Rh5AG223500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018250
Physical Location & Seq
Forward (+)
26585278 .. 26585511
234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG223500.1

Sequence Viewer

Length: 234 bp
ATGAGTGATATTCAGACAAGGCATGATTGGAGACTGATGTCGGCAACGGCGGCTTCAACCCCCCTCACCCTTCGCCTCCAGATCCCAGTCATCGCCAACCGGTTCAAGTCCATATCACCTAAGCCGGAGAGTCAGAATCTGATTCTCATCGGCGCCTCGCTCAACAACCCGGAAGGGTGGCCCGCTTCTGATAAAGCTGGCCCGAGACACCATCGCCTCCAGCGAAGGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

77

Amino Acids

8.65

Weight (kDa)

11.55

Isoelectric Point (pI)

64.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AciI CCGC 2 cut(s) 50, 183
AclWI GGATC 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 153
AgeI ACCGGT 1 cut(s) 99
AgsI TTSAA 2 cut(s) 57, 106
AluBI AGCT 1 cut(s) 197
AluI AGCT 1 cut(s) 197
Alw26I GTCTC 2 cut(s) 25, 199
AlwI GGATC 1 cut(s) 76
AlwNI CAGNNNCTG 1 cut(s) 139
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 3 cut(s) 179, 199, 227
AsiGI ACCGGT 1 cut(s) 99
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 3 cut(s) 180, 200, 228
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 58, 108
AvaI CYCGRG 1 cut(s) 202
BanI GGYRCC 1 cut(s) 152
BccI CCATC 1 cut(s) 219
BceAI ACGGC 1 cut(s) 63
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 25, 199
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 3 cut(s) 180, 200, 228
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 170
BmrI ACTGGG 1 cut(s) 80
BmuI ACTGGG 1 cut(s) 80
BoxI GACNNNNGTC 1 cut(s) 37
BpmI CTGGAG 2 cut(s) 62, 203
Bpu10I CCTNAGC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 99
Bse1I ACTGG 1 cut(s) 86
Bse8I GATNNNNATC 1 cut(s) 146
BseJI GATNNNNATC 1 cut(s) 146
BseNI ACTGG 1 cut(s) 86
BshFI GGCC 3 cut(s) 181, 201, 229
BshNI GGYRCC 1 cut(s) 152
BshTI ACCGGT 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 3 cut(s) 100, 125, 170
BsmAI GTCTC 2 cut(s) 25, 199
BsnI GGCC 3 cut(s) 181, 201, 229
BsoBI CYCGRG 1 cut(s) 202
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 2 cut(s) 50, 183
BspANI GGCC 3 cut(s) 181, 201, 229
BspLI GGNNCC 1 cut(s) 154
BspPI GGATC 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 99
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 99
BssMI GATC 1 cut(s) 81
BssNI GRCGYC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 2 cut(s) 183, 199
BstDEI CTNAG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 25, 199
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstPAI GACNNNNGTC 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 3 cut(s) 181, 201, 229
BtgZI GCGATG 2 cut(s) 76, 197
Cac8I GCNNGC 2 cut(s) 183, 199
CaiI CAGNNNCTG 1 cut(s) 139
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 180, 200, 228
CspAI ACCGGT 1 cut(s) 99
CviAII CATG 1 cut(s) 23
CviJI RGCY 6 cut(s) 53, 124, 181, 197, 201, 229
CviKI_1 RGCY 6 cut(s) 53, 124, 181, 197, 201, 229
DdeI CTNAG 1 cut(s) 120
DinI GGCGCC 1 cut(s) 154
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 228
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaeI CATG 1 cut(s) 26
FaiI YATR 2 cut(s) 24, 113
FatI CATG 1 cut(s) 22
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 51
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 51
GsuI CTGGAG 2 cut(s) 62, 203
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 3 cut(s) 181, 201, 229
HapII CCGG 3 cut(s) 100, 125, 170
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 1 cut(s) 26
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 3 cut(s) 130, 136, 142
HpaII CCGG 3 cut(s) 100, 125, 170
HphI GGTGA 2 cut(s) 58, 108
Hpy188I TCNGA 4 cut(s) 15, 135, 141, 190
Hpy188III TCNNGA 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 80, 167, 219
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 1 cut(s) 26
HspAI GCGC 1 cut(s) 153
KasI GGCGCC 1 cut(s) 152
Kzo9I GATC 1 cut(s) 81
LpnPI CCDG 6 cut(s) 92, 99, 113, 138, 183, 183
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MflI RGATCY 1 cut(s) 81
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 4 cut(s) 74, 86, 166, 227
MspI CCGG 3 cut(s) 100, 125, 170
MspR9I CCNGG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 50
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 170
NdeII GATC 1 cut(s) 81
NlaIII CATG 1 cut(s) 26
NlaIV GGNNCC 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PfeI GAWTC 2 cut(s) 136, 142
PinAI ACCGGT 1 cut(s) 99
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 138
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 138
PshAI GACNNNNGTC 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 3 cut(s) 180, 200, 228
PstNI CAGNNNCTG 1 cut(s) 139
PsuI RGATCY 1 cut(s) 81
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 1 cut(s) 81
Sau96I GGNCC 3 cut(s) 180, 200, 228
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 170
SetI ASST 2 cut(s) 121, 199
SfoI GGCGCC 1 cut(s) 154
SsiI CCGC 2 cut(s) 50, 183
SspDI GGCGCC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 168
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 2 cut(s) 136, 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.