Rh6AG327600

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018250
Physical Location & Seq
Reverse (-)
52977735 .. 52980745
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG327600.1

Sequence Viewer

Length: 666 bp
ATGGTCGATCTCAGACGGAAAAAAAAATTGCAGAGGGATCCGGCCTTCGTCTCCGACGAAGAATCCGGAGTTTTCTGGAGGAGAGTTAGAGTTCCGGCTGGGCACCCAAGTCAAAAGCCGGCGCCGGTGTGGATCTGTGACTCCGAGATCGCGTACCAGGTCTTCAAACAGCTTCGTCGTCGCTCCACTCCGGCAGCCGCGAACCTACCAAACCTCCGACTCCGGCGTTTCTCTTCGTTCGACTCCTGCTACCACGTAGCTTCAGATCGCTTCCTTGCCGCCGACGAGCCGACGAGGATCCGAACTGTGCAGGCAAAATGGACCCAGCCCTGCGACATCAGTTCTGGCACGAAGATCAATATTGGGTGGCCGAGTTTCGGGGCTCAGAGCTCGACGGTTAGTTCAAGAAAGTTAGAGGTGGAGGCTCTGTCTAAGTACATGAGTGATATTCAGACGAGGCATCATTGGAGGCGGACGTCGGAGACGCAGGCTGCAGCTTCGACCTCCCTCACCTTCAGCTTCCAGATCCCGGCCATCGCCAACCGGTTCAAGTCCATATCGCCTCAGCCGGAGAGTCCGAACTTGAATCTTGCCCTCGCCTCGCTAGACAACCCAGGAAGGTGGCCCGCTTCTGATAAAGCTGGCCCGAGACACCATCACCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

221

Amino Acids

24.98

Weight (kDa)

10.35

Isoelectric Point (pI)

74.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 479
AccB1I GGYRCC 2 cut(s) 102, 121
AccII CGCG 2 cut(s) 152, 200
AccIII TCCGGA 1 cut(s) 65
AciI CCGC 4 cut(s) 198, 279, 472, 627
AclWI GGATC 6 cut(s) 32, 45, 140, 292, 305, 520
AcoI YGGCCR 2 cut(s) 368, 531
AcuI CTGAAG 2 cut(s) 246, 499
AcyI GRCGYC 2 cut(s) 122, 476
AfaI GTAC 2 cut(s) 155, 437
AfiI CCNNNNNNNGG 2 cut(s) 377, 529
AgeI ACCGGT 1 cut(s) 543
AgsI TTSAA 4 cut(s) 166, 405, 550, 586
AjnI CCWGG 2 cut(s) 156, 613
AluBI AGCT 6 cut(s) 172, 260, 390, 497, 519, 641
AluI AGCT 6 cut(s) 172, 260, 390, 497, 519, 641
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 3 cut(s) 55, 476, 643
AlwI GGATC 6 cut(s) 32, 45, 140, 292, 305, 520
Ama87I CYCGRG 1 cut(s) 646
Aor13HI TCCGGA 1 cut(s) 65
AoxI GGCC 5 cut(s) 42, 368, 531, 623, 643
ApeKI GCWGC 3 cut(s) 194, 491, 494
AsiGI ACCGGT 1 cut(s) 543
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 3 cut(s) 321, 624, 644
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 2 cut(s) 502, 650
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 105
BamHI GGATCC 2 cut(s) 37, 297
BanI GGYRCC 2 cut(s) 102, 121
BanII GRGCYC 2 cut(s) 385, 392
BarI GAAGNNNNNNTAC 2 cut(s) 146, 178
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 3 cut(s) 206, 478, 506
BccI CCATC 2 cut(s) 542, 663
BciT130I CCWGG 2 cut(s) 158, 615
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 3 cut(s) 55, 476, 643
BfaI CTAG 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 492
BfoI RGCGCY 1 cut(s) 125
BisI GCNGC 5 cut(s) 195, 198, 279, 492, 495
BlsI GCNGC 5 cut(s) 196, 199, 280, 493, 496
Bme1390I CCNGG 3 cut(s) 158, 530, 615
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 3 cut(s) 321, 624, 644
BmiI GGNNCC 5 cut(s) 39, 104, 123, 299, 323
BmrFI CCNGG 3 cut(s) 158, 530, 615
BmsI GCATC 1 cut(s) 469
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 564
BpuMI CCSGG 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 2 cut(s) 122, 476
BsaJI CCNNGG 1 cut(s) 613
BsaWI WCCGGW 2 cut(s) 65, 543
BsaXI ACNNNNNCTCC 4 cut(s) 198, 228, 413, 443
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 529
Bse118I RCCGGY 3 cut(s) 118, 124, 543
BseAI TCCGGA 1 cut(s) 65
BseBI CCWGG 2 cut(s) 158, 615
BseDI CCNNGG 1 cut(s) 613
BseLI CCNNNNNNNGG 2 cut(s) 377, 529
BseMII CTCAG 3 cut(s) 25, 398, 578
BseRI GAGGAG 1 cut(s) 94
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 3 cut(s) 206, 478, 506
BseYI CCCAGC 2 cut(s) 98, 324
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 2 cut(s) 152, 200
BshFI GGCC 5 cut(s) 44, 370, 533, 625, 645
BshNI GGYRCC 2 cut(s) 102, 121
BshTI ACCGGT 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 646
BslI CCNNNNNNNGG 2 cut(s) 377, 529
BsmAI GTCTC 3 cut(s) 55, 476, 643
BsmBI CGTCTC 2 cut(s) 55, 476
BsnI GGCC 5 cut(s) 44, 370, 533, 625, 645
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 3 cut(s) 105, 385, 392
Bsp13I TCCGGA 1 cut(s) 65
Bsp143I GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
BspACI CCGC 4 cut(s) 198, 279, 472, 627
BspANI GGCC 5 cut(s) 44, 370, 533, 625, 645
BspCNI CTCAG 3 cut(s) 24, 397, 577
BspEI TCCGGA 1 cut(s) 65
BspFNI CGCG 2 cut(s) 152, 200
BspLI GGNNCC 5 cut(s) 39, 104, 123, 299, 323
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 6 cut(s) 32, 45, 140, 292, 305, 520
BspT107I GGYRCC 2 cut(s) 102, 121
BsrFI RCCGGY 3 cut(s) 118, 124, 543
BssAI RCCGGY 3 cut(s) 118, 124, 543
BssECI CCNNGG 1 cut(s) 613
BssMI GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
BssNI GRCGYC 2 cut(s) 122, 476
Bst2UI CCWGG 2 cut(s) 158, 615
Bst4CI ACNGT 2 cut(s) 307, 397
Bst6I CTCTTC 1 cut(s) 238
BstACI GRCGYC 2 cut(s) 122, 476
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 5 cut(s) 120, 312, 489, 627, 643
BstDEI CTNAG 4 cut(s) 11, 384, 432, 564
BstFNI CGCG 2 cut(s) 152, 200
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 8 cut(s) 10, 40, 135, 150, 268, 300, 357, 528
BstMAI GTCTC 3 cut(s) 55, 476, 643
BstMBI GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
BstNI CCWGG 2 cut(s) 158, 615
BstSCI CCNGG 3 cut(s) 156, 528, 613
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 2 cut(s) 152, 200
BstV1I GCAGC 3 cut(s) 206, 478, 506
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 4 cut(s) 37, 132, 297, 525
BstXI CCANNNNNNTGG 1 cut(s) 621
BstYI RGATCY 4 cut(s) 37, 132, 297, 525
BsuRI GGCC 5 cut(s) 44, 370, 533, 625, 645
BtgZI GCGATG 1 cut(s) 520
Cac8I GCNNGC 5 cut(s) 120, 312, 489, 627, 643
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 3 cut(s) 118, 124, 543
Cfr13I GGNCC 3 cut(s) 321, 624, 644
CseI GACGC 1 cut(s) 493
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 2 cut(s) 154, 436
CspAI ACCGGT 1 cut(s) 543
CviAII CATG 1 cut(s) 439
CviQI GTAC 2 cut(s) 154, 436
DdeI CTNAG 4 cut(s) 11, 384, 432, 564
DinI GGCGCC 1 cut(s) 123
DpnI GATC 8 cut(s) 9, 39, 134, 149, 267, 299, 356, 527
DpnII GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
EaeI YGGCCR 2 cut(s) 368, 531
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
EciI GGCGGA 1 cut(s) 487
Ecl136II GAGCTC 1 cut(s) 390
Eco24I GRGCYC 2 cut(s) 385, 392
Eco47I GGWCC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 2 cut(s) 246, 499
Eco88I CYCGRG 1 cut(s) 646
EcoICRI GAGCTC 1 cut(s) 390
EcoRII CCWGG 2 cut(s) 156, 613
EcoT38I GRGCYC 2 cut(s) 385, 392
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
Esp3I CGTCTC 2 cut(s) 55, 476
FaeI CATG 1 cut(s) 442
FaiI YATR 2 cut(s) 440, 557
FatI CATG 1 cut(s) 438
FauI CCCGC 1 cut(s) 634
Fnu4HI GCNGC 5 cut(s) 195, 198, 279, 492, 495
FriOI GRGCYC 2 cut(s) 385, 392
Fsp4HI GCNGC 5 cut(s) 195, 198, 279, 492, 495
FspBI CTAG 1 cut(s) 605
GlaI GCGC 1 cut(s) 123
GluI GCNGC 5 cut(s) 195, 198, 279, 492, 495
GsaI CCCAGC 2 cut(s) 102, 328
GsuI CTGGAG 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 5 cut(s) 44, 370, 533, 625, 645
HgaI GACGC 1 cut(s) 493
HhaI GCGC 1 cut(s) 124
Hin1I GRCGYC 2 cut(s) 122, 476
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 6 cut(s) 62, 140, 219, 242, 574, 586
HphI GGTGA 2 cut(s) 502, 650
Hpy188III TCNNGA 4 cut(s) 66, 76, 405, 523
Hpy99I CGWCG 7 cut(s) 59, 180, 183, 287, 295, 397, 481
HpyAV CCTTC 3 cut(s) 55, 523, 612
HpyCH4III ACNGT 2 cut(s) 307, 397
HpyCH4IV ACGT 2 cut(s) 255, 476
HpyCH4V TGCA 3 cut(s) 31, 310, 494
HpyF3I CTNAG 4 cut(s) 11, 384, 432, 564
HpySE526I ACGT 2 cut(s) 255, 476
Hsp92I GRCGYC 2 cut(s) 122, 476
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 1 cut(s) 122
KasI GGCGCC 1 cut(s) 121
Kpn2I TCCGGA 1 cut(s) 65
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Kzo9I GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 3 cut(s) 206, 478, 506
LweI GCATC 1 cut(s) 469
MabI ACCWGGT 1 cut(s) 156
MaeI CTAG 1 cut(s) 605
MaeII ACGT 2 cut(s) 255, 476
MaeIII GTNAC 1 cut(s) 137
MalI GATC 8 cut(s) 9, 39, 134, 149, 267, 299, 356, 527
MboI GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
MboII GAAGA 4 cut(s) 71, 154, 225, 364
MflI RGATCY 4 cut(s) 37, 132, 297, 525
MhlI GDGCHC 3 cut(s) 105, 385, 392
MluCI AATT 1 cut(s) 26
Mly113I GGCGCC 1 cut(s) 122
MlyI GAGTC 4 cut(s) 134, 213, 236, 583
MmeI TCCRAC 3 cut(s) 78, 241, 459
MroI TCCGGA 1 cut(s) 65
MroNI GCCGGC 1 cut(s) 118
MspR9I CCNGG 3 cut(s) 158, 530, 615
MvaI CCWGG 2 cut(s) 158, 615
MvnI CGCG 2 cut(s) 152, 200
NaeI GCCGGC 1 cut(s) 120
NarI GGCGCC 1 cut(s) 122
NciI CCSGG 1 cut(s) 530
NdeII GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 5 cut(s) 39, 104, 123, 299, 323
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdiI GCCGGC 1 cut(s) 120
PfeI GAWTC 2 cut(s) 62, 586
PinAI ACCGGT 1 cut(s) 543
PkrI GCNGC 5 cut(s) 196, 199, 280, 493, 496
PleI GAGTC 4 cut(s) 134, 213, 236, 582
PluTI GGCGCC 1 cut(s) 125
PpsI GAGTC 4 cut(s) 134, 213, 236, 582
Ppu21I YACGTR 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 156, 613
PspFI CCCAGC 2 cut(s) 98, 324
PspGI CCWGG 2 cut(s) 156, 613
PspN4I GGNNCC 5 cut(s) 39, 104, 123, 299, 323
PspPI GGNCC 3 cut(s) 321, 624, 644
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 4 cut(s) 37, 132, 297, 525
RsaI GTAC 2 cut(s) 155, 437
RsaNI GTAC 2 cut(s) 154, 436
SacI GAGCTC 1 cut(s) 392
SatI GCNGC 5 cut(s) 195, 198, 279, 492, 495
Sau3AI GATC 8 cut(s) 7, 37, 132, 147, 265, 297, 354, 525
Sau96I GGNCC 3 cut(s) 321, 624, 644
SchI GAGTC 4 cut(s) 134, 213, 236, 583
ScrFI CCNGG 3 cut(s) 158, 530, 615
SduI GDGCHC 3 cut(s) 105, 385, 392
SexAI ACCWGGT 1 cut(s) 156
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 492
SfoI GGCGCC 1 cut(s) 123
SgrAI CRCCGGYG 1 cut(s) 124
SinI GGWCC 1 cut(s) 321
Sse9I AATT 1 cut(s) 26
SsiI CCGC 4 cut(s) 198, 279, 472, 627
SspDI GGCGCC 1 cut(s) 121
SspI AATATT 1 cut(s) 361
SspMI CTAG 1 cut(s) 605
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 156, 528, 613
TaaI ACNGT 2 cut(s) 307, 397
TaiI ACGT 2 cut(s) 258, 479
TaqI TCGA 4 cut(s) 6, 240, 392, 500
TasI AATT 1 cut(s) 26
TatI WGTACW 1 cut(s) 435
TauI GCSGC 2 cut(s) 200, 281
TfiI GAWTC 2 cut(s) 62, 586
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 3 cut(s) 194, 491, 494
Tsp45I GTSAC 1 cut(s) 137
TspGWI ACGGA 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 321
XspI CTAG 1 cut(s) 605
ZraI GACGTC 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.