Rmu_sc0013620.1_g000003

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013620.1
OG0018250
Physical Location & Seq
Reverse (-)
2887 .. 5944
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013620.1_g000003.1.cds

Sequence Viewer

Length: 1014 bp
atggatgtcgcactcgttgccggcgacgaagcaggttttgggggaggagagagacctgggctccggcgtcctcgtactctctgtcctcgatgtcggcgaggatcaggtcgagagcgtaaaggggagatagagatggatactcaagaatttctcaaatttactagacattttgttgtaggtcctttgtcatttgactatgcacactcgtttgacaataagccgcaactgaagttgaacccatactcgtggacaaaggttgccaacataatatttatagatgcccctgtgggcacagggttctcatatacaaacagttgggaagaataccccactctgaatgacacgatatcatcggcacaaacatatgagtttcttagaaagtctcacaaacctagaagacccaaattgactacaaacccgcttcccatccccaccgccccggaaatctccacacttccatctccagaccaccctctctcgccggtcaagagaaaacccagttcaccgatatctaatacccgaaaacccaaaactcatctcgacgactcaacctccatccgaggaggactatggccatcttcaacaactccttccctgaagaacttcgactctctagcaaactcaactcggcgaagtttcgatagtttctctgatgctctcgggtcgagatcaaagtgtagcgacatcggttctggcacgaagatcaatattgggtggacgagtttcggggctcagagctcaacggttagttcaagaaagttggaggtggaggctctgtctaagtacatgagtgatattcagacgaggcatcattggaggcggacgtcggagacgcaggctgcagcttcgacctccctcaccttcagcttccagatcccggccatcgccaaccggttcaagtccatatcgcctcagccggagagtccgaacttgaatcttgcccacgcctcgctagacaacccagaaaggtggcccgcttctgataaagctggcccgagacaccatcacctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

337

Amino Acids

37.42

Weight (kDa)

9.77

Isoelectric Point (pI)

56.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 827
Acc36I ACCTGC 1 cut(s) 23
AciI CCGC 5 cut(s) 221, 419, 435, 820, 975
AclWI GGATC 2 cut(s) 109, 868
AcoI YGGCCR 2 cut(s) 572, 879
AcsI RAATTY 2 cut(s) 146, 155
AcuI CTGAAG 3 cut(s) 248, 617, 847
AcyI GRCGYC 2 cut(s) 67, 824
AfaI GTAC 2 cut(s) 76, 785
AfiI CCNNNNNNNGG 1 cut(s) 877
AgeI ACCGGT 1 cut(s) 891
AgsI TTSAA 5 cut(s) 235, 582, 753, 898, 934
AjnI CCWGG 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 21, 53
AluBI AGCT 4 cut(s) 738, 845, 867, 989
AluI AGCT 4 cut(s) 738, 845, 867, 989
Alw21I GWGCWC 1 cut(s) 740
Alw26I GTCTC 4 cut(s) 46, 387, 824, 991
AlwI GGATC 2 cut(s) 109, 868
Ama87I CYCGRG 2 cut(s) 659, 994
AoxI GGCC 4 cut(s) 572, 879, 971, 991
ApeKI GCWGC 2 cut(s) 839, 842
ApoI RAATTY 2 cut(s) 146, 155
AsiGI ACCGGT 1 cut(s) 891
Asp700I GAANNNNTTC 1 cut(s) 602
AspS9I GGNCC 3 cut(s) 179, 972, 992
AsuC2I CCSGG 2 cut(s) 440, 878
AsuHPI GGTGA 3 cut(s) 495, 850, 998
AvaI CYCGRG 2 cut(s) 659, 994
AvaII GGWCC 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 293
BalI TGGCCA 1 cut(s) 574
BanII GRGCYC 3 cut(s) 63, 733, 740
BauI CACGAG 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 740
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 2 cut(s) 826, 854
BccI CCATC 7 cut(s) 127, 434, 466, 563, 583, 890, 1011
BciT130I CCWGG 1 cut(s) 57
BciVI GTATCC 1 cut(s) 130
BcnI CCSGG 2 cut(s) 440, 878
BcoDI GTCTC 4 cut(s) 46, 387, 824, 991
BfaI CTAG 4 cut(s) 162, 393, 614, 953
BfmI CTRYAG 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 1 cut(s) 130
BisI GCNGC 3 cut(s) 221, 840, 843
BlsI GCNGC 3 cut(s) 222, 841, 844
Bme1390I CCNGG 3 cut(s) 57, 440, 878
Bme18I GGWCC 1 cut(s) 179
BmeT110I CYCGRG 2 cut(s) 659, 994
BmgT120I GGNCC 3 cut(s) 179, 972, 992
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 3 cut(s) 57, 440, 878
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 3 cut(s) 268, 643, 817
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 403
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 912
BpuEI CTTGAG 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 440, 878
BsaHI GRCGYC 2 cut(s) 67, 824
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 56, 438, 559
BsaWI WCCGGW 1 cut(s) 891
BsaXI ACNNNNNCTCC 6 cut(s) 45, 75, 536, 566, 761, 791
Bsc4I CCNNNNNNNGG 1 cut(s) 877
Bse118I RCCGGY 3 cut(s) 20, 481, 891
Bse1I ACTGG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 3 cut(s) 56, 438, 559
BseGI GGATG 3 cut(s) 10, 426, 555
BseLI CCNNNNNNNGG 1 cut(s) 877
BseMII CTCAG 2 cut(s) 746, 926
BseNI ACTGG 1 cut(s) 498
BseRI GAGGAG 2 cut(s) 60, 576
BseSI GKGCMC 1 cut(s) 293
BseXI GCAGC 2 cut(s) 826, 854
BshFI GGCC 4 cut(s) 574, 881, 973, 993
BshTI ACCGGT 1 cut(s) 891
BsiHKAI GWGCWC 1 cut(s) 740
BsiHKCI CYCGRG 2 cut(s) 659, 994
BsiSI CCGG 7 cut(s) 21, 64, 440, 482, 878, 892, 917
BslI CCNNNNNNNGG 1 cut(s) 877
BsmAI GTCTC 4 cut(s) 46, 387, 824, 991
BsmBI CGTCTC 1 cut(s) 824
BsnI GGCC 4 cut(s) 574, 881, 973, 993
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 2 cut(s) 659, 994
Bsp1286I GDGCHC 4 cut(s) 63, 293, 733, 740
Bsp143I GATC 4 cut(s) 101, 668, 702, 873
BspACI CCGC 5 cut(s) 221, 419, 435, 820, 975
BspANI GGCC 4 cut(s) 574, 881, 973, 993
BspCNI CTCAG 2 cut(s) 745, 925
BspLI GGNNCC 1 cut(s) 62
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 109, 868
BspTNI GGTCTC 1 cut(s) 46
BsrFI RCCGGY 3 cut(s) 20, 481, 891
BsrI ACTGG 1 cut(s) 498
BssAI RCCGGY 3 cut(s) 20, 481, 891
BssECI CCNNGG 3 cut(s) 56, 438, 559
BssMI GATC 4 cut(s) 101, 668, 702, 873
BssNI GRCGYC 2 cut(s) 67, 824
BssSI CACGAG 1 cut(s) 244
Bst2BI CACGAG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 2 cut(s) 314, 745
BstACI GRCGYC 2 cut(s) 67, 824
BstAPI GCANNNNNTGC 1 cut(s) 17
BstC8I GCNNGC 4 cut(s) 22, 837, 975, 991
BstDEI CTNAG 4 cut(s) 374, 732, 780, 912
BstF5I GGATG 3 cut(s) 10, 426, 555
BstKTI GATC 4 cut(s) 104, 671, 705, 876
BstMAI GTCTC 4 cut(s) 46, 387, 824, 991
BstMBI GATC 4 cut(s) 101, 668, 702, 873
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 3 cut(s) 55, 438, 876
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 826, 854
BstV2I GAAGAC 1 cut(s) 403
BstX2I RGATCY 1 cut(s) 873
BstXI CCANNNNNNTGG 2 cut(s) 246, 969
BstYI RGATCY 1 cut(s) 873
BsuI GTATCC 1 cut(s) 130
BsuRI GGCC 4 cut(s) 574, 881, 973, 993
BtgZI GCGATG 1 cut(s) 868
BtsCI GGATG 3 cut(s) 10, 426, 555
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 4 cut(s) 22, 837, 975, 991
Cfr10I RCCGGY 3 cut(s) 20, 481, 891
Cfr13I GGNCC 3 cut(s) 179, 972, 992
CseI GACGC 2 cut(s) 56, 841
Csp6I GTAC 2 cut(s) 75, 784
CspAI ACCGGT 1 cut(s) 891
CviAII CATG 1 cut(s) 787
CviQI GTAC 2 cut(s) 75, 784
DdeI CTNAG 4 cut(s) 374, 732, 780, 912
DpnI GATC 4 cut(s) 103, 670, 704, 875
DpnII GATC 4 cut(s) 101, 668, 702, 873
EaeI YGGCCR 2 cut(s) 572, 879
EciI GGCGGA 1 cut(s) 835
Ecl136II GAGCTC 1 cut(s) 738
Eco24I GRGCYC 3 cut(s) 63, 733, 740
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 2 cut(s) 348, 510
Eco47I GGWCC 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 738
Eco57I CTGAAG 3 cut(s) 248, 617, 847
Eco88I CYCGRG 2 cut(s) 659, 994
EcoICRI GAGCTC 1 cut(s) 738
EcoO109I RGGNCCY 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 55
EcoRV GATATC 2 cut(s) 348, 510
EcoT38I GRGCYC 3 cut(s) 63, 733, 740
Esp3I CGTCTC 1 cut(s) 824
FaeI CATG 1 cut(s) 790
FatI CATG 1 cut(s) 786
FauI CCCGC 2 cut(s) 426, 982
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 221, 840, 843
FokI GGATG 3 cut(s) 17, 413, 542
FriOI GRGCYC 3 cut(s) 63, 733, 740
Fsp4HI GCNGC 3 cut(s) 221, 840, 843
FspBI CTAG 4 cut(s) 162, 393, 614, 953
GluI GCNGC 3 cut(s) 221, 840, 843
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 4 cut(s) 574, 881, 973, 993
HapII CCGG 7 cut(s) 21, 64, 440, 482, 878, 892, 917
HgaI GACGC 2 cut(s) 56, 841
Hin1I GRCGYC 2 cut(s) 67, 824
Hin1II CATG 1 cut(s) 790
HinfI GANTC 4 cut(s) 545, 608, 922, 934
HpaII CCGG 7 cut(s) 21, 64, 440, 482, 878, 892, 917
HphI GGTGA 3 cut(s) 495, 850, 998
Hpy166II GTNNAC 3 cut(s) 249, 503, 717
Hpy188I TCNGA 9 cut(s) 336, 560, 652, 735, 801, 829, 927, 982, 1013
Hpy188III TCNNGA 8 cut(s) 110, 143, 464, 487, 539, 666, 753, 871
Hpy8I GTNNAC 3 cut(s) 249, 503, 717
Hpy99I CGWCG 3 cut(s) 29, 545, 829
HpyAV CCTTC 2 cut(s) 600, 871
HpyCH4III ACNGT 2 cut(s) 314, 745
HpyCH4IV ACGT 1 cut(s) 824
HpyCH4V TGCA 2 cut(s) 200, 842
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 4 cut(s) 374, 732, 780, 912
HpySE526I ACGT 1 cut(s) 824
Hsp92I GRCGYC 2 cut(s) 67, 824
Hsp92II CATG 1 cut(s) 790
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 4 cut(s) 101, 668, 702, 873
LmnI GCTCC 1 cut(s) 66
Lsp1109I GCAGC 2 cut(s) 826, 854
LweI GCATC 3 cut(s) 268, 643, 817
MaeI CTAG 4 cut(s) 162, 393, 614, 953
MaeII ACGT 1 cut(s) 824
MalI GATC 4 cut(s) 103, 670, 704, 875
MboI GATC 4 cut(s) 101, 668, 702, 873
MboII GAAGA 5 cut(s) 332, 408, 570, 610, 712
MflI RGATCY 1 cut(s) 873
MhlI GDGCHC 4 cut(s) 63, 293, 733, 740
MlsI TGGCCA 1 cut(s) 574
MluCI AATT 3 cut(s) 146, 155, 404
MluNI TGGCCA 1 cut(s) 574
MlyI GAGTC 3 cut(s) 539, 602, 931
MmeI TCCRAC 2 cut(s) 741, 807
Mox20I TGGCCA 1 cut(s) 574
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 602
MscI TGGCCA 1 cut(s) 574
MslI CAYNNNNRTG 1 cut(s) 244
Msp20I TGGCCA 1 cut(s) 574
MspI CCGG 7 cut(s) 21, 64, 440, 482, 878, 892, 917
MspR9I CCNGG 3 cut(s) 57, 440, 878
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 17
NaeI GCCGGC 1 cut(s) 22
NciI CCSGG 2 cut(s) 440, 878
NdeI CATATG 1 cut(s) 364
NdeII GATC 4 cut(s) 101, 668, 702, 873
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 790
NlaIV GGNNCC 1 cut(s) 62
NmeAIII GCCGAG 1 cut(s) 607
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 94
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 934
PinAI ACCGGT 1 cut(s) 891
PkrI GCNGC 3 cut(s) 222, 841, 844
PleI GAGTC 3 cut(s) 539, 602, 930
PpsI GAGTC 3 cut(s) 539, 602, 930
PpuMI RGGWCCY 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 740
Psp5II RGGWCCY 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 3 cut(s) 179, 972, 992
PspPPI RGGWCCY 1 cut(s) 179
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 1 cut(s) 873
RsaI GTAC 2 cut(s) 76, 785
RsaNI GTAC 2 cut(s) 75, 784
RseI CAYNNNNRTG 1 cut(s) 244
SacI GAGCTC 1 cut(s) 740
SatI GCNGC 3 cut(s) 221, 840, 843
Sau3AI GATC 4 cut(s) 101, 668, 702, 873
Sau96I GGNCC 3 cut(s) 179, 972, 992
SchI GAGTC 3 cut(s) 539, 602, 931
ScrFI CCNGG 3 cut(s) 57, 440, 878
SduI GDGCHC 4 cut(s) 63, 293, 733, 740
SfaNI GCATC 3 cut(s) 268, 643, 817
SfcI CTRYAG 1 cut(s) 840
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 3 cut(s) 146, 155, 404
SsiI CCGC 5 cut(s) 221, 419, 435, 820, 975
SspI AATATT 2 cut(s) 270, 709
SspMI CTAG 4 cut(s) 162, 393, 614, 953
SstI GAGCTC 1 cut(s) 740
StyD4I CCNGG 3 cut(s) 55, 438, 876
TaaI ACNGT 2 cut(s) 314, 745
TaiI ACGT 1 cut(s) 827
TaqI TCGA 7 cut(s) 88, 109, 540, 606, 639, 665, 848
TasI AATT 3 cut(s) 146, 155, 404
TatI WGTACW 1 cut(s) 783
TauI GCSGC 1 cut(s) 223
TfiI GAWTC 1 cut(s) 934
TseI GCWGC 2 cut(s) 839, 842
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 2 cut(s) 146, 155
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 4 cut(s) 162, 393, 614, 953
ZraI GACGTC 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.