Rmu_ssc0000233.1_g000006

GPI-anchored adhesin-like protein PGA55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000233.1
OG0011156
Physical Location & Seq
Reverse (-)
20165 .. 25354
5190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3816 bp
atggcatcttcccaagtggaaatggcctctgcgccgttcggttgcgttttgagggatcacaaccgccgggaccggtgcagagagagcaatgccagagccaccataggtgttttgcagaagaatctcaagagtttggtgagggatcatctctgcattgcagtgtcttcaaactctggctccaatgacagcaactccgagaaccagggtcgaaacatggagccaaacaaggatgagaacagcaacagaaatggtgaaagagaagatggttcgggttcggttatgagcccgaggcatgctagggttcttgatcgatgggctttcgcaagacaaatagtgacgaaaccaattgagaggcagaggagagaagaggcagaggtggtttctgtgccgaactcggcgccttcttccacaaggacctcgacttctgcaaaggaagagactttggcaagaacagatagcttggctgatctcaccaaaaaaggcgtgggggcttcgtctctggttcaaatttgggagaagaggctccacaaatcagaatccctcaaaaccaattccaatggaaatgtaatatccgagaccagtaggtcagattctggagttagctgccctgagaatgagaatgagaatgagaatgtactagctcccaatgcttcaccgtccagagtttctgaagcagaaaaggcggagtcccctggtgagaaagcaggggatactattttctctattgaaaaaacaggggactctgttttggatatggaaaaagcaagggagtctattgatgaaagctatgatgttggcgctgttaccgacattgaagattctgataggatttctgtgaatagccggaatttagattctaaagaaaaagagaaagttcgagttgtggatattattagaagactgactgcacagggtccatcgtcctctttgagtgaggaaaatgctaatgataatgatcagcagtttagtagcgctcacggatcgccatgtagggatatcccccgtgagctgttacgtgatcggtgttccggggttgatcaagcagagcacagaggcctctctcatgttataagctcaccgcgcatcagagggaggcaggcatttgccgatttgcttatgcagatggagcgtgacaggcatagggaacttgacacactggtaaaccgcaaaacagtttctaagttcccacagcgaggccgaactcgaattcagtcattacttaggcttcgattgcttcaacgtggtgtggcatttgcaggtatagatgtgccaccacttccgccaaaagcatctcgtgtgaacaaaccggcgcaaggatctagcatcatgcatctcagggagaaatttcgtactagtgctgagcagggttcagcagtgcagggtgatgcacctaatccaaatagccctcataaggagatcataaagaaccaaagtgtcactgtagacaagtcctccacctccaataagcccagtgaaaacactcctaatcgagaagttaatgttatggggcagcagaaaacattaccagtactgcgtgctagtgaagatgtttgtgaagaagcgacccccagtttacacaaatcctccacctctaataagcctagtgaaagcactcctaatcgagaagttaatgttatggagcagcagaaagcatccccagtactgcgtgctagtgaagatgtttgtgaagaagccacccagagtttacagaaatcctccacctccaataagcctagtgaaagcactcctaatcgagaagttaatgttatggagcaccagaaaacatccccagtactgcgtgctagtaaagaagctatccccagtttactcaaaacctccacctccaataagcctagtgaaagccttcctaatcgagaagttaatgatatggagcagcagaaaacatccccagtactgcgtgctagtgaggatgtttgtgaagaagtgacccccagtttagaagtttgcttccaagaaactagttcagtttctcaaagcaccttgccaaatgttagtgaagatctttgtgaagtagccagcccaagtccgaaggtcacctggcaagacacaagttcagtagctagaaaccttggttcgcaagaaactgctgatccaaccacacccataaatggttcaaatgagaatgagatatggaaaggaaaaggttcagtggatagaaaccttgattcacaagaaactgtagattcaacaacatccttgaatggtcgcgatggggatgatgaaatatggaaacccacaagttcggaggctagataccttgattcacaacaaagcgcagatacaaggaaacccttgagtggtggagatgggactgggaatgaaatatggaaaggaacaagttcagaggctagaaaccttgacacacatgaagctgcagatacaacctcatccttgaacagttggaatgaaaatgcgatatggcatggaacaagtttagaggctagaaactttgactcacaagtagatacaacaacatccttggtcgactgggatgagaatgatgatgattaccagtattacggagaaacaaattatgactggatcagtgagatttctcgcccacggagctattgggaagacattaggaaatcatggtaccaagagatgcttaactccaattcagaaaaaggcgagatacaccaactccttgaaagaagaacagtatcaaactttctttctggtgatttccgagataggatagatagattgatggtatctcgcatagaaagacaaacatgtcacagtgtcagtcaaaaagaagaagatgacgagagcagctatgatagaatgaatcaaatggtgtatttcttacaacaacgcagcagcgcatcaggcactgaagaagcagaagaagtgcaagaacatttacaggtacaagaacaaaaccaagtccaggaacaagtgctagaacacgtgcaagtccaagaacaggtgcgagaacatcaagaccatgaagacgaggagatgacagaagaagaggaggagcaagaagaagcagaagaagcagaagaagtgaaagaacatgtacaggtactagaacaaaaccaagtccaagaacaagtgctagaacaggtgcaagtccaagaaaaggttcaagaacatcaagaccacgaggatggggggatgacagaagaagatgaggaagaagaaaatgttgacaactacgatgatgatgaagaatcaaggagcctcattagtggtcagtttcaagaagctagtgattattttgaccatgcatcattgcagccttcaccatctcactttacaacatggagttaccaagataatgaagttggggatgattcggaccaagccacttctccatctcctcaccaacggtcatcctttccatcttactatccaaatagtccacaactttcaccatctccatcttacaacgctaaccaccggcaacattctccctccctcagtcatccttcaatggaaatggagctcttatatgacttgagagggcatatgatacaactctatcaagagatgtctgagctaagaaaagaaataaagagctgcgtagacatgcaaatgacagtgcaacaatccatgaaccaggatgttcattcaggtcaagtggggatgaaaagaaactctagtgggttaacaaagaaaggcaattgccgcatttgcaatgaagcaaaaatcgactcacttttgtacaggtgtggacacatgtgcacctgtctcaagtgtgctcatgatttgcaatggaacagcggaaaatgtccaatctgtcgagctccgattgcagatgtcgtgaaggcatacttggattcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

1271

Amino Acids

142.55

Weight (kDa)

4.88

Isoelectric Point (pI)

57.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 1216 - 1263 3e-13 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34920
fragaria_vesca FvH4_3g13290 FvH4_3g13290
malus_domestica MD05G1243100.v1.1 MD10G1225200.v1.1
prunus_persica Prupe.4G120300_v2.0.a1 Prupe.4G120300_v2.0.a1
pyrus_communis pycom10g18910
rosa_chinensis RchiOBHm_Chr5g0021761
rosa_laevigata RLG00000032625
rosa_multiflora Rmu_ssc0000233.1_g000006
rosa_roxburghii Rroxscaffold_1G00056950
rosa_rugosa Rorug05G0069000 Rorug05G0069100 Rorug05G0069200
rosa_samantha Rh5AG157700 Rh5BG157300 Rh5CG171500 Rh5DG157600
rosa_wichuraiana Rw5G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1070
AasI GACNNNNNNGTC 2 cut(s) 583, 2757
Acc36I ACCTGC 1 cut(s) 1247
Acc65I GGTACC 1 cut(s) 2616
AccB1I GGYRCC 2 cut(s) 397, 2616
AccI GTMKAC 3 cut(s) 1443, 2505, 3555
AccII CGCG 2 cut(s) 1081, 2229
AciI CCGC 7 cut(s) 64, 683, 1079, 1165, 1280, 3658, 3753
AclWI GGATC 6 cut(s) 63, 150, 988, 1324, 2105, 2570
AcsI RAATTY 4 cut(s) 507, 847, 1206, 1343
AcuI CTGAAG 2 cut(s) 690, 2880
AcvI CACGTG 1 cut(s) 2935
AcyI GRCGYC 1 cut(s) 398
AfeI AGCGCT 1 cut(s) 973
AfiI CCNNNNNNNGG 8 cut(s) 1193, 1313, 1411, 2129, 2318, 2914, 2950, 3109
AflIII ACRYGT 4 cut(s) 2756, 2932, 3043, 3708
AgeI ACCGGT 1 cut(s) 72
AhlI ACTAGT 2 cut(s) 1352, 1979
AjnI CCWGG 5 cut(s) 201, 691, 2057, 2913, 3588
AjuI GAANNNNNNNTTGG 3 cut(s) 3096, 3128, 3788
Alw21I GWGCWC 6 cut(s) 1050, 1776, 3477, 3716, 3733, 3778
Alw26I GTCTC 4 cut(s) 431, 501, 569, 3725
Alw44I GTGCAC 1 cut(s) 3712
AlwI GGATC 6 cut(s) 63, 150, 988, 1324, 2105, 2570
AlwNI CAGNNNCTG 2 cut(s) 593, 2858
Ama87I CYCGRG 1 cut(s) 286
Aor51HI AGCGCT 1 cut(s) 973
AoxI GGCC 3 cut(s) 24, 1054, 1195
ApaLI GTGCAC 1 cut(s) 3712
ApoI RAATTY 4 cut(s) 507, 847, 1206, 1343
AsiGI ACCGGT 1 cut(s) 72
Asp700I GAANNNNTTC 4 cut(s) 1863, 2164, 2359, 3111
Asp718I GGTACC 1 cut(s) 2616
AspLEI GCGC 8 cut(s) 34, 400, 800, 974, 1083, 1312, 2297, 2849
AspS9I GGNCC 4 cut(s) 70, 414, 914, 3328
AsuC2I CCSGG 2 cut(s) 68, 1030
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 4 cut(s) 70, 414, 914, 3328
BaeGI GKGCMC 1 cut(s) 3716
BanI GGYRCC 2 cut(s) 397, 2616
BanII GRGCYC 3 cut(s) 287, 3477, 3778
BauI CACGAG 2 cut(s) 1293, 3131
BbrPI CACGTG 1 cut(s) 2935
BbsI GAAGAC 4 cut(s) 156, 904, 2604, 2982
Bbv12I GWGCWC 6 cut(s) 1050, 1776, 3477, 3716, 3733, 3778
BceAI ACGGC 1 cut(s) 19
BciT130I CCWGG 5 cut(s) 203, 693, 2059, 2915, 3590
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 2 cut(s) 955, 1036
BcnI CCSGG 2 cut(s) 68, 1030
BcoDI GTCTC 4 cut(s) 431, 501, 569, 3725
BcuI ACTAGT 2 cut(s) 1352, 1979
BfmI CTRYAG 3 cut(s) 1440, 2199, 2394
BfoI RGCGCY 3 cut(s) 401, 801, 975
BfuAI ACCTGC 1 cut(s) 1247
BfuI GTATCC 1 cut(s) 703
BglII AGATCT 1 cut(s) 2020
BlpI GCTNAGC 1 cut(s) 1359
BmcAI AGTACT 4 cut(s) 1530, 1662, 1794, 1914
Bme1390I CCNGG 7 cut(s) 68, 203, 693, 1030, 2059, 2915, 3590
Bme18I GGWCC 4 cut(s) 70, 414, 914, 3328
BmeT110I CYCGRG 1 cut(s) 286
BmgT120I GGNCC 4 cut(s) 70, 414, 914, 3328
BmiI GGNNCC 8 cut(s) 71, 178, 219, 399, 524, 915, 2618, 3209
BmrFI CCNGG 7 cut(s) 68, 203, 693, 1030, 2059, 2915, 3590
BmrI ACTGGG 9 cut(s) 1464, 1563, 1652, 1784, 1815, 1904, 1947, 2343, 2518
BmuI ACTGGG 9 cut(s) 1464, 1563, 1652, 1784, 1815, 1904, 1947, 2343, 2518
BpiI GAAGAC 4 cut(s) 156, 904, 2604, 2982
BpmI CTGGAG 1 cut(s) 615
Bpu1102I GCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 4 cut(s) 110, 2335, 3508, 3707
BpuMI CCSGG 2 cut(s) 68, 1030
Bsa29I ATCGAT 1 cut(s) 310
BsaAI YACGTR 2 cut(s) 1016, 2935
BsaBI GATNNNNATC 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 7 cut(s) 202, 287, 691, 1029, 2089, 2499, 2582
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 8 cut(s) 1193, 1313, 1411, 2129, 2318, 2914, 2950, 3109
Bse118I RCCGGY 3 cut(s) 72, 1306, 3429
Bse3DI GCAATG 5 cut(s) 94, 153, 3260, 3673, 3749
Bse8I GATNNNNATC 1 cut(s) 954
BseBI CCWGG 5 cut(s) 203, 693, 2059, 2915, 3590
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 7 cut(s) 202, 287, 691, 1029, 2089, 2499, 2582
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 8 cut(s) 1193, 1313, 1411, 2129, 2318, 2914, 2950, 3109
BseMI GCAATG 5 cut(s) 94, 153, 3260, 3673, 3749
BseMII CTCAG 5 cut(s) 600, 1348, 1350, 3463, 3516
BseRI GAGGAG 5 cut(s) 373, 2996, 3014, 3017, 3339
BseSI GKGCMC 1 cut(s) 3716
BsgI GTGCAG 3 cut(s) 97, 891, 1397
Bsh1236I CGCG 2 cut(s) 1081, 2229
BshFI GGCC 3 cut(s) 26, 1056, 1197
BshNI GGYRCC 2 cut(s) 397, 2616
BshTI ACCGGT 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 310
BsiHKAI GWGCWC 6 cut(s) 1050, 1776, 3477, 3716, 3733, 3778
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 6 cut(s) 67, 73, 844, 1029, 1307, 3430
BslFI GGGAC 4 cut(s) 83, 673, 752, 2344
BslI CCNNNNNNNGG 8 cut(s) 1193, 1313, 1411, 2129, 2318, 2914, 2950, 3109
BsmAI GTCTC 4 cut(s) 431, 501, 569, 3725
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 4 cut(s) 83, 673, 752, 2344
BsnI GGCC 3 cut(s) 26, 1056, 1197
Bso31I GGTCTC 1 cut(s) 569
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 7 cut(s) 287, 1050, 1776, 3477, 3716, 3733, 3778
Bsp1407I TGTACA 2 cut(s) 3046, 3693
Bsp1720I GCTNAGC 1 cut(s) 1359
Bsp68I TCGCGA 1 cut(s) 2229
BspACI CCGC 7 cut(s) 64, 683, 1079, 1165, 1280, 3658, 3753
BspANI GGCC 3 cut(s) 26, 1056, 1197
BspCNI CTCAG 5 cut(s) 601, 1347, 1351, 3462, 3517
BspDI ATCGAT 1 cut(s) 310
BspFNI CGCG 2 cut(s) 1081, 2229
BspHI TCATGA 1 cut(s) 3733
BspLI GGNNCC 8 cut(s) 71, 178, 219, 399, 524, 915, 2618, 3209
BspMAI CTGCAG 1 cut(s) 2398
BspMI ACCTGC 1 cut(s) 1247
BspPI GGATC 6 cut(s) 63, 150, 988, 1324, 2105, 2570
BspT107I GGYRCC 2 cut(s) 397, 2616
BspTNI GGTCTC 1 cut(s) 569
BsrDI GCAATG 5 cut(s) 94, 153, 3260, 3673, 3749
BsrFI RCCGGY 3 cut(s) 72, 1306, 3429
BsrGI TGTACA 2 cut(s) 3046, 3693
BssAI RCCGGY 3 cut(s) 72, 1306, 3429
BssECI CCNNGG 7 cut(s) 202, 287, 691, 1029, 2089, 2499, 2582
BssNI GRCGYC 1 cut(s) 398
BssSI CACGAG 2 cut(s) 1293, 3131
BssT1I CCWWGG 2 cut(s) 2089, 2499
Bst2BI CACGAG 2 cut(s) 1293, 3131
Bst2UI CCWGG 5 cut(s) 203, 693, 2059, 2915, 3590
Bst4CI ACNGT 9 cut(s) 657, 1174, 1441, 2200, 2420, 2683, 2765, 3360, 3571
Bst6I CTCTTC 4 cut(s) 360, 429, 512, 2991
BstACI GRCGYC 1 cut(s) 398
BstAUI TGTACA 2 cut(s) 3046, 3693
BstBAI YACGTR 2 cut(s) 1016, 2935
BstC8I GCNNGC 7 cut(s) 294, 1098, 1536, 1668, 1800, 1920, 2038
BstDEI CTNAG 8 cut(s) 609, 1179, 1220, 1334, 1359, 3449, 3525, 3530
BstDSI CCRYGG 1 cut(s) 2582
BstEII GGTNACC 1 cut(s) 2053
BstFNI CGCG 2 cut(s) 1081, 2229
BstH2I RGCGCY 3 cut(s) 401, 801, 975
BstHHI GCGC 8 cut(s) 34, 400, 800, 974, 1083, 1312, 2297, 2849
BstMAI GTCTC 4 cut(s) 431, 501, 569, 3725
BstNI CCWGG 5 cut(s) 203, 693, 2059, 2915, 3590
BstNSI RCATGY 5 cut(s) 296, 2760, 3047, 3562, 3712
BstPI GGTNACC 1 cut(s) 2053
BstSCI CCNGG 7 cut(s) 66, 201, 691, 1028, 2057, 2913, 3588
BstSFI CTRYAG 3 cut(s) 1440, 2199, 2394
BstSLI GKGCMC 1 cut(s) 3716
BstUI CGCG 2 cut(s) 1081, 2229
BstV2I GAAGAC 4 cut(s) 156, 904, 2604, 2982
BstX2I RGATCY 2 cut(s) 1316, 2020
BstXI CCANNNNNNTGG 1 cut(s) 3137
BstYI RGATCY 2 cut(s) 1316, 2020
Bsu15I ATCGAT 1 cut(s) 310
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 3 cut(s) 26, 1056, 1197
BsuTUI ATCGAT 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 2582
BtgZI GCGATG 1 cut(s) 2244
BtsI GCAGTG 2 cut(s) 165, 1380
BtuMI TCGCGA 1 cut(s) 2229
BveI ACCTGC 1 cut(s) 1247
Cac8I GCNNGC 7 cut(s) 294, 1098, 1536, 1668, 1800, 1920, 2038
CaiI CAGNNNCTG 2 cut(s) 593, 2858
CciI TCATGA 1 cut(s) 3733
CfoI GCGC 8 cut(s) 34, 400, 800, 974, 1083, 1312, 2297, 2849
Cfr10I RCCGGY 3 cut(s) 72, 1306, 3429
Cfr13I GGNCC 4 cut(s) 70, 414, 914, 3328
ClaI ATCGAT 1 cut(s) 310
CspAI ACCGGT 1 cut(s) 72
DdeI CTNAG 8 cut(s) 609, 1179, 1220, 1334, 1359, 3449, 3525, 3530
DinI GGCGCC 1 cut(s) 399
DrdI GACNNNNNNGTC 2 cut(s) 583, 2757
DseDI GACNNNNNNGTC 2 cut(s) 583, 2757
Eam1104I CTCTTC 4 cut(s) 360, 429, 512, 2991
EarI CTCTTC 4 cut(s) 360, 429, 512, 2991
EciI GGCGGA 2 cut(s) 698, 1269
Ecl136II GAGCTC 2 cut(s) 3475, 3776
Eco130I CCWWGG 2 cut(s) 2089, 2499
Eco147I AGGCCT 1 cut(s) 1056
Eco24I GRGCYC 3 cut(s) 287, 3477, 3778
Eco31I GGTCTC 1 cut(s) 569
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 4 cut(s) 70, 414, 914, 3328
Eco47III AGCGCT 1 cut(s) 973
Eco53kI GAGCTC 2 cut(s) 3475, 3776
Eco57I CTGAAG 2 cut(s) 690, 2880
Eco72I CACGTG 1 cut(s) 2935
Eco88I CYCGRG 1 cut(s) 286
Eco91I GGTNACC 1 cut(s) 2053
EcoICRI GAGCTC 2 cut(s) 3475, 3776
EcoO109I RGGNCCY 1 cut(s) 414
EcoO65I GGTNACC 1 cut(s) 2053
EcoRI GAATTC 1 cut(s) 1206
EcoRII CCWGG 5 cut(s) 201, 691, 2057, 2913, 3588
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 2 cut(s) 2089, 2499
EcoT22I ATGCAT 2 cut(s) 1332, 3259
EcoT38I GRGCYC 3 cut(s) 287, 3477, 3778
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 2089, 2499
Esp3I CGTCTC 1 cut(s) 501
FalI AAGNNNNNCTT 3 cut(s) 2613, 2645, 3788
FaqI GGGAC 4 cut(s) 83, 673, 752, 2344
FauNDI CATATG 1 cut(s) 3498
FbaI TGATCA 2 cut(s) 955, 1036
FblI GTMKAC 3 cut(s) 1443, 2505, 3555
FriOI GRGCYC 3 cut(s) 287, 3477, 3778
GlaI GCGC 8 cut(s) 33, 399, 799, 973, 1082, 1311, 2296, 2848
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 3 cut(s) 401, 801, 975
HaeIII GGCC 3 cut(s) 26, 1056, 1197
HapII CCGG 6 cut(s) 67, 73, 844, 1029, 1307, 3430
HhaI GCGC 8 cut(s) 34, 400, 800, 974, 1083, 1312, 2297, 2849
Hin1I GRCGYC 1 cut(s) 398
Hin6I GCGC 8 cut(s) 32, 398, 798, 972, 1081, 1310, 2295, 2847
HinP1I GCGC 8 cut(s) 32, 398, 798, 972, 1081, 1310, 2295, 2847
HincII GTYRAC 3 cut(s) 2506, 3178, 3639
HindII GTYRAC 3 cut(s) 2506, 3178, 3639
HpaI GTTAAC 1 cut(s) 3639
HpaII CCGG 6 cut(s) 67, 73, 844, 1029, 1307, 3430
HpyAV CCTTC 6 cut(s) 411, 1874, 2044, 3279, 3468, 3790
HpyCH4III ACNGT 9 cut(s) 657, 1174, 1441, 2200, 2420, 2683, 2765, 3360, 3571
HpyCH4IV ACGT 3 cut(s) 1015, 1240, 2934
HpyF3I CTNAG 8 cut(s) 609, 1179, 1220, 1334, 1359, 3449, 3525, 3530
HpySE526I ACGT 3 cut(s) 1015, 1240, 2934
Hsp92I GRCGYC 1 cut(s) 398
HspAI GCGC 8 cut(s) 32, 398, 798, 972, 1081, 1310, 2295, 2847
KasI GGCGCC 1 cut(s) 397
KpnI GGTACC 1 cut(s) 2620
Ksp22I TGATCA 2 cut(s) 955, 1036
KspAI GTTAAC 1 cut(s) 3639
MaeII ACGT 3 cut(s) 1015, 1240, 2934
MaeIII GTNAC 9 cut(s) 334, 802, 1011, 1130, 1435, 1945, 2053, 2759, 3296
MfeI CAATTG 2 cut(s) 345, 3652
MflI RGATCY 2 cut(s) 1316, 2020
MhlI GDGCHC 7 cut(s) 287, 1050, 1776, 3477, 3716, 3733, 3778
MluCI AATT 9 cut(s) 345, 507, 550, 847, 1206, 1343, 2551, 2638, 3652
Mly113I GGCGCC 1 cut(s) 398
MlyI GAGTC 5 cut(s) 695, 734, 779, 2468, 3677
MmeI TCCRAC 2 cut(s) 2138, 2402
Mph1103I ATGCAT 2 cut(s) 1332, 3259
MroXI GAANNNNTTC 4 cut(s) 1863, 2164, 2359, 3111
MseI TTAA 7 cut(s) 1497, 1629, 1761, 1881, 2631, 3638, 3814
MslI CAYNNNNRTG 3 cut(s) 158, 3135, 3563
MspA1I CMGCKG 1 cut(s) 3753
MspI CCGG 6 cut(s) 67, 73, 844, 1029, 1307, 3430
MspR9I CCNGG 7 cut(s) 68, 203, 693, 1030, 2059, 2915, 3590
MunI CAATTG 2 cut(s) 345, 3652
MvaI CCWGG 5 cut(s) 203, 693, 2059, 2915, 3590
MvnI CGCG 2 cut(s) 1081, 2229
NarI GGCGCC 1 cut(s) 398
NciI CCSGG 2 cut(s) 68, 1030
NdeI CATATG 1 cut(s) 3498
NlaIV GGNNCC 8 cut(s) 71, 178, 219, 399, 524, 915, 2618, 3209
NmeAIII GCCGAG 1 cut(s) 374
NmuCI GTSAC 6 cut(s) 334, 1130, 1435, 1945, 2053, 2759
NruI TCGCGA 1 cut(s) 2229
NsiI ATGCAT 2 cut(s) 1332, 3259
NspI RCATGY 5 cut(s) 296, 2760, 3047, 3562, 3712
PaeI GCATGC 1 cut(s) 296
PagI TCATGA 1 cut(s) 3733
PceI AGGCCT 1 cut(s) 1056
PciI ACATGT 3 cut(s) 2756, 3043, 3708
PdmI GAANNNNTTC 4 cut(s) 1863, 2164, 2359, 3111
PfoI TCCNGGA 1 cut(s) 2913
PinAI ACCGGT 1 cut(s) 72
PleI GAGTC 5 cut(s) 694, 734, 778, 2468, 3677
PluTI GGCGCC 1 cut(s) 401
PmaCI CACGTG 1 cut(s) 2935
PmlI CACGTG 1 cut(s) 2935
PpsI GAGTC 5 cut(s) 694, 734, 778, 2468, 3677
Ppu21I YACGTR 2 cut(s) 1016, 2935
PpuMI RGGWCCY 1 cut(s) 414
PscI ACATGT 3 cut(s) 2756, 3043, 3708
PsiI TTATAA 1 cut(s) 1070
Psp124BI GAGCTC 2 cut(s) 3477, 3778
Psp5II RGGWCCY 1 cut(s) 414
Psp6I CCWGG 5 cut(s) 201, 691, 2057, 2913, 3588
PspCI CACGTG 1 cut(s) 2935
PspEI GGTNACC 1 cut(s) 2053
PspGI CCWGG 5 cut(s) 201, 691, 2057, 2913, 3588
PspN4I GGNNCC 8 cut(s) 71, 178, 219, 399, 524, 915, 2618, 3209
PspPI GGNCC 4 cut(s) 70, 414, 914, 3328
PspPPI RGGWCCY 1 cut(s) 414
PstI CTGCAG 1 cut(s) 2398
PstNI CAGNNNCTG 2 cut(s) 593, 2858
PsuI RGATCY 2 cut(s) 1316, 2020
RruI TCGCGA 1 cut(s) 2229
RseI CAYNNNNRTG 3 cut(s) 158, 3135, 3563
SacI GAGCTC 2 cut(s) 3477, 3778
SalI GTCGAC 1 cut(s) 2504
SaqAI TTAA 7 cut(s) 1497, 1629, 1761, 1881, 2631, 3638, 3814
Sau96I GGNCC 4 cut(s) 70, 414, 914, 3328
ScaI AGTACT 4 cut(s) 1530, 1662, 1794, 1914
SchI GAGTC 5 cut(s) 695, 734, 779, 2468, 3677
ScrFI CCNGG 7 cut(s) 68, 203, 693, 1030, 2059, 2915, 3590
SduI GDGCHC 7 cut(s) 287, 1050, 1776, 3477, 3716, 3733, 3778
SfcI CTRYAG 3 cut(s) 1440, 2199, 2394
SfoI GGCGCC 1 cut(s) 399
SinI GGWCC 4 cut(s) 70, 414, 914, 3328
SmiMI CAYNNNNRTG 3 cut(s) 158, 3135, 3563
SmlI CTYRAG 4 cut(s) 125, 2314, 3487, 3722
SmoI CTYRAG 4 cut(s) 125, 2314, 3487, 3722
SpeI ACTAGT 2 cut(s) 1352, 1979
SphI GCATGC 1 cut(s) 296
Sse9I AATT 9 cut(s) 345, 507, 550, 847, 1206, 1343, 2551, 2638, 3652
SseBI AGGCCT 1 cut(s) 1056
SsiI CCGC 7 cut(s) 64, 683, 1079, 1165, 1280, 3658, 3753
SspDI GGCGCC 1 cut(s) 397
SstI GAGCTC 2 cut(s) 3477, 3778
StuI AGGCCT 1 cut(s) 1056
StyD4I CCNGG 7 cut(s) 66, 201, 691, 1028, 2057, 2913, 3588
StyI CCWWGG 2 cut(s) 2089, 2499
TaaI ACNGT 9 cut(s) 657, 1174, 1441, 2200, 2420, 2683, 2765, 3360, 3571
TaiI ACGT 3 cut(s) 1018, 1243, 2937
TasI AATT 9 cut(s) 345, 507, 550, 847, 1206, 1343, 2551, 2638, 3652
TatI WGTACW 7 cut(s) 634, 1528, 1660, 1792, 1912, 3046, 3693
TauI GCSGC 1 cut(s) 3660
Tru1I TTAA 7 cut(s) 1497, 1629, 1761, 1881, 2631, 3638, 3814
Tru9I TTAA 7 cut(s) 1497, 1629, 1761, 1881, 2631, 3638, 3814
TseFI GTSAC 6 cut(s) 334, 1130, 1435, 1945, 2053, 2759
Tsp45I GTSAC 6 cut(s) 334, 1130, 1435, 1945, 2053, 2759
TspGWI ACGGA 3 cut(s) 993, 2556, 2599
VneI GTGCAC 1 cut(s) 3712
VpaK11BI GGWCC 4 cut(s) 70, 414, 914, 3328
XapI RAATTY 4 cut(s) 507, 847, 1206, 1343
XceI RCATGY 5 cut(s) 296, 2760, 3047, 3562, 3712
XcmI CCANNNNNNNNNTGG 2 cut(s) 481, 2589
XmiI GTMKAC 3 cut(s) 1443, 2505, 3555
XmnI GAANNNNTTC 4 cut(s) 1863, 2164, 2359, 3111
ZrmI AGTACT 4 cut(s) 1530, 1662, 1794, 1914
Zsp2I ATGCAT 2 cut(s) 1332, 3259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.