Rmu_sc0007429.1_g000003

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007429.1
OG0007203
Physical Location & Seq
Reverse (-)
4924 .. 9962
5039 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
atgagaaaagcttcggcaatctcagcgatcctatttgggctcgttcgcggcccaaaatccaattccagcttcaatgcaattcgctgttactgtgatcgaacagtttccaactccaggaagcttccagggccattaacgcagtacaagaggttagttgagcaagggaagctgcaacatgatccgaaccaagaagcggtggcttcgcaattggagaacttgctggggaggttagagcagtatgagagagatatggaggaatatcatacgagcctggcgaattgggagaagaagagagagaatgagcggcggaggattttaatggaggaagctgagagtaagcagaaggatgacgtgtggacgtcagttaacaaccaagggaataagcttttccggagatggatgtccaagaaaagacctgagaatgtagaacctggagttgggaaatgggtttcgtacttaaatagagaaaggaagttggattcaattgttggacgtcgtccaattgctcctgcagctccgaaaggactgtatatttatggcaatgttggaagtggaaagacgatgcttatggacatgttctatagtgccactgaaggaatagttaagcatcgaaagagatatcactttcatgaggctatgttgaaaattaatgaacaaatgcatcagatttggaaaaatcaagtggcagataagtctttgcagtccagcattgctaattggataatgagtctacccttcgattcgaaggtcaaggagtgggtggctgcagaagagaactacaaacaacaggttcagatgaaaaacatccttccagctgtggcagataagcttctcctagatcggcaggcagatcaaaagggtggcagcattctttgttttgatgagatacagactgttgatgtatttgctattgtggccttatctggaattgttagcagattgctaagtactggaactgtttttgtggccaccagtaaccgagcaccaacggacttaaatcaggtttcacgactgattgaaatgcagacacctgtatacttggggggagttcatagtcttcatccttatttccagagacaacgtcaagaatttgaaaatattactgcagaaaacttacagcctcagttgtctttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

378

Amino Acids

43.42

Weight (kDa)

9.46

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 158 - 333 6.9e-22 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 362, 496
AccBSI CCGCTC 1 cut(s) 304
AccI GTMKAC 2 cut(s) 730, 1035
AccII CGCG 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 390
AciI CCGC 4 cut(s) 48, 194, 304, 307
AclWI GGATC 2 cut(s) 22, 173
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 1 cut(s) 1088
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 2 cut(s) 359, 493
AfaI GTAC 3 cut(s) 143, 455, 949
AfiI CCNNNNNNNGG 3 cut(s) 114, 193, 437
AflIII ACRYGT 2 cut(s) 351, 573
AgsI TTSAA 5 cut(s) 73, 483, 643, 1019, 1094
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 4 cut(s) 113, 124, 270, 430
AjuI GAANNNNNNNTTGG 6 cut(s) 46, 53, 78, 85, 420, 452
AluBI AGCT 9 cut(s) 11, 69, 121, 169, 329, 385, 515, 815, 829
AluI AGCT 9 cut(s) 11, 69, 121, 169, 329, 385, 515, 815, 829
Alw21I GWGCWC 1 cut(s) 985
Alw26I GTCTC 1 cut(s) 1069
AlwI GGATC 2 cut(s) 22, 173
Aor13HI TCCGGA 1 cut(s) 390
AoxI GGCC 4 cut(s) 49, 128, 915, 966
ApeKI GCWGC 4 cut(s) 169, 512, 764, 864
ApoI RAATTY 1 cut(s) 1088
AseI ATTAAT 1 cut(s) 648
Asp700I GAANNNNTTC 2 cut(s) 10, 103
AspS9I GGNCC 2 cut(s) 50, 128
AsuII TTCGAA 1 cut(s) 743
BalI TGGCCA 1 cut(s) 968
BanII GRGCYC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 1049
Bbv12I GWGCWC 1 cut(s) 985
BbvI GCAGC 4 cut(s) 156, 524, 751, 876
BccI CCATC 1 cut(s) 390
BciT130I CCWGG 4 cut(s) 115, 126, 272, 432
BcoDI GTCTC 1 cut(s) 1069
BfaI CTAG 1 cut(s) 836
BfmI CTRYAG 4 cut(s) 510, 580, 765, 1104
BisI GCNGC 6 cut(s) 49, 170, 305, 513, 765, 865
BlsI GCNGC 6 cut(s) 50, 171, 306, 514, 766, 866
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 4 cut(s) 115, 126, 272, 432
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 50, 128
BmrFI CCNGG 4 cut(s) 115, 126, 272, 432
BmsI GCATC 3 cut(s) 552, 616, 670
BpiI GAAGAC 1 cut(s) 1049
BpmI CTGGAG 2 cut(s) 97, 453
Bpu14I TTCGAA 1 cut(s) 743
BsaHI GRCGYC 2 cut(s) 359, 493
BsaJI CCNNGG 2 cut(s) 125, 373
BsaWI WCCGGW 1 cut(s) 390
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 193, 437
Bse1I ACTGG 2 cut(s) 955, 972
Bse3DI GCAATG 2 cut(s) 547, 708
BseAI TCCGGA 1 cut(s) 390
BseBI CCWGG 4 cut(s) 115, 126, 272, 432
BseDI CCNNGG 2 cut(s) 125, 373
BseGI GGATG 4 cut(s) 352, 405, 804, 1060
BseLI CCNNNNNNNGG 3 cut(s) 114, 193, 437
BseMI GCAATG 2 cut(s) 547, 708
BseMII CTCAG 4 cut(s) 36, 321, 408, 1136
BseNI ACTGG 2 cut(s) 955, 972
BseXI GCAGC 4 cut(s) 156, 524, 751, 876
BseYI CCCAGC 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 4 cut(s) 51, 130, 917, 968
BsiHKAI GWGCWC 1 cut(s) 985
BsiSI CCGG 1 cut(s) 391
BslI CCNNNNNNNGG 3 cut(s) 114, 193, 437
BsmAI GTCTC 1 cut(s) 1069
BsmI GAATGC 1 cut(s) 867
BsnI GGCC 4 cut(s) 51, 130, 917, 968
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 2 cut(s) 42, 985
Bsp13I TCCGGA 1 cut(s) 390
Bsp143I GATC 5 cut(s) 27, 94, 178, 838, 850
BspACI CCGC 4 cut(s) 48, 194, 304, 307
BspANI GGCC 4 cut(s) 51, 130, 917, 968
BspCNI CTCAG 4 cut(s) 35, 322, 409, 1135
BspEI TCCGGA 1 cut(s) 390
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 628
BspMAI CTGCAG 3 cut(s) 514, 769, 1108
BspPI GGATC 2 cut(s) 22, 173
BspT104I TTCGAA 1 cut(s) 743
BsrBI CCGCTC 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 547, 708
BsrI ACTGG 2 cut(s) 955, 972
BssECI CCNNGG 2 cut(s) 125, 373
BssMI GATC 5 cut(s) 27, 94, 178, 838, 850
BssNAI GTATAC 1 cut(s) 1036
BssNI GRCGYC 2 cut(s) 359, 493
BssT1I CCWWGG 1 cut(s) 373
Bst1107I GTATAC 1 cut(s) 1036
Bst2UI CCWGG 4 cut(s) 115, 126, 272, 432
Bst4CI ACNGT 5 cut(s) 92, 103, 528, 895, 958
Bst6I CTCTTC 2 cut(s) 284, 765
BstACI GRCGYC 2 cut(s) 359, 493
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 846
BstDEI CTNAG 5 cut(s) 22, 330, 417, 944, 1122
BstF5I GGATG 4 cut(s) 352, 405, 804, 1060
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 5 cut(s) 30, 97, 181, 841, 853
BstMAI GTCTC 1 cut(s) 1069
BstMBI GATC 5 cut(s) 27, 94, 178, 838, 850
BstMWI GCNNNNNNNGC 6 cut(s) 23, 127, 136, 166, 512, 914
BstNI CCWGG 4 cut(s) 115, 126, 272, 432
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 4 cut(s) 113, 124, 270, 430
BstSFI CTRYAG 4 cut(s) 510, 580, 765, 1104
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 4 cut(s) 156, 524, 751, 876
BstV2I GAAGAC 1 cut(s) 1049
BstZ17I GTATAC 1 cut(s) 1036
BsuRI GGCC 4 cut(s) 51, 130, 917, 968
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 4 cut(s) 352, 405, 804, 1060
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 1 cut(s) 846
CciI TCATGA 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 50, 128
Csp6I GTAC 3 cut(s) 142, 454, 948
CviAII CATG 3 cut(s) 176, 574, 629
CviQI GTAC 3 cut(s) 142, 454, 948
DdeI CTNAG 5 cut(s) 22, 330, 417, 944, 1122
DpnI GATC 5 cut(s) 29, 96, 180, 840, 852
DpnII GATC 5 cut(s) 27, 94, 178, 838, 850
EaeI YGGCCR 1 cut(s) 966
Eam1104I CTCTTC 2 cut(s) 284, 765
EarI CTCTTC 2 cut(s) 284, 765
EciI GGCGGA 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 42
Eco32I GATATC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 612
EcoRII CCWGG 4 cut(s) 113, 124, 270, 430
EcoRV GATATC 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 3 cut(s) 179, 577, 632
FatI CATG 3 cut(s) 175, 573, 628
FblI GTMKAC 2 cut(s) 730, 1035
Fnu4HI GCNGC 6 cut(s) 49, 170, 305, 513, 765, 865
FokI GGATG 4 cut(s) 359, 412, 791, 1047
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 6 cut(s) 49, 170, 305, 513, 765, 865
FspBI CTAG 1 cut(s) 836
GluI GCNGC 6 cut(s) 49, 170, 305, 513, 765, 865
GsaI CCCAGC 1 cut(s) 224
GsuI CTGGAG 2 cut(s) 97, 453
HaeIII GGCC 4 cut(s) 51, 130, 917, 968
HapII CCGG 1 cut(s) 391
Hin1I GRCGYC 2 cut(s) 359, 493
Hin1II CATG 3 cut(s) 179, 577, 632
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 4 cut(s) 9, 119, 383, 827
HinfI GANTC 3 cut(s) 479, 727, 740
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 1 cut(s) 391
Hpy166II GTNNAC 4 cut(s) 357, 367, 731, 1036
Hpy188I TCNGA 4 cut(s) 183, 519, 666, 795
Hpy188III TCNNGA 6 cut(s) 391, 629, 924, 1008, 1072, 1085
Hpy8I GTNNAC 4 cut(s) 357, 367, 731, 1036
Hpy99I CGWCG 1 cut(s) 498
HpyAV CCTTC 5 cut(s) 337, 587, 739, 745, 818
HpyCH4III ACNGT 5 cut(s) 92, 103, 528, 895, 958
HpyCH4IV ACGT 4 cut(s) 351, 359, 493, 1081
HpyCH4V TGCA 8 cut(s) 77, 172, 512, 661, 700, 767, 1024, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 127, 136, 166, 512, 914
HpyF3I CTNAG 5 cut(s) 22, 330, 417, 944, 1122
HpySE526I ACGT 4 cut(s) 351, 359, 493, 1081
Hsp92I GRCGYC 2 cut(s) 359, 493
Hsp92II CATG 3 cut(s) 179, 577, 632
Kpn2I TCCGGA 1 cut(s) 390
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 5 cut(s) 27, 94, 178, 838, 850
LmnI GCTCC 2 cut(s) 511, 520
Lsp1109I GCAGC 4 cut(s) 156, 524, 751, 876
LweI GCATC 3 cut(s) 552, 616, 670
MaeI CTAG 1 cut(s) 836
MaeII ACGT 4 cut(s) 351, 359, 493, 1081
MaeIII GTNAC 2 cut(s) 86, 974
MalI GATC 5 cut(s) 29, 96, 180, 840, 852
MbiI CCGCTC 1 cut(s) 304
MboI GATC 5 cut(s) 27, 94, 178, 838, 850
MboII GAAGA 4 cut(s) 298, 301, 782, 1049
MfeI CAATTG 3 cut(s) 206, 483, 501
MhlI GDGCHC 2 cut(s) 42, 985
MlsI TGGCCA 1 cut(s) 968
MluNI TGGCCA 1 cut(s) 968
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 4 cut(s) 132, 456, 469, 526
MnlI CCTC 7 cut(s) 141, 219, 247, 303, 316, 625, 1131
Mox20I TGGCCA 1 cut(s) 968
Mph1103I ATGCAT 1 cut(s) 663
MroI TCCGGA 1 cut(s) 390
MroXI GAANNNNTTC 2 cut(s) 10, 103
MscI TGGCCA 1 cut(s) 968
MseI TTAA 7 cut(s) 134, 317, 366, 458, 603, 648, 995
MslI CAYNNNNRTG 1 cut(s) 627
Msp20I TGGCCA 1 cut(s) 968
MspA1I CMGCKG 1 cut(s) 815
MspI CCGG 1 cut(s) 391
MspR9I CCNGG 4 cut(s) 115, 126, 272, 432
MunI CAATTG 3 cut(s) 206, 483, 501
Mva1269I GAATGC 1 cut(s) 867
MvaI CCWGG 4 cut(s) 115, 126, 272, 432
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 6 cut(s) 23, 127, 136, 166, 512, 914
NdeII GATC 5 cut(s) 27, 94, 178, 838, 850
NlaIII CATG 3 cut(s) 179, 577, 632
NsiI ATGCAT 1 cut(s) 663
NspI RCATGY 1 cut(s) 577
NspV TTCGAA 1 cut(s) 743
PagI TCATGA 1 cut(s) 628
PciI ACATGT 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 272
PctI GAATGC 1 cut(s) 867
PdmI GAANNNNTTC 2 cut(s) 10, 103
PfeI GAWTC 2 cut(s) 479, 740
PflFI GACNNNGTC 2 cut(s) 495, 1080
PfoI TCCNGGA 1 cut(s) 113
PkrI GCNGC 6 cut(s) 50, 171, 306, 514, 766, 866
PleI GAGTC 1 cut(s) 735
PpsI GAGTC 1 cut(s) 735
PscI ACATGT 1 cut(s) 573
PshBI ATTAAT 1 cut(s) 648
Psp6I CCWGG 4 cut(s) 113, 124, 270, 430
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 4 cut(s) 113, 124, 270, 430
PspPI GGNCC 2 cut(s) 50, 128
PstI CTGCAG 3 cut(s) 514, 769, 1108
PsyI GACNNNGTC 2 cut(s) 495, 1080
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 3 cut(s) 143, 455, 949
RsaNI GTAC 3 cut(s) 142, 454, 948
RseI CAYNNNNRTG 1 cut(s) 627
SaqAI TTAA 7 cut(s) 134, 317, 366, 458, 603, 648, 995
SatI GCNGC 6 cut(s) 49, 170, 305, 513, 765, 865
Sau3AI GATC 5 cut(s) 27, 94, 178, 838, 850
Sau96I GGNCC 2 cut(s) 50, 128
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 4 cut(s) 115, 126, 272, 432
SduI GDGCHC 2 cut(s) 42, 985
SfaNI GCATC 3 cut(s) 552, 616, 670
SfcI CTRYAG 4 cut(s) 510, 580, 765, 1104
SfuI TTCGAA 1 cut(s) 743
SmiMI CAYNNNNRTG 1 cut(s) 627
SsiI CCGC 4 cut(s) 48, 194, 304, 307
SspI AATATT 1 cut(s) 1099
SspMI CTAG 1 cut(s) 836
StyD4I CCNGG 4 cut(s) 113, 124, 270, 430
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 5 cut(s) 92, 103, 528, 895, 958
TaiI ACGT 4 cut(s) 354, 362, 496, 1084
TaqI TCGA 4 cut(s) 97, 610, 738, 743
TatI WGTACW 2 cut(s) 141, 947
TauI GCSGC 2 cut(s) 51, 307
TfiI GAWTC 2 cut(s) 479, 740
Tru1I TTAA 7 cut(s) 134, 317, 366, 458, 603, 648, 995
Tru9I TTAA 7 cut(s) 134, 317, 366, 458, 603, 648, 995
TscAI CASTG 1 cut(s) 595
TseI GCWGC 4 cut(s) 169, 512, 764, 864
TspDTI ATGAA 5 cut(s) 617, 666, 812, 1040, 1049
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 1 cut(s) 595
Tth111I GACNNNGTC 2 cut(s) 495, 1080
VspI ATTAAT 1 cut(s) 648
XapI RAATTY 1 cut(s) 1088
XceI RCATGY 1 cut(s) 577
XmiI GTMKAC 2 cut(s) 730, 1035
XmnI GAANNNNTTC 2 cut(s) 10, 103
XspI CTAG 1 cut(s) 836
ZraI GACGTC 2 cut(s) 360, 494
ZrmI AGTACT 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.