Rmu_ssc0000011.1_g000077

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000011.1
OG0017412
Physical Location & Seq
Reverse (-)
317740 .. 318562
823 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
atgaaaacagaaaggtccagagtgtacctcggctcacttccagataataataagtggtacttaccattgtctcaccacctttgtattctaaaaagagttgtcaagaatggctcgggtgcaaatccgttgtcaagaagttacaaaaggagtttcaatggatttgctgtgaagctcactgaccttgaaagacaaaagattgctaacaggaacgaggtggtctctgtctttccaagcacaactttccgacttcaaacaacaagattgtgggacttcaacggtcaaaatgagaaaatccatcgaaatgccacagtagtgattggtgtccttgacacaggaatctggcccgaatcgggtagcttcaaaaatgaaggtttcagtccggctccaagcagtggaaaggtgcttgtgcaggtggaaaaaacttcacttgcaacaacaagatcagcagtgtacctcaacagcagcagggaacgccctaaaggactagtacaaggtgctacaagaggtggagttccctttgcgagaattgctgcatataaagtctgcaatggcgaatggtgccgttcagaggatccgatagcaataggtgcttttcatgcaactgagaaagggatactaacaactaaaactgcaggcaacgatggttatagagctggttctgtaacaaagtgtggcaccatggttgctaactacagttgcagcaagagatcgtcggataattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

240

Amino Acids

26.35

Weight (kDa)

9.95

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 25 - 83 3.5e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 2 cut(s) 558, 674
AccB7I CCANNNNNTGG 1 cut(s) 392
AclWI GGATC 2 cut(s) 566, 579
AfaI GTAC 4 cut(s) 26, 59, 452, 489
AfiI CCNNNNNNNGG 3 cut(s) 350, 392, 568
AgsI TTSAA 5 cut(s) 154, 185, 251, 274, 361
AhlI ACTAGT 1 cut(s) 484
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 3 cut(s) 172, 357, 653
AluI AGCT 3 cut(s) 172, 357, 653
Alw26I GTCTC 2 cut(s) 75, 223
AlwI GGATC 2 cut(s) 566, 579
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 3 cut(s) 462, 530, 699
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AspS9I GGNCC 2 cut(s) 15, 342
AsuHPI GGTGA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 15
BamHI GGATCC 1 cut(s) 571
BanI GGYRCC 2 cut(s) 558, 674
BbvI GCAGC 3 cut(s) 474, 517, 711
BccI CCATC 2 cut(s) 303, 635
BceAI ACGGC 1 cut(s) 546
BciVI GTATCC 1 cut(s) 606
BcoDI GTCTC 2 cut(s) 75, 223
BcuI ACTAGT 1 cut(s) 484
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 2 cut(s) 630, 691
BfuAI ACCTGC 1 cut(s) 400
BfuI GTATCC 1 cut(s) 606
BisI GCNGC 3 cut(s) 463, 531, 700
BlsI GCNGC 3 cut(s) 464, 532, 701
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 15, 342
BmiI GGNNCC 4 cut(s) 384, 560, 573, 676
BplI GAGNNNNNCTC 4 cut(s) 12, 44, 203, 235
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 28, 678
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 392, 568
Bse3DI GCAATG 1 cut(s) 553
BseDI CCNNGG 2 cut(s) 28, 678
BseLI CCNNNNNNNGG 3 cut(s) 350, 392, 568
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 1 cut(s) 594
BseXI GCAGC 3 cut(s) 474, 517, 711
BsgI GTGCAG 1 cut(s) 428
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 2 cut(s) 558, 674
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 3 cut(s) 350, 392, 568
BsmAI GTCTC 2 cut(s) 75, 223
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 223
BsoBI CYCGRG 1 cut(s) 112
Bsp143I GATC 3 cut(s) 440, 571, 707
Bsp19I CCATGG 1 cut(s) 678
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 384, 560, 573, 676
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 2 cut(s) 566, 579
BspT107I GGYRCC 2 cut(s) 558, 674
BspTNI GGTCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 553
BssECI CCNNGG 2 cut(s) 28, 678
BssMI GATC 3 cut(s) 440, 571, 707
BssT1I CCWWGG 1 cut(s) 678
Bst4CI ACNGT 3 cut(s) 278, 310, 695
BstAPI GCANNNNNTGC 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 634
BstDEI CTNAG 1 cut(s) 603
BstDSI CCRYGG 1 cut(s) 678
BstKTI GATC 3 cut(s) 443, 574, 710
BstMAI GTCTC 2 cut(s) 75, 223
BstMBI GATC 3 cut(s) 440, 571, 707
BstMWI GCNNNNNNNGC 5 cut(s) 471, 527, 558, 587, 596
BstSFI CTRYAG 2 cut(s) 630, 691
BstV1I GCAGC 3 cut(s) 474, 517, 711
BstX2I RGATCY 1 cut(s) 571
BstYI RGATCY 1 cut(s) 571
BsuI GTATCC 1 cut(s) 606
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 678
BtsI GCAGTG 2 cut(s) 397, 453
BtsIMutI CAGTG 3 cut(s) 174, 397, 453
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 1 cut(s) 634
Cfr13I GGNCC 2 cut(s) 15, 342
Csp6I GTAC 4 cut(s) 25, 58, 451, 488
CviAII CATG 2 cut(s) 596, 679
CviJI RGCY 7 cut(s) 33, 111, 172, 343, 357, 383, 653
CviKI_1 RGCY 7 cut(s) 33, 111, 172, 343, 357, 383, 653
CviQI GTAC 4 cut(s) 25, 58, 451, 488
DdeI CTNAG 1 cut(s) 603
DpnI GATC 3 cut(s) 442, 573, 709
DpnII GATC 3 cut(s) 440, 571, 707
Eco130I CCWWGG 1 cut(s) 678
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 2 cut(s) 599, 682
FaiI YATR 5 cut(s) 535, 537, 597, 648, 680
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 223, 255
FaqI GGGAC 1 cut(s) 281
FatI CATG 2 cut(s) 595, 678
Fnu4HI GCNGC 3 cut(s) 463, 531, 700
Fsp4HI GCNGC 3 cut(s) 463, 531, 700
FspBI CTAG 1 cut(s) 485
GluI GCNGC 3 cut(s) 463, 531, 700
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 380
Hin1II CATG 2 cut(s) 599, 682
HinfI GANTC 2 cut(s) 336, 347
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 2 cut(s) 25, 451
Hpy188I TCNGA 4 cut(s) 245, 568, 576, 715
Hpy188III TCNNGA 4 cut(s) 18, 41, 103, 132
Hpy8I GTNNAC 2 cut(s) 25, 451
Hpy99I CGWCG 1 cut(s) 715
HpyAV CCTTC 1 cut(s) 362
HpyCH4III ACNGT 3 cut(s) 278, 310, 695
HpyCH4V TGCA 8 cut(s) 119, 409, 431, 533, 546, 599, 632, 699
HpyF10VI GCNNNNNNNGC 5 cut(s) 471, 527, 558, 587, 596
HpyF3I CTNAG 1 cut(s) 603
Hsp92II CATG 2 cut(s) 599, 682
Kzo9I GATC 3 cut(s) 440, 571, 707
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 3 cut(s) 474, 517, 711
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 137, 661
MalI GATC 3 cut(s) 442, 573, 709
MboI GATC 3 cut(s) 440, 571, 707
MflI RGATCY 1 cut(s) 571
MluCI AATT 2 cut(s) 525, 718
MmeI TCCRAC 2 cut(s) 268, 693
MnlI CCTC 5 cut(s) 38, 205, 464, 497, 562
MslI CAYNNNNRTG 2 cut(s) 300, 311
MspI CCGG 1 cut(s) 380
MwoI GCNNNNNNNGC 5 cut(s) 471, 527, 558, 587, 596
NcoI CCATGG 1 cut(s) 678
NdeII GATC 3 cut(s) 440, 571, 707
NlaIII CATG 2 cut(s) 599, 682
NlaIV GGNNCC 4 cut(s) 384, 560, 573, 676
NmeAIII GCCGAG 1 cut(s) 9
OliI CACNNNNGTG 1 cut(s) 311
PaqCI CACCTGC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 336, 347
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 3 cut(s) 464, 532, 701
PspN4I GGNNCC 4 cut(s) 384, 560, 573, 676
PspPI GGNCC 2 cut(s) 15, 342
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 571
RsaI GTAC 4 cut(s) 26, 59, 452, 489
RsaNI GTAC 4 cut(s) 25, 58, 451, 488
RseI CAYNNNNRTG 2 cut(s) 300, 311
SatI GCNGC 3 cut(s) 463, 531, 700
Sau3AI GATC 3 cut(s) 440, 571, 707
Sau96I GGNCC 2 cut(s) 15, 342
SfcI CTRYAG 2 cut(s) 630, 691
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 300, 311
SpeI ACTAGT 1 cut(s) 484
Sse9I AATT 2 cut(s) 525, 718
SspMI CTAG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 3 cut(s) 278, 310, 695
TaqI TCGA 1 cut(s) 298
TasI AATT 2 cut(s) 525, 718
TatI WGTACW 1 cut(s) 487
TfiI GAWTC 2 cut(s) 336, 347
TscAI CASTG 3 cut(s) 181, 397, 453
TseI GCWGC 3 cut(s) 462, 530, 699
TspDTI ATGAA 3 cut(s) 17, 381, 584
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 3 cut(s) 181, 397, 453
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 15
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.