Rmu_ssc0000261.1_g000024

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000261.1
OG0018250
Physical Location & Seq
Reverse (-)
172156 .. 173054
899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000261.1_g000024.1.cds

Sequence Viewer

Length: 753 bp
atggatcagtctcacaaacccagaagacccaaattgactacaaacccgcttcccatccccaccgccccggaaatctccacacttccatctccaaaccaccctctctcgccggtcaagagaaaacccagttcgccgatatctgatacccgaaaacccaaaactcatctcgacgactcaacctccatccgaggaggactatggccatcttcaacaactccttccctgaagaacttcgactctctagcaaactcaactcggcgaagtttcgatagtttctctgatgctctcgggtcgagatcaaagtgtagcgacatcggttctggcacgaagatcaatattgggtggccgagtttcggggctcagagctcgacggttagttcaagaaagttggaggtggaggctctgtctaagtacatgagtgatattcagacgaggcatcattggaggcggacgtcggcgacgcaggctgcagcttcgacctccctcaccttcagcttccagatcccggccatcgccaaccggttcaagtccatatcgcctcagccggagagtctgaacttgaatctcgcccacgccttgctagacaacccagaaaggaaggccaagtcattcgagcggtgtgccgtcgacgacgagcccaacctagacctggtgatgagtctgcatgtgctgagtctaattaaaggggctttctttctcaatctgtggagatgggtgaaggaggaggtccttgctctaagaagatggagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

250

Amino Acids

27.86

Weight (kDa)

10.14

Isoelectric Point (pI)

66.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 616
AccI GTMKAC 1 cut(s) 627
AciI CCGC 4 cut(s) 47, 63, 448, 616
AclWI GGATC 2 cut(s) 12, 496
AcoI YGGCCR 3 cut(s) 200, 344, 507
AcuI CTGAAG 2 cut(s) 245, 475
AcyI GRCGYC 1 cut(s) 452
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 3 cut(s) 353, 505, 649
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 4 cut(s) 210, 381, 526, 562
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 3 cut(s) 366, 473, 495
AluI AGCT 3 cut(s) 366, 473, 495
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 2 cut(s) 12, 496
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 4 cut(s) 200, 344, 507, 600
ApeKI GCWGC 2 cut(s) 467, 470
AsiGI ACCGGT 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 727
AsuC2I CCSGG 2 cut(s) 68, 506
AsuHPI GGTGA 3 cut(s) 478, 664, 727
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 727
BalI TGGCCA 1 cut(s) 202
BanII GRGCYC 3 cut(s) 361, 368, 639
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 454, 482
BccI CCATC 7 cut(s) 62, 94, 191, 211, 518, 705, 738
BceAI ACGGC 1 cut(s) 608
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 1 cut(s) 650
BcnI CCSGG 2 cut(s) 68, 506
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 3 cut(s) 242, 581, 644
BfmI CTRYAG 1 cut(s) 468
BisI GCNGC 2 cut(s) 468, 471
BlsI GCNGC 2 cut(s) 469, 472
Bme1390I CCNGG 3 cut(s) 68, 506, 650
Bme18I GGWCC 1 cut(s) 727
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 727
BmrFI CCNGG 3 cut(s) 68, 506, 650
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 271, 445
BmuI ACTGGG 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 540
BpuMI CCSGG 2 cut(s) 68, 506
BsaHI GRCGYC 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 66, 187
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 389, 419
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 505, 649
Bse118I RCCGGY 2 cut(s) 109, 519
Bse1I ACTGG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 2 cut(s) 66, 187
BseGI GGATG 2 cut(s) 54, 183
BseLI CCNNNNNNNGG 3 cut(s) 353, 505, 649
BseMII CTCAG 3 cut(s) 374, 554, 662
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 2 cut(s) 204, 737
BseXI GCAGC 2 cut(s) 454, 482
BshFI GGCC 4 cut(s) 202, 346, 509, 602
BshTI ACCGGT 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 368
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 5 cut(s) 68, 110, 506, 520, 545
BslI CCNNNNNNNGG 3 cut(s) 353, 505, 649
BsmAI GTCTC 1 cut(s) 15
BsnI GGCC 4 cut(s) 202, 346, 509, 602
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 361, 368, 639
Bsp143I GATC 4 cut(s) 4, 296, 330, 501
BspACI CCGC 4 cut(s) 47, 63, 448, 616
BspANI GGCC 4 cut(s) 202, 346, 509, 602
BspCNI CTCAG 3 cut(s) 373, 553, 663
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 2 cut(s) 12, 496
BsrBI CCGCTC 1 cut(s) 616
BsrFI RCCGGY 2 cut(s) 109, 519
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 2 cut(s) 109, 519
BssECI CCNNGG 2 cut(s) 66, 187
BssMI GATC 4 cut(s) 4, 296, 330, 501
BssNI GRCGYC 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 1 cut(s) 373
BstACI GRCGYC 1 cut(s) 452
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 5 cut(s) 360, 408, 540, 671, 737
BstF5I GGATG 2 cut(s) 54, 183
BstKTI GATC 4 cut(s) 7, 299, 333, 504
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 4 cut(s) 4, 296, 330, 501
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 3 cut(s) 66, 504, 648
BstSFI CTRYAG 1 cut(s) 468
BstV1I GCAGC 2 cut(s) 454, 482
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 501
BstYI RGATCY 1 cut(s) 501
BsuRI GGCC 4 cut(s) 202, 346, 509, 602
BtgZI GCGATG 1 cut(s) 496
BtsCI GGATG 2 cut(s) 54, 183
Cac8I GCNNGC 1 cut(s) 465
Cfr10I RCCGGY 2 cut(s) 109, 519
Cfr13I GGNCC 1 cut(s) 727
CseI GACGC 1 cut(s) 469
CsiI ACCWGGT 1 cut(s) 648
Csp6I GTAC 1 cut(s) 412
CspAI ACCGGT 1 cut(s) 519
CviAII CATG 2 cut(s) 415, 665
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 5 cut(s) 360, 408, 540, 671, 737
DpnI GATC 4 cut(s) 6, 298, 332, 503
DpnII GATC 4 cut(s) 4, 296, 330, 501
EaeI YGGCCR 3 cut(s) 200, 344, 507
EciI GGCGGA 1 cut(s) 463
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 3 cut(s) 361, 368, 639
Eco32I GATATC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 727
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 2 cut(s) 245, 475
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 727
EcoRII CCWGG 1 cut(s) 648
EcoRV GATATC 1 cut(s) 138
EcoT38I GRGCYC 3 cut(s) 361, 368, 639
FaeI CATG 2 cut(s) 418, 668
FaiI YATR 4 cut(s) 199, 416, 533, 666
FatI CATG 2 cut(s) 414, 664
FauI CCCGC 1 cut(s) 54
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 2 cut(s) 468, 471
FokI GGATG 2 cut(s) 41, 170
FriOI GRGCYC 3 cut(s) 361, 368, 639
Fsp4HI GCNGC 2 cut(s) 468, 471
FspBI CTAG 3 cut(s) 242, 581, 644
GluI GCNGC 2 cut(s) 468, 471
HaeIII GGCC 4 cut(s) 202, 346, 509, 602
HapII CCGG 5 cut(s) 68, 110, 506, 520, 545
HgaI GACGC 1 cut(s) 469
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 2 cut(s) 418, 668
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 6 cut(s) 173, 236, 550, 562, 658, 673
HpaII CCGG 5 cut(s) 68, 110, 506, 520, 545
HphI GGTGA 3 cut(s) 478, 664, 727
Hpy166II GTNNAC 1 cut(s) 628
Hpy188I TCNGA 6 cut(s) 142, 188, 280, 363, 429, 555
Hpy188III TCNNGA 5 cut(s) 115, 167, 294, 381, 499
Hpy8I GTNNAC 1 cut(s) 628
Hpy99I CGWCG 7 cut(s) 173, 373, 457, 463, 629, 632, 635
HpyAV CCTTC 4 cut(s) 228, 499, 592, 712
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 2 cut(s) 470, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 5 cut(s) 360, 408, 540, 671, 737
HpySE526I ACGT 1 cut(s) 452
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 2 cut(s) 418, 668
Kzo9I GATC 4 cut(s) 4, 296, 330, 501
Lsp1109I GCAGC 2 cut(s) 454, 482
LweI GCATC 2 cut(s) 271, 445
MabI ACCWGGT 1 cut(s) 648
MaeI CTAG 3 cut(s) 242, 581, 644
MaeII ACGT 1 cut(s) 452
MalI GATC 4 cut(s) 6, 298, 332, 503
MbiI CCGCTC 1 cut(s) 616
MboI GATC 4 cut(s) 4, 296, 330, 501
MboII GAAGA 5 cut(s) 36, 198, 238, 340, 753
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 3 cut(s) 361, 368, 639
MlsI TGGCCA 1 cut(s) 202
MluCI AATT 2 cut(s) 32, 678
MluNI TGGCCA 1 cut(s) 202
MlyI GAGTC 5 cut(s) 167, 230, 559, 667, 682
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 202
MseI TTAA 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 202
MspI CCGG 5 cut(s) 68, 110, 506, 520, 545
MspR9I CCNGG 3 cut(s) 68, 506, 650
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 1 cut(s) 464
NciI CCSGG 2 cut(s) 68, 506
NdeII GATC 4 cut(s) 4, 296, 330, 501
NlaIII CATG 2 cut(s) 418, 668
NmeAIII GCCGAG 2 cut(s) 235, 372
NspI RCATGY 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 562
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 2 cut(s) 469, 472
PleI GAGTC 5 cut(s) 167, 230, 558, 666, 681
PpsI GAGTC 5 cut(s) 167, 230, 558, 666, 681
PpuMI RGGWCCY 1 cut(s) 727
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 727
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspPI GGNCC 1 cut(s) 727
PspPPI RGGWCCY 1 cut(s) 727
PstI CTGCAG 1 cut(s) 472
PsuI RGATCY 1 cut(s) 501
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
SacI GAGCTC 1 cut(s) 368
SalI GTCGAC 1 cut(s) 626
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 2 cut(s) 468, 471
Sau3AI GATC 4 cut(s) 4, 296, 330, 501
Sau96I GGNCC 1 cut(s) 727
SchI GAGTC 5 cut(s) 167, 230, 559, 667, 682
ScrFI CCNGG 3 cut(s) 68, 506, 650
SduI GDGCHC 3 cut(s) 361, 368, 639
SexAI ACCWGGT 1 cut(s) 648
SfaNI GCATC 2 cut(s) 271, 445
SfcI CTRYAG 1 cut(s) 468
SgrDI CGTCGACG 1 cut(s) 626
SinI GGWCC 1 cut(s) 727
Sse9I AATT 2 cut(s) 32, 678
SsiI CCGC 4 cut(s) 47, 63, 448, 616
SspI AATATT 1 cut(s) 337
SspMI CTAG 3 cut(s) 242, 581, 644
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 66, 504, 648
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 455
TaqI TCGA 8 cut(s) 168, 234, 267, 293, 368, 476, 612, 627
TasI AATT 2 cut(s) 32, 678
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TseI GCWGC 2 cut(s) 467, 470
VpaK11BI GGWCC 1 cut(s) 727
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 3 cut(s) 242, 581, 644
ZraI GACGTC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.