Rmu_ssc0000122.1_g000011

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000122.1
OG0009426
Physical Location & Seq
Reverse (-)
51449 .. 61078
9630 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4812 bp
atggatcaaaaccagagatcgtcgtcggcctcagcttcgcgagcttaccagttccaccctgctcgcgctgcaatcgtcaatctcttcgacctctacttaggaggaaagagcagtagtcgccagaaatcagaggattcaaatcgcgaacctccgaacaagtcacagaagcgtgtgcttgcgctcaatagagaacttcctcctcgaaacgagcagttccttctcgatttcgagcagctccagagccagtttcctgaccaagatcaactgcgtgttgtgacagagtctgtgcttatatctctagttgtgcagtgcagtaatcatgcaccacgggcagagtttcttctctttgctttaaggagtttgtgcacaataggtcacattaactgggatagttttttgccgtcccttctttcttctgtctccacggcagaaatgtcaatgggtcaagggagtagtcaagcaatgacgggtatttcatcacagtccagtatgcttccaagttcaaatgcaattcccaattcatctaactttcaatcttcaaatcctgcctctccattaccttctgttcatggaattggttcccccagccagacagctattgaacctatgactctgtcacctgctaaatcatctgatatgcccagtaacggacagcaggctgcagcaagggccaattcatctataagagataatgcaatcagcagtctacgtcagttgtgttgcaaaattattttgactggactggaatttaatttaaaaccagttactcatgcggacatattttctcatatgctgaattggctggttaattgggatcaaaaacaactgggggctgatgaatctgatggcataaagtcttggagacctgggaaggcactaattgaatggctgcatagctgtttggatgtcatttggcttttggttgacgaggataaatgtcgagttcccttctatgaactactgcgcagtggcttgcaattcatggagaacatacctgatgatgaagctttgtttactctaatcttggagatccataggaggcgagatatgatggctatgcacatgaaaatgctagatcaacacttacattgccctacatttgggactcatcgaattttctcgcagactactcctagtatttcaggtgaagcagtggcaagcttgcggtattcaccaatcacatatccaagtgtacttggggaaccattgcatggagaggatcttgcaatttctattccaaaaggaagtttagattgggagagagcgttgcgttgcataaggcatgctatttgtactactccatcacctgactggtggaaacgtgtgctgcttgtggctccttgttataggggtccttctcaaggacctacccctggtgctgttttcacttctgaaatgatttgcgaggcaacaattgatagaattgttgagctactgaagttgacaaattcagatgttaattgctggcaagactggcttgtcttttcagatatattcttttttcttataaaaagcggctgtgttgatttcgtctattttgtggacaagctagtttcacgccttacagagagtgatccctatattcttaagaccaatcatgtcacttggcttctagctcaaattattcgggttgagcttgtgataaatgctctaaattcagatgctagaaaggtagagacaactcggaagatcctatcgcttcataaagaagatagaagctcagatccaaatagtcctcagagtatcctgcttgactttataagcagttgccaaaatttacgtatttggtcattgaatacaacaactagagaatatctgaataatgagcagcttcagaaaggaaagcaaatagatgaatggtggagacatgcaagcaaaggggatcgtatgatggattatatgaatatggatgataggtcaattgggatgttttgggttgtatcttacactatggcgcaaccagcttgtgaaactgtgatcaattggttatcctctactggagttgcagaatcgttaccggcaacaaacctacagtccaatgagaggttaatggttatgcgggaagtcaatccattgccaatgtcattgttatctggctttgcaataaacctgtgtttaaaactggcatatcaaatggaagaagctttgttttgcgggcaggttgtacctaatattgcaatggctgaaacatattgcaggttactgctcattgcacctcattcactattccgttctcatttcaaccatttggcacaaaggagtccttcagtattgagcaagcccggcgttactcttttggtgcttgaaattttgaactatcgtttgcttccactttacaggtaccagggaaaaagcaaagccttgatgtatgatgtaacaaaaataatctctgctctgaaagcaaaacgtggagatcatcgtgtatttagattggccgagaacttgtgcatgaatcttattctgtcactgagagatttcttcttagtgaaaagggaagggaagggtccaactgaattcacagaaactttgaatcgagtaactgtggtcactcttgctatcatcatcaagacaagaggaattgctgacgctgatcatctgcattatcttcaaactatgttagagcagatattagtttctagtaatcatacatggtcagagaaaacactacgctatttcccttcgcttcttcgtgatttcttgaatcttcggatagataacaggggcattgcaattcaagcatggcagcaggcagaaaccactgtgattaaccaatgcaaccagcttctgtcatcatcagctgatcctacttatgttatgacctatataagtcatagttttcctcaacaccgacaatatctatgtgctggagcatggatcctgatgcaagggcgcccagagaatataaatagtgtaaacctggcacgtgtcttgcgagagttttcccctgaggaagtaactgctaatatttacatgatggtggatgtgctactccataacattcagttagaacatcagcgcgggcattccttgcaggaccttctagtgaaagcatgtgcaaacctaacattttttatttggacccatgagttacttcctttggatattatgcttctggcacttattgatcgggatgatgatccccatgctttgcgtattgtgattagtttacttgataggcaagaacttcaacaaagagtaaaactatattgtatgaatcgaggcgcccctgaacactggctttaccctggaaccttcaaacgagttgaattgcagacagcccttggaaatcatctttcttggaaggataggtatcccgcattcttcgatgacattgcagcgcgtttgcttccggtcattccactaataatttatagactaattgaaaatgatgccgtagattcagcggacagagttctggccatgtatactcaatttcttgcttaccacccttttagatttacatttgttcgtgacattcttgcatatttctacggccatcttcccccgaagcttattgttcgaattctgaatgtacttgatatcagcaagatcccattgtctgagtcatttccgcagcacgtcaattcatcaaatcctgtcatctgcccacctccagattactttgcaactctcctactgggaattgtgaataatgttatacctccattacataacaactcaaaatctggatcagctagtgatggtttaaataattcaatgcgtgctccacctaacaagactccagcaacatctcaatctgggcaaacaaatgcttctgatggccaaaaagcattttatcaaattcaggatccaggcacatatactcagctggttcttgaaacagcagtcattgaattactgtctcttcccgtatctgcatcccagattgtatcatctctggttcaaattgtcgtcaatatacaaccgacgctaattcaatccagcaatggcctgcatggagctacaaatggtgttgggcaaggctcagtgttaccaacatccccttcaggtggaagcacagattccttgggcacaaacagatcaagtccttcagtttctggaataaatgtttctagctttgtttctcgaagtggttatacatgccaacaattgtcttgcttattgatccaagcatgtggccatctactggctcagctccctcctgattttcataaacaactctatatagaggcatcacgtataataaaagaaacatggtggctcagtgatggcaagagatcactgggggaactagactctgctgttggttatgctttgttggacccaacatgggctgctcaagacaacacctcaacagccattggtaatattgttgctctgcttcactcatttttcagtaacctcccactggaatggctagaagggacccatcttatcgtcaaacatctcaggccagtgacatcagttgcaatgttgagaatagcattccgtataatggctccgttgctcccaaagcttgccaatgctcacaacctcttcaataagaccctctcattgattttcaatatgatggcggatgtctttgggaagaatgctcagccatcaactcctgttgagccactagaagtgacagatcttatagacttcttccatcatatcgtccactatgaaggacaaggagggcctgtgcaggctaccagcaagccgagaccagaggttttagttctgtgtggaagagctgccgaaagtctacgtccagagattcagcatcttctttcgcacttaaaacccgacacaaattcttccatttatgctgctactcatcctaagttggcacagaacacatcgtaa

Protein Analysis

1603

Amino Acids

179.62

Weight (kDa)

6.7

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 492 - 1212 3.9e-27 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1508, 1760
AarI CACCTGC 1 cut(s) 628
AasI GACNNNNNNGTC 1 cut(s) 1478
Acc16I TGCGCA 1 cut(s) 965
Acc36I ACCTGC 3 cut(s) 628, 2149, 2187
Acc65I GGTACC 1 cut(s) 2340
AccB1I GGYRCC 3 cut(s) 2340, 2889, 3221
AccB7I CCANNNNNTGG 2 cut(s) 1211, 4147
AccI GTMKAC 3 cut(s) 706, 3425, 4711
AccII CGCG 5 cut(s) 40, 66, 144, 3018, 3340
AcoI YGGCCR 5 cut(s) 2433, 3417, 3493, 3781, 4138
AcuI CTGAAG 5 cut(s) 1457, 1817, 2250, 3990, 4035
AcvI CACGTG 1 cut(s) 2924
AcyI GRCGYC 2 cut(s) 2890, 3222
AfaI GTAC 5 cut(s) 1194, 1294, 2166, 2342, 3534
AflII CTTAAG 1 cut(s) 1586
AflIII ACRYGT 2 cut(s) 1321, 2923
AjiI CACGTC 1 cut(s) 3580
AjnI CCWGG 6 cut(s) 865, 1372, 2343, 2916, 3244, 3811
AjuI GAANNNNNNNTTGG 4 cut(s) 1237, 1269, 3988, 4020
Alw21I GWGCWC 2 cut(s) 368, 3727
Alw26I GTCTC 6 cut(s) 424, 856, 1670, 1858, 3867, 4663
Alw44I GTGCAC 1 cut(s) 364
AlwNI CAGNNNCTG 3 cut(s) 284, 2785, 4058
ApaLI GTGCAC 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 2018, 2050
Asp700I GAANNNNTTC 1 cut(s) 577
Asp718I GGTACC 1 cut(s) 2340
AspLEI GCGC 8 cut(s) 68, 181, 966, 1957, 2892, 3018, 3224, 3340
AspS9I GGNCC 9 cut(s) 669, 1352, 1364, 2504, 3034, 3078, 4282, 4386, 4643
AsuC2I CCSGG 1 cut(s) 2283
AsuHPI GGTGA 4 cut(s) 609, 1157, 1164, 1296
AsuII TTCGAA 1 cut(s) 3520
AvaII GGWCC 7 cut(s) 1352, 1364, 2504, 3034, 3078, 4282, 4386
AxyI CCTNAGG 1 cut(s) 2946
BaeGI GKGCMC 2 cut(s) 368, 4034
BalI TGGCCA 3 cut(s) 3419, 3783, 4140
BamHI GGATCC 2 cut(s) 2874, 3808
BanI GGYRCC 3 cut(s) 2340, 2889, 3221
BarI GAAGNNNNNNTAC 2 cut(s) 4081, 4113
BbrPI CACGTG 1 cut(s) 2924
Bbv12I GWGCWC 2 cut(s) 368, 3727
BbvCI CCTCAGC 1 cut(s) 31
BceAI ACGGC 4 cut(s) 385, 441, 3377, 3508
BcgI CGANNNNNNTGC 2 cut(s) 3314, 3348
BciT130I CCWGG 6 cut(s) 867, 1374, 2345, 2918, 3246, 3813
BciVI GTATCC 2 cut(s) 1754, 3321
BclI TGATCA 2 cut(s) 1977, 2590
BcnI CCSGG 1 cut(s) 2283
BcoDI GTCTC 6 cut(s) 424, 856, 1670, 1858, 3867, 4663
BfmI CTRYAG 2 cut(s) 660, 2030
BfoI RGCGCY 2 cut(s) 2893, 3225
BfrI CTTAAG 1 cut(s) 1586
BfuAI ACCTGC 3 cut(s) 628, 2149, 2187
BfuI GTATCC 2 cut(s) 1754, 3321
BglII AGATCT 1 cut(s) 4594
BlpI GCTNAGC 2 cut(s) 4152, 4557
Bme1390I CCNGG 7 cut(s) 867, 1374, 2283, 2345, 2918, 3246, 3813
Bme18I GGWCC 7 cut(s) 1352, 1364, 2504, 3034, 3078, 4282, 4386
BmgBI CACGTC 1 cut(s) 3580
BmgT120I GGNCC 9 cut(s) 669, 1352, 1364, 2504, 3034, 3078, 4282, 4386, 4643
BmrFI CCNGG 7 cut(s) 867, 1374, 2283, 2345, 2918, 3246, 3813
BmrI ACTGGG 5 cut(s) 394, 636, 836, 3647, 4253
BmsI GCATC 6 cut(s) 1651, 2871, 3379, 3887, 4202, 4738
BmuI ACTGGG 5 cut(s) 394, 636, 836, 3647, 4253
BpmI CTGGAG 5 cut(s) 221, 2019, 2886, 3597, 3726
Bpu10I CCTNAGC 1 cut(s) 31
Bpu1102I GCTNAGC 2 cut(s) 4152, 4557
Bpu14I TTCGAA 1 cut(s) 3520
BpuEI CTTGAG 2 cut(s) 1344, 4284
BpuMI CCSGG 1 cut(s) 2283
BsaAI YACGTR 3 cut(s) 1781, 2924, 4199
BsaBI GATNNNNATC 3 cut(s) 138, 3135, 3309
BsaHI GRCGYC 2 cut(s) 2890, 3222
BsaI GGTCTC 2 cut(s) 856, 4663
BsaJI CCNNGG 8 cut(s) 326, 423, 866, 1372, 2344, 3244, 3280, 4026
BsaWI WCCGGW 1 cut(s) 3349
BsaXI ACNNNNNCTCC 4 cut(s) 1250, 1280, 4632, 4662
Bse118I RCCGGY 1 cut(s) 2017
Bse21I CCTNAGG 1 cut(s) 2946
Bse8I GATNNNNATC 3 cut(s) 138, 3135, 3309
BseBI CCWGG 6 cut(s) 867, 1374, 2345, 2918, 3246, 3813
BseDI CCNNGG 8 cut(s) 326, 423, 866, 1372, 2344, 3244, 3280, 4026
BseGI GGATG 9 cut(s) 910, 1915, 1932, 2986, 3136, 3878, 3998, 4543, 4783
BseJI GATNNNNATC 3 cut(s) 138, 3135, 3309
BseRI GAGGAG 1 cut(s) 189
BseSI GKGCMC 2 cut(s) 368, 4034
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 3 cut(s) 326, 331, 4670
Bsh1236I CGCG 5 cut(s) 40, 66, 144, 3018, 3340
BshNI GGYRCC 3 cut(s) 2340, 2889, 3221
BsiHKAI GWGCWC 2 cut(s) 368, 3727
BsiSI CCGG 3 cut(s) 2018, 2283, 3350
BslFI GGGAC 3 cut(s) 388, 1117, 4399
BsmAI GTCTC 6 cut(s) 424, 856, 1670, 1858, 3867, 4663
BsmFI GGGAC 3 cut(s) 388, 1117, 4399
BsmI GAATGC 4 cut(s) 3022, 3317, 4445, 4558
Bso31I GGTCTC 2 cut(s) 856, 4663
Bsp119I TTCGAA 1 cut(s) 3520
Bsp1286I GDGCHC 3 cut(s) 368, 3727, 4034
Bsp1720I GCTNAGC 2 cut(s) 4152, 4557
Bsp68I TCGCGA 2 cut(s) 40, 144
BspFNI CGCG 5 cut(s) 40, 66, 144, 3018, 3340
BspMAI CTGCAG 1 cut(s) 664
BspMI ACCTGC 3 cut(s) 628, 2149, 2187
BspQI GCTCTTC 1 cut(s) 4690
BspT104I TTCGAA 1 cut(s) 3520
BspT107I GGYRCC 3 cut(s) 2340, 2889, 3221
BspTI CTTAAG 1 cut(s) 1586
BspTNI GGTCTC 2 cut(s) 856, 4663
BsrFI RCCGGY 1 cut(s) 2017
BssAI RCCGGY 1 cut(s) 2017
BssECI CCNNGG 8 cut(s) 326, 423, 866, 1372, 2344, 3244, 3280, 4026
BssNAI GTATAC 1 cut(s) 3426
BssNI GRCGYC 2 cut(s) 2890, 3222
BssT1I CCWWGG 2 cut(s) 3280, 4026
Bst1107I GTATAC 1 cut(s) 3426
Bst2UI CCWGG 6 cut(s) 867, 1374, 2345, 2918, 3246, 3813
Bst4CI ACNGT 6 cut(s) 483, 1975, 2034, 2542, 2761, 3861
Bst6I CTCTTC 4 cut(s) 89, 3870, 4502, 4690
BstACI GRCGYC 2 cut(s) 2890, 3222
BstAFI CTTAAG 1 cut(s) 1586
BstAPI GCANNNNNTGC 1 cut(s) 3028
BstBAI YACGTR 3 cut(s) 1781, 2924, 4199
BstBI TTCGAA 1 cut(s) 3520
BstDSI CCRYGG 2 cut(s) 326, 423
BstENI CCTNNNNNAGG 1 cut(s) 1359
BstF5I GGATG 9 cut(s) 910, 1915, 1932, 2986, 3136, 3878, 3998, 4543, 4783
BstFNI CGCG 5 cut(s) 40, 66, 144, 3018, 3340
BstH2I RGCGCY 2 cut(s) 2893, 3225
BstHHI GCGC 8 cut(s) 68, 181, 966, 1957, 2892, 3018, 3224, 3340
BstMAI GTCTC 6 cut(s) 424, 856, 1670, 1858, 3867, 4663
BstNI CCWGG 6 cut(s) 867, 1374, 2345, 2918, 3246, 3813
BstNSI RCATGY 5 cut(s) 1286, 1871, 3054, 4104, 4137
BstSCI CCNGG 7 cut(s) 865, 1372, 2281, 2343, 2916, 3244, 3811
BstSFI CTRYAG 2 cut(s) 660, 2030
BstSLI GKGCMC 2 cut(s) 368, 4034
BstSNI TACGTA 1 cut(s) 1781
BstUI CGCG 5 cut(s) 40, 66, 144, 3018, 3340
BstX2I RGATCY 8 cut(s) 1029, 1219, 1689, 1723, 2874, 3549, 3808, 4594
BstXI CCANNNNNNTGG 2 cut(s) 4136, 4374
BstYI RGATCY 8 cut(s) 1029, 1219, 1689, 1723, 2874, 3549, 3808, 4594
BstZ17I GTATAC 1 cut(s) 3426
Bsu36I CCTNAGG 1 cut(s) 2946
BsuI GTATCC 2 cut(s) 1754, 3321
BtgI CCRYGG 2 cut(s) 326, 423
BtrI CACGTC 1 cut(s) 3580
BtsCI GGATG 9 cut(s) 910, 1915, 1932, 2986, 3136, 3878, 3998, 4543, 4783
BtsI GCAGTG 3 cut(s) 314, 973, 1158
BtuMI TCGCGA 2 cut(s) 40, 144
BveI ACCTGC 3 cut(s) 628, 2149, 2187
CaiI CAGNNNCTG 3 cut(s) 284, 2785, 4058
CfoI GCGC 8 cut(s) 68, 181, 966, 1957, 2892, 3018, 3224, 3340
Cfr10I RCCGGY 1 cut(s) 2017
Cfr13I GGNCC 9 cut(s) 669, 1352, 1364, 2504, 3034, 3078, 4282, 4386, 4643
CseI GACGC 2 cut(s) 2594, 3937
Csp6I GTAC 5 cut(s) 1193, 1293, 2165, 2341, 3533
CviQI GTAC 5 cut(s) 1193, 1293, 2165, 2341, 3533
DinI GGCGCC 2 cut(s) 2891, 3223
DraI TTTAAA 3 cut(s) 756, 2118, 3708
DrdI GACNNNNNNGTC 1 cut(s) 1478
DseDI GACNNNNNNGTC 1 cut(s) 1478
EaeI YGGCCR 5 cut(s) 2433, 3417, 3493, 3781, 4138
Eam1104I CTCTTC 4 cut(s) 89, 3870, 4502, 4690
EarI CTCTTC 4 cut(s) 89, 3870, 4502, 4690
EciI GGCGGA 1 cut(s) 4550
Eco105I TACGTA 1 cut(s) 1781
Eco130I CCWWGG 2 cut(s) 3280, 4026
Eco31I GGTCTC 2 cut(s) 856, 4663
Eco32I GATATC 1 cut(s) 3541
Eco47I GGWCC 7 cut(s) 1352, 1364, 2504, 3034, 3078, 4282, 4386
Eco57I CTGAAG 5 cut(s) 1457, 1817, 2250, 3990, 4035
Eco72I CACGTG 1 cut(s) 2924
Eco81I CCTNAGG 1 cut(s) 2946
EcoNI CCTNNNNNAGG 1 cut(s) 1359
EcoO109I RGGNCCY 5 cut(s) 1352, 1364, 3034, 4386, 4643
EcoRI GAATTC 2 cut(s) 2513, 3522
EcoRII CCWGG 6 cut(s) 865, 1372, 2343, 2916, 3244, 3811
EcoRV GATATC 1 cut(s) 3541
EcoT14I CCWWGG 2 cut(s) 3280, 4026
EgeI GGCGCC 2 cut(s) 2891, 3223
EheI GGCGCC 2 cut(s) 2891, 3223
ErhI CCWWGG 2 cut(s) 3280, 4026
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 2248, 2280
FaqI GGGAC 3 cut(s) 388, 1117, 4399
FauI CCCGC 4 cut(s) 2052, 2147, 3011, 3322
FauNDI CATATG 1 cut(s) 789
FbaI TGATCA 2 cut(s) 1977, 2590
FblI GTMKAC 3 cut(s) 706, 3425, 4711
FokI GGATG 9 cut(s) 917, 1922, 1939, 2993, 3143, 3865, 3985, 4550, 4770
FspI TGCGCA 1 cut(s) 965
GlaI GCGC 8 cut(s) 67, 180, 965, 1956, 2891, 3017, 3223, 3339
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 5 cut(s) 221, 2019, 2886, 3597, 3726
HaeII RGCGCY 2 cut(s) 2893, 3225
HapII CCGG 3 cut(s) 2018, 2283, 3350
HgaI GACGC 2 cut(s) 2594, 3937
HhaI GCGC 8 cut(s) 68, 181, 966, 1957, 2892, 3018, 3224, 3340
Hin1I GRCGYC 2 cut(s) 2890, 3222
Hin6I GCGC 8 cut(s) 66, 179, 964, 1955, 2890, 3016, 3222, 3338
HinP1I GCGC 8 cut(s) 66, 179, 964, 1955, 2890, 3016, 3222, 3338
HincII GTYRAC 2 cut(s) 925, 1443
HindII GTYRAC 2 cut(s) 925, 1443
HindIII AAGCTT 5 cut(s) 1005, 1159, 2142, 3509, 4475
HpaII CCGG 3 cut(s) 2018, 2283, 3350
HphI GGTGA 4 cut(s) 609, 1157, 1164, 1296
Hpy99I CGWCG 3 cut(s) 25, 28, 3931
HpyCH4III ACNGT 6 cut(s) 483, 1975, 2034, 2542, 2761, 3861
HpyCH4IV ACGT 8 cut(s) 709, 1321, 1780, 2407, 2923, 3579, 4198, 4714
HpySE526I ACGT 8 cut(s) 709, 1321, 1780, 2407, 2923, 3579, 4198, 4714
Hsp92I GRCGYC 2 cut(s) 2890, 3222
HspAI GCGC 8 cut(s) 66, 179, 964, 1955, 2890, 3016, 3222, 3338
KasI GGCGCC 2 cut(s) 2889, 3221
KflI GGGWCCC 1 cut(s) 4386
KpnI GGTACC 1 cut(s) 2344
Ksp22I TGATCA 2 cut(s) 1977, 2590
LguI GCTCTTC 1 cut(s) 4690
LmnI GCTCC 8 cut(s) 240, 1342, 2867, 3730, 3959, 4161, 4465, 4473
LweI GCATC 6 cut(s) 1651, 2871, 3379, 3887, 4202, 4738
MaeII ACGT 8 cut(s) 709, 1321, 1780, 2407, 2923, 3579, 4198, 4714
MfeI CAATTG 4 cut(s) 1413, 1920, 1981, 4109
MflI RGATCY 8 cut(s) 1029, 1219, 1689, 1723, 2874, 3549, 3808, 4594
MhlI GDGCHC 3 cut(s) 368, 3727, 4034
MlsI TGGCCA 3 cut(s) 3419, 3783, 4140
MluNI TGGCCA 3 cut(s) 3419, 3783, 4140
Mly113I GGCGCC 2 cut(s) 2890, 3222
MlyI GAGTC 7 cut(s) 290, 604, 1099, 2269, 3572, 3733, 4250
MmeI TCCRAC 2 cut(s) 2531, 4260
Mox20I TGGCCA 3 cut(s) 3419, 3783, 4140
MroXI GAANNNNTTC 1 cut(s) 577
MscI TGGCCA 3 cut(s) 3419, 3783, 4140
MslI CAYNNNNRTG 3 cut(s) 1067, 2975, 4372
Msp20I TGGCCA 3 cut(s) 3419, 3783, 4140
MspA1I CMGCKG 3 cut(s) 2798, 3404, 3829
MspCI CTTAAG 1 cut(s) 1586
MspI CCGG 3 cut(s) 2018, 2283, 3350
MspR9I CCNGG 7 cut(s) 867, 1374, 2283, 2345, 2918, 3246, 3813
MunI CAATTG 4 cut(s) 1413, 1920, 1981, 4109
Mva1269I GAATGC 4 cut(s) 3022, 3317, 4445, 4558
MvaI CCWGG 6 cut(s) 867, 1374, 2345, 2918, 3246, 3813
MvnI CGCG 5 cut(s) 40, 66, 144, 3018, 3340
NarI GGCGCC 2 cut(s) 2890, 3222
NciI CCSGG 1 cut(s) 2283
NdeI CATATG 1 cut(s) 789
NmeAIII GCCGAG 2 cut(s) 2461, 4692
NruI TCGCGA 2 cut(s) 40, 144
NsbI TGCGCA 1 cut(s) 965
NspI RCATGY 5 cut(s) 1286, 1871, 3054, 4104, 4137
NspV TTCGAA 1 cut(s) 3520
PaeI GCATGC 1 cut(s) 1286
PaqCI CACCTGC 1 cut(s) 628
PciSI GCTCTTC 1 cut(s) 4690
PcsI WCGNNNNNNNCGW 1 cut(s) 2929
PctI GAATGC 4 cut(s) 3022, 3317, 4445, 4558
PdmI GAANNNNTTC 1 cut(s) 577
PflFI GACNNNGTC 2 cut(s) 280, 613
PflMI CCANNNNNTGG 2 cut(s) 1211, 4147
PleI GAGTC 7 cut(s) 289, 604, 1099, 2268, 3571, 3733, 4250
PluTI GGCGCC 2 cut(s) 2893, 3225
PmaCI CACGTG 1 cut(s) 2924
PmlI CACGTG 1 cut(s) 2924
PpsI GAGTC 7 cut(s) 289, 604, 1099, 2268, 3571, 3733, 4250
Ppu21I YACGTR 3 cut(s) 1781, 2924, 4199
PpuMI RGGWCCY 4 cut(s) 1352, 1364, 3034, 4386
PsiI TTATAA 2 cut(s) 1508, 1760
Psp5II RGGWCCY 4 cut(s) 1352, 1364, 3034, 4386
Psp6I CCWGG 6 cut(s) 865, 1372, 2343, 2916, 3244, 3811
PspCI CACGTG 1 cut(s) 2924
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 6 cut(s) 865, 1372, 2343, 2916, 3244, 3811
PspPI GGNCC 9 cut(s) 669, 1352, 1364, 2504, 3034, 3078, 4282, 4386, 4643
PspPPI RGGWCCY 4 cut(s) 1352, 1364, 3034, 4386
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 3 cut(s) 284, 2785, 4058
PsuI RGATCY 8 cut(s) 1029, 1219, 1689, 1723, 2874, 3549, 3808, 4594
PsyI GACNNNGTC 2 cut(s) 280, 613
PvuII CAGCTG 2 cut(s) 2798, 3829
RruI TCGCGA 2 cut(s) 40, 144
RsaI GTAC 5 cut(s) 1194, 1294, 2166, 2342, 3534
RsaNI GTAC 5 cut(s) 1193, 1293, 2165, 2341, 3533
RseI CAYNNNNRTG 3 cut(s) 1067, 2975, 4372
SapI GCTCTTC 1 cut(s) 4690
Sau96I GGNCC 9 cut(s) 669, 1352, 1364, 2504, 3034, 3078, 4282, 4386, 4643
SchI GAGTC 7 cut(s) 290, 604, 1099, 2269, 3572, 3733, 4250
ScrFI CCNGG 7 cut(s) 867, 1374, 2283, 2345, 2918, 3246, 3813
SduI GDGCHC 3 cut(s) 368, 3727, 4034
SfaNI GCATC 6 cut(s) 1651, 2871, 3379, 3887, 4202, 4738
SfcI CTRYAG 2 cut(s) 660, 2030
SfoI GGCGCC 2 cut(s) 2891, 3223
SfuI TTCGAA 1 cut(s) 3520
SinI GGWCC 7 cut(s) 1352, 1364, 2504, 3034, 3078, 4282, 4386
SmiMI CAYNNNNRTG 3 cut(s) 1067, 2975, 4372
SmlI CTYRAG 3 cut(s) 1359, 1586, 4299
SmoI CTYRAG 3 cut(s) 1359, 1586, 4299
SnaBI TACGTA 1 cut(s) 1781
SphI GCATGC 1 cut(s) 1286
SspDI GGCGCC 2 cut(s) 2889, 3221
SspI AATATT 3 cut(s) 2173, 2965, 4330
StyD4I CCNGG 7 cut(s) 865, 1372, 2281, 2343, 2916, 3244, 3811
StyI CCWWGG 2 cut(s) 3280, 4026
TaaI ACNGT 6 cut(s) 483, 1975, 2034, 2542, 2761, 3861
TaiI ACGT 8 cut(s) 712, 1324, 1783, 2410, 2926, 3582, 4201, 4717
TatI WGTACW 3 cut(s) 1192, 1292, 3532
TauI GCSGC 1 cut(s) 1518
TspGWI ACGGA 4 cut(s) 663, 2219, 4439, 4452
Tth111I GACNNNGTC 2 cut(s) 280, 613
Van91I CCANNNNNTGG 2 cut(s) 1211, 4147
Vha464I CTTAAG 1 cut(s) 1586
VneI GTGCAC 1 cut(s) 364
VpaK11BI GGWCC 7 cut(s) 1352, 1364, 2504, 3034, 3078, 4282, 4386
XagI CCTNNNNNAGG 1 cut(s) 1359
XceI RCATGY 5 cut(s) 1286, 1871, 3054, 4104, 4137
XcmI CCANNNNNNNNNTGG 1 cut(s) 1308
XmiI GTMKAC 3 cut(s) 706, 3425, 4711
XmnI GAANNNNTTC 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.