Rmu_sc0006954.1_g000007

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006954.1
OG0004218
Physical Location & Seq
Forward (+)
40150 .. 46870
6721 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1719 bp
atgcgatccaacatgatcatcatttacgttagattctggctgtggcatgcttttattctactgctgcttacttccaactttgctcttgcacagtcgatcatcaagaacttaccagccttccagggtgacctccctttcaagcttgaaactgggtatgtgggcgtgggagattcagaagaggtgcaactgttctactactttgttgagtcagaaaggagtccagaaaatgatcctctgctgatttggttgacaggaggtcctcgatgctcttcgttttatggcctcatctacgaaattggtccattatctttcaaacttacaaatttgactacagatcccttaacacttgttctgaatccaaactcgtggaacaaggtagcaaacatcatattcttagatgctcctgctggtactggttactcctactccaccacttcccacgggttaatctctagtgacaccttacattcaacccgtatttgtgagtttctccagaagtggcttacgactcatcaaaagttccttgcaaatccactctatgttgctggtgactcttactctggcaaaatcgttcccatcattgcccagaagatagctgatggtattgaagctggaatcaagccacatttgaatctccagggatacatagttggtaatccagtttcaaatgcacaacaggacgttaattccagaattgtatatgctcatcatctgggacttatatcagatagaatatatcagtcagctaacaggaattgcaagggcgaatttgtagattttgatccaaacaatcaactgtgcctgaacagccttcaagcaatcgatgagtgcattgggagaattgataaagaacatatcttggcaccattttgccttgcatcaattaaccataaccaaaattccatactggaaatgaactgggagtctaatgatgacagtttcttggacttttcatttcctatggtacaaatgtgtagggaagataagattcggtattccgttctatggcaaaatgatataaatgttcgcaaagctcttggtattcgggagggaacaaaagggcaatggactaaatgcaataaaaccactccctatacatttgacatcgctagtaatattgcgtatcatcgcaatctcagccgaaaatcccttcgtgctataatctacagcggtgatcatgacatggctgttccatatataagtacactagcatggatagaatccatgaactttacaattgaagaggattggagaccatggttctctaatgatcaagttgcaggatacactctttactattcgaataataaggagtataatttgacctatgcgacaataaagggaggaggacatacagctccagaatacaatcccaaggaatgttttgatatgatcaaaaggcccgaaagtccagtcggcccagatgacaaagccgccgttgcaggggaaggtggtggagagctcggcggtgatgagaacctcggggacaccccacatgaaagggaagatgaggaagtcgagaatggcgagaatggggccggaggcttgaccagacggctcttcgccgtaagggctgccggtgaatttgaagtacatgaggaaagtcagagggataagagtgagcttctttgggtttggagtggtgggcgtggtggttgtggtggtggttattggaggtctggaagtggttttggaggagattccatattgggtttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

572

Amino Acids

64.03

Weight (kDa)

5.14

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 38 - 467 1.4e-120 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 862
AciI CCGC 3 cut(s) 1170, 1434, 1467
AclWI GGATC 3 cut(s) 224, 329, 776
AcsI RAATTY 4 cut(s) 322, 767, 898, 1583
AfaI GTAC 4 cut(s) 412, 966, 1204, 1593
AfiI CCNNNNNNNGG 2 cut(s) 1005, 1443
AhdI GACNNNNNGTC 1 cut(s) 255
AjnI CCWGG 2 cut(s) 120, 636
AluBI AGCT 8 cut(s) 142, 596, 611, 746, 1034, 1358, 1462, 1624
AluI AGCT 8 cut(s) 142, 596, 611, 746, 1034, 1358, 1462, 1624
Alw21I GWGCWC 1 cut(s) 1464
Alw26I GTCTC 1 cut(s) 1246
AlwI GGATC 3 cut(s) 224, 329, 776
Ama87I CYCGRG 1 cut(s) 1481
AoxI GGCC 4 cut(s) 280, 1400, 1417, 1536
ApeKI GCWGC 2 cut(s) 64, 1574
ApoI RAATTY 4 cut(s) 322, 767, 898, 1583
ArsI GACNNNNNNTTYG 2 cut(s) 1398, 1430
AspS9I GGNCC 5 cut(s) 257, 299, 1401, 1418, 1536
AsuHPI GGTGA 5 cut(s) 137, 560, 1184, 1481, 1592
AsuII TTCGAA 1 cut(s) 1301
AvaI CYCGRG 1 cut(s) 1481
AvaII GGWCC 2 cut(s) 257, 299
BaeI ACNNNNGTAYC 2 cut(s) 956, 989
BanI GGYRCC 1 cut(s) 862
BanII GRGCYC 1 cut(s) 1464
BauI CACGAG 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 1464
BbvI GCAGC 2 cut(s) 51, 1561
BccI CCATC 2 cut(s) 584, 593
BceAI ACGGC 3 cut(s) 1421, 1550, 1571
BciT130I CCWGG 2 cut(s) 122, 638
BciVI GTATCC 2 cut(s) 635, 1277
BclI TGATCA 4 cut(s) 15, 1174, 1270, 1392
BcoDI GTCTC 1 cut(s) 1246
BfaI CTAG 3 cut(s) 453, 1110, 1208
BfmI CTRYAG 2 cut(s) 330, 1165
BfuI GTATCC 2 cut(s) 635, 1277
BglI GCCNNNNNGGC 1 cut(s) 1571
BisI GCNGC 3 cut(s) 65, 1434, 1575
BlsI GCNGC 3 cut(s) 66, 1435, 1576
Bme1390I CCNGG 2 cut(s) 122, 638
Bme18I GGWCC 2 cut(s) 257, 299
BmeRI GACNNNNNGTC 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 1481
BmgT120I GGNCC 5 cut(s) 257, 299, 1401, 1418, 1536
BmiI GGNNCC 2 cut(s) 864, 1537
BmrFI CCNGG 2 cut(s) 122, 638
BmrI ACTGGG 2 cut(s) 159, 928
BmsI GCATC 3 cut(s) 254, 388, 887
BmuI ACTGGG 2 cut(s) 159, 928
BpmI CTGGAG 3 cut(s) 476, 620, 1344
Bpu14I TTCGAA 1 cut(s) 1301
Bsa29I ATCGAT 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 1246
BsaJI CCNNGG 6 cut(s) 121, 439, 637, 1256, 1374, 1480
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 2 cut(s) 1005, 1443
Bse118I RCCGGY 1 cut(s) 1577
Bse1I ACTGG 6 cut(s) 154, 418, 659, 912, 923, 1412
Bse3DI GCAATG 2 cut(s) 579, 1070
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 2 cut(s) 122, 638
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 6 cut(s) 121, 439, 637, 1256, 1374, 1480
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 1005, 1443
BseMI GCAATG 2 cut(s) 579, 1070
BseMII CTCAG 1 cut(s) 1150
BseNI ACTGG 6 cut(s) 154, 418, 659, 912, 923, 1412
BseRI GAGGAG 2 cut(s) 1359, 1710
BseXI GCAGC 2 cut(s) 51, 1561
BshFI GGCC 4 cut(s) 282, 1402, 1419, 1538
BshNI GGYRCC 1 cut(s) 862
BshVI ATCGAT 1 cut(s) 822
BsiHKAI GWGCWC 1 cut(s) 1464
BsiHKCI CYCGRG 1 cut(s) 1481
BsiSI CCGG 2 cut(s) 1539, 1578
BslFI GGGAC 2 cut(s) 729, 1499
BslI CCNNNNNNNGG 2 cut(s) 1005, 1443
BsmAI GTCTC 1 cut(s) 1246
BsmFI GGGAC 2 cut(s) 729, 1499
BsnI GGCC 4 cut(s) 282, 1402, 1419, 1538
Bso31I GGTCTC 1 cut(s) 1246
BsoBI CYCGRG 1 cut(s) 1481
Bsp119I TTCGAA 1 cut(s) 1301
Bsp1286I GDGCHC 1 cut(s) 1464
Bsp143I GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
Bsp19I CCATGG 1 cut(s) 1256
BspACI CCGC 3 cut(s) 1170, 1434, 1467
BspANI GGCC 4 cut(s) 282, 1402, 1419, 1538
BspCNI CTCAG 1 cut(s) 1149
BspDI ATCGAT 1 cut(s) 822
BspHI TCATGA 1 cut(s) 1177
BspLI GGNNCC 2 cut(s) 864, 1537
BspPI GGATC 3 cut(s) 224, 329, 776
BspQI GCTCTTC 2 cut(s) 274, 1565
BspT104I TTCGAA 1 cut(s) 1301
BspT107I GGYRCC 1 cut(s) 862
BspTNI GGTCTC 1 cut(s) 1246
BsrDI GCAATG 2 cut(s) 579, 1070
BsrFI RCCGGY 1 cut(s) 1577
BsrI ACTGG 6 cut(s) 154, 418, 659, 912, 923, 1412
BssAI RCCGGY 1 cut(s) 1577
BssECI CCNNGG 6 cut(s) 121, 439, 637, 1256, 1374, 1480
BssMI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
BssSI CACGAG 1 cut(s) 364
BssT1I CCWWGG 2 cut(s) 1256, 1374
Bst2BI CACGAG 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 122, 638
Bst4CI ACNGT 4 cut(s) 93, 189, 798, 938
Bst6I CTCTTC 4 cut(s) 171, 274, 1236, 1565
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 2 cut(s) 394, 1136
BstDSI CCRYGG 2 cut(s) 439, 1256
BstEII GGTNACC 1 cut(s) 125
BstKTI GATC 9 cut(s) 8, 18, 99, 232, 337, 784, 1177, 1273, 1395
BstMAI GTCTC 1 cut(s) 1246
BstMBI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
BstMWI GCNNNNNNNGC 4 cut(s) 807, 1137, 1439, 1571
BstNI CCWGG 2 cut(s) 122, 638
BstNSI RCATGY 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 120, 636
BstSFI CTRYAG 2 cut(s) 330, 1165
BstV1I GCAGC 2 cut(s) 51, 1561
BstX2I RGATCY 1 cut(s) 334
BstXI CCANNNNNNTGG 1 cut(s) 366
BstYI RGATCY 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 822
BsuI GTATCC 2 cut(s) 635, 1277
BsuRI GGCC 4 cut(s) 282, 1402, 1419, 1538
BsuTUI ATCGAT 1 cut(s) 822
BtgI CCRYGG 2 cut(s) 439, 1256
BtgZI GCGATG 2 cut(s) 1090, 1112
Cac8I GCNNGC 1 cut(s) 48
CciI TCATGA 1 cut(s) 1177
Cfr10I RCCGGY 1 cut(s) 1577
Cfr13I GGNCC 5 cut(s) 257, 299, 1401, 1418, 1536
ClaI ATCGAT 1 cut(s) 822
Csp6I GTAC 4 cut(s) 411, 965, 1203, 1592
CviAII CATG 9 cut(s) 13, 47, 1178, 1183, 1212, 1225, 1257, 1496, 1595
CviQI GTAC 4 cut(s) 411, 965, 1203, 1592
DdeI CTNAG 2 cut(s) 394, 1136
DpnI GATC 9 cut(s) 7, 17, 98, 231, 336, 783, 1176, 1272, 1394
DpnII GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
DriI GACNNNNNGTC 1 cut(s) 255
Eam1104I CTCTTC 4 cut(s) 171, 274, 1236, 1565
Eam1105I GACNNNNNGTC 1 cut(s) 255
EarI CTCTTC 4 cut(s) 171, 274, 1236, 1565
Ecl136II GAGCTC 1 cut(s) 1462
Eco130I CCWWGG 2 cut(s) 1256, 1374
Eco24I GRGCYC 1 cut(s) 1464
Eco31I GGTCTC 1 cut(s) 1246
Eco47I GGWCC 2 cut(s) 257, 299
Eco53kI GAGCTC 1 cut(s) 1462
Eco88I CYCGRG 1 cut(s) 1481
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 1462
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 120, 636
EcoT14I CCWWGG 2 cut(s) 1256, 1374
EcoT38I GRGCYC 1 cut(s) 1464
ErhI CCWWGG 2 cut(s) 1256, 1374
FaeI CATG 9 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260, 1499, 1598
FaqI GGGAC 2 cut(s) 729, 1499
FatI CATG 9 cut(s) 12, 46, 1177, 1182, 1211, 1224, 1256, 1495, 1594
FbaI TGATCA 4 cut(s) 15, 1174, 1270, 1392
Fnu4HI GCNGC 3 cut(s) 65, 1434, 1575
FriOI GRGCYC 1 cut(s) 1464
Fsp4HI GCNGC 3 cut(s) 65, 1434, 1575
FspBI CTAG 3 cut(s) 453, 1110, 1208
GluI GCNGC 3 cut(s) 65, 1434, 1575
GsuI CTGGAG 3 cut(s) 476, 620, 1344
HaeIII GGCC 4 cut(s) 282, 1402, 1419, 1538
HapII CCGG 2 cut(s) 1539, 1578
Hin1II CATG 9 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260, 1499, 1598
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 140
HpaII CCGG 2 cut(s) 1539, 1578
HphI GGTGA 5 cut(s) 137, 560, 1184, 1481, 1592
Hpy166II GTNNAC 2 cut(s) 249, 1205
Hpy188I TCNGA 5 cut(s) 175, 211, 354, 727, 1608
Hpy188III TCNNGA 9 cut(s) 103, 221, 493, 690, 1046, 1178, 1361, 1519, 1680
Hpy8I GTNNAC 2 cut(s) 249, 1205
HpyAV CCTTC 4 cut(s) 127, 821, 1160, 1442
HpyCH4III ACNGT 4 cut(s) 93, 189, 798, 938
HpyCH4IV ACGT 2 cut(s) 27, 681
HpyF10VI GCNNNNNNNGC 4 cut(s) 807, 1137, 1439, 1571
HpyF3I CTNAG 2 cut(s) 394, 1136
HpySE526I ACGT 2 cut(s) 27, 681
Hsp92II CATG 9 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260, 1499, 1598
Ksp22I TGATCA 4 cut(s) 15, 1174, 1270, 1392
Kzo9I GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
LguI GCTCTTC 2 cut(s) 274, 1565
LmnI GCTCC 2 cut(s) 406, 1363
Lsp1109I GCAGC 2 cut(s) 51, 1561
LweI GCATC 3 cut(s) 254, 388, 887
MaeI CTAG 3 cut(s) 453, 1110, 1208
MaeII ACGT 2 cut(s) 27, 681
MaeIII GTNAC 4 cut(s) 125, 416, 455, 548
MalI GATC 9 cut(s) 7, 17, 98, 231, 336, 783, 1176, 1272, 1394
MboI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
MboII GAAGA 7 cut(s) 188, 261, 601, 992, 1253, 1517, 1552
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 1464
MlyI GAGTC 5 cut(s) 215, 226, 502, 545, 932
MmeI TCCRAC 2 cut(s) 33, 99
MseI TTAA 4 cut(s) 341, 446, 684, 885
MslI CAYNNNNRTG 1 cut(s) 1210
MspA1I CMGCKG 1 cut(s) 1170
MspI CCGG 2 cut(s) 1539, 1578
MspR9I CCNGG 2 cut(s) 122, 638
MunI CAATTG 1 cut(s) 1236
MvaI CCWGG 2 cut(s) 122, 638
MwoI GCNNNNNNNGC 4 cut(s) 807, 1137, 1439, 1571
NcoI CCATGG 1 cut(s) 1256
NdeII GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
NlaIII CATG 9 cut(s) 16, 50, 1181, 1186, 1215, 1228, 1260, 1499, 1598
NlaIV GGNNCC 2 cut(s) 864, 1537
NmeAIII GCCGAG 1 cut(s) 1443
NmuCI GTSAC 3 cut(s) 125, 455, 548
NspI RCATGY 1 cut(s) 50
NspV TTCGAA 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 50
PagI TCATGA 1 cut(s) 1177
PciSI GCTCTTC 2 cut(s) 274, 1565
PcsI WCGNNNNNNNCGW 1 cut(s) 1524
PfeI GAWTC 8 cut(s) 33, 170, 355, 615, 631, 988, 1220, 1700
PkrI GCNGC 3 cut(s) 66, 1435, 1576
PleI GAGTC 5 cut(s) 214, 225, 502, 545, 931
PpsI GAGTC 5 cut(s) 214, 225, 502, 545, 931
PpuMI RGGWCCY 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 1464
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 120, 636
PspEI GGTNACC 1 cut(s) 125
PspGI CCWGG 2 cut(s) 120, 636
PspN4I GGNNCC 2 cut(s) 864, 1537
PspPI GGNCC 5 cut(s) 257, 299, 1401, 1418, 1536
PspPPI RGGWCCY 1 cut(s) 257
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 4 cut(s) 412, 966, 1204, 1593
RsaNI GTAC 4 cut(s) 411, 965, 1203, 1592
RseI CAYNNNNRTG 1 cut(s) 1210
SacI GAGCTC 1 cut(s) 1464
SapI GCTCTTC 2 cut(s) 274, 1565
SaqAI TTAA 4 cut(s) 341, 446, 684, 885
SatI GCNGC 3 cut(s) 65, 1434, 1575
Sau3AI GATC 9 cut(s) 5, 15, 96, 229, 334, 781, 1174, 1270, 1392
Sau96I GGNCC 5 cut(s) 257, 299, 1401, 1418, 1536
SchI GAGTC 5 cut(s) 215, 226, 502, 545, 932
ScrFI CCNGG 2 cut(s) 122, 638
SduI GDGCHC 1 cut(s) 1464
SfaNI GCATC 3 cut(s) 254, 388, 887
SfcI CTRYAG 2 cut(s) 330, 1165
SfuI TTCGAA 1 cut(s) 1301
SinI GGWCC 2 cut(s) 257, 299
SmiMI CAYNNNNRTG 1 cut(s) 1210
SphI GCATGC 1 cut(s) 50
SsiI CCGC 3 cut(s) 1170, 1434, 1467
SspI AATATT 1 cut(s) 1117
SspMI CTAG 3 cut(s) 453, 1110, 1208
SstI GAGCTC 1 cut(s) 1464
StyD4I CCNGG 2 cut(s) 120, 636
StyI CCWWGG 2 cut(s) 1256, 1374
TaaI ACNGT 4 cut(s) 93, 189, 798, 938
TaiI ACGT 2 cut(s) 30, 684
TaqI TCGA 5 cut(s) 95, 262, 822, 1301, 1518
TatI WGTACW 2 cut(s) 1202, 1591
TauI GCSGC 1 cut(s) 1436
TfiI GAWTC 8 cut(s) 33, 170, 355, 615, 631, 988, 1220, 1700
Tru1I TTAA 4 cut(s) 341, 446, 684, 885
Tru9I TTAA 4 cut(s) 341, 446, 684, 885
TseFI GTSAC 3 cut(s) 125, 455, 548
TseI GCWGC 2 cut(s) 64, 1574
Tsp45I GTSAC 3 cut(s) 125, 455, 548
TspDTI ATGAA 4 cut(s) 929, 942, 1241, 1512
TspGWI ACGGA 1 cut(s) 988
VpaK11BI GGWCC 2 cut(s) 257, 299
XapI RAATTY 4 cut(s) 322, 767, 898, 1583
XceI RCATGY 1 cut(s) 50
XspI CTAG 3 cut(s) 453, 1110, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.