Rroxscaffold_1G00008120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017004
Physical Location & Seq
Reverse (-)
10249658 .. 10254854
5197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008120.1

Sequence Viewer

Length: 777 bp
ATGGCGGAGCATGGAGTGTTCAATGAATGGACCTATTTCTATGTACCACTGTGGGTACTACCACATCTTCTTGTTCGTCCGTATATGGCAGCGGAAGAGATGTTATTCATTCCTCTTGAGACTTTGCAATTTTTGTATGAACCCAATGTTGTTAGGGTTCGCATTTGGGATTCAGGAGATTTTTTATGGGCCCCAAATATTTCTGATTCTTGGAATTGGTGTGTTGGTTACATAGGTAGAAGATTGCTTTCTATTCAACGTATTAATTTGCGTGGAGTAGGTAAGGTCGGTCACACTACCGCCGGTTGCCTTGATAGAAGGTTACCCTCTATTCAACGCATTTCTTTGCGTGGAAATATGGTTGACCATACTATTGGTACTATTTTACATAGCCCGGATTCTGTCCGGTGTTTCATCAAATGCTTAATTGCATCCGTTCGTATCCTTGCTAGGTTTTATTTCCGATGCTTTGTTGCATCTACTCTACTTTCAACCCCTCAAATCTGTAGTCGGGAATCCTACGTACTCATTCACAAGCAATGCTTCAAAGATGTGCAAACCGAAGACAAATGTGGCAACTCAGAGTTCAAGATTGTAAACATAAAGAGTGAATATGAAGCTCTTGAGGTTAAGTTTCGGCCTTTCAAATGCGAAAAACTTGCAATGGAAGAACACATTAGAGCACTCATCTCTTCTTCCATAGCAAGCTTTTCACCTTCCAAAGGCCGAAACTTAACCTCGAGAGCTTTATACTCACTCTTTATCTTCAACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

29.87

Weight (kDa)

9.06

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 92, 300
AfaI GTAC 4 cut(s) 45, 57, 379, 525
AfiI CCNNNNNNNGG 1 cut(s) 722
AgsI TTSAA 8 cut(s) 22, 257, 335, 492, 547, 589, 646, 769
AluBI AGCT 3 cut(s) 620, 708, 746
AluI AGCT 3 cut(s) 620, 708, 746
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 3 cut(s) 189, 638, 724
ApaI GGGCCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AseI ATTAAT 1 cut(s) 264
AspS9I GGNCC 3 cut(s) 30, 189, 190
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 1 cut(s) 30
BaeGI GKGCMC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciVI GTATCC 1 cut(s) 452
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 2 cut(s) 450, 775
BfmI CTRYAG 1 cut(s) 505
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 739
BmgT120I GGNCC 3 cut(s) 30, 189, 190
BmiI GGNNCC 2 cut(s) 191, 192
BmrFI CCNGG 1 cut(s) 395
BmsI GCATC 3 cut(s) 440, 455, 485
BpiI GAAGAC 1 cut(s) 570
BpuEI CTTGAG 2 cut(s) 137, 644
BpuMI CCSGG 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 523
BsaWI WCCGGW 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse118I RCCGGY 1 cut(s) 302
Bse3DI GCAATG 2 cut(s) 545, 669
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMI GCAATG 2 cut(s) 545, 669
BseMII CTCAG 1 cut(s) 594
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 1 cut(s) 101
BshFI GGCC 3 cut(s) 191, 640, 726
BsiHKAI GWGCWC 1 cut(s) 685
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 3 cut(s) 303, 395, 406
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 1 cut(s) 113
BsnI GGCC 3 cut(s) 191, 640, 726
BsoBI CYCGRG 1 cut(s) 739
Bsp120I GGGCCC 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 193, 685
BspACI CCGC 3 cut(s) 5, 92, 300
BspANI GGCC 3 cut(s) 191, 640, 726
BspCNI CTCAG 1 cut(s) 593
BspLI GGNNCC 2 cut(s) 191, 192
BsrDI GCAATG 2 cut(s) 545, 669
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 51
Bst6I CTCTTC 2 cut(s) 90, 697
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 1 cut(s) 580
BstEII GGTNACC 1 cut(s) 321
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 1 cut(s) 431
BstMAI GTCTC 1 cut(s) 113
BstPI GGTNACC 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 193
BstSNI TACGTA 1 cut(s) 523
BstV1I GCAGC 1 cut(s) 101
BstV2I GAAGAC 1 cut(s) 570
BstXI CCANNNNNNTGG 1 cut(s) 374
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 3 cut(s) 191, 640, 726
BtsCI GGATG 1 cut(s) 431
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 706
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 30, 189, 190
Csp6I GTAC 4 cut(s) 44, 56, 378, 524
CviAII CATG 1 cut(s) 11
CviJI RGCY 7 cut(s) 191, 393, 620, 640, 708, 726, 746
CviKI_1 RGCY 7 cut(s) 191, 393, 620, 640, 708, 726, 746
CviQI GTAC 4 cut(s) 44, 56, 378, 524
DdeI CTNAG 1 cut(s) 580
Eam1104I CTCTTC 2 cut(s) 90, 697
EarI CTCTTC 2 cut(s) 90, 697
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 739
Eco91I GGTNACC 1 cut(s) 321
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 190
EcoO65I GGTNACC 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 1 cut(s) 14
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FatI CATG 1 cut(s) 10
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 1 cut(s) 418
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 1 cut(s) 90
FspBI CTAG 2 cut(s) 450, 775
GluI GCNGC 1 cut(s) 90
HaeIII GGCC 3 cut(s) 191, 640, 726
HapII CCGG 3 cut(s) 303, 395, 406
Hin1II CATG 1 cut(s) 14
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 706
HinfI GANTC 4 cut(s) 170, 206, 398, 515
HpaII CCGG 3 cut(s) 303, 395, 406
HphI GGTGA 1 cut(s) 705
Hpy166II GTNNAC 2 cut(s) 364, 598
Hpy188I TCNGA 3 cut(s) 205, 464, 583
Hpy188III TCNNGA 6 cut(s) 116, 174, 512, 589, 623, 741
Hpy8I GTNNAC 2 cut(s) 364, 598
HpyAV CCTTC 2 cut(s) 312, 726
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 2 cut(s) 259, 522
HpyCH4V TGCA 5 cut(s) 127, 431, 476, 556, 662
HpyF3I CTNAG 1 cut(s) 580
HpySE526I ACGT 2 cut(s) 259, 522
Hsp92II CATG 1 cut(s) 14
LmnI GCTCC 1 cut(s) 7
LpnPI CCDG 4 cut(s) 159, 316, 408, 419
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 3 cut(s) 440, 455, 485
MaeI CTAG 2 cut(s) 450, 775
MaeII ACGT 2 cut(s) 259, 522
MaeIII GTNAC 3 cut(s) 227, 290, 321
MboII GAAGA 8 cut(s) 59, 107, 252, 575, 680, 684, 687, 757
MhlI GDGCHC 2 cut(s) 193, 685
MluCI AATT 4 cut(s) 128, 214, 265, 426
MnlI CCTC 5 cut(s) 123, 337, 507, 619, 748
MseI TTAA 4 cut(s) 264, 425, 630, 734
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 3 cut(s) 303, 395, 406
MspR9I CCNGG 1 cut(s) 395
NciI CCSGG 1 cut(s) 395
NlaIII CATG 1 cut(s) 14
NlaIV GGNNCC 2 cut(s) 191, 192
NmuCI GTSAC 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 739
PfeI GAWTC 4 cut(s) 170, 206, 398, 515
PkrI GCNGC 1 cut(s) 91
Ppu21I YACGTR 1 cut(s) 523
PshBI ATTAAT 1 cut(s) 264
PspEI GGTNACC 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 191, 192
PspOMI GGGCCC 1 cut(s) 189
PspPI GGNCC 3 cut(s) 30, 189, 190
RsaI GTAC 4 cut(s) 45, 57, 379, 525
RsaNI GTAC 4 cut(s) 44, 56, 378, 524
SaqAI TTAA 4 cut(s) 264, 425, 630, 734
SatI GCNGC 1 cut(s) 90
Sau96I GGNCC 3 cut(s) 30, 189, 190
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 2 cut(s) 193, 685
SfaNI GCATC 3 cut(s) 440, 455, 485
SfcI CTRYAG 1 cut(s) 505
Sfr274I CTCGAG 1 cut(s) 739
SinI GGWCC 1 cut(s) 30
SlaI CTCGAG 1 cut(s) 739
SmlI CTYRAG 3 cut(s) 116, 623, 739
SmoI CTYRAG 3 cut(s) 116, 623, 739
SnaBI TACGTA 1 cut(s) 523
Sse9I AATT 4 cut(s) 128, 214, 265, 426
SsiI CCGC 3 cut(s) 5, 92, 300
SspI AATATT 1 cut(s) 199
SspMI CTAG 2 cut(s) 450, 775
StyD4I CCNGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 2 cut(s) 262, 525
TaqI TCGA 1 cut(s) 740
TaqII GACCGA 1 cut(s) 278
TasI AATT 4 cut(s) 128, 214, 265, 426
TfiI GAWTC 4 cut(s) 170, 206, 398, 515
Tru1I TTAA 4 cut(s) 264, 425, 630, 734
Tru9I TTAA 4 cut(s) 264, 425, 630, 734
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 5 cut(s) 39, 97, 153, 403, 630
TspGWI ACGGA 2 cut(s) 69, 424
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 30
VspI ATTAAT 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 720
XhoI CTCGAG 1 cut(s) 739
XspI CTAG 2 cut(s) 450, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.