Rroxscaffold_6G00412340

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0018250
Physical Location & Seq
Forward (+)
34956488 .. 34957062
575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412340.1

Sequence Viewer

Length: 489 bp
ATGGATCGGTCTCACAAACCCGGAAGACGCAAAACTCATCTCGACGATTCAACCTCCATCCGAGGAGGACTATGGCCATCTTCAACAATTCCTTCCATGAACAACTTCGACTCTCTAGCAGTCCATCTCGAAATCGAGAGAATCAAAGACCGTATTGACGTCGGTTCGGCACGAAGATCAATAATAGGTCGTCGAGTTTCGGGGTTAAGAGCTCGACGGCGAGTTCGAGAAAGTTGGGGGGTGGAGGTTCTGTCCAAGTACATGAGTGATATTCGGACAAGGCATGATTGGAGACCGATGTCGGTGACGGCGGATTCGACCTCCCTCACTTTCCGCCTCCGGATCCCGGTCATCGCCAACCGGTTCAAGCGGCCCGAGACACCATCGCCTCCGGCGAAGGCCCTAGCGGGCGCGGACTATGCCGTCGGGGAGGCTAGGTCGTTCGAGCGGTATGCCGTCGACGACGAGCCCGGCCGGACCTGGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

162

Amino Acids

18.44

Weight (kDa)

10.93

Isoelectric Point (pI)

70.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AatII GACGTC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 448
AccI GTMKAC 1 cut(s) 459
AccII CGCG 1 cut(s) 413
AccIII TCCGGA 1 cut(s) 339
AciI CCGC 6 cut(s) 311, 334, 370, 407, 413, 448
AclWI GGATC 3 cut(s) 12, 337, 350
AcoI YGGCCR 2 cut(s) 74, 472
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 346
AgeI ACCGGT 1 cut(s) 360
AgsI TTSAA 3 cut(s) 51, 84, 367
AjnI CCWGG 1 cut(s) 479
AluBI AGCT 1 cut(s) 212
AluI AGCT 1 cut(s) 212
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 3 cut(s) 15, 286, 371
AlwI GGATC 3 cut(s) 12, 337, 350
Ama87I CYCGRG 1 cut(s) 374
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 4 cut(s) 74, 371, 399, 472
ArsI GACNNNNNNTTYG 4 cut(s) 207, 239, 298, 330
AsiGI ACCGGT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 104
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 3 cut(s) 372, 400, 477
AsuC2I CCSGG 3 cut(s) 21, 347, 471
AsuHPI GGTGA 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 477
BalI TGGCCA 1 cut(s) 76
BamHI GGATCC 1 cut(s) 342
BanII GRGCYC 2 cut(s) 214, 471
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 214
BccI CCATC 4 cut(s) 65, 85, 132, 391
BceAI ACGGC 4 cut(s) 233, 324, 407, 440
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 1 cut(s) 481
BcnI CCSGG 3 cut(s) 21, 347, 471
BcoDI GTCTC 3 cut(s) 15, 286, 371
BfaI CTAG 3 cut(s) 116, 404, 435
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
Bme1390I CCNGG 4 cut(s) 21, 347, 471, 481
Bme18I GGWCC 1 cut(s) 477
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 3 cut(s) 372, 400, 477
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 4 cut(s) 21, 347, 471, 481
BoxI GACNNNNGTC 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 31
BpuMI CCSGG 3 cut(s) 21, 347, 471
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 2 cut(s) 15, 286
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 2 cut(s) 339, 360
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse118I RCCGGY 1 cut(s) 360
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 346
BseRI GAGGAG 1 cut(s) 78
BseX3I CGGCCG 1 cut(s) 472
Bsh1236I CGCG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 4 cut(s) 76, 373, 401, 474
BshTI ACCGGT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 7 cut(s) 21, 340, 347, 361, 392, 471, 475
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 3 cut(s) 15, 286, 371
BsnI GGCC 4 cut(s) 76, 373, 401, 474
Bso31I GGTCTC 2 cut(s) 15, 286
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 214, 471
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 3 cut(s) 4, 176, 342
BspACI CCGC 6 cut(s) 311, 334, 370, 407, 413, 448
BspANI GGCC 4 cut(s) 76, 373, 401, 474
BspEI TCCGGA 1 cut(s) 339
BspFNI CGCG 1 cut(s) 413
BspHI TCATGA 1 cut(s) 485
BspLI GGNNCC 1 cut(s) 344
BspPI GGATC 3 cut(s) 12, 337, 350
BspTNI GGTCTC 2 cut(s) 15, 286
BsrBI CCGCTC 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 360
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 3 cut(s) 4, 176, 342
BssNI GRCGYC 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 409
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 3 cut(s) 7, 179, 345
BstMAI GTCTC 3 cut(s) 15, 286, 371
BstMBI GATC 3 cut(s) 4, 176, 342
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 481
BstPAI GACNNNNGTC 1 cut(s) 298
BstSCI CCNGG 4 cut(s) 19, 345, 469, 479
BstUI CGCG 1 cut(s) 413
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BstZI CGGCCG 1 cut(s) 472
BsuRI GGCC 4 cut(s) 76, 373, 401, 474
BtgZI GCGATG 2 cut(s) 337, 369
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 409
CciI TCATGA 1 cut(s) 485
CfoI GCGC 1 cut(s) 413
Cfr10I RCCGGY 1 cut(s) 360
Cfr13I GGNCC 3 cut(s) 372, 400, 477
CseI GACGC 1 cut(s) 36
CsiI ACCWGGT 1 cut(s) 479
Csp6I GTAC 1 cut(s) 259
CspAI ACCGGT 1 cut(s) 360
CviAII CATG 4 cut(s) 97, 262, 284, 486
CviJI RGCY 7 cut(s) 76, 212, 373, 401, 434, 469, 474
CviKI_1 RGCY 7 cut(s) 76, 212, 373, 401, 434, 469, 474
CviQI GTAC 1 cut(s) 259
DpnI GATC 3 cut(s) 6, 178, 344
DpnII GATC 3 cut(s) 4, 176, 342
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
EaeI YGGCCR 2 cut(s) 74, 472
EagI CGGCCG 1 cut(s) 472
EciI GGCGGA 2 cut(s) 323, 326
Ecl136II GAGCTC 1 cut(s) 212
EclXI CGGCCG 1 cut(s) 472
Eco24I GRGCYC 2 cut(s) 214, 471
Eco31I GGTCTC 2 cut(s) 15, 286
Eco47I GGWCC 1 cut(s) 477
Eco52I CGGCCG 1 cut(s) 472
Eco53kI GAGCTC 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 479
EcoT38I GRGCYC 2 cut(s) 214, 471
FaeI CATG 4 cut(s) 100, 265, 287, 489
FaiI YATR 7 cut(s) 73, 98, 263, 285, 420, 453, 487
FatI CATG 4 cut(s) 96, 261, 283, 485
FauI CCCGC 1 cut(s) 400
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 2 cut(s) 214, 471
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 3 cut(s) 116, 404, 435
GlaI GCGC 1 cut(s) 412
GluI GCNGC 1 cut(s) 371
HaeIII GGCC 4 cut(s) 76, 373, 401, 474
HapII CCGG 7 cut(s) 21, 340, 347, 361, 392, 471, 475
HgaI GACGC 1 cut(s) 36
HhaI GCGC 1 cut(s) 413
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 100, 265, 287, 489
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 4 cut(s) 47, 110, 141, 314
HpaII CCGG 7 cut(s) 21, 340, 347, 361, 392, 471, 475
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 2 cut(s) 62, 276
Hpy188III TCNNGA 6 cut(s) 41, 128, 136, 227, 340, 486
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 8 cut(s) 47, 164, 195, 219, 428, 461, 464, 467
HpyAV CCTTC 2 cut(s) 102, 391
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 100, 265, 287, 489
HspAI GCGC 1 cut(s) 411
Kpn2I TCCGGA 1 cut(s) 339
Kzo9I GATC 3 cut(s) 4, 176, 342
LpnPI CCDG 7 cut(s) 34, 353, 360, 374, 405, 466, 484
MabI ACCWGGT 1 cut(s) 479
MaeI CTAG 3 cut(s) 116, 404, 435
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 304
MalI GATC 3 cut(s) 6, 178, 344
MbiI CCGCTC 1 cut(s) 448
MboI GATC 3 cut(s) 4, 176, 342
MboII GAAGA 3 cut(s) 36, 72, 186
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 2 cut(s) 214, 471
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 76
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 9 cut(s) 56, 59, 64, 238, 331, 335, 347, 399, 424
Mox20I TGGCCA 1 cut(s) 76
MroI TCCGGA 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 104
MscI TGGCCA 1 cut(s) 76
MseI TTAA 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 76
MspI CCGG 7 cut(s) 21, 340, 347, 361, 392, 471, 475
MspR9I CCNGG 4 cut(s) 21, 347, 471, 481
MvaI CCWGG 1 cut(s) 481
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 419
NciI CCSGG 3 cut(s) 21, 347, 471
NdeII GATC 3 cut(s) 4, 176, 342
NlaIII CATG 4 cut(s) 100, 265, 287, 489
NlaIV GGNNCC 1 cut(s) 344
NmuCI GTSAC 1 cut(s) 304
PagI TCATGA 1 cut(s) 485
PcsI WCGNNNNNNNCGW 3 cut(s) 223, 314, 392
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 3 cut(s) 47, 141, 314
PflFI GACNNNGTC 1 cut(s) 481
PinAI ACCGGT 1 cut(s) 360
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PshAI GACNNNNGTC 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 479
PspGI CCWGG 1 cut(s) 479
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 3 cut(s) 372, 400, 477
PsuI RGATCY 1 cut(s) 342
PsyI GACNNNGTC 1 cut(s) 481
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SacI GAGCTC 1 cut(s) 214
SalI GTCGAC 1 cut(s) 458
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 3 cut(s) 4, 176, 342
Sau96I GGNCC 3 cut(s) 372, 400, 477
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 4 cut(s) 21, 347, 471, 481
SduI GDGCHC 2 cut(s) 214, 471
SetI ASST 8 cut(s) 56, 162, 190, 214, 249, 323, 440, 482
SexAI ACCWGGT 1 cut(s) 479
SgrDI CGTCGACG 1 cut(s) 458
SinI GGWCC 1 cut(s) 477
Sse9I AATT 1 cut(s) 87
SsiI CCGC 6 cut(s) 311, 334, 370, 407, 413, 448
SspMI CTAG 3 cut(s) 116, 404, 435
SstI GAGCTC 1 cut(s) 214
StyD4I CCNGG 4 cut(s) 19, 345, 469, 479
TaaI ACNGT 1 cut(s) 152
TaiI ACGT 1 cut(s) 162
TaqII GACCGA 1 cut(s) 310
TasI AATT 1 cut(s) 87
TatI WGTACW 1 cut(s) 258
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 3 cut(s) 47, 141, 314
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TseFI GTSAC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 113
Tth111I GACNNNGTC 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 3 cut(s) 116, 404, 435
ZraI GACGTC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.