Rmu_ssc0000004.1_g000001

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000004.1
OG0009609
Physical Location & Seq
Forward (+)
1 .. 1330
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 566 bp
acttcctctcgcccttcgccggcggcagcgtcacctccgacttctccggccacaagctccgcccttcctctctcaatccggtgtcgtctcgtggctctaagcccaaacgcggtgtcgtcaccatggttattccatttcaaaggggaagtgcatgggagcaaccacctccagacctggcatcatatctatacaagaatcgaattgtttacttgggaatgtcgcttgttccttcagtaaccgagttgattcttgcagaatttctctaccttcagtatgaggacgatacaaagcctatctacttgtacataaattccactggaacaaccaaggaaaaggagaggacggagctgctagcggcctccggtggtggtgaaacaaggtttggcctacatccggagaagaagacaggttgggttatgaaggcgccagatctccccaccattggtctgatcagatccagggaggagtggcaggctgattccaatcagtgtaatgttcgaaatgaaaatgatatggatgttgtaaatgacgatgcgatcgatgaaagtcctggcgagcctgagtag

Protein Analysis

187

Amino Acids

20.75

Weight (kDa)

5.07

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 57 - 106 5.7e-08 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 423
AccII CGCG 1 cut(s) 110
AccIII TCCGGA 1 cut(s) 393
AciI CCGC 4 cut(s) 23, 60, 110, 355
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 256, 309
AcuI CTGAAG 2 cut(s) 215, 253
AcyI GRCGYC 1 cut(s) 424
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 4 cut(s) 19, 109, 393, 442
AgsI TTSAA 1 cut(s) 139
AjnI CCWGG 3 cut(s) 173, 457, 549
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 2 cut(s) 57, 348
AluI AGCT 2 cut(s) 57, 348
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 449
Aor13HI TCCGGA 1 cut(s) 393
AoxI GGCC 3 cut(s) 48, 356, 384
ApeKI GCWGC 2 cut(s) 26, 348
ApoI RAATTY 2 cut(s) 256, 309
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AspLEI GCGC 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 24, 111, 382
AsuII TTCGAA 1 cut(s) 498
AsuNHI GCTAGC 1 cut(s) 351
BanI GGYRCC 1 cut(s) 423
BauI CACGAG 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 38, 335
BciT130I CCWGG 3 cut(s) 175, 459, 551
BclI TGATCA 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 352
BfoI RGCGCY 1 cut(s) 427
BglII AGATCT 1 cut(s) 429
BisI GCNGC 4 cut(s) 24, 27, 349, 356
BlsI GCNGC 4 cut(s) 25, 28, 350, 357
Bme1390I CCNGG 3 cut(s) 175, 459, 551
BmiI GGNNCC 1 cut(s) 425
BmrFI CCNGG 3 cut(s) 175, 459, 551
BmsI GCATC 2 cut(s) 187, 522
BmtI GCTAGC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 409
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 482
BsaHI GRCGYC 1 cut(s) 424
BsaJI CCNNGG 3 cut(s) 122, 326, 458
BsaWI WCCGGW 3 cut(s) 78, 361, 393
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 109, 393, 442
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 482
BseAI TCCGGA 1 cut(s) 393
BseBI CCWGG 3 cut(s) 175, 459, 551
BseCI ATCGAT 1 cut(s) 539
BseDI CCNNGG 3 cut(s) 122, 326, 458
BseGI GGATG 2 cut(s) 390, 522
BseJI GATNNNNATC 1 cut(s) 482
BseLI CCNNNNNNNGG 4 cut(s) 19, 109, 393, 442
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 1 cut(s) 478
BseXI GCAGC 2 cut(s) 38, 335
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 1 cut(s) 539
BshFI GGCC 3 cut(s) 50, 358, 386
BshNI GGYRCC 1 cut(s) 423
BshVI ATCGAT 1 cut(s) 539
BsiEI CGRYCG 1 cut(s) 539
BsiSI CCGG 5 cut(s) 20, 47, 79, 362, 394
BslI CCNNNNNNNGG 4 cut(s) 19, 109, 393, 442
BsmAI GTCTC 1 cut(s) 92
BsmBI CGTCTC 1 cut(s) 92
BsnI GGCC 3 cut(s) 50, 358, 386
Bsp119I TTCGAA 1 cut(s) 498
Bsp13I TCCGGA 1 cut(s) 393
Bsp1407I TGTACA 1 cut(s) 302
Bsp143I GATC 4 cut(s) 429, 449, 454, 536
Bsp19I CCATGG 1 cut(s) 122
BspACI CCGC 4 cut(s) 23, 60, 110, 355
BspANI GGCC 3 cut(s) 50, 358, 386
BspCNI CTCAG 1 cut(s) 552
BspDI ATCGAT 1 cut(s) 539
BspEI TCCGGA 1 cut(s) 393
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 1 cut(s) 425
BspOI GCTAGC 1 cut(s) 355
BspPI GGATC 1 cut(s) 449
BspT104I TTCGAA 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 19
BsrGI TGTACA 1 cut(s) 302
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 3 cut(s) 122, 326, 458
BssMI GATC 4 cut(s) 429, 449, 454, 536
BssNI GRCGYC 1 cut(s) 424
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 2 cut(s) 122, 326
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 3 cut(s) 175, 459, 551
BstACI GRCGYC 1 cut(s) 424
BstAUI TGTACA 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 4 cut(s) 21, 353, 473, 556
BstDEI CTNAG 2 cut(s) 98, 560
BstDSI CCRYGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 390, 522
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 4 cut(s) 432, 452, 457, 539
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 4 cut(s) 429, 449, 454, 536
BstMCI CGRYCG 1 cut(s) 539
BstNI CCWGG 3 cut(s) 175, 459, 551
BstSCI CCNGG 3 cut(s) 173, 457, 549
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 38, 335
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 2 cut(s) 429, 454
BstYI RGATCY 2 cut(s) 429, 454
Bsu15I ATCGAT 1 cut(s) 539
BsuRI GGCC 3 cut(s) 50, 358, 386
BsuTUI ATCGAT 1 cut(s) 539
BtgI CCRYGG 1 cut(s) 122
BtsCI GGATG 2 cut(s) 390, 522
BtsIMutI CAGTG 2 cut(s) 314, 493
Cac8I GCNNGC 4 cut(s) 21, 353, 473, 556
CfoI GCGC 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 19
ClaI ATCGAT 1 cut(s) 539
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 303
CviAII CATG 2 cut(s) 123, 152
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 2 cut(s) 98, 560
DinI GGCGCC 1 cut(s) 425
DpnI GATC 4 cut(s) 431, 451, 456, 538
DpnII GATC 4 cut(s) 429, 449, 454, 536
EaeI YGGCCR 1 cut(s) 48
EciI GGCGGA 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 122, 326
Eco57I CTGAAG 2 cut(s) 215, 253
EcoRII CCWGG 3 cut(s) 173, 457, 549
EcoT14I CCWWGG 2 cut(s) 122, 326
EgeI GGCGCC 1 cut(s) 425
EheI GGCGCC 1 cut(s) 425
ErhI CCWWGG 2 cut(s) 122, 326
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 2 cut(s) 126, 155
FaiI YATR 8 cut(s) 124, 153, 183, 189, 275, 307, 418, 514
FatI CATG 2 cut(s) 122, 151
FbaI TGATCA 1 cut(s) 449
Fnu4HI GCNGC 4 cut(s) 24, 27, 349, 356
FokI GGATG 2 cut(s) 377, 529
Fsp4HI GCNGC 4 cut(s) 24, 27, 349, 356
FspBI CTAG 1 cut(s) 352
GlaI GCGC 1 cut(s) 425
GluI GCNGC 4 cut(s) 24, 27, 349, 356
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 3 cut(s) 50, 358, 386
HapII CCGG 5 cut(s) 20, 47, 79, 362, 394
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 426
Hin1I GRCGYC 1 cut(s) 424
Hin1II CATG 2 cut(s) 126, 155
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HinfI GANTC 3 cut(s) 195, 246, 478
HpaII CCGG 5 cut(s) 20, 47, 79, 362, 394
HphI GGTGA 3 cut(s) 24, 111, 382
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 3 cut(s) 39, 449, 454
Hpy188III TCNNGA 2 cut(s) 169, 394
Hpy8I GTNNAC 1 cut(s) 207
HpyAV CCTTC 5 cut(s) 24, 74, 239, 277, 414
HpyCH4V TGCA 2 cut(s) 151, 253
HpyF3I CTNAG 2 cut(s) 98, 560
Hsp92I GRCGYC 1 cut(s) 424
Hsp92II CATG 2 cut(s) 126, 155
HspAI GCGC 1 cut(s) 424
KasI GGCGCC 1 cut(s) 423
Kpn2I TCCGGA 1 cut(s) 393
KroI GCCGGC 1 cut(s) 19
KroNI GCCGGC 1 cut(s) 21
Ksp22I TGATCA 1 cut(s) 449
Kzo9I GATC 4 cut(s) 429, 449, 454, 536
LmnI GCTCC 3 cut(s) 62, 156, 345
Lsp1109I GCAGC 2 cut(s) 38, 335
LweI GCATC 2 cut(s) 187, 522
MaeI CTAG 1 cut(s) 352
MaeIII GTNAC 3 cut(s) 30, 117, 234
MalI GATC 4 cut(s) 431, 451, 456, 538
MboI GATC 4 cut(s) 429, 449, 454, 536
MboII GAAGA 2 cut(s) 411, 414
MflI RGATCY 2 cut(s) 429, 454
MluCI AATT 3 cut(s) 200, 256, 309
Mly113I GGCGCC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 62
MnlI CCTC 8 cut(s) 16, 45, 78, 176, 270, 332, 369, 456
MreI CGCCGGCG 1 cut(s) 19
MroI TCCGGA 1 cut(s) 393
MroNI GCCGGC 1 cut(s) 19
MspI CCGG 5 cut(s) 20, 47, 79, 362, 394
MspR9I CCNGG 3 cut(s) 175, 459, 551
MvaI CCWGG 3 cut(s) 175, 459, 551
MvnI CGCG 1 cut(s) 110
NaeI GCCGGC 1 cut(s) 21
NarI GGCGCC 1 cut(s) 424
NcoI CCATGG 1 cut(s) 122
NdeII GATC 4 cut(s) 429, 449, 454, 536
NgoMIV GCCGGC 1 cut(s) 19
NheI GCTAGC 1 cut(s) 351
NlaIII CATG 2 cut(s) 126, 155
NlaIV GGNNCC 1 cut(s) 425
NmuCI GTSAC 2 cut(s) 30, 117
NspV TTCGAA 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PdiI GCCGGC 1 cut(s) 21
PfeI GAWTC 3 cut(s) 195, 246, 478
PkrI GCNGC 4 cut(s) 25, 28, 350, 357
Ple19I CGATCG 1 cut(s) 539
PluTI GGCGCC 1 cut(s) 427
Psp6I CCWGG 3 cut(s) 173, 457, 549
PspGI CCWGG 3 cut(s) 173, 457, 549
PspN4I GGNNCC 1 cut(s) 425
PsuI RGATCY 2 cut(s) 429, 454
PvuI CGATCG 1 cut(s) 539
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
SatI GCNGC 4 cut(s) 24, 27, 349, 356
Sau3AI GATC 4 cut(s) 429, 449, 454, 536
ScrFI CCNGG 3 cut(s) 175, 459, 551
SetI ASST 8 cut(s) 37, 59, 168, 176, 269, 350, 382, 411
SfaNI GCATC 2 cut(s) 187, 522
SfoI GGCGCC 1 cut(s) 425
SfuI TTCGAA 1 cut(s) 498
SgrAI CRCCGGYG 1 cut(s) 19
Sse9I AATT 3 cut(s) 200, 256, 309
SsiI CCGC 4 cut(s) 23, 60, 110, 355
SspDI GGCGCC 1 cut(s) 423
SspMI CTAG 1 cut(s) 352
StyD4I CCNGG 3 cut(s) 173, 457, 549
StyI CCWWGG 2 cut(s) 122, 326
TaqI TCGA 3 cut(s) 198, 498, 539
TasI AATT 3 cut(s) 200, 256, 309
TatI WGTACW 1 cut(s) 302
TauI GCSGC 2 cut(s) 26, 358
TfiI GAWTC 3 cut(s) 195, 246, 478
TscAI CASTG 2 cut(s) 321, 493
TseFI GTSAC 2 cut(s) 30, 117
TseI GCWGC 2 cut(s) 26, 348
Tsp45I GTSAC 2 cut(s) 30, 117
TspDTI ATGAA 3 cut(s) 433, 518, 557
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 2 cut(s) 321, 493
XapI RAATTY 2 cut(s) 256, 309
XspI CTAG 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.