWGG 1 cut(s) 1046
MvnI CGCG 2 cut(s) 278, 334
MwoI GCNNNNNNNGC 3 cut(s) 16, 331, 575
NciI CCSGG 2 cut(s) 69, 892
NdeI CATATG 1 cut(s) 820
NdeII GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
NlaIII CATG 3 cut(s) 137, 583, 859
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 2 cut(s) 285, 935
PciSI GCTCTTC 1 cut(s) 735
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 846
PdmI GAANNNNTTC 1 cut(s) 832
PfeI GAWTC 4 cut(s) 57, 273, 298, 1181
Pfl23II CGTACG 1 cut(s) 255
PfoI TCCNGGA 2 cut(s) 67, 1044
PkrI GCNGC 6 cut(s) 12, 49, 357, 530, 557, 692
PleI GAGTC 3 cut(s) 33, 357, 1171
PmaCI CACGTG 1 cut(s) 82
PmlI CACGTG 1 cut(s) 82
PpsI GAGTC 3 cut(s) 33, 357, 1171
Ppu21I YACGTR 1 cut(s) 82
PsiI TTATAA 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 1044
PspCI CACGTG 1 cut(s) 82
PspEI GGTNACC 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 1 cut(s) 1044
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 1 cut(s) 1129
PstI CTGCAG 1 cut(s) 695
PsuI RGATCY 2 cut(s) 705, 871
RsaI GTAC 6 cut(s) 257, 480, 593, 668, 924, 1012
RsaNI GTAC 6 cut(s) 256, 479, 592, 667, 923, 1011
RseI CAYNNNNRTG 1 cut(s) 842
SacI GAGCTC 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 2 cut(s) 179, 612
SatI GCNGC 6 cut(s) 11, 48, 356, 529, 556, 691
Sau3AI GATC 8 cut(s) 235, 602, 653, 705, 754, 777, 871, 1066
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 3 cut(s) 34, 358, 1172
ScrFI CCNGG 3 cut(s) 69, 892, 1046
SduI GDGCHC 3 cut(s) 387, 507, 855
SfaNI GCATC 4 cut(s) 250, 420, 556, 824
SfcI CTRYAG 2 cut(s) 691, 865
SinI GGWCC 1 cut(s) 1129
SmiMI CAYNNNNRTG 1 cut(s) 842
Sse9I AATT 4 cut(s) 129, 712, 1135, 1155
SsiI CCGC 5 cut(s) 48, 221, 325, 569, 1226
SspI AATATT 1 cut(s) 831
SspMI CTAG 1 cut(s) 990
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 3 cut(s) 67, 890, 1044
TaaI ACNGT 9 cut(s) 188, 255, 268, 502, 596, 628, 660, 1038, 1201
TaiI ACGT 1 cut(s) 84
TaqI TCGA 5 cut(s) 238, 271, 840, 849, 1065
TasI AATT 4 cut(s) 129, 712, 1135, 1155
TatI WGTACW 3 cut(s) 591, 666, 922
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 4 cut(s) 57, 273, 298, 1181
Tru1I TTAA 2 cut(s) 179, 612
Tru9I TTAA 2 cut(s) 179, 612
TscAI CASTG 4 cut(s) 294, 663, 752, 1009
TseFI GTSAC 2 cut(s) 285, 935
TseI GCWGC 5 cut(s) 10, 355, 528, 555, 690
Tsp45I GTSAC 2 cut(s) 285, 935
TspDTI ATGAA 4 cut(s) 122, 489, 872, 914
TspGWI ACGGA 1 cut(s) 1269
TspRI CASTG 4 cut(s) 294, 663, 752, 1009
VpaK11BI GGWCC 1 cut(s) 1129
XapI RAATTY 1 cut(s) 129
XmnI GAANNNNTTC 1 cut(s) 832
XspI CTAG 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.