Rorug05G0073200

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017086
Physical Location & Seq
Reverse (-)
6269525 .. 6272958
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0073200.1

Sequence Viewer

Length: 2766 bp
ATGTCTTCTCCCTCTGCAAACCTTTACAAAGTCAAAACGATTTGGCTCTTATCTTCTCTGCTTACCTATTTGTTCATCATTCTCTCCAATGGAGTTGCAGCTCAAAATGATATCAGTGTCACAAACGTTGGAGCAATCATTGATCTTAATTCTCGTATCGGCAAAGAGCAGAAAGTTGCCATGGAAATTGCAGCTGAAAGCTTCAACAACAAGTCCAGCACTCATGAACTAATCCTTCATTTTCGAAATTCAAGCGGAGAACCCTTTCTTGCTGCTTCTGCTGCTGAAGAGTTGATAAAGGAAGAAAAAGTACAAGTGATTGTTGGCATGGAGTATTGGCCAGAAGCAGTTCAAGTAGCTGATCATGTTAAAAACCAATCTCAAGTTGTTCCGGTAATTTCATTTGGTGCACCTCTTATAACTCCTCCAATAATCCAACACCGGTGGCCTTTCTTGATAAGAATGACAAGCGATGGATCAGCTCAAATGAAATGCATTGCAGACATAGTTGCTGCATACAATTGGAAAAGGGTTGTTGTGATATATGAAGACGATGGTTATGGTGGTTATGTTGGATTGCTATCTCTTTTGTCTGAGGCTCTTCAAGATGCTAATGCAAACATTGAGAATCATTTGGTTCTTCCGAGACTAAATAATTCTTCGGAGTCTAAGCCTAATTGGGATGAAGTAGAAGAGCTGTTGAAGCTTCCCACCTTTCAGTCTCGGGTGTTTATTGTTCTTCAATCATCTTTACCAACAGTGACTAATCTGTTCAGAGTGGCTAAGAAGATGGGACTTGTAGGGAGAGAGTCAGCTTGGATATTCACAGAATGTATCACTAGTTTGCTTAACCCTCAAGGTAACTACGATATGGAAGGATCTCTGGGAATCAAGACCTACTACCCCACTGAGTCTAGTTCTTATGCCGAATTCCACAAGGAATTCCAAGCTAAGTATGCAGAAGGAAATAACTCCAAGCCAGGAATTTATACTCTTCGAGCGTACGATACTATTAATGTCATTTCACAGGCCATAGAAAGAATGCCTAATAACACCGCTAGTACTAGCCTTCAAGATATGATAACTAGATTGTTGAGCAAGTACAAGGGTTTAAGTGGGGATATGCACTTCAATAAAGGTGAAGCACTGTTGGATTCTCCTAGACTGAGGATTGTAAATGTTGTGGATGGGAAGATGAACAGAGAGTTAAACTTTTGGACACCAAATGATGGGTTCTCAGAAGGCCTTGACCCAAATAGCGTTGATGTTGTTGGTAAAGTAATTTGGCCTGGGAACCTAAGCCGTACCCCAAAAGGATGGGAAATGCCTAGTGTTGAAAAGCCAATGATAATTGGGGTTCCAGGTAAAACTTCCTTCAGCAAATTTGTAAAGGTGGAGTATAAGGACAATCCTGATGAGAATACGTATGATGGTTTCTGCATTCAAATTTTCTATAAGGTTTTAAGTCGTTTGAATTATCCTCTGCCGTACGAGTTTCATGCTCACGATGGAGACTATGATGGCCTGGTTGAAAAAGTCCATAACAAGACCTATGATGCTGTTGTTGGTGATATAACCGTTCTAGCTGACCGAATGGACAAGGTGGAATTCACTCAACCCTATATGGGGTCAGGGTTGTCAATGATAGTACCAGAAGTAACCGATGAGGAGGCAACATGGATGTTTATGAAACCTTTTACATGGCAAATGTGGGTGGTGACCGGTTCCATTTTAATCTACACAATGTTGGTAGTATGGTTCCTAGAGCGGCCAACCAATCCCGAATTTGGTGGTCCATTGAAGGATCAAATTGGAACAGCAATTTGGTTCACCTTCTCCTCTTTATTCTTTGCTCACAGAGAAAAAGTCTATAGTAACTTAACTCGAGTAGTTTTTATTCTATGGCTGTTTGTTGTATTGATCCTAACCTCAAGCTACACTGCTAATCTCTCTTCAATGCTCACCGTTCAACGACTGAAACCGAATGTAACAGACATTGAAATGCTAAAAAGAACCAATGCACGAGTTGGTTTGGATGGGGATTCATTCGTGTACACATACATGGAGGAGGTTCTTGGATTCAAGGAAGTTAACTTGATGAACGTATCGAGTGAATACGACTATACAGGCTACTTCAAAAACAAAACAATATTAGCTGCCTTTCTCGAAATCCCCTATGCTGAAGTATTCTTGAACAAGTATTGTGATGGATATACTGATAACACTCCTCCCAATAGATTTGGAGGCTTAAGCTTTATGTTTCAGAAAGGCTCTCCCATTGCCAGGGATTTCACTATGGCCATATTAGAGCTATTGGAGAATGGAGAGATGAAGAAACTTCAAAATGAGTGGTTGTCGCCGGAGGAGGAGTGCACGAAGAATTCAACTTCCAATCGACCAGAAAGCTTAAGCCTCGACAGCTTTTCTGGCCTCTATGTAATATCCGGTGCTACTTCTACCTTCTGTTTTCTACTATCTCTTGCTATCTGGCTGAGGAGGTTTCAGCTCCAAGAAACAAATGAAGGCAATGCAAGTCCAAGTAATGAAAATATTTGGAACAAAACTGTCAGAATGGTAAAGTTTTTCAAACTTAGAGATCTTGAAATTCCAAATAGATCTCCAACTTTTGCTAGTTATATTGTGGATTGGACTACTCCAAGGTGGGGGGATTACTCGCCTACTTCCAACACTCCAGAGCAGCCTCCGGTGTTCACACCTGGAGAGATCGAATGCACTCCAGTAGAACATACAGAAACAAGGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

921

Amino Acids

104.18

Weight (kDa)

5.17

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 53 - 378 6.5e-14 Periplasmic binding protein
ANF_receptor PF01094 58 - 395 3e-39 Receptor family ligand binding region
SBP_bac_3 PF00497 466 - 786 1.6e-13 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 468 - 551 1.7e-09 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 567 - 817 4.2e-32 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08120
rosa_chinensis RchiOBHm_Chr3g0476231 RchiOBHm_Chr5g0022281 RchiOBHm_Chr5g0022321
rosa_laevigata RLG00000019416
rosa_multiflora Rmu_sc0025482.1_g000003
rosa_rugosa Rorug05G0073100.1 Rorug05G0073200 Rorug05G0073300
rosa_samantha Rh3BG235400 Rh5AG160000 Rh5CG173800
rosa_wichuraiana Rw5G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 419
AccB7I CCANNNNNTGG 1 cut(s) 1229
AccBSI CCGCTC 1 cut(s) 1768
AciI CCGC 3 cut(s) 255, 1056, 1768
AclI AACGTT 1 cut(s) 126
AclWI GGATC 4 cut(s) 484, 886, 1812, 1915
AcoI YGGCCR 3 cut(s) 338, 1769, 2298
AcuI CTGAAG 3 cut(s) 306, 1360, 2202
AfaI GTAC 8 cut(s) 312, 1004, 1063, 1103, 1306, 1490, 1650, 2054
AfiI CCNNNNNNNGG 6 cut(s) 1229, 1625, 1626, 1787, 2283, 2663
AflII CTTAAG 2 cut(s) 2248, 2407
AgeI ACCGGT 2 cut(s) 441, 1721
AhlI ACTAGT 1 cut(s) 839
AjnI CCWGG 6 cut(s) 979, 1288, 1360, 1524, 2282, 2716
AjuI GAANNNNNNNTTGG 2 cut(s) 1807, 1839
Alw21I GWGCWC 2 cut(s) 412, 2375
Alw26I GTCTC 3 cut(s) 640, 726, 1506
Alw44I GTGCAC 2 cut(s) 408, 2371
AlwI GGATC 4 cut(s) 484, 886, 1812, 1915
Ama87I CYCGRG 2 cut(s) 723, 1884
AoxI GGCC 9 cut(s) 338, 446, 1029, 1243, 1286, 1522, 1769, 2298, 2428
ApaLI GTGCAC 2 cut(s) 408, 2371
ApeKI GCWGC 7 cut(s) 98, 191, 272, 281, 512, 2156, 2698
AseI ATTAAT 1 cut(s) 1014
AsiGI ACCGGT 2 cut(s) 441, 1721
Asp700I GAANNNNTTC 2 cut(s) 264, 348
AspS9I GGNCC 1 cut(s) 1793
AsuHPI GGTGA 5 cut(s) 1151, 1580, 1729, 1822, 1954
AsuII TTCGAA 1 cut(s) 244
AvaI CYCGRG 2 cut(s) 723, 1884
AvaII GGWCC 1 cut(s) 1793
BaeGI GKGCMC 2 cut(s) 412, 2375
BalI TGGCCA 2 cut(s) 340, 2300
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
BauI CACGAG 1 cut(s) 2022
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 2 cut(s) 412, 2375
BbvCI CCTCAGC 1 cut(s) 2492
BbvI GCAGC 7 cut(s) 110, 203, 259, 268, 499, 2143, 2710
BceAI ACGGC 2 cut(s) 1287, 1471
BcgI CGANNNNNNTGC 2 cut(s) 1481, 1515
BciT130I CCWGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 640, 726, 1506
BcuI ACTAGT 1 cut(s) 839
BfmI CTRYAG 1 cut(s) 1870
BfrI CTTAAG 2 cut(s) 2248, 2407
BglII AGATCT 2 cut(s) 2596, 2615
BisI GCNGC 8 cut(s) 99, 192, 273, 282, 513, 1769, 2157, 2699
BlsI GCNGC 8 cut(s) 100, 193, 274, 283, 514, 1770, 2158, 2700
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
Bme18I GGWCC 1 cut(s) 1793
BmeT110I CYCGRG 2 cut(s) 723, 1884
BmgT120I GGNCC 1 cut(s) 1793
BmiI GGNNCC 4 cut(s) 1295, 1359, 1726, 1760
BmrFI CCNGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
BmsI GCATC 2 cut(s) 598, 1546
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 3 cut(s) 2676, 2721, 2739
Bpu10I CCTNAGC 2 cut(s) 1298, 2492
Bpu14I TTCGAA 1 cut(s) 244
BpuEI CTTGAG 3 cut(s) 366, 840, 1915
BsaAI YACGTR 1 cut(s) 1425
BsaBI GATNNNNATC 1 cut(s) 580
BsaJI CCNNGG 4 cut(s) 180, 1289, 2283, 2657
BsaWI WCCGGW 5 cut(s) 391, 441, 1721, 2444, 2704
BsaXI ACNNNNNCTCC 2 cut(s) 2354, 2384
Bsc4I CCNNNNNNNGG 6 cut(s) 1229, 1625, 1626, 1787, 2283, 2663
Bse118I RCCGGY 2 cut(s) 441, 1721
Bse1I ACTGG 1 cut(s) 2738
Bse3DI GCAATG 3 cut(s) 495, 2277, 2533
Bse8I GATNNNNATC 1 cut(s) 580
BseBI CCWGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
BseDI CCNNGG 4 cut(s) 180, 1289, 2283, 2657
BseGI GGATG 5 cut(s) 688, 1192, 1322, 1686, 2041
BseJI GATNNNNATC 1 cut(s) 580
BseLI CCNNNNNNNGG 6 cut(s) 1229, 1625, 1626, 1787, 2283, 2663
BseMI GCAATG 3 cut(s) 495, 2277, 2533
BseMII CTCAG 5 cut(s) 585, 900, 1157, 1251, 2483
BseNI ACTGG 1 cut(s) 2738
BseRI GAGGAG 8 cut(s) 414, 1682, 1828, 2081, 2217, 2378, 2381, 2509
BseSI GKGCMC 2 cut(s) 412, 2375
BseXI GCAGC 7 cut(s) 110, 203, 259, 268, 499, 2143, 2710
BshFI GGCC 9 cut(s) 340, 448, 1031, 1245, 1288, 1524, 1771, 2300, 2430
BshTI ACCGGT 2 cut(s) 441, 1721
BsiHKAI GWGCWC 2 cut(s) 412, 2375
BsiHKCI CYCGRG 2 cut(s) 723, 1884
BsiSI CCGG 6 cut(s) 392, 442, 1722, 2360, 2445, 2705
BsiWI CGTACG 2 cut(s) 1002, 1488
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 6 cut(s) 1229, 1625, 1626, 1787, 2283, 2663
BsmAI GTCTC 3 cut(s) 640, 726, 1506
BsmFI GGGAC 1 cut(s) 807
BsmI GAATGC 3 cut(s) 1047, 1440, 2735
BsnI GGCC 9 cut(s) 340, 448, 1031, 1245, 1288, 1524, 1771, 2300, 2430
BsoBI CYCGRG 2 cut(s) 723, 1884
Bsp119I TTCGAA 1 cut(s) 244
Bsp1286I GDGCHC 2 cut(s) 412, 2375
Bsp1407I TGTACA 1 cut(s) 2052
Bsp143I GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 3 cut(s) 255, 1056, 1768
BspANI GGCC 9 cut(s) 340, 448, 1031, 1245, 1288, 1524, 1771, 2300, 2430
BspCNI CTCAG 5 cut(s) 586, 901, 1158, 1250, 2484
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 4 cut(s) 1295, 1359, 1726, 1760
BspPI GGATC 4 cut(s) 484, 886, 1812, 1915
BspQI GCTCTTC 2 cut(s) 606, 687
BspT104I TTCGAA 1 cut(s) 244
BspTI CTTAAG 2 cut(s) 2248, 2407
BsrBI CCGCTC 1 cut(s) 1768
BsrDI GCAATG 3 cut(s) 495, 2277, 2533
BsrFI RCCGGY 2 cut(s) 441, 1721
BsrGI TGTACA 1 cut(s) 2052
BsrI ACTGG 1 cut(s) 2738
BssAI RCCGGY 2 cut(s) 441, 1721
BssECI CCNNGG 4 cut(s) 180, 1289, 2283, 2657
BssMI GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
BssSI CACGAG 1 cut(s) 2022
BssT1I CCWWGG 2 cut(s) 180, 2657
Bst2BI CACGAG 1 cut(s) 2022
Bst2UI CCWGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
Bst4CI ACNGT 5 cut(s) 760, 1149, 1579, 1966, 2566
Bst6I CTCTTC 5 cut(s) 282, 606, 687, 999, 1957
BstAFI CTTAAG 2 cut(s) 2248, 2407
BstAUI TGTACA 1 cut(s) 2052
BstBAI YACGTR 1 cut(s) 1425
BstBI TTCGAA 1 cut(s) 244
BstDSI CCRYGG 1 cut(s) 180
BstEII GGTNACC 1 cut(s) 1717
BstF5I GGATG 5 cut(s) 688, 1192, 1322, 1686, 2041
BstKTI GATC 9 cut(s) 145, 364, 479, 881, 1807, 1923, 2599, 2618, 2727
BstMAI GTCTC 3 cut(s) 640, 726, 1506
BstMBI GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
BstMWI GCNNNNNNNGC 6 cut(s) 278, 281, 703, 956, 2418, 2427
BstNI CCWGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
BstPI GGTNACC 1 cut(s) 1717
BstSCI CCNGG 6 cut(s) 979, 1288, 1360, 1524, 2282, 2716
BstSFI CTRYAG 1 cut(s) 1870
BstSLI GKGCMC 2 cut(s) 412, 2375
BstSNI TACGTA 1 cut(s) 1425
BstV1I GCAGC 7 cut(s) 110, 203, 259, 268, 499, 2143, 2710
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 3 cut(s) 878, 2596, 2615
BstXI CCANNNNNNTGG 1 cut(s) 1317
BstYI RGATCY 3 cut(s) 878, 2596, 2615
BsuRI GGCC 9 cut(s) 340, 448, 1031, 1245, 1288, 1524, 1771, 2300, 2430
BtgI CCRYGG 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 5 cut(s) 688, 1192, 1322, 1686, 2041
BtsI GCAGTG 1 cut(s) 1938
BtsIMutI CAGTG 5 cut(s) 121, 765, 906, 1145, 1938
CciI TCATGA 1 cut(s) 223
Cfr10I RCCGGY 2 cut(s) 441, 1721
Cfr13I GGNCC 1 cut(s) 1793
Csp6I GTAC 8 cut(s) 311, 1003, 1062, 1102, 1305, 1489, 1649, 2053
CspAI ACCGGT 2 cut(s) 441, 1721
CspCI CAANNNNNGTGG 4 cut(s) 425, 460, 2330, 2365
CviAII CATG 8 cut(s) 181, 224, 328, 365, 1499, 1677, 1701, 2062
CviQI GTAC 8 cut(s) 311, 1003, 1062, 1102, 1305, 1489, 1649, 2053
DpnI GATC 9 cut(s) 144, 363, 478, 880, 1806, 1922, 2598, 2617, 2726
DpnII GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
EaeI YGGCCR 3 cut(s) 338, 1769, 2298
Eam1104I CTCTTC 5 cut(s) 282, 606, 687, 999, 1957
EarI CTCTTC 5 cut(s) 282, 606, 687, 999, 1957
Eco105I TACGTA 1 cut(s) 1425
Eco130I CCWWGG 2 cut(s) 180, 2657
Eco147I AGGCCT 1 cut(s) 1245
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 1793
Eco57I CTGAAG 3 cut(s) 306, 1360, 2202
Eco88I CYCGRG 2 cut(s) 723, 1884
Eco91I GGTNACC 1 cut(s) 1717
EcoO65I GGTNACC 1 cut(s) 1717
EcoRI GAATTC 4 cut(s) 929, 941, 1607, 2380
EcoRII CCWGG 6 cut(s) 979, 1288, 1360, 1524, 2282, 2716
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 180, 2657
EcoT22I ATGCAT 1 cut(s) 497
ErhI CCWWGG 2 cut(s) 180, 2657
FaeI CATG 8 cut(s) 184, 227, 331, 368, 1502, 1680, 1704, 2065
FaqI GGGAC 1 cut(s) 807
FatI CATG 8 cut(s) 180, 223, 327, 364, 1498, 1676, 1700, 2061
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 8 cut(s) 99, 192, 273, 282, 513, 1769, 2157, 2699
FokI GGATG 5 cut(s) 695, 1199, 1329, 1693, 2048
Fsp4HI GCNGC 8 cut(s) 99, 192, 273, 282, 513, 1769, 2157, 2699
GluI GCNGC 8 cut(s) 99, 192, 273, 282, 513, 1769, 2157, 2699
GsuI CTGGAG 3 cut(s) 2676, 2721, 2739
HaeIII GGCC 9 cut(s) 340, 448, 1031, 1245, 1288, 1524, 1771, 2300, 2430
HapII CCGG 6 cut(s) 392, 442, 1722, 2360, 2445, 2705
Hin1II CATG 8 cut(s) 184, 227, 331, 368, 1502, 1680, 1704, 2065
HincII GTYRAC 1 cut(s) 2092
HindII GTYRAC 1 cut(s) 2092
HindIII AAGCTT 4 cut(s) 199, 704, 2251, 2404
HinfI GANTC 8 cut(s) 628, 665, 809, 888, 911, 1154, 2042, 2079
HpaI GTTAAC 1 cut(s) 2092
HpaII CCGG 6 cut(s) 392, 442, 1722, 2360, 2445, 2705
HphI GGTGA 5 cut(s) 1151, 1580, 1729, 1822, 1954
Hpy166II GTNNAC 7 cut(s) 410, 1830, 2053, 2055, 2092, 2373, 2712
Hpy188I TCNGA 7 cut(s) 595, 645, 664, 776, 1240, 2265, 2570
Hpy8I GTNNAC 7 cut(s) 410, 1830, 2053, 2055, 2092, 2373, 2712
HpyCH4III ACNGT 5 cut(s) 760, 1149, 1579, 1966, 2566
HpyCH4IV ACGT 3 cut(s) 126, 1424, 2103
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 281, 703, 956, 2418, 2427
HpySE526I ACGT 3 cut(s) 126, 1424, 2103
Hsp92II CATG 8 cut(s) 184, 227, 331, 368, 1502, 1680, 1704, 2065
Ksp22I TGATCA 1 cut(s) 361
KspAI GTTAAC 1 cut(s) 2092
Kzo9I GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
LguI GCTCTTC 2 cut(s) 606, 687
LmnI GCTCC 2 cut(s) 131, 2511
Lsp1109I GCAGC 7 cut(s) 110, 203, 259, 268, 499, 2143, 2710
LweI GCATC 2 cut(s) 598, 1546
MaeII ACGT 3 cut(s) 126, 1424, 2103
MaeIII GTNAC 7 cut(s) 118, 760, 860, 1657, 1717, 1874, 1987
MalI GATC 9 cut(s) 144, 363, 478, 880, 1806, 1922, 2598, 2617, 2726
MbiI CCGCTC 1 cut(s) 1768
MboI GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
MfeI CAATTG 1 cut(s) 520
MflI RGATCY 3 cut(s) 878, 2596, 2615
MhlI GDGCHC 2 cut(s) 412, 2375
MlsI TGGCCA 2 cut(s) 340, 2300
MluNI TGGCCA 2 cut(s) 340, 2300
MlyI GAGTC 3 cut(s) 674, 818, 920
MmeI TCCRAC 6 cut(s) 109, 460, 553, 1131, 2645, 2709
Mox20I TGGCCA 2 cut(s) 340, 2300
Mph1103I ATGCAT 1 cut(s) 497
MroXI GAANNNNTTC 2 cut(s) 264, 348
MscI TGGCCA 2 cut(s) 340, 2300
MslI CAYNNNNRTG 2 cut(s) 2000, 2060
Msp20I TGGCCA 2 cut(s) 340, 2300
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 2 cut(s) 2248, 2407
MspI CCGG 6 cut(s) 392, 442, 1722, 2360, 2445, 2705
MspR9I CCNGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
MunI CAATTG 1 cut(s) 520
Mva1269I GAATGC 3 cut(s) 1047, 1440, 2735
MvaI CCWGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
MwoI GCNNNNNNNGC 6 cut(s) 278, 281, 703, 956, 2418, 2427
NcoI CCATGG 1 cut(s) 180
NdeII GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
NlaIII CATG 8 cut(s) 184, 227, 331, 368, 1502, 1680, 1704, 2065
NlaIV GGNNCC 4 cut(s) 1295, 1359, 1726, 1760
NmuCI GTSAC 3 cut(s) 118, 760, 1717
NsiI ATGCAT 1 cut(s) 497
NspV TTCGAA 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 1884
PagI TCATGA 1 cut(s) 223
PceI AGGCCT 1 cut(s) 1245
PciSI GCTCTTC 2 cut(s) 606, 687
PctI GAATGC 3 cut(s) 1047, 1440, 2735
PdmI GAANNNNTTC 2 cut(s) 264, 348
PfeI GAWTC 5 cut(s) 628, 888, 1154, 2042, 2079
Pfl23II CGTACG 2 cut(s) 1002, 1488
PflMI CCANNNNNTGG 1 cut(s) 1229
PinAI ACCGGT 2 cut(s) 441, 1721
PkrI GCNGC 8 cut(s) 100, 193, 274, 283, 514, 1770, 2158, 2700
PleI GAGTC 3 cut(s) 673, 817, 919
PpsI GAGTC 3 cut(s) 673, 817, 919
Ppu21I YACGTR 1 cut(s) 1425
PshBI ATTAAT 1 cut(s) 1014
PsiI TTATAA 1 cut(s) 419
Psp1406I AACGTT 1 cut(s) 126
Psp6I CCWGG 6 cut(s) 979, 1288, 1360, 1524, 2282, 2716
PspEI GGTNACC 1 cut(s) 1717
PspGI CCWGG 6 cut(s) 979, 1288, 1360, 1524, 2282, 2716
PspLI CGTACG 2 cut(s) 1002, 1488
PspN4I GGNNCC 4 cut(s) 1295, 1359, 1726, 1760
PspPI GGNCC 1 cut(s) 1793
PspXI VCTCGAGB 1 cut(s) 1884
PsuI RGATCY 3 cut(s) 878, 2596, 2615
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 8 cut(s) 312, 1004, 1063, 1103, 1306, 1490, 1650, 2054
RsaNI GTAC 8 cut(s) 311, 1003, 1062, 1102, 1305, 1489, 1649, 2053
RseI CAYNNNNRTG 2 cut(s) 2000, 2060
SapI GCTCTTC 2 cut(s) 606, 687
SatI GCNGC 8 cut(s) 99, 192, 273, 282, 513, 1769, 2157, 2699
Sau3AI GATC 9 cut(s) 142, 361, 476, 878, 1804, 1920, 2596, 2615, 2724
Sau96I GGNCC 1 cut(s) 1793
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 3 cut(s) 674, 818, 920
ScrFI CCNGG 6 cut(s) 981, 1290, 1362, 1526, 2284, 2718
SduI GDGCHC 2 cut(s) 412, 2375
SfaNI GCATC 2 cut(s) 598, 1546
SfcI CTRYAG 1 cut(s) 1870
Sfr274I CTCGAG 1 cut(s) 1884
SfuI TTCGAA 1 cut(s) 244
SgrAI CRCCGGYG 1 cut(s) 441
SinI GGWCC 1 cut(s) 1793
SlaI CTCGAG 1 cut(s) 1884
SmiMI CAYNNNNRTG 2 cut(s) 2000, 2060
SmlI CTYRAG 6 cut(s) 381, 855, 1884, 1930, 2248, 2407
SmoI CTYRAG 6 cut(s) 381, 855, 1884, 1930, 2248, 2407
SnaBI TACGTA 1 cut(s) 1425
SpeI ACTAGT 1 cut(s) 839
SseBI AGGCCT 1 cut(s) 1245
SsiI CCGC 3 cut(s) 255, 1056, 1768
SspI AATATT 2 cut(s) 2151, 2551
StuI AGGCCT 1 cut(s) 1245
StyD4I CCNGG 6 cut(s) 979, 1288, 1360, 1524, 2282, 2716
StyI CCWWGG 2 cut(s) 180, 2657
TaaI ACNGT 5 cut(s) 760, 1149, 1579, 1966, 2566
TaiI ACGT 3 cut(s) 129, 1427, 2106
TaqI TCGA 8 cut(s) 244, 997, 1885, 2108, 2166, 2395, 2415, 2727
TaqII GACCGA 1 cut(s) 1605
TatI WGTACW 4 cut(s) 310, 1061, 1101, 2052
TauI GCSGC 1 cut(s) 1771
TfiI GAWTC 5 cut(s) 628, 888, 1154, 2042, 2079
TscAI CASTG 5 cut(s) 121, 765, 913, 1152, 1945
TseFI GTSAC 3 cut(s) 118, 760, 1717
TseI GCWGC 7 cut(s) 98, 191, 272, 281, 512, 2156, 2698
Tsp45I GTSAC 3 cut(s) 118, 760, 1717
TspRI CASTG 5 cut(s) 121, 765, 913, 1152, 1945
Van91I CCANNNNNTGG 1 cut(s) 1229
Vha464I CTTAAG 2 cut(s) 2248, 2407
VneI GTGCAC 2 cut(s) 408, 2371
VpaK11BI GGWCC 1 cut(s) 1793
VspI ATTAAT 1 cut(s) 1014
XhoI CTCGAG 1 cut(s) 1884
XmnI GAANNNNTTC 2 cut(s) 264, 348
ZrmI AGTACT 1 cut(s) 1063
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.