Rh5BG304700

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018250
Physical Location & Seq
Forward (+)
39501341 .. 39501877
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG304700.1

Sequence Viewer

Length: 537 bp
ATGGCCATCTTCAACAACTCCTTTCATAAAGAACTTCAACTCTCTAGCAGTTCATCTCGAAATCGAGAGAATCAAAGACTATGTACCGTTGACGGAAGTGGTCTGTATCAAAACTCAACTCGGCGAAGTTTCGATAGTTTCTCTGATGCTCTTGGGTCGAGATCAAAGTGCAGCGACATTGATTCTGGCATGAAGATCAATATCAGGTCGCCGAGTTTCAATGCTCAAAGCTCGACTGCGAGTTCGAGAAAGTTAGGGGTGGAGGTTCTGTCGAAGTACATGAGTGATATTCAGATGAGGAATCGTTGGAGACGGACGTTGGTGACGGCGGCTTCAACCTCCTTCACCTTCCTCTTCCAGATCCCAGCCATCGCCAACCTGTTCAAGTCCATATTGCCTCAGTCGGAGAGTCCGAACCTGATTCTCTCCGACGCCTCGTTAGACAACCTGGAAGGGTGGCCCGCTTCTGATAAAGCTGGCCTGGGACACCATCGCCTCCAGCGAAGGCCCGAACAAGGCGCTGGACTATGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

178

Amino Acids

19.77

Weight (kDa)

9.94

Isoelectric Point (pI)

78.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 329, 462
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 432
AfaI GTAC 2 cut(s) 85, 278
AfiI CCNNNNNNNGG 1 cut(s) 515
AgsI TTSAA 5 cut(s) 13, 38, 220, 336, 385
AjnI CCWGG 2 cut(s) 447, 480
AluBI AGCT 2 cut(s) 231, 476
AluI AGCT 2 cut(s) 231, 476
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 355
AoxI GGCC 4 cut(s) 3, 458, 478, 506
ApeKI GCWGC 1 cut(s) 171
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 459, 507
AsuHPI GGTGA 2 cut(s) 334, 337
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 1 cut(s) 183
BccI CCATC 3 cut(s) 14, 377, 498
BceAI ACGGC 2 cut(s) 342, 516
BciT130I CCWGG 2 cut(s) 449, 482
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 522
BisI GCNGC 2 cut(s) 172, 330
BlsI GCNGC 2 cut(s) 173, 331
Bme1390I CCNGG 2 cut(s) 449, 482
BmgT120I GGNCC 2 cut(s) 459, 507
BmrFI CCNGG 2 cut(s) 449, 482
BmsI GCATC 1 cut(s) 136
BpmI CTGGAG 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 481
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 2 cut(s) 449, 482
BseDI CCNNGG 1 cut(s) 481
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMII CTCAG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 183
BseYI CCCAGC 1 cut(s) 364
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 4 cut(s) 5, 460, 480, 508
BslFI GGGAC 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 1 cut(s) 304
BsmBI CGTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 498
BsnI GGCC 4 cut(s) 5, 460, 480, 508
Bsp143I GATC 3 cut(s) 161, 195, 360
BspACI CCGC 2 cut(s) 329, 462
BspANI GGCC 4 cut(s) 5, 460, 480, 508
BspCNI CTCAG 1 cut(s) 412
BspPI GGATC 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 3 cut(s) 161, 195, 360
BssNI GRCGYC 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 449, 482
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 462, 478
BstDEI CTNAG 1 cut(s) 399
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 3 cut(s) 164, 198, 363
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 3 cut(s) 161, 195, 360
BstNI CCWGG 2 cut(s) 449, 482
BstSCI CCNGG 2 cut(s) 447, 480
BstV1I GCAGC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BsuRI GGCC 4 cut(s) 5, 460, 480, 508
BtgZI GCGATG 2 cut(s) 355, 476
Cac8I GCNNGC 2 cut(s) 462, 478
CfoI GCGC 1 cut(s) 521
Cfr13I GGNCC 2 cut(s) 459, 507
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 84, 277
CviAII CATG 2 cut(s) 190, 280
CviJI RGCY 8 cut(s) 5, 231, 332, 368, 460, 476, 480, 508
CviKI_1 RGCY 8 cut(s) 5, 231, 332, 368, 460, 476, 480, 508
CviQI GTAC 2 cut(s) 84, 277
DdeI CTNAG 1 cut(s) 399
DpnI GATC 3 cut(s) 163, 197, 362
DpnII GATC 3 cut(s) 161, 195, 360
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 447, 480
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 2 cut(s) 193, 283
FaiI YATR 6 cut(s) 27, 82, 191, 281, 392, 529
FaqI GGGAC 1 cut(s) 498
FatI CATG 2 cut(s) 189, 279
FauI CCCGC 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 172, 330
Fsp4HI GCNGC 2 cut(s) 172, 330
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 520
GluI GCNGC 2 cut(s) 172, 330
GsaI CCCAGC 1 cut(s) 368
GsuI CTGGAG 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 4 cut(s) 5, 460, 480, 508
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 521
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 2 cut(s) 193, 283
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 5 cut(s) 70, 182, 301, 409, 421
HphI GGTGA 2 cut(s) 334, 337
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 6 cut(s) 145, 294, 406, 414, 430, 469
Hpy188III TCNNGA 5 cut(s) 57, 65, 159, 246, 358
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 4 cut(s) 352, 358, 446, 498
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 171
HpyF3I CTNAG 1 cut(s) 399
HpySE526I ACGT 1 cut(s) 317
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 2 cut(s) 193, 283
HspAI GCGC 1 cut(s) 519
Kzo9I GATC 3 cut(s) 161, 195, 360
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 136
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 1 cut(s) 322
MalI GATC 3 cut(s) 163, 197, 362
MboI GATC 3 cut(s) 161, 195, 360
MboII GAAGA 2 cut(s) 205, 346
MflI RGATCY 1 cut(s) 360
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 3 cut(s) 287, 384, 453
MnlI CCTC 7 cut(s) 256, 291, 349, 362, 408, 445, 506
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 449, 482
MvaI CCWGG 2 cut(s) 449, 482
NdeII GATC 3 cut(s) 161, 195, 360
NlaIII CATG 2 cut(s) 193, 283
NmeAIII GCCGAG 2 cut(s) 100, 237
NmuCI GTSAC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 2 cut(s) 410, 499
PfeI GAWTC 4 cut(s) 70, 182, 301, 421
PkrI GCNGC 2 cut(s) 173, 331
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 447, 480
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 2 cut(s) 447, 480
PspPI GGNCC 2 cut(s) 459, 507
PsuI RGATCY 1 cut(s) 360
RsaI GTAC 2 cut(s) 85, 278
RsaNI GTAC 2 cut(s) 84, 277
SatI GCNGC 2 cut(s) 172, 330
Sau3AI GATC 3 cut(s) 161, 195, 360
Sau96I GGNCC 2 cut(s) 459, 507
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 2 cut(s) 449, 482
SfaNI GCATC 1 cut(s) 136
SsiI CCGC 2 cut(s) 329, 462
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 447, 480
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 320
TaqI TCGA 7 cut(s) 58, 64, 132, 158, 233, 245, 272
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 4 cut(s) 70, 182, 301, 421
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 14, 42, 206
TspGWI ACGGA 2 cut(s) 108, 328
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.