Rmu_sc0025482.1_g000003

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0025482.1
OG0017086
Physical Location & Seq
Forward (+)
3106 .. 4082
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0025482.1_g000003.1.cds

Sequence Viewer

Length: 711 bp
atggaagttggggcagattctgtagatagctcccttgttctggtcaagcaaggagctgaagctagggtttttgagtcaacctttgtgggaaggagatctattgtcaaggaacggttttcgaagaagtacaggcatcctactttggatgcaaaacttacactcaggcgcctcaatgcggaggcgaggtgcatgacgaaagcaagacggcttggggtgcatactccggtgctttacgctgtggacactgtgctgcataccctgacatttgaatatgtggaggggccttctgtaaaagatgtgtttcttgagtttgggttaggaggtggtcgtgtggctgcggaacgtatggatgatattgcaatgcagattggtcaggctattggaaagctgcatgatggtggcctgattcatggggacttgaccacatctaacctgctaattagagctgctactaatcaagtggttgttattgatttcggtttgagctctacatcaacccttccagaagataaagctgtggatttgtatgtactggaacgagctttgctctcgatgcattcttcatgtgggaatgtggtaagacaagcctgttcatatttgctagtttgctgtctgaacagaaaatgcctttatttacatttttactcacatcatgattactctattcaagaaaacaaaaagaattgtgattctattcagaaaatgacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

236

Amino Acids

26.22

Weight (kDa)

8.44

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08120
rosa_chinensis RchiOBHm_Chr3g0476231 RchiOBHm_Chr5g0022281 RchiOBHm_Chr5g0022321
rosa_laevigata RLG00000019416
rosa_multiflora Rmu_sc0025482.1_g000003
rosa_rugosa Rorug05G0073100.1 Rorug05G0073200 Rorug05G0073300
rosa_samantha Rh3BG235400 Rh5AG160000 Rh5CG173800
rosa_wichuraiana Rw5G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 165
AciI CCGC 2 cut(s) 176, 338
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 2 cut(s) 128, 531
AfiI CCNNNNNNNGG 2 cut(s) 40, 175
AgsI TTSAA 2 cut(s) 269, 668
AluBI AGCT 8 cut(s) 30, 56, 62, 388, 446, 486, 515, 542
AluI AGCT 8 cut(s) 30, 56, 62, 388, 446, 486, 515, 542
Alw21I GWGCWC 1 cut(s) 488
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 2 cut(s) 281, 400
ApeKI GCWGC 4 cut(s) 250, 335, 388, 446
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 281
AsuII TTCGAA 1 cut(s) 119
BanI GGYRCC 1 cut(s) 165
BanII GRGCYC 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 4 cut(s) 237, 322, 375, 433
BccI CCATC 1 cut(s) 389
BceAI ACGGC 1 cut(s) 221
BfaI CTAG 2 cut(s) 63, 602
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 441
BglII AGATCT 1 cut(s) 95
BisI GCNGC 4 cut(s) 251, 336, 389, 447
BlsI GCNGC 4 cut(s) 252, 337, 390, 448
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 167, 282
BmsI GCATC 3 cut(s) 136, 142, 543
BplI GAGNNNNNCTC 2 cut(s) 531, 563
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 166
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 175
Bse1I ACTGG 1 cut(s) 537
Bse3DI GCAATG 1 cut(s) 366
BseGI GGATG 3 cut(s) 133, 151, 355
BseLI CCNNNNNNNGG 2 cut(s) 40, 175
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 537
BseXI GCAGC 4 cut(s) 237, 322, 375, 433
BshFI GGCC 2 cut(s) 283, 402
BshNI GGYRCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 488
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 40, 175
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 2 cut(s) 283, 402
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 95
BspACI CCGC 2 cut(s) 176, 338
BspANI GGCC 2 cut(s) 283, 402
BspCNI CTCAG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 167, 282
BspMI ACCTGC 1 cut(s) 441
BspT104I TTCGAA 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 1 cut(s) 537
BssMI GATC 1 cut(s) 95
BssNI GRCGYC 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 114, 247
BstACI GRCGYC 1 cut(s) 166
BstBI TTCGAA 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 161
BstF5I GGATG 3 cut(s) 133, 151, 355
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 1 cut(s) 98
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 214, 553
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 4 cut(s) 237, 322, 375, 433
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 2 cut(s) 283, 402
BtsCI GGATG 3 cut(s) 133, 151, 355
BtsIMutI CAGTG 1 cut(s) 243
BveI ACCTGC 1 cut(s) 441
CaiI CAGNNNCTG 1 cut(s) 20
CciI TCATGA 1 cut(s) 652
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 2 cut(s) 127, 530
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 5 cut(s) 190, 392, 410, 564, 653
CviQI GTAC 2 cut(s) 127, 530
DdeI CTNAG 1 cut(s) 161
DinI GGCGCC 1 cut(s) 167
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 488
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 558
EcoT38I GRGCYC 1 cut(s) 488
EgeI GGCGCC 1 cut(s) 167
EheI GGCGCC 1 cut(s) 167
FaeI CATG 5 cut(s) 193, 395, 413, 567, 656
FaqI GGGAC 1 cut(s) 428
FatI CATG 5 cut(s) 189, 391, 409, 563, 652
Fnu4HI GCNGC 4 cut(s) 251, 336, 389, 447
FokI GGATG 3 cut(s) 120, 158, 362
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 4 cut(s) 251, 336, 389, 447
FspBI CTAG 2 cut(s) 63, 602
GlaI GCGC 1 cut(s) 167
GluI GCNGC 4 cut(s) 251, 336, 389, 447
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 283, 402
HapII CCGG 1 cut(s) 224
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 5 cut(s) 193, 395, 413, 567, 656
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 4 cut(s) 17, 74, 406, 689
HpaII CCGG 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 78, 241
Hpy188I TCNGA 2 cut(s) 615, 699
Hpy188III TCNNGA 5 cut(s) 305, 503, 550, 653, 668
Hpy8I GTNNAC 2 cut(s) 78, 241
HpyAV CCTTC 3 cut(s) 84, 294, 509
HpyCH4III ACNGT 2 cut(s) 114, 247
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 8 cut(s) 149, 189, 217, 253, 359, 364, 391, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 553
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 5 cut(s) 193, 395, 413, 567, 656
HspAI GCGC 1 cut(s) 166
KasI GGCGCC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 95
LmnI GCTCC 2 cut(s) 35, 53
Lsp1109I GCAGC 4 cut(s) 237, 322, 375, 433
LweI GCATC 3 cut(s) 136, 142, 543
MaeI CTAG 2 cut(s) 63, 602
MaeII ACGT 1 cut(s) 343
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 3 cut(s) 133, 518, 552
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 2 cut(s) 438, 682
Mly113I GGCGCC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 5 cut(s) 172, 177, 179, 271, 314
Mph1103I ATGCAT 1 cut(s) 558
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 1 cut(s) 224
Mva1269I GAATGC 1 cut(s) 556
MwoI GCNNNNNNNGC 2 cut(s) 214, 553
NarI GGCGCC 1 cut(s) 166
NdeII GATC 1 cut(s) 95
NlaIII CATG 5 cut(s) 193, 395, 413, 567, 656
NlaIV GGNNCC 2 cut(s) 167, 282
NsiI ATGCAT 1 cut(s) 558
NspV TTCGAA 1 cut(s) 119
PagI TCATGA 1 cut(s) 652
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 17, 406, 689
PkrI GCNGC 4 cut(s) 252, 337, 390, 448
PleI GAGTC 1 cut(s) 82
PluTI GGCGCC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 488
PspN4I GGNNCC 2 cut(s) 167, 282
PspPI GGNCC 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 2 cut(s) 128, 531
RsaNI GTAC 2 cut(s) 127, 530
RseI CAYNNNNRTG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 488
SatI GCNGC 4 cut(s) 251, 336, 389, 447
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 281
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 3 cut(s) 136, 142, 543
SfcI CTRYAG 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 2 cut(s) 438, 682
SsiI CCGC 2 cut(s) 176, 338
SspDI GGCGCC 1 cut(s) 165
SspMI CTAG 2 cut(s) 63, 602
SstI GAGCTC 1 cut(s) 488
TaaI ACNGT 2 cut(s) 114, 247
TaiI ACGT 1 cut(s) 346
TaqI TCGA 2 cut(s) 119, 551
TasI AATT 2 cut(s) 438, 682
TatI WGTACW 2 cut(s) 126, 529
TfiI GAWTC 3 cut(s) 17, 406, 689
TscAI CASTG 1 cut(s) 250
TseI GCWGC 4 cut(s) 250, 335, 388, 446
TspDTI ATGAA 3 cut(s) 398, 552, 582
TspRI CASTG 1 cut(s) 250
XspI CTAG 2 cut(s) 63, 602
Zsp2I ATGCAT 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.