Rmu_sc0021525.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021525.1
OG0009408
Physical Location & Seq
Forward (+)
6109 .. 6515
407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021525.1_g000003.1.cds

Sequence Viewer

Length: 312 bp
atgtactctcacaaaggtttgacaactcctcctcatccttcaccggcagtgcctattattcctcggcctattccgacatccgtcagaacaaccacagaacagagccggattcgaagagcagctgcagtttttgctgcgaaatctggtggaggattttcactcaactcgatcttaaaaaggtgtgaaacctgcgaaggcaaaggtgcaattgactgtcagggttgcaaggtacgctttcacgacttcatttcactttcaccctttacgtatacattgtactatggtccgggaccatgtaataatttcccgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.19

Weight (kDa)

9.5

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 269
AfaI GTAC 3 cut(s) 5, 231, 278
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 119, 122, 134
AspS9I GGNCC 2 cut(s) 284, 290
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 33, 249
AsuII TTCGAA 1 cut(s) 112
AvaII GGWCC 2 cut(s) 284, 290
BbvI GCAGC 3 cut(s) 109, 121, 131
BcnI CCSGG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 3 cut(s) 120, 123, 135
BlsI GCNGC 3 cut(s) 121, 124, 136
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 2 cut(s) 284, 290
BmgT120I GGNCC 2 cut(s) 284, 290
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 288
BoxI GACNNNNGTC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 112
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bse118I RCCGGY 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 62
BseGI GGATG 2 cut(s) 34, 77
BseRI GAGGAG 2 cut(s) 18, 21
BseXI GCAGC 3 cut(s) 109, 121, 131
BshFI GGCC 1 cut(s) 67
BsiSI CCGG 3 cut(s) 44, 106, 287
BslFI GGGAC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 1 cut(s) 67
Bsp119I TTCGAA 1 cut(s) 112
Bsp143I GATC 1 cut(s) 168
BspANI GGCC 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 291
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 197
BspQI GCTCTTC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 62
BssMI GATC 1 cut(s) 168
BssNAI GTATAC 1 cut(s) 270
Bst1107I GTATAC 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 131
BstBAI YACGTR 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 112
BstF5I GGATG 2 cut(s) 34, 77
BstKTI GATC 1 cut(s) 171
BstMBI GATC 1 cut(s) 168
BstMWI GCNNNNNNNGC 2 cut(s) 131, 231
BstPAI GACNNNNGTC 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 123
BstSNI TACGTA 1 cut(s) 267
BstV1I GCAGC 3 cut(s) 109, 121, 131
BstZ17I GTATAC 1 cut(s) 270
BsuRI GGCC 1 cut(s) 67
BtsCI GGATG 2 cut(s) 34, 77
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 284, 290
Csp6I GTAC 3 cut(s) 4, 230, 277
CviAII CATG 1 cut(s) 294
CviJI RGCY 3 cut(s) 67, 105, 122
CviKI_1 RGCY 3 cut(s) 67, 105, 122
CviQI GTAC 3 cut(s) 4, 230, 277
DpnI GATC 1 cut(s) 170
DpnII GATC 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco105I TACGTA 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 284, 290
FaeI CATG 1 cut(s) 297
FaiI YATR 3 cut(s) 270, 282, 295
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 303
FatI CATG 1 cut(s) 293
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 3 cut(s) 120, 123, 135
FokI GGATG 2 cut(s) 21, 64
Fsp4HI GCNGC 3 cut(s) 120, 123, 135
GluI GCNGC 3 cut(s) 120, 123, 135
HaeIII GGCC 1 cut(s) 67
HapII CCGG 3 cut(s) 44, 106, 287
Hin1II CATG 1 cut(s) 297
HinfI GANTC 1 cut(s) 109
HpaII CCGG 3 cut(s) 44, 106, 287
HphI GGTGA 2 cut(s) 33, 249
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 2 cut(s) 75, 86
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 1 cut(s) 270
HpyAV CCTTC 2 cut(s) 48, 188
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 3 cut(s) 125, 206, 225
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 231
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 1 cut(s) 297
Kzo9I GATC 1 cut(s) 168
LguI GCTCTTC 1 cut(s) 109
LpnPI CCDG 6 cut(s) 57, 119, 129, 202, 203, 300
Lsp1109I GCAGC 3 cut(s) 109, 121, 131
MaeII ACGT 1 cut(s) 266
MalI GATC 1 cut(s) 170
MboI GATC 1 cut(s) 168
MboII GAAGA 1 cut(s) 126
MfeI CAATTG 1 cut(s) 207
MluCI AATT 2 cut(s) 207, 301
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 4 cut(s) 39, 42, 72, 143
MseI TTAA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 3 cut(s) 44, 106, 287
MspR9I CCNGG 1 cut(s) 288
MunI CAATTG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 131, 231
NciI CCSGG 1 cut(s) 288
NdeII GATC 1 cut(s) 168
NlaIII CATG 1 cut(s) 297
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 43
NspV TTCGAA 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 3 cut(s) 121, 124, 136
Ppu21I YACGTR 1 cut(s) 267
PshAI GACNNNNGTC 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 2 cut(s) 284, 290
PstI CTGCAG 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 3 cut(s) 5, 231, 278
RsaNI GTAC 3 cut(s) 4, 230, 277
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 3 cut(s) 120, 123, 135
Sau3AI GATC 1 cut(s) 168
Sau96I GGNCC 2 cut(s) 284, 290
ScrFI CCNGG 1 cut(s) 288
SetI ASST 7 cut(s) 19, 124, 182, 191, 205, 231, 269
SfcI CTRYAG 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 112
SinI GGWCC 2 cut(s) 284, 290
SnaBI TACGTA 1 cut(s) 267
Sse9I AATT 2 cut(s) 207, 301
StyD4I CCNGG 1 cut(s) 286
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 1 cut(s) 269
TaqI TCGA 2 cut(s) 112, 167
TasI AATT 2 cut(s) 207, 301
TatI WGTACW 2 cut(s) 3, 276
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 1 cut(s) 54
TseI GCWGC 3 cut(s) 119, 122, 134
TspDTI ATGAA 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 2 cut(s) 284, 290
XmiI GTMKAC 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.