Rmu_sc0007063.1_g000001
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007063.1
OG0014718
Physical Location & Seq
Forward (+)
675 .. 2372
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
atggatggtagacaagagctgagaaacaacaccaccaccaccaatcgtactactcttcctccttcctcctcctcctcctctgcaccgatggccaactcagcacttttctccgatcacgagattccgatgatacccaacaacaccttcccttcactctcaagcatcttcgactcagaacccgaacggggttatctgggtttcatggagttgctcggcaacacggacttcgctcctgtggttgatttcgggacccaaacgacgccgtctgactcgttggtgacgatgatgatgccacctcagcagcagcagcaaacacaaacacaagcacaagcgcttgcgcttccgtcgccggtgtcgtctacagtgccagagagtgggactaactccgaggccttgaactccggcgccaatactgctcccacgacacccaactcgtcttcgatctcgtcctcgtctaatgaagctcatgcggcgaataataataatgaggagcaaaccacgacaaaagcaggagatgaggaagaagatgctgaccaggatccggacaagacccagaaacaattgaagcccaaaaagaagaaccagaaaaggcagagggagccgagatttgcgttcatgaccaagagcgaggtcgataacctagacgacgggtacagatggcgcaagtacggccaaaaagctgtcaagaacagcccttatccaaggagctactatcgttgcaccacagcaggttgcggtgtgaagaagagggtggagaggtcctccgacgacccttcgattgtggtgacgacttacgaaggccagcacacgcacccgagcccgatcacgccgcgcggcagcatggggatggcgccgctgccgtcggagctgtccggtcatttgttcactggggcaggctcaggctatggtgttgctcatcactatcaacatcagcagcaacagcaaccgcatttgcactcttatatatataactcttcgccttctttgaacattactactaccacctcctcagcgccatcctatggtttcaaccaggcttcgtttcctggtttgtttcaagagagatataataacactggaggaggtcattcctctaatttggccgcttctaatttgcttagagaccacggacttcttcaggatatgctgccatcgcagattcgaaaagagactaaggaagggtag

Protein Analysis

394

Amino Acids

42.99

Weight (kDa)

6.06

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 215 - 272 2.1e-28 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014718)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20230
malus_domestica MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica Prupe.1G114800_v2.0.a1
pyrus_communis pycom13g12950 pycom16g12920
rosa_chinensis RchiOBHm_Chr4g0425801
rosa_laevigata RLG00000007339
rosa_multiflora Rmu_sc0007063.1_g000001
rosa_roxburghii Rroxscaffold_5G00367790
rosa_rugosa Rorug04G0206200 Rorug04G0206300
rosa_samantha Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana Rw4G022740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 719
AccB1I GGYRCC 2 cut(s) 404, 850
AccB7I CCANNNNNTGG 2 cut(s) 374, 1022
AccI GTMKAC 2 cut(s) 10, 359
AccII CGCG 2 cut(s) 832, 834
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 7 cut(s) 470, 735, 830, 834, 854, 947, 1104
AclWI GGATC 2 cut(s) 533, 546
AcoI YGGCCR 3 cut(s) 90, 670, 1101
AcuI CTGAAG 1 cut(s) 1121
AcyI GRCGYC 3 cut(s) 260, 405, 851
AfaI GTAC 3 cut(s) 49, 653, 668
AfeI AGCGCT 1 cut(s) 333
AfiI CCNNNNNNNGG 4 cut(s) 185, 374, 541, 1022
AgsI TTSAA 5 cut(s) 397, 565, 988, 1030, 1058
AjnI CCWGG 3 cut(s) 534, 1032, 1045
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 5 cut(s) 19, 464, 680, 708, 868
AluI AGCT 5 cut(s) 19, 464, 680, 708, 868
Alw26I GTCTC 2 cut(s) 1116, 1163
AlwI GGATC 2 cut(s) 533, 546
Ama87I CYCGRG 1 cut(s) 814
Aor13HI TCCGGA 1 cut(s) 541
Aor51HI AGCGCT 1 cut(s) 333
AoxI GGCC 5 cut(s) 90, 390, 670, 799, 1101
ApeKI GCWGC 7 cut(s) 301, 304, 307, 837, 856, 934, 1147
AspLEI GCGC 7 cut(s) 334, 340, 407, 663, 834, 853, 1015
AspS9I GGNCC 2 cut(s) 249, 759
AsuHPI GGTGA 2 cut(s) 289, 796
AsuII TTCGAA 1 cut(s) 1162
AvaI CYCGRG 1 cut(s) 814
AvaII GGWCC 2 cut(s) 249, 759
BalI TGGCCA 1 cut(s) 92
BamHI GGATCC 1 cut(s) 538
BanI GGYRCC 2 cut(s) 404, 850
BanII GRGCYC 1 cut(s) 821
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 429
BbvCI CCTCAGC 2 cut(s) 297, 1009
BbvI GCAGC 7 cut(s) 313, 316, 319, 843, 849, 946, 1134
BccI CCATC 5 cut(s) 82, 651, 841, 1024, 1159
BceAI ACGGC 3 cut(s) 247, 685, 844
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 3 cut(s) 536, 1034, 1047
BcoDI GTCTC 2 cut(s) 1116, 1163
BfaI CTAG 1 cut(s) 641
BfmI CTRYAG 1 cut(s) 360
BfoI RGCGCY 4 cut(s) 335, 408, 854, 1016
BfuAI ACCTGC 1 cut(s) 719
Bme1390I CCNGG 3 cut(s) 536, 1034, 1047
Bme18I GGWCC 2 cut(s) 249, 759
BmeT110I CYCGRG 1 cut(s) 814
BmgT120I GGNCC 2 cut(s) 249, 759
BmiI GGNNCC 6 cut(s) 250, 251, 406, 540, 600, 852
BmrFI CCNGG 3 cut(s) 536, 1034, 1047
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 3 cut(s) 171, 279, 517
BmuI ACTGGG 1 cut(s) 897
BpiI GAAGAC 1 cut(s) 429
BplI GAGNNNNNCTC 2 cut(s) 746, 778
BpmI CTGGAG 1 cut(s) 1098
Bpu10I CCTNAGC 3 cut(s) 297, 898, 1009
Bpu14I TTCGAA 1 cut(s) 1162
BpuEI CTTGAG 1 cut(s) 142
BsaHI GRCGYC 3 cut(s) 260, 405, 851
BsaI GGTCTC 1 cut(s) 1116
BsaJI CCNNGG 3 cut(s) 387, 701, 1126
BsaWI WCCGGW 2 cut(s) 541, 872
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 374, 541, 1022
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 2 cut(s) 892, 1081
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 3 cut(s) 536, 1034, 1047
BseDI CCNNGG 3 cut(s) 387, 701, 1126
BseGI GGATG 3 cut(s) 10, 852, 1016
BseLI CCNNNNNNNGG 4 cut(s) 185, 374, 541, 1022
BseMII CTCAG 6 cut(s) 11, 111, 186, 311, 912, 1023
BseNI ACTGG 2 cut(s) 892, 1081
BseRI GAGGAG 7 cut(s) 58, 61, 64, 67, 503, 997, 1095
BseXI GCAGC 7 cut(s) 313, 316, 319, 843, 849, 946, 1134
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 832, 834
BshFI GGCC 5 cut(s) 92, 392, 672, 801, 1103
BshNI GGYRCC 2 cut(s) 404, 850
BsiHKCI CYCGRG 1 cut(s) 814
BsiSI CCGG 4 cut(s) 350, 402, 542, 873
BslFI GGGAC 2 cut(s) 262, 391
BslI CCNNNNNNNGG 4 cut(s) 185, 374, 541, 1022
BsmAI GTCTC 2 cut(s) 1116, 1163
BsmFI GGGAC 2 cut(s) 262, 391
BsnI GGCC 5 cut(s) 92, 392, 672, 801, 1103
Bso31I GGTCTC 1 cut(s) 1116
BsoBI CYCGRG 1 cut(s) 814
Bsp119I TTCGAA 1 cut(s) 1162
Bsp1286I GDGCHC 1 cut(s) 821
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 4 cut(s) 112, 441, 538, 822
BspACI CCGC 7 cut(s) 470, 735, 830, 834, 854, 947, 1104
BspANI GGCC 5 cut(s) 92, 392, 672, 801, 1103
BspCNI CTCAG 6 cut(s) 12, 110, 185, 310, 911, 1022
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 2 cut(s) 832, 834
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 6 cut(s) 250, 251, 406, 540, 600, 852
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 2 cut(s) 533, 546
BspT104I TTCGAA 1 cut(s) 1162
BspT107I GGYRCC 2 cut(s) 404, 850
BspTNI GGTCTC 1 cut(s) 1116
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 2 cut(s) 892, 1081
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 3 cut(s) 387, 701, 1126
BssMI GATC 4 cut(s) 112, 441, 538, 822
BssNI GRCGYC 3 cut(s) 260, 405, 851
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 701
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 3 cut(s) 536, 1034, 1047
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 3 cut(s) 60, 740, 979
BstACI GRCGYC 3 cut(s) 260, 405, 851
BstBI TTCGAA 1 cut(s) 1162
BstC8I GCNNGC 3 cut(s) 336, 803, 895
BstDEI CTNAG 8 cut(s) 20, 97, 172, 297, 898, 1009, 1118, 1173
BstDSI CCRYGG 1 cut(s) 1126
BstF5I GGATG 3 cut(s) 10, 852, 1016
BstFNI CGCG 2 cut(s) 832, 834
BstH2I RGCGCY 4 cut(s) 335, 408, 854, 1016
BstHHI GCGC 7 cut(s) 334, 340, 407, 663, 834, 853, 1015
BstKTI GATC 4 cut(s) 115, 444, 541, 825
BstMAI GTCTC 2 cut(s) 1116, 1163
BstMBI GATC 4 cut(s) 112, 441, 538, 822
BstNI CCWGG 3 cut(s) 536, 1034, 1047
BstSCI CCNGG 3 cut(s) 534, 1032, 1045
BstSFI CTRYAG 1 cut(s) 360
BstUI CGCG 2 cut(s) 832, 834
BstV1I GCAGC 7 cut(s) 313, 316, 319, 843, 849, 946, 1134
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BsuRI GGCC 5 cut(s) 92, 392, 672, 801, 1103
BtgI CCRYGG 1 cut(s) 1126
BtgZI GCGATG 1 cut(s) 1137
BtsCI GGATG 3 cut(s) 10, 852, 1016
BtsIMutI CAGTG 3 cut(s) 369, 885, 1074
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 3 cut(s) 336, 803, 895
CciI TCATGA 1 cut(s) 615
CfoI GCGC 7 cut(s) 334, 340, 407, 663, 834, 853, 1015
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 249, 759
CseI GACGC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 48, 652, 667
CviAII CATG 4 cut(s) 202, 467, 616, 841
CviQI GTAC 3 cut(s) 48, 652, 667
DdeI CTNAG 8 cut(s) 20, 97, 172, 297, 898, 1009, 1118, 1173
DinI GGCGCC 2 cut(s) 406, 852
DpnI GATC 4 cut(s) 114, 443, 540, 824
DpnII GATC 4 cut(s) 112, 441, 538, 822
EaeI YGGCCR 3 cut(s) 90, 670, 1101
Eam1104I CTCTTC 3 cut(s) 60, 740, 979
EarI CTCTTC 3 cut(s) 60, 740, 979
Eco130I CCWWGG 1 cut(s) 701
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 1 cut(s) 821
Eco31I GGTCTC 1 cut(s) 1116
Eco47I GGWCC 2 cut(s) 249, 759
Eco47III AGCGCT 1 cut(s) 333
Eco57I CTGAAG 1 cut(s) 1121
Eco88I CYCGRG 1 cut(s) 814
EcoO109I RGGNCCY 2 cut(s) 249, 759
EcoRII CCWGG 3 cut(s) 534, 1032, 1045
EcoT14I CCWWGG 1 cut(s) 701
EcoT38I GRGCYC 1 cut(s) 821
EgeI GGCGCC 2 cut(s) 406, 852
EheI GGCGCC 2 cut(s) 406, 852
ErhI CCWWGG 1 cut(s) 701
FaeI CATG 4 cut(s) 205, 470, 619, 844
FaqI GGGAC 2 cut(s) 262, 391
FatI CATG 4 cut(s) 201, 466, 615, 840
FblI GTMKAC 2 cut(s) 10, 359
FokI GGATG 3 cut(s) 17, 859, 1003
FriOI GRGCYC 1 cut(s) 821
FspBI CTAG 1 cut(s) 641
GlaI GCGC 7 cut(s) 333, 339, 406, 662, 833, 852, 1014
GsuI CTGGAG 1 cut(s) 1098
HaeII RGCGCY 4 cut(s) 335, 408, 854, 1016
HaeIII GGCC 5 cut(s) 92, 392, 672, 801, 1103
HapII CCGG 4 cut(s) 350, 402, 542, 873
HgaI GACGC 1 cut(s) 268
HhaI GCGC 7 cut(s) 334, 340, 407, 663, 834, 853, 1015
Hin1I GRCGYC 3 cut(s) 260, 405, 851
Hin1II CATG 4 cut(s) 205, 470, 619, 844
Hin6I GCGC 7 cut(s) 332, 338, 405, 661, 832, 851, 1013
HinP1I GCGC 7 cut(s) 332, 338, 405, 661, 832, 851, 1013
HinfI GANTC 4 cut(s) 121, 170, 269, 1159
HpaII CCGG 4 cut(s) 350, 402, 542, 873
HphI GGTGA 2 cut(s) 289, 796
Hpy166II GTNNAC 3 cut(s) 11, 360, 885
Hpy188I TCNGA 7 cut(s) 112, 126, 175, 268, 388, 766, 865
Hpy188III TCNNGA 7 cut(s) 116, 247, 542, 616, 685, 1058, 1139
Hpy8I GTNNAC 3 cut(s) 11, 360, 885
Hpy99I CGWCG 5 cut(s) 262, 349, 650, 770, 865
HpyAV CCTTC 7 cut(s) 72, 154, 159, 783, 791, 990, 1172
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4V TGCA 3 cut(s) 83, 720, 955
HpyF3I CTNAG 8 cut(s) 20, 97, 172, 297, 898, 1009, 1118, 1173
Hsp92I GRCGYC 3 cut(s) 260, 405, 851
Hsp92II CATG 4 cut(s) 205, 470, 619, 844
HspAI GCGC 7 cut(s) 332, 338, 405, 661, 832, 851, 1013
KasI GGCGCC 2 cut(s) 404, 850
KflI GGGWCCC 1 cut(s) 249
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 4 cut(s) 112, 441, 538, 822
LmnI GCTCC 6 cut(s) 235, 421, 490, 598, 705, 865
Lsp1109I GCAGC 7 cut(s) 313, 316, 319, 843, 849, 946, 1134
LweI GCATC 3 cut(s) 171, 279, 517
MaeI CTAG 1 cut(s) 641
MaeIII GTNAC 2 cut(s) 277, 784
MalI GATC 4 cut(s) 114, 443, 540, 824
MboI GATC 4 cut(s) 112, 441, 538, 822
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 1 cut(s) 821
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 3 cut(s) 560, 1096, 1111
MluNI TGGCCA 1 cut(s) 92
Mly113I GGCGCC 2 cut(s) 405, 851
MlyI GAGTC 2 cut(s) 164, 263
MmeI TCCRAC 2 cut(s) 789, 843
Mox20I TGGCCA 1 cut(s) 92
MroI TCCGGA 1 cut(s) 541
MscI TGGCCA 1 cut(s) 92
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 856
MspI CCGG 4 cut(s) 350, 402, 542, 873
MspR9I CCNGG 3 cut(s) 536, 1034, 1047
MunI CAATTG 1 cut(s) 560
MvaI CCWGG 3 cut(s) 536, 1034, 1047
MvnI CGCG 2 cut(s) 832, 834
NarI GGCGCC 2 cut(s) 405, 851
NdeII GATC 4 cut(s) 112, 441, 538, 822
NlaIII CATG 4 cut(s) 205, 470, 619, 844
NlaIV GGNNCC 6 cut(s) 250, 251, 406, 540, 600, 852
NmeAIII GCCGAG 2 cut(s) 192, 627
NmuCI GTSAC 2 cut(s) 277, 784
NspV TTCGAA 1 cut(s) 1162
PagI TCATGA 1 cut(s) 615
PceI AGGCCT 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 278, 353
PfeI GAWTC 2 cut(s) 121, 1159
PflFI GACNNNGTC 1 cut(s) 262
PflMI CCANNNNNTGG 2 cut(s) 374, 1022
PleI GAGTC 2 cut(s) 164, 263
PluTI GGCGCC 2 cut(s) 408, 854
PpsI GAGTC 2 cut(s) 164, 263
PpuMI RGGWCCY 2 cut(s) 249, 759
Psp5II RGGWCCY 2 cut(s) 249, 759
Psp6I CCWGG 3 cut(s) 534, 1032, 1045
PspGI CCWGG 3 cut(s) 534, 1032, 1045
PspN4I GGNNCC 6 cut(s) 250, 251, 406, 540, 600, 852
PspPI GGNCC 2 cut(s) 249, 759
PspPPI RGGWCCY 2 cut(s) 249, 759
PsuI RGATCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 262
RsaI GTAC 3 cut(s) 49, 653, 668
RsaNI GTAC 3 cut(s) 48, 652, 667
RseI CAYNNNNRTG 1 cut(s) 845
Sau3AI GATC 4 cut(s) 112, 441, 538, 822
Sau96I GGNCC 2 cut(s) 249, 759
SchI GAGTC 2 cut(s) 164, 263
ScrFI CCNGG 3 cut(s) 536, 1034, 1047
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 3 cut(s) 171, 279, 517
SfcI CTRYAG 1 cut(s) 360
SfoI GGCGCC 2 cut(s) 406, 852
SfuI TTCGAA 1 cut(s) 1162
SgrAI CRCCGGYG 1 cut(s) 349
SinI GGWCC 2 cut(s) 249, 759
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 3 cut(s) 560, 1096, 1111
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 7 cut(s) 470, 735, 830, 834, 854, 947, 1104
SspDI GGCGCC 2 cut(s) 404, 850
SspMI CTAG 1 cut(s) 641
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 534, 1032, 1045
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 364
TaqI TCGA 5 cut(s) 168, 440, 633, 776, 1162
TasI AATT 3 cut(s) 560, 1096, 1111
TauI GCSGC 5 cut(s) 473, 832, 837, 856, 1106
TfiI GAWTC 2 cut(s) 121, 1159
TscAI CASTG 3 cut(s) 369, 892, 1081
TseFI GTSAC 2 cut(s) 277, 784
TseI GCWGC 7 cut(s) 301, 304, 307, 837, 856, 934, 1147
Tsp45I GTSAC 2 cut(s) 277, 784
TspDTI ATGAA 3 cut(s) 190, 474, 604
TspGWI ACGGA 3 cut(s) 236, 333, 1143
TspRI CASTG 3 cut(s) 369, 892, 1081
Tth111I GACNNNGTC 1 cut(s) 262
Van91I CCANNNNNTGG 2 cut(s) 374, 1022
VpaK11BI GGWCC 2 cut(s) 249, 759
XmiI GTMKAC 2 cut(s) 10, 359
XspI CTAG 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.