Rw5G014300

RIO1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017086
Physical Location & Seq
Forward (+)
17007094 .. 17012177
5084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014300.1

Sequence Viewer

Length: 555 bp
ATGGATGTTGGGGCAGATTCTGTAGATAGCTCCCTTGTTCTGGTGAAGCAAGGAGCTGAAGCTAGGGTTTTTGAGTCAACCTTTGTGGGAAGGAGGTCTATTGTCAAGGAACAGTTTTCGAAGAAGTATAGGCATCCTACTTTGGATGCAAAACTTACACTCAGGCGCCTCAATGCGCAAGACGGCTTGGGGTGCATATATACTCTGGTGCTTTACAATGTGGACACTGTGTTGCATACCCTGACATTTGAATATGTGGAAGGGCCTTCTGTTAAAGATGTGTTTCTTGAGTTTGGGTTAGGAGGTGCTTGTAGTATAAGGATGTTGAACATTGTTATTCTGGTTGCTTTGGCAACTTTTGGTGTATATTTTGTTGAGCATGTTGCCGTTGAAAGATGTTGGGATCTCAGTTCTCAATACAAGTGTATTTGTATGAGGAACAAAAACCATATAAGAGATAAGAAAAAGAGATGGAGAAGGTACCTTTCCAAAATGGAGGTGCACCTTCTCCGACGTCGTCGTCCAAAGCTGACCATGGTTCGTCAATGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

184

Amino Acids

21.29

Weight (kDa)

9.72

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08120
rosa_chinensis RchiOBHm_Chr3g0476231 RchiOBHm_Chr5g0022281 RchiOBHm_Chr5g0022321
rosa_laevigata RLG00000019416
rosa_multiflora Rmu_sc0025482.1_g000003
rosa_rugosa Rorug05G0073100.1 Rorug05G0073200 Rorug05G0073300
rosa_samantha Rh3BG235400 Rh5AG160000 Rh5CG173800
rosa_wichuraiana Rw5G014300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 519
AatII GACGTC 1 cut(s) 517
Acc16I TGCGCA 1 cut(s) 177
Acc65I GGTACC 1 cut(s) 480
AccB1I GGYRCC 2 cut(s) 165, 480
AclWI GGATC 1 cut(s) 411
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 166, 514
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 3 cut(s) 251, 328, 392
AluBI AGCT 4 cut(s) 30, 56, 62, 529
AluI AGCT 4 cut(s) 30, 56, 62, 529
Alw21I GWGCWC 1 cut(s) 504
Alw44I GTGCAC 1 cut(s) 500
AlwI GGATC 1 cut(s) 411
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 1 cut(s) 263
ApaLI GTGCAC 1 cut(s) 500
Asp718I GGTACC 1 cut(s) 480
AspLEI GCGC 2 cut(s) 168, 178
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 2 cut(s) 165, 480
Bbv12I GWGCWC 1 cut(s) 504
BccI CCATC 1 cut(s) 465
BceAI ACGGC 2 cut(s) 199, 371
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 167, 482
BmsI GCATC 2 cut(s) 136, 142
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 308
BsaHI GRCGYC 2 cut(s) 166, 514
BsaJI CCNNGG 1 cut(s) 534
Bsc4I CCNNNNNNNGG 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 4 cut(s) 10, 133, 151, 327
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMII CTCAG 2 cut(s) 175, 421
BseSI GKGCMC 1 cut(s) 504
BshFI GGCC 1 cut(s) 265
BshNI GGYRCC 2 cut(s) 165, 480
BsiHKAI GWGCWC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 40
BsnI GGCC 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 1 cut(s) 403
Bsp19I CCATGG 1 cut(s) 534
BspANI GGCC 1 cut(s) 265
BspCNI CTCAG 2 cut(s) 174, 420
BspLI GGNNCC 2 cut(s) 167, 482
BspPI GGATC 1 cut(s) 411
BspT104I TTCGAA 1 cut(s) 119
BspT107I GGYRCC 2 cut(s) 165, 480
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 2 cut(s) 166, 514
BssT1I CCWWGG 1 cut(s) 534
Bst4CI ACNGT 2 cut(s) 114, 229
BstACI GRCGYC 2 cut(s) 166, 514
BstBI TTCGAA 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 161, 407
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 4 cut(s) 10, 133, 151, 327
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 2 cut(s) 168, 178
BstKTI GATC 1 cut(s) 406
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 1 cut(s) 265
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 4 cut(s) 10, 133, 151, 327
BtsIMutI CAGTG 1 cut(s) 225
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 2 cut(s) 168, 178
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 481
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 2 cut(s) 380, 535
CviJI RGCY 6 cut(s) 30, 56, 62, 186, 265, 529
CviKI_1 RGCY 6 cut(s) 30, 56, 62, 186, 265, 529
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 2 cut(s) 161, 407
DinI GGCGCC 1 cut(s) 167
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
DrdI GACNNNNNNGTC 1 cut(s) 519
DseDI GACNNNNNNGTC 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 534
EgeI GGCGCC 1 cut(s) 167
EheI GGCGCC 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 2 cut(s) 383, 538
FatI CATG 2 cut(s) 379, 534
FokI GGATG 4 cut(s) 17, 120, 158, 334
FspBI CTAG 1 cut(s) 63
FspI TGCGCA 1 cut(s) 177
GlaI GCGC 2 cut(s) 167, 177
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 1 cut(s) 265
HhaI GCGC 2 cut(s) 168, 178
Hin1I GRCGYC 2 cut(s) 166, 514
Hin1II CATG 2 cut(s) 383, 538
Hin6I GCGC 2 cut(s) 166, 176
HinP1I GCGC 2 cut(s) 166, 176
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 2 cut(s) 17, 74
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 3 cut(s) 78, 223, 502
Hpy188I TCNGA 1 cut(s) 512
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 3 cut(s) 78, 223, 502
Hpy99I CGWCG 3 cut(s) 516, 519, 522
HpyAV CCTTC 5 cut(s) 84, 254, 276, 471, 515
HpyCH4III ACNGT 2 cut(s) 114, 229
HpyCH4IV ACGT 1 cut(s) 514
HpyCH4V TGCA 4 cut(s) 149, 195, 235, 502
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 2 cut(s) 161, 407
HpySE526I ACGT 1 cut(s) 514
Hsp92I GRCGYC 2 cut(s) 166, 514
Hsp92II CATG 2 cut(s) 383, 538
HspAI GCGC 2 cut(s) 166, 176
KasI GGCGCC 1 cut(s) 165
KpnI GGTACC 1 cut(s) 484
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 35, 53
LpnPI CCDG 5 cut(s) 26, 148, 191, 254, 326
LweI GCATC 2 cut(s) 136, 142
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 514
MaeIII GTNAC 1 cut(s) 548
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 504
Mly113I GGCGCC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 5 cut(s) 87, 179, 296, 429, 490
MseI TTAA 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 192
NarI GGCGCC 1 cut(s) 166
NcoI CCATGG 1 cut(s) 534
NdeII GATC 1 cut(s) 403
NlaIII CATG 2 cut(s) 383, 538
NlaIV GGNNCC 2 cut(s) 167, 482
NsbI TGCGCA 1 cut(s) 177
NspI RCATGY 1 cut(s) 383
NspV TTCGAA 1 cut(s) 119
PfeI GAWTC 1 cut(s) 17
PflFI GACNNNGTC 1 cut(s) 516
PleI GAGTC 1 cut(s) 82
PluTI GGCGCC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 82
PspN4I GGNNCC 2 cut(s) 167, 482
PspPI GGNCC 1 cut(s) 263
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 403
PsyI GACNNNGTC 1 cut(s) 516
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SaqAI TTAA 1 cut(s) 273
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 1 cut(s) 504
SfaNI GCATC 2 cut(s) 136, 142
SfcI CTRYAG 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SspDI GGCGCC 1 cut(s) 165
SspMI CTAG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 114, 229
TaiI ACGT 1 cut(s) 517
TaqI TCGA 1 cut(s) 119
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 232
TspRI CASTG 1 cut(s) 232
Tth111I GACNNNGTC 1 cut(s) 516
VneI GTGCAC 1 cut(s) 500
XceI RCATGY 1 cut(s) 383
XspI CTAG 1 cut(s) 63
ZraI GACGTC 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.