Rmu_sc0010408.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010408.1
OG0017004
Physical Location & Seq
Forward (+)
6333 .. 6884
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010408.1_g000001.1.cds

Sequence Viewer

Length: 447 bp
atgaatcaaacttgcagaggaggtatccttggttatggttctgatgtcttccatcccagtgtccttggttcgctgttatattggatcagtggctgcgggggactggagggtttgggatccagggcttctgcaggcgtcatggtgccgagcaaggctaaaattggatttgttagacaaatatcgggtctggtggcaaagcagataggtgagaggcttgcatcgggcgattgcatgaccatgattacaggtggtgggaggatggcagatcgacggccggaatctgggcttggttttggtgttcgctatcgtgcagctttgatcagagggggttgggtcgggctgagaaacaacctacctctggttcttcatggaaaccgccccaagtctggtggggtaggtggcgacgcattgaatcgagcggccagaactgggtccgggtttctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

15.29

Weight (kDa)

10.85

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 419
AciI CCGC 3 cut(s) 96, 376, 419
AclWI GGATC 3 cut(s) 92, 111, 124
AcoI YGGCCR 2 cut(s) 272, 420
AcyI GRCGYC 1 cut(s) 135
AfiI CCNNNNNNNGG 4 cut(s) 281, 358, 386, 429
AgsI TTSAA 1 cut(s) 412
AjnI CCWGG 1 cut(s) 119
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
AlwI GGATC 3 cut(s) 92, 111, 124
AlwNI CAGNNNCTG 1 cut(s) 93
AoxI GGCC 2 cut(s) 272, 420
ApeKI GCWGC 2 cut(s) 93, 311
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AspS9I GGNCC 1 cut(s) 432
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 40
BbvI GCAGC 2 cut(s) 80, 323
BccI CCATC 2 cut(s) 60, 253
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 1 cut(s) 121
BciVI GTATCC 1 cut(s) 35
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 1 cut(s) 436
BfaI CTAG 1 cut(s) 445
BfmI CTRYAG 1 cut(s) 129
BfuI GTATCC 1 cut(s) 35
BisI GCNGC 3 cut(s) 94, 312, 420
BlsI GCNGC 3 cut(s) 95, 313, 421
Bme1390I CCNGG 2 cut(s) 121, 436
Bme18I GGWCC 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 3 cut(s) 118, 144, 433
BmrFI CCNGG 2 cut(s) 121, 436
BmrI ACTGGG 2 cut(s) 51, 438
BmsI GCATC 1 cut(s) 227
BmuI ACTGGG 2 cut(s) 51, 438
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 436
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 3 cut(s) 28, 64, 120
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 358, 386, 429
Bse1I ACTGG 3 cut(s) 57, 108, 433
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 3 cut(s) 28, 64, 120
BseGI GGATG 2 cut(s) 52, 264
BseLI CCNNNNNNNGG 4 cut(s) 281, 358, 386, 429
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 3 cut(s) 57, 108, 433
BseRI GAGGAG 1 cut(s) 33
BseX3I CGGCCG 1 cut(s) 272
BseXI GCAGC 2 cut(s) 80, 323
BsgI GTGCAG 1 cut(s) 330
Bsh1285I CGRYCG 1 cut(s) 275
BshFI GGCC 2 cut(s) 274, 422
BshNI GGYRCC 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 275
BsiSI CCGG 2 cut(s) 275, 435
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 4 cut(s) 281, 358, 386, 429
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 2 cut(s) 274, 422
Bsp143I GATC 4 cut(s) 84, 116, 265, 318
BspACI CCGC 3 cut(s) 96, 376, 419
BspANI GGCC 2 cut(s) 274, 422
BspCNI CTCAG 1 cut(s) 333
BspLI GGNNCC 3 cut(s) 118, 144, 433
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 3 cut(s) 92, 111, 124
BspT107I GGYRCC 1 cut(s) 142
BsrBI CCGCTC 1 cut(s) 419
BsrI ACTGG 3 cut(s) 57, 108, 433
BssECI CCNNGG 3 cut(s) 28, 64, 120
BssMI GATC 4 cut(s) 84, 116, 265, 318
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 28, 64
Bst2UI CCWGG 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 133, 216
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 2 cut(s) 52, 264
BstKTI GATC 4 cut(s) 87, 119, 268, 321
BstMBI GATC 4 cut(s) 84, 116, 265, 318
BstMCI CGRYCG 1 cut(s) 275
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 119, 434
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 80, 323
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BstZI CGGCCG 1 cut(s) 272
BsuI GTATCC 1 cut(s) 35
BsuRI GGCC 2 cut(s) 274, 422
BtsCI GGATG 2 cut(s) 52, 264
BtsIMutI CAGTG 2 cut(s) 64, 94
Cac8I GCNNGC 2 cut(s) 133, 216
CaiI CAGNNNCTG 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 432
CseI GACGC 2 cut(s) 124, 413
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 4 cut(s) 139, 232, 238, 368
CviJI RGCY 9 cut(s) 93, 125, 155, 214, 274, 286, 314, 340, 422
CviKI_1 RGCY 9 cut(s) 93, 125, 155, 214, 274, 286, 314, 340, 422
DdeI CTNAG 1 cut(s) 341
DpnI GATC 4 cut(s) 86, 118, 267, 320
DpnII GATC 4 cut(s) 84, 116, 265, 318
EaeI YGGCCR 2 cut(s) 272, 420
EagI CGGCCG 1 cut(s) 272
EclXI CGGCCG 1 cut(s) 272
Eco130I CCWWGG 2 cut(s) 28, 64
Eco47I GGWCC 1 cut(s) 432
Eco52I CGGCCG 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 2 cut(s) 28, 64
ErhI CCWWGG 2 cut(s) 28, 64
FaeI CATG 4 cut(s) 142, 235, 241, 371
FaiI YATR 6 cut(s) 36, 79, 140, 233, 239, 369
FaqI GGGAC 1 cut(s) 114
FatI CATG 4 cut(s) 138, 231, 237, 367
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 318
Fnu4HI GCNGC 3 cut(s) 94, 312, 420
FokI GGATG 2 cut(s) 39, 271
Fsp4HI GCNGC 3 cut(s) 94, 312, 420
FspBI CTAG 1 cut(s) 445
GluI GCNGC 3 cut(s) 94, 312, 420
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 2 cut(s) 274, 422
HapII CCGG 2 cut(s) 275, 435
HgaI GACGC 2 cut(s) 124, 413
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 4 cut(s) 142, 235, 241, 371
HinfI GANTC 3 cut(s) 4, 278, 412
HpaII CCGG 2 cut(s) 275, 435
HphI GGTGA 1 cut(s) 218
Hpy188I TCNGA 2 cut(s) 43, 323
Hpy99I CGWCG 2 cut(s) 273, 407
HpyCH4V TGCA 5 cut(s) 15, 131, 218, 231, 311
HpyF3I CTNAG 1 cut(s) 341
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 4 cut(s) 142, 235, 241, 371
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 4 cut(s) 84, 116, 265, 318
Lsp1109I GCAGC 2 cut(s) 80, 323
LweI GCATC 1 cut(s) 227
MaeI CTAG 1 cut(s) 445
MalI GATC 4 cut(s) 86, 118, 267, 320
MbiI CCGCTC 1 cut(s) 419
MboI GATC 4 cut(s) 84, 116, 265, 318
MboII GAAGA 2 cut(s) 40, 356
MflI RGATCY 1 cut(s) 116
MluCI AATT 1 cut(s) 159
MnlI CCTC 7 cut(s) 11, 14, 100, 204, 249, 317, 366
MslI CAYNNNNRTG 2 cut(s) 57, 236
MspI CCGG 2 cut(s) 275, 435
MspR9I CCNGG 2 cut(s) 121, 436
MvaI CCWGG 1 cut(s) 121
NciI CCSGG 1 cut(s) 436
NdeII GATC 4 cut(s) 84, 116, 265, 318
NlaIII CATG 4 cut(s) 142, 235, 241, 371
NlaIV GGNNCC 3 cut(s) 118, 144, 433
NmeAIII GCCGAG 1 cut(s) 171
PfeI GAWTC 3 cut(s) 4, 278, 412
PkrI GCNGC 3 cut(s) 95, 313, 421
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 3 cut(s) 118, 144, 433
PspPI GGNCC 1 cut(s) 432
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 93
PsuI RGATCY 1 cut(s) 116
RseI CAYNNNNRTG 2 cut(s) 57, 236
SatI GCNGC 3 cut(s) 94, 312, 420
Sau3AI GATC 4 cut(s) 84, 116, 265, 318
Sau96I GGNCC 1 cut(s) 432
ScrFI CCNGG 2 cut(s) 121, 436
SetI ASST 7 cut(s) 25, 208, 250, 316, 354, 358, 400
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 1 cut(s) 432
SmiMI CAYNNNNRTG 2 cut(s) 57, 236
Sse9I AATT 1 cut(s) 159
SsiI CCGC 3 cut(s) 96, 376, 419
SspMI CTAG 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 119, 434
StyI CCWWGG 2 cut(s) 28, 64
TaqI TCGA 2 cut(s) 268, 415
TasI AATT 1 cut(s) 159
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 3 cut(s) 4, 278, 412
TscAI CASTG 2 cut(s) 64, 94
TseI GCWGC 2 cut(s) 93, 311
TspDTI ATGAA 2 cut(s) 17, 356
TspRI CASTG 2 cut(s) 64, 94
VpaK11BI GGWCC 1 cut(s) 432
XspI CTAG 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.