Rmu_ssc0000078.1_g000001

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000078.1
OG0012794
Physical Location & Seq
Forward (+)
40 .. 4883
4844 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2721 bp
atgcgtacaccgggtggtgacgaagatcattccctgcgtataccagttggaagcaattgtcctgagagctcaagtcaggagtacccatctctggatgatattatggctcagaggccaaatagtgataatctggttgaagttgtggcggaattgacaaatactgtagatggaggcgtttcaggcaattcatatggaggtatccggacaaaaatgctttccaaatcaagatcagaattttatgttaagtctacattgaagggtaagggtgttattttcaaaggtgatggcggtcatgttgaatctaagaaccttaacagtacaaaggttactggtggttctaaggctacttctgattcaatacagagtttggatgccaaaattgttatgccttcttccaatgatctgaacgctgtgcccaaaccaccagccttggaacatgatgaaatcagcttaagagaatggctgagaattggacgtcctaaagtcaataaagttgagtgtctgtatgtattcaaacaaattgcaaatctcgtggatcattttcactctcaaggagttgctttgcaagaattgcgcccttctttttttaaggtgtcaccttcaaatgaggtcaagtatgttggcttaccaatcccaaaagagattttagaaagttctatggatcaaggtatctgtcattcagaacatagtccaatgagaaaaatgtcaggtgagcaagaatttgcttctgttggttccagtgcaaagaagcaaaagatcagccagaacactagattagttaggcaatggcctcagttccccactagttcccgtttccaacgtgaaactaggaatgctagccacatcaacatcaatggtctgcaaaatagaagtcctcatatgtataatgcagctcaacaacacttgacttcaatcactgagcaattggaagaaaagtggtatattagtcctgaagaactcaatgaagggagctgtacagttttgtcaaatatctacagtctgggcgttcttttttttgagttgctggtccgttttgactcaaatagtgccattgtctcagcaatgttcaatctgcgccatagaattcttcctccgaattttctgtcagaatacgctaaagaggctggattttgtctttggttacttcatcctgaaccgtcttcacgtccaacaacaagggaaattctacaatcagaagtagttaatggattacaggagatgtgtgaagagaaagtggcatcatctgtggtcgaagaggatgctgaattggaactattatcccatttcctcacgtcactgaaagaaaagaagcagaaggatgcctccaagttgatggcaagcattagggttttagaagcagatgctgaagaagctgagaggaggcactattcaagaaatcccgactggtacaaagagtccctcggtggatggaaaaacgcatgtaaagaagcttcaagatcagaggtgctttctccattaccccctcttcctagttcaaatgagtcaaggttaacgaggaatattgatcagcttgagagtgcttacttctctatgagatctatgattcagcctcctgaagctgattgtaatatacgcatagataaagaattgttaaaatctcgcaagaattggaatttggaatcagatgatgacgaaaagaagactcctaccgatcaattgggagctatctttgatggtttatgcaagtatgatcggtacagtaagtttgaagtatgtggagtactaagaaatggagatttcaacagtaactcaaatttgatttgctctttgagttttgatcgggatgaggactacattgcttctgctggggtttcaaagaaaataaagatttatgagttcaatgcacttttcaatgattctgttgatattcattatccatcgatcgagatgtcaaatgaatcaaagattagctgtatttgctggaacaactacataaaaaactatctcacttcaactgattatgaaggtgtagtgaagttgtgggatgtcagcactggtcaagagtttgctcgatacagagggcatgaacaaaaggcttggtccgttgacttctctcaagtagatcctacaaaattagccagcgggagtgatgacggttctgtgaaactctggagcattaatgagaaaaactgtttaggcaccatcagaaacagaaacattgcaaatgtatgctgtgtacagttctctgctcactccactaatttgctggcctttgggtctgcggactacaacacatattgctatgatcttcgtaatgctagaacgccctggtgtgtattggttggtcaccaaaaaagcgtaagctatgtcaaattcttggactctgagacccttgtttctgcatccactgacaacacattaaagctatgggatctcaacaaaaccattgccagtggtccatccaccaatgcttgcagcttaaccctcagtgggcataccaatgagaagaactttgtgggcttatccgtttctaatggttatatagcttgcggttcagaaacaaatgaggtctatgcttattatagatctctgcctatgccgatcacttcatacaaatttggctccattgattctataaccggaaaggagactgatgatattaatggatcatttgtttcaagtgtctgctggagacagaaatcaaacatggtggttgccgccaattcaagcgggtgcataaaagtgttgcagattgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
Pkinase WD40

Protein Analysis

906

Amino Acids

101.28

Weight (kDa)

5.91

Isoelectric Point (pI)

49.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 305 - 399 7e-06 Protein kinase domain
Beta-prop_WDR3_1st PF25173 590 - 756 6.7e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 590 - 847 5.9e-32 WDR5 beta-propeller domain
WDR55 PF24796 592 - 723 6.7e-07 WDR55
Beta-prop_TEP1_2nd PF25047 593 - 712 7.4e-06 TEP-1 second beta-propeller
WD40_Gbeta PF25391 594 - 760 6.2e-09 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 594 - 800 1.8e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 596 - 751 5.2e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 596 - 715 2.2e-06 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 599 - 683 7.1e-08 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 605 - 797 5.2e-14 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 638 - 725 5.4e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 639 - 722 6.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 646 - 799 1.7e-06 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 654 - 801 3.8e-09 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 656 - 745 1.8e-07 EIF3I
Beta-prop_EML_2 PF23414 657 - 802 4e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 677 - 712 6.6e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 685 - 802 5.2e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 697 - 847 9.6e-20 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 699 - 801 1.2e-10 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 725 - 902 4.2e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 728 - 840 2e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 731 - 904 2.8e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11110
fragaria_vesca FvH4_5g04010 FvH4_5g04010
malus_domestica MD06G1101400.v1.1 MD14G1121100.v1.1
prunus_persica Prupe.5G114800_v2.0.a1 Prupe.5G114800_v2.0.a1
pyrus_communis pycom06g09660
rosa_chinensis RchiOBHm_Chr7g0195501
rosa_laevigata RLG00000004114
rosa_multiflora Rmu_ssc0000078.1_g000001
rosa_roxburghii Rroxscaffold_3G00260410
rosa_rugosa Rorug07G0023900
rosa_samantha Rh7AG151400 Rh7BG153100 Rh7CG156600 Rh7DG152400
rosa_wichuraiana Rw7G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 478
AccB1I GGYRCC 1 cut(s) 2159
AccI GTMKAC 2 cut(s) 40, 248
AccIII TCCGGA 1 cut(s) 201
AciI CCGC 7 cut(s) 146, 288, 2103, 2243, 2511, 2679, 2691
AclWI GGATC 5 cut(s) 543, 669, 2078, 2400, 2635
AcsI RAATTY 9 cut(s) 233, 719, 1083, 1096, 1182, 1633, 1774, 2333, 2576
AcuI CTGAAG 3 cut(s) 972, 1386, 1596
AcyI GRCGYC 1 cut(s) 475
AdeI CACNNNGTG 1 cut(s) 14
AfaI GTAC 8 cut(s) 7, 83, 319, 976, 1409, 1718, 1743, 2199
AfiI CCNNNNNNNGG 2 cut(s) 91, 2451
AflII CTTAAG 1 cut(s) 451
AhlI ACTAGT 1 cut(s) 803
AjiI CACGTC 2 cut(s) 1166, 1293
AjnI CCWGG 1 cut(s) 2288
AjuI GAANNNNNNNTTGG 2 cut(s) 1619, 1651
Alw21I GWGCWC 1 cut(s) 71
Alw26I GTCTC 4 cut(s) 1060, 2342, 2603, 2647
AlwI GGATC 5 cut(s) 543, 669, 2078, 2400, 2635
AlwNI CAGNNNCTG 1 cut(s) 1364
Aor13HI TCCGGA 1 cut(s) 201
AoxI GGCC 3 cut(s) 113, 788, 2229
ApeKI GCWGC 2 cut(s) 890, 2436
ApoI RAATTY 9 cut(s) 233, 719, 1083, 1096, 1182, 1633, 1774, 2333, 2576
ArsI GACNNNNNNTTYG 4 cut(s) 856, 888, 1088, 1120
AseI ATTAAT 2 cut(s) 2139, 2622
AspLEI GCGC 2 cut(s) 576, 1077
AspS9I GGNCC 3 cut(s) 1027, 2061, 2417
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 5 cut(s) 29, 293, 588, 722, 2300
AsuNHI GCTAGC 1 cut(s) 836
AvaII GGWCC 3 cut(s) 1027, 2061, 2417
BaeGI GKGCMC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 2159
BanII GRGCYC 1 cut(s) 71
BarI GAAGNNNNNNTAC 2 cut(s) 1435, 1467
BauI CACGAG 1 cut(s) 530
BbsI GAAGAC 2 cut(s) 1152, 1667
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 902, 2448
BccI CCATC 9 cut(s) 94, 161, 278, 1327, 1422, 1688, 1906, 2171, 2428
BcgI CGANNNNNNTGC 2 cut(s) 1241, 1275
BciT130I CCWGG 1 cut(s) 2290
BciVI GTATCC 1 cut(s) 209
BclI TGATCA 1 cut(s) 1525
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 4 cut(s) 1060, 2342, 2603, 2647
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 6 cut(s) 771, 804, 828, 837, 1491, 2280
BfmI CTRYAG 2 cut(s) 162, 994
BfrI CTTAAG 1 cut(s) 451
BfuI GTATCC 1 cut(s) 209
BglII AGATCT 2 cut(s) 1556, 2546
BisI GCNGC 3 cut(s) 891, 2437, 2679
BlsI GCNGC 3 cut(s) 892, 2438, 2680
BmcAI AGTACT 1 cut(s) 1743
Bme1390I CCNGG 2 cut(s) 12, 2290
Bme18I GGWCC 3 cut(s) 1027, 2061, 2417
BmgBI CACGTC 2 cut(s) 1166, 1293
BmgT120I GGNCC 3 cut(s) 1027, 2061, 2417
BmiI GGNNCC 3 cut(s) 736, 2161, 2584
BmrFI CCNGG 2 cut(s) 12, 2290
BmsI GCATC 6 cut(s) 361, 1247, 1249, 1309, 1351, 2372
BmtI GCTAGC 1 cut(s) 840
BpiI GAAGAC 2 cut(s) 1152, 1667
BpmI CTGGAG 2 cut(s) 2152, 2671
BpuEI CTTGAG 4 cut(s) 55, 534, 1553, 2061
BpuMI CCSGG 1 cut(s) 12
Bsa29I ATCGAT 1 cut(s) 1901
BsaHI GRCGYC 1 cut(s) 475
BsaI GGTCTC 1 cut(s) 2342
BsaJI CCNNGG 3 cut(s) 429, 1420, 2288
BsaWI WCCGGW 2 cut(s) 201, 2600
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 2451
Bse1I ACTGG 6 cut(s) 44, 334, 738, 1409, 2020, 2412
Bse3DI GCAATG 5 cut(s) 791, 1068, 1815, 2178, 2406
BseAI TCCGGA 1 cut(s) 201
BseBI CCWGG 1 cut(s) 2290
BseCI ATCGAT 1 cut(s) 1901
BseDI CCNNGG 3 cut(s) 429, 1420, 2288
BseLI CCNNNNNNNGG 2 cut(s) 91, 2451
BseMI GCAATG 5 cut(s) 791, 1068, 1815, 2178, 2406
BseMII CTCAG 9 cut(s) 54, 122, 455, 806, 909, 1071, 1365, 2337, 2461
BseNI ACTGG 6 cut(s) 44, 334, 738, 1409, 2020, 2412
BseRI GAGGAG 1 cut(s) 1393
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 2 cut(s) 902, 2448
BseYI CCCAGC 1 cut(s) 1826
Bsh1285I CGRYCG 1 cut(s) 1905
BshFI GGCC 3 cut(s) 115, 790, 2231
BshNI GGYRCC 1 cut(s) 2159
BshVI ATCGAT 1 cut(s) 1901
BsiEI CGRYCG 1 cut(s) 1905
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 3 cut(s) 11, 202, 2601
BslFI GGGAC 1 cut(s) 1402
BslI CCNNNNNNNGG 2 cut(s) 91, 2451
BsmAI GTCTC 4 cut(s) 1060, 2342, 2603, 2647
BsmFI GGGAC 1 cut(s) 1402
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 3 cut(s) 115, 790, 2231
Bso31I GGTCTC 1 cut(s) 2342
Bsp1286I GDGCHC 2 cut(s) 71, 417
Bsp13I TCCGGA 1 cut(s) 201
Bsp1407I TGTACA 2 cut(s) 974, 2197
BspACI CCGC 7 cut(s) 146, 288, 2103, 2243, 2511, 2679, 2691
BspANI GGCC 3 cut(s) 115, 790, 2231
BspCNI CTCAG 9 cut(s) 55, 121, 456, 805, 910, 1070, 1366, 2338, 2460
BspDI ATCGAT 1 cut(s) 1901
BspEI TCCGGA 1 cut(s) 201
BspLI GGNNCC 3 cut(s) 736, 2161, 2584
BspOI GCTAGC 1 cut(s) 840
BspPI GGATC 5 cut(s) 543, 669, 2078, 2400, 2635
BspT107I GGYRCC 1 cut(s) 2159
BspTI CTTAAG 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 2342
BsrDI GCAATG 5 cut(s) 791, 1068, 1815, 2178, 2406
BsrGI TGTACA 2 cut(s) 974, 2197
BsrI ACTGG 6 cut(s) 44, 334, 738, 1409, 2020, 2412
BssECI CCNNGG 3 cut(s) 429, 1420, 2288
BssNAI GTATAC 1 cut(s) 41
BssNI GRCGYC 1 cut(s) 475
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 429
Bst1107I GTATAC 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 2290
Bst6I CTCTTC 3 cut(s) 1221, 1248, 1491
BstACI GRCGYC 1 cut(s) 475
BstAFI CTTAAG 1 cut(s) 451
BstAPI GCANNNNNTGC 1 cut(s) 571
BstAUI TGTACA 2 cut(s) 974, 2197
BstC8I GCNNGC 6 cut(s) 838, 1339, 2101, 2229, 2434, 2509
BstEII GGTNACC 1 cut(s) 2306
BstHHI GCGC 2 cut(s) 576, 1077
BstMAI GTCTC 4 cut(s) 1060, 2342, 2603, 2647
BstMCI CGRYCG 1 cut(s) 1905
BstMWI GCNNNNNNNGC 5 cut(s) 180, 571, 1121, 1370, 1938
BstNI CCWGG 1 cut(s) 2290
BstNSI RCATGY 1 cut(s) 1443
BstPI GGTNACC 1 cut(s) 2306
BstSCI CCNGG 2 cut(s) 10, 2288
BstSFI CTRYAG 2 cut(s) 162, 994
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 902, 2448
BstV2I GAAGAC 2 cut(s) 1152, 1667
BstX2I RGATCY 4 cut(s) 1556, 2083, 2392, 2546
BstXI CCANNNNNNTGG 1 cut(s) 1333
BstYI RGATCY 4 cut(s) 1556, 2083, 2392, 2546
BstZ17I GTATAC 1 cut(s) 41
Bsu15I ATCGAT 1 cut(s) 1901
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 115, 790, 2231
BsuTUI ATCGAT 1 cut(s) 1901
BtrI CACGTC 2 cut(s) 1166, 1293
BtsIMutI CAGTG 7 cut(s) 745, 915, 1295, 2013, 2367, 2419, 2455
Cac8I GCNNGC 6 cut(s) 838, 1339, 2101, 2229, 2434, 2509
CaiI CAGNNNCTG 1 cut(s) 1364
CfoI GCGC 2 cut(s) 576, 1077
Cfr13I GGNCC 3 cut(s) 1027, 2061, 2417
ClaI ATCGAT 1 cut(s) 1901
Csp6I GTAC 8 cut(s) 6, 82, 318, 975, 1408, 1717, 1742, 2198
CspCI CAANNNNNGTGG 6 cut(s) 411, 446, 513, 548, 2652, 2687
CviAII CATG 5 cut(s) 293, 437, 1440, 2045, 2668
CviQI GTAC 8 cut(s) 6, 82, 318, 975, 1408, 1717, 1742, 2198
DraIII CACNNNGTG 1 cut(s) 14
Eam1104I CTCTTC 3 cut(s) 1221, 1248, 1491
EarI CTCTTC 3 cut(s) 1221, 1248, 1491
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 2342
Eco47I GGWCC 3 cut(s) 1027, 2061, 2417
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 3 cut(s) 972, 1386, 1596
Eco91I GGTNACC 1 cut(s) 2306
EcoICRI GAGCTC 1 cut(s) 69
EcoO65I GGTNACC 1 cut(s) 2306
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 2288
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 5 cut(s) 296, 440, 1443, 2048, 2671
FaqI GGGAC 1 cut(s) 1402
FatI CATG 5 cut(s) 292, 436, 1439, 2044, 2667
FauI CCCGC 2 cut(s) 2096, 2684
FauNDI CATATG 2 cut(s) 190, 879
FbaI TGATCA 1 cut(s) 1525
FblI GTMKAC 2 cut(s) 40, 248
Fnu4HI GCNGC 3 cut(s) 891, 2437, 2679
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 3 cut(s) 891, 2437, 2679
FspBI CTAG 6 cut(s) 771, 804, 828, 837, 1491, 2280
GlaI GCGC 2 cut(s) 575, 1076
GluI GCNGC 3 cut(s) 891, 2437, 2679
GsaI CCCAGC 1 cut(s) 1830
GsuI CTGGAG 2 cut(s) 2152, 2671
HaeIII GGCC 3 cut(s) 115, 790, 2231
HapII CCGG 3 cut(s) 11, 202, 2601
HhaI GCGC 2 cut(s) 576, 1077
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 5 cut(s) 296, 440, 1443, 2048, 2671
Hin6I GCGC 2 cut(s) 574, 1075
HinP1I GCGC 2 cut(s) 574, 1075
HincII GTYRAC 2 cut(s) 1512, 2068
HindII GTYRAC 2 cut(s) 1512, 2068
HindIII AAGCTT 1 cut(s) 1449
HpaI GTTAAC 1 cut(s) 1512
HpaII CCGG 3 cut(s) 11, 202, 2601
HphI GGTGA 5 cut(s) 29, 293, 588, 722, 2300
Hpy166II GTNNAC 6 cut(s) 8, 41, 249, 1512, 2068, 2198
Hpy8I GTNNAC 6 cut(s) 8, 41, 249, 1512, 2068, 2198
HpyAV CCTTC 7 cut(s) 250, 399, 588, 609, 959, 1309, 1979
HpyCH4IV ACGT 4 cut(s) 475, 820, 1165, 1292
HpyF10VI GCNNNNNNNGC 5 cut(s) 180, 571, 1121, 1370, 1938
HpySE526I ACGT 4 cut(s) 475, 820, 1165, 1292
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 5 cut(s) 296, 440, 1443, 2048, 2671
HspAI GCGC 2 cut(s) 574, 1075
Kpn2I TCCGGA 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 1525
KspAI GTTAAC 1 cut(s) 1512
LmnI GCTCC 4 cut(s) 969, 1682, 2133, 2588
Lsp1109I GCAGC 2 cut(s) 902, 2448
LweI GCATC 6 cut(s) 361, 1247, 1249, 1309, 1351, 2372
MaeI CTAG 6 cut(s) 771, 804, 828, 837, 1491, 2280
MaeII ACGT 4 cut(s) 475, 820, 1165, 1292
MaeIII GTNAC 7 cut(s) 17, 325, 594, 1140, 1293, 1766, 2306
MfeI CAATTG 3 cut(s) 55, 923, 1676
MflI RGATCY 4 cut(s) 1556, 2083, 2392, 2546
MhlI GDGCHC 2 cut(s) 71, 417
MlyI GAGTC 5 cut(s) 1031, 1424, 1511, 1657, 2336
MmeI TCCRAC 3 cut(s) 28, 841, 1193
MroI TCCGGA 1 cut(s) 201
MslI CAYNNNNRTG 2 cut(s) 2460, 2702
MspA1I CMGCKG 1 cut(s) 2103
MspCI CTTAAG 1 cut(s) 451
MspI CCGG 3 cut(s) 11, 202, 2601
MspR9I CCNGG 2 cut(s) 12, 2290
MunI CAATTG 3 cut(s) 55, 923, 1676
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 1 cut(s) 2290
MwoI GCNNNNNNNGC 5 cut(s) 180, 571, 1121, 1370, 1938
NciI CCSGG 1 cut(s) 12
NdeI CATATG 2 cut(s) 190, 879
NheI GCTAGC 1 cut(s) 836
NlaIII CATG 5 cut(s) 296, 440, 1443, 2048, 2671
NlaIV GGNNCC 3 cut(s) 736, 2161, 2584
NmuCI GTSAC 4 cut(s) 17, 594, 1293, 2306
NspI RCATGY 1 cut(s) 1443
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 7 cut(s) 299, 353, 1564, 1640, 1877, 1919, 2591
PkrI GCNGC 3 cut(s) 892, 2438, 2680
Ple19I CGATCG 1 cut(s) 1905
PleI GAGTC 5 cut(s) 1031, 1423, 1510, 1657, 2336
PpsI GAGTC 5 cut(s) 1031, 1423, 1510, 1657, 2336
PshBI ATTAAT 2 cut(s) 2139, 2622
Psp124BI GAGCTC 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 2288
PspEI GGTNACC 1 cut(s) 2306
PspFI CCCAGC 1 cut(s) 1826
PspGI CCWGG 1 cut(s) 2288
PspN4I GGNNCC 3 cut(s) 736, 2161, 2584
PspPI GGNCC 3 cut(s) 1027, 2061, 2417
PstNI CAGNNNCTG 1 cut(s) 1364
PsuI RGATCY 4 cut(s) 1556, 2083, 2392, 2546
PvuI CGATCG 1 cut(s) 1905
RsaI GTAC 8 cut(s) 7, 83, 319, 976, 1409, 1718, 1743, 2199
RsaNI GTAC 8 cut(s) 6, 82, 318, 975, 1408, 1717, 1742, 2198
RseI CAYNNNNRTG 2 cut(s) 2460, 2702
SacI GAGCTC 1 cut(s) 71
SatI GCNGC 3 cut(s) 891, 2437, 2679
Sau96I GGNCC 3 cut(s) 1027, 2061, 2417
ScaI AGTACT 1 cut(s) 1743
SchI GAGTC 5 cut(s) 1031, 1424, 1511, 1657, 2336
ScrFI CCNGG 2 cut(s) 12, 2290
SduI GDGCHC 2 cut(s) 71, 417
SfaNI GCATC 6 cut(s) 361, 1247, 1249, 1309, 1351, 2372
SfcI CTRYAG 2 cut(s) 162, 994
SinI GGWCC 3 cut(s) 1027, 2061, 2417
SmiMI CAYNNNNRTG 2 cut(s) 2460, 2702
SmlI CTYRAG 5 cut(s) 70, 451, 549, 1532, 2076
SmoI CTYRAG 5 cut(s) 70, 451, 549, 1532, 2076
SpeI ACTAGT 1 cut(s) 803
SsiI CCGC 7 cut(s) 146, 288, 2103, 2243, 2511, 2679, 2691
SspI AATATT 1 cut(s) 1522
SspMI CTAG 6 cut(s) 771, 804, 828, 837, 1491, 2280
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 10, 2288
StyI CCWWGG 1 cut(s) 429
TaiI ACGT 4 cut(s) 478, 823, 1168, 1295
TaqI TCGA 4 cut(s) 1251, 1901, 1905, 2032
TatI WGTACW 4 cut(s) 317, 974, 1741, 2197
TauI GCSGC 1 cut(s) 2681
TfiI GAWTC 7 cut(s) 299, 353, 1564, 1640, 1877, 1919, 2591
TscAI CASTG 7 cut(s) 745, 922, 1302, 2020, 2374, 2419, 2455
TseFI GTSAC 4 cut(s) 17, 594, 1293, 2306
TseI GCWGC 2 cut(s) 890, 2436
Tsp45I GTSAC 4 cut(s) 17, 594, 1293, 2306
TspDTI ATGAA 9 cut(s) 177, 456, 978, 1136, 1880, 1932, 1998, 2061, 2559
TspGWI ACGGA 3 cut(s) 1019, 2053, 2476
TspRI CASTG 7 cut(s) 745, 922, 1302, 2020, 2374, 2419, 2455
Vha464I CTTAAG 1 cut(s) 451
VpaK11BI GGWCC 3 cut(s) 1027, 2061, 2417
VspI ATTAAT 2 cut(s) 2139, 2622
XapI RAATTY 9 cut(s) 233, 719, 1083, 1096, 1182, 1633, 1774, 2333, 2576
XceI RCATGY 1 cut(s) 1443
XcmI CCANNNNNNNNNTGG 1 cut(s) 2224
XmiI GTMKAC 2 cut(s) 40, 248
XspI CTAG 6 cut(s) 771, 804, 828, 837, 1491, 2280
ZraI GACGTC 1 cut(s) 476
ZrmI AGTACT 1 cut(s) 1743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.