Rmu_ssc0000249.1_g000008

Serine arginine repetitive matrix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000249.1
OG0007711
Physical Location & Seq
Forward (+)
33503 .. 39997
6495 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2694 bp
atggcaaaaatccccagcggggcggagctccattggatacccgcccctaatggggggagaattcggggacaaatggggaatggggatggggatccccaatccccgctatctaagattggggatgaggtggtgatgggtacttcggctgatcaggacacgaggttttccaataagcaagccaagctgctgaaatcgcagaaatttgcacctgaattggaacatttggtggacatgaggaaggtgaatatggatgtgctccgtccatggattgctaatagggtaaccgagcttcttgggttcgaagatgaagttcttatcaacttcatttatggtcttttggatgtgaaggaagtgaatggaaaggagattcaaatttcactgactggatttatggagaaaaatacggtgaagtttatgagagagctttggacgcttcttctcagtgcgcaaaacaatgccagtggtgtccctcaacagttcttggatgccaaacaagaggaagcacgaaaaaagaaggaggaggcagatgcaattgaaactcagaggaagaaagataaggagacgcttgaactagagagatcgaagacgatggaaggaggggttgataagaaatcaagcaatgctgctttggagccaaactcaaatcgtgtgtcaccaagggcttcaagtgatcgtcctgatgatgaaagagggaatggcaagaggaatggtgtgaagccaagtaacaaggtttctagatctccagattcagcagattcagattcatctttgtcccctcggtttggtgaacgagacaggtcaagaagtgtatccgtttcaccaaaagcacgaggacggtccatttcttctgaaaggggaaggggttatcgctctccacaaagacgaccgattacgcctaggaggcgttcacctgagggatcactctccccaaaaggaagatcaccttattccaggcgaggatcaagatcaaaatcaccacgcagatcgcgctctcctatagtgcgtcgagctcgttctccgtatcatcgtaggtcacgatctcctggacgccgcaggtcacctcctcctttccgaagccgcaggtcaccctcaccttttcgaggccgtagatcaccctcaccttttcgaggccgtagatcaccctatcggcgccgaagatcaccctcacctccccgccgccgcagattaccctcacctattcgtaatcgcagatcaccatcacctatccggcgtcgcagatcaccctcccctctgcggcgacggacatcacctcttgtacggcgtcgcaggtccccctcacccgcacgtcgcagatcaccctctcctgttcgatcatcatatcgaagatctccacgtaacaggtatgcatctccagtgcaacggaggtcaccggttcgtggtggcagaaggtccccacctcttcgacgtaggtctccttcgccttctgatcgaagttcttcatctcctattcggcgtgattccccttcaccaatcaggagaacaacaaagcagcagagatctcccctgcaatcccaccaagaaagaacaaggtccattgagaacctgtctccagcgccacaacaatcttctggttctgaggggtcactgcagagggaatcgaagaatggaaatgattaccgcaaaagagttgcagctttgtcaccatcaccagagaagtctcctccaatgtcagaatctccaccaggtgcaaggagtgtgagggaaaagaggagattgtccagtccatatgaaagtcctgtgaaacgaccaagggagcaaattgctcgttatggtagctcaagtccttcaaggaaaccaagagaacaaaaacttcgtcgtgacagtcccaaagaaagtgaagaagaggaggatgcgggtaacagacccaagtccttagagagaagatcaagagacacatcacggatcagtaagcagataatttctcctgctaagattcctaataaggaagagtactctcctgaaagagtagcatctggtcaaaggtttactgaatctaaaagctatttggataatactgaaagaagaaacaaagagcaggaaatgaagagtggaaaatcctctggcagggggtcaaatgtaccaattgtctataaagactatctgccaggtgagacacaacgggcatcaaatcctggtgaaggtaaaaaagctgatgataagaaccgctctcaattaaagaataccaaggacagcgaacatcgtaaacctggatctttacgctcatctgttgaaatgattgatcatagtaatcagagtggcggtttggattctggttctgaggacagtgacaaaggcagaagcgaagacaaggaaaagagaaagaataagagatcagagaggcaagaagtgacttcagatgatgattacagttatgattctgcaattgaggacagaaaggaggctaaaaggaggaggaaagaggaaaagaggttgcggaaggagaagaagcgccgaagacgtgaggagcgacgacgcagaagggaagaacgtcgtgcagagaagcagaaagtgaagaactccagtgatgcttctggttcagatggtgaacatgtcaccggaagggggtttcattcaagtgacaatgaagaagaggctgttaagaagaaacttgagattgaattgcgcaagaaggctctcgaatcactcaaagcaaaaaagggcatcagtcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
PWI

Protein Analysis

897

Amino Acids

102.51

Weight (kDa)

11.23

Isoelectric Point (pI)

120.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWI PF01480 84 - 153 6.1e-27 PWI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 447, 2645
Acc36I ACCTGC 3 cut(s) 1044, 1071, 1278
AccB1I GGYRCC 1 cut(s) 1149
AccBSI CCGCTC 1 cut(s) 2187
AccII CGCG 1 cut(s) 988
AclWI GGATC 6 cut(s) 86, 99, 925, 967, 1931, 2239
AcsI RAATTY 3 cut(s) 60, 200, 372
AcuI CTGAAG 1 cut(s) 2358
AcyI GRCGYC 4 cut(s) 1048, 1150, 1231, 1282
AdeI CACNNNGTG 1 cut(s) 1706
AfaI GTAC 4 cut(s) 139, 1278, 1973, 2100
AflIII ACRYGT 1 cut(s) 2569
AgeI ACCGGT 1 cut(s) 1390
AgsI TTSAA 8 cut(s) 371, 536, 569, 666, 1809, 2252, 2595, 2639
AjiI CACGTC 2 cut(s) 1307, 2480
AjnI CCWGG 6 cut(s) 950, 1042, 1702, 2125, 2152, 2227
AluBI AGCT 9 cut(s) 28, 184, 289, 424, 1010, 1655, 1797, 2022, 2171
AluI AGCT 9 cut(s) 28, 184, 289, 424, 1010, 1655, 1797, 2022, 2171
Alw21I GWGCWC 3 cut(s) 30, 258, 1012
Alw26I GTCTC 7 cut(s) 554, 786, 1437, 1572, 1683, 1905, 2126
AlwI GGATC 6 cut(s) 86, 99, 925, 967, 1931, 2239
AoxI GGCC 2 cut(s) 1102, 1129
ApeKI GCWGC 4 cut(s) 184, 623, 1510, 1652
ApoI RAATTY 3 cut(s) 60, 200, 372
ArsI GACNNNNNNTTYG 4 cut(s) 635, 667, 1819, 1851
AsiGI ACCGGT 1 cut(s) 1390
Asp700I GAANNNNTTC 1 cut(s) 1456
AspA2I CCTAGG 1 cut(s) 896
AspLEI GCGC 6 cut(s) 448, 990, 1152, 1576, 2472, 2646
AspS9I GGNCC 4 cut(s) 837, 1290, 1410, 1551
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 4 cut(s) 837, 1290, 1410, 1551
AvrII CCTAGG 1 cut(s) 896
AxyI CCTNAGG 1 cut(s) 912
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 1149
BanII GRGCYC 2 cut(s) 30, 1012
BarI GAAGNNNNNNTAC 4 cut(s) 273, 305, 1420, 1452
BauI CACGAG 2 cut(s) 157, 828
BbsI GAAGAC 3 cut(s) 590, 2331, 2482
Bbv12I GWGCWC 3 cut(s) 30, 258, 1012
BbvI GCAGC 4 cut(s) 171, 610, 1522, 1664
BccI CCATC 6 cut(s) 80, 127, 583, 1225, 1672, 2555
BceAI ACGGC 3 cut(s) 1089, 1116, 1295
BcgI CGANNNNNNTGC 2 cut(s) 1766, 1800
BciT130I CCWGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
BciVI GTATCC 2 cut(s) 30, 820
BclI TGATCA 2 cut(s) 148, 2260
BcoDI GTCTC 7 cut(s) 554, 786, 1437, 1572, 1683, 1905, 2126
BfaI CTAG 3 cut(s) 572, 735, 897
BfmI CTRYAG 2 cut(s) 996, 1607
BfoI RGCGCY 3 cut(s) 1153, 1577, 2473
BfuAI ACCTGC 3 cut(s) 1044, 1071, 1278
BfuI GTATCC 2 cut(s) 30, 820
BglI GCCNNNNNGGC 1 cut(s) 901
BglII AGATCT 3 cut(s) 737, 1346, 1517
BisI GCNGC 9 cut(s) 185, 624, 1051, 1078, 1177, 1180, 1256, 1511, 1653
BlnI CCTAGG 1 cut(s) 896
BlsI GCNGC 9 cut(s) 186, 625, 1052, 1079, 1178, 1181, 1257, 1512, 1654
BmcAI AGTACT 1 cut(s) 1973
Bme1390I CCNGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
Bme18I GGWCC 4 cut(s) 837, 1290, 1410, 1551
BmgBI CACGTC 2 cut(s) 1307, 2480
BmgT120I GGNCC 4 cut(s) 837, 1290, 1410, 1551
BmiI GGNNCC 5 cut(s) 93, 633, 1151, 1292, 1412
BmrFI CCNGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
BmsI GCATC 7 cut(s) 475, 517, 1376, 1861, 2000, 2153, 2536
BoxI GACNNNNGTC 2 cut(s) 1429, 1888
BpiI GAAGAC 3 cut(s) 590, 2331, 2482
BplI GAGNNNNNCTC 2 cut(s) 623, 655
BpmI CTGGAG 4 cut(s) 726, 1356, 1554, 2524
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 1783, 2651
BsaAI YACGTR 1 cut(s) 1355
BsaBI GATNNNNATC 4 cut(s) 90, 964, 970, 1216
BsaHI GRCGYC 4 cut(s) 1048, 1150, 1231, 1282
BsaI GGTCTC 1 cut(s) 1437
BsaJI CCNNGG 6 cut(s) 263, 656, 776, 896, 1769, 2205
BsaWI WCCGGW 2 cut(s) 1390, 2576
Bse118I RCCGGY 1 cut(s) 1390
Bse1I ACTGG 5 cut(s) 388, 459, 1373, 1740, 2541
Bse21I CCTNAGG 1 cut(s) 912
Bse3DI GCAATG 1 cut(s) 625
Bse8I GATNNNNATC 4 cut(s) 90, 964, 970, 1216
BseBI CCWGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
BseDI CCNNGG 6 cut(s) 263, 656, 776, 896, 1769, 2205
BseGI GGATG 6 cut(s) 91, 127, 256, 346, 490, 1876
BseJI GATNNNNATC 4 cut(s) 90, 964, 970, 1216
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 5 cut(s) 454, 554, 903, 1587, 2289
BseNI ACTGG 5 cut(s) 388, 459, 1373, 1740, 2541
BseRI GAGGAG 7 cut(s) 533, 1053, 1671, 1744, 1880, 2446, 2498
BseXI GCAGC 4 cut(s) 171, 610, 1522, 1664
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 2535
Bsh1236I CGCG 1 cut(s) 988
Bsh1285I CGRYCG 1 cut(s) 887
BshFI GGCC 2 cut(s) 1104, 1131
BshNI GGYRCC 1 cut(s) 1149
BshTI ACCGGT 1 cut(s) 1390
BsiEI CGRYCG 1 cut(s) 887
BsiHKAI GWGCWC 3 cut(s) 30, 258, 1012
BsiSI CCGG 3 cut(s) 1228, 1391, 2577
BslFI GGGAC 6 cut(s) 81, 452, 757, 1276, 1396, 1830
BsmAI GTCTC 7 cut(s) 554, 786, 1437, 1572, 1683, 1905, 2126
BsmBI CGTCTC 1 cut(s) 554
BsmFI GGGAC 6 cut(s) 81, 452, 757, 1276, 1396, 1830
BsnI GGCC 2 cut(s) 1104, 1131
Bso31I GGTCTC 1 cut(s) 1437
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 3 cut(s) 30, 258, 1012
Bsp19I CCATGG 1 cut(s) 263
BspANI GGCC 2 cut(s) 1104, 1131
BspCNI CTCAG 5 cut(s) 453, 553, 904, 1588, 2290
BspFNI CGCG 1 cut(s) 988
BspLI GGNNCC 5 cut(s) 93, 633, 1151, 1292, 1412
BspMAI CTGCAG 1 cut(s) 1611
BspMI ACCTGC 3 cut(s) 1044, 1071, 1278
BspPI GGATC 6 cut(s) 86, 99, 925, 967, 1931, 2239
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 1149
BspTNI GGTCTC 1 cut(s) 1437
BsrBI CCGCTC 1 cut(s) 2187
BsrDI GCAATG 1 cut(s) 625
BsrFI RCCGGY 1 cut(s) 1390
BsrI ACTGG 5 cut(s) 388, 459, 1373, 1740, 2541
BssAI RCCGGY 1 cut(s) 1390
BssECI CCNNGG 6 cut(s) 263, 656, 776, 896, 1769, 2205
BssNI GRCGYC 4 cut(s) 1048, 1150, 1231, 1282
BssSI CACGAG 2 cut(s) 157, 828
BssT1I CCWWGG 5 cut(s) 263, 656, 896, 1769, 2205
Bst2BI CACGAG 2 cut(s) 157, 828
Bst2UI CCWGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
Bst4CI ACNGT 6 cut(s) 406, 477, 837, 1844, 2306, 2390
Bst6I CTCTTC 5 cut(s) 1425, 1857, 1962, 2060, 2604
BstACI GRCGYC 4 cut(s) 1048, 1150, 1231, 1282
BstBAI YACGTR 1 cut(s) 1355
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 8 cut(s) 111, 440, 540, 912, 1596, 1894, 1950, 2298
BstDSI CCRYGG 1 cut(s) 263
BstEII GGTNACC 4 cut(s) 280, 1056, 1083, 1386
BstENI CCTNNNNNAGG 4 cut(s) 1098, 1125, 2083, 2157
BstF5I GGATG 6 cut(s) 91, 127, 256, 346, 490, 1876
BstFNI CGCG 1 cut(s) 988
BstH2I RGCGCY 3 cut(s) 1153, 1577, 2473
BstHHI GCGC 6 cut(s) 448, 990, 1152, 1576, 2472, 2646
BstMAI GTCTC 7 cut(s) 554, 786, 1437, 1572, 1683, 1905, 2126
BstMCI CGRYCG 1 cut(s) 887
BstMWI GCNNNNNNNGC 5 cut(s) 181, 193, 430, 901, 987
BstNI CCWGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
BstNSI RCATGY 1 cut(s) 2573
BstPAI GACNNNNGTC 2 cut(s) 1429, 1888
BstPI GGTNACC 4 cut(s) 280, 1056, 1083, 1386
BstSCI CCNGG 6 cut(s) 950, 1042, 1702, 2125, 2152, 2227
BstSFI CTRYAG 2 cut(s) 996, 1607
BstUI CGCG 1 cut(s) 988
BstV1I GCAGC 4 cut(s) 171, 610, 1522, 1664
BstV2I GAAGAC 3 cut(s) 590, 2331, 2482
BstX2I RGATCY 5 cut(s) 91, 737, 1346, 1517, 2231
BstYI RGATCY 5 cut(s) 91, 737, 1346, 1517, 2231
Bsu36I CCTNAGG 1 cut(s) 912
BsuI GTATCC 2 cut(s) 30, 820
BsuRI GGCC 2 cut(s) 1104, 1131
BtgI CCRYGG 1 cut(s) 263
BtrI CACGTC 2 cut(s) 1307, 2480
BtsCI GGATG 6 cut(s) 91, 127, 256, 346, 490, 1876
BtsI GCAGTG 1 cut(s) 1604
BtsIMutI CAGTG 7 cut(s) 377, 448, 466, 1380, 1604, 2311, 2548
BveI ACCTGC 3 cut(s) 1044, 1071, 1278
Cac8I GCNNGC 1 cut(s) 177
CfoI GCGC 6 cut(s) 448, 990, 1152, 1576, 2472, 2646
Cfr10I RCCGGY 1 cut(s) 1390
Cfr13I GGNCC 4 cut(s) 837, 1290, 1410, 1551
CseI GACGC 7 cut(s) 439, 571, 992, 1056, 1220, 1271, 2502
CsiI ACCWGGT 1 cut(s) 1702
Csp6I GTAC 4 cut(s) 138, 1277, 1972, 2099
CspAI ACCGGT 1 cut(s) 1390
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 3 cut(s) 232, 264, 2570
CviQI GTAC 4 cut(s) 138, 1277, 1972, 2099
DdeI CTNAG 8 cut(s) 111, 440, 540, 912, 1596, 1894, 1950, 2298
DinI GGCGCC 1 cut(s) 1151
DraIII CACNNNGTG 1 cut(s) 1706
Eam1104I CTCTTC 5 cut(s) 1425, 1857, 1962, 2060, 2604
EarI CTCTTC 5 cut(s) 1425, 1857, 1962, 2060, 2604
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 2 cut(s) 28, 1010
Eco130I CCWWGG 5 cut(s) 263, 656, 896, 1769, 2205
Eco24I GRGCYC 2 cut(s) 30, 1012
Eco31I GGTCTC 1 cut(s) 1437
Eco47I GGWCC 4 cut(s) 837, 1290, 1410, 1551
Eco53kI GAGCTC 2 cut(s) 28, 1010
Eco57I CTGAAG 1 cut(s) 2358
Eco81I CCTNAGG 1 cut(s) 912
Eco91I GGTNACC 4 cut(s) 280, 1056, 1083, 1386
EcoICRI GAGCTC 2 cut(s) 28, 1010
EcoNI CCTNNNNNAGG 4 cut(s) 1098, 1125, 2083, 2157
EcoO109I RGGNCCY 2 cut(s) 1290, 1410
EcoO65I GGTNACC 4 cut(s) 280, 1056, 1083, 1386
EcoRI GAATTC 1 cut(s) 60
EcoRII CCWGG 6 cut(s) 950, 1042, 1702, 2125, 2152, 2227
EcoT14I CCWWGG 5 cut(s) 263, 656, 896, 1769, 2205
EcoT22I ATGCAT 1 cut(s) 1369
EcoT38I GRGCYC 2 cut(s) 30, 1012
EgeI GGCGCC 1 cut(s) 1151
EheI GGCGCC 1 cut(s) 1151
ErhI CCWWGG 5 cut(s) 263, 656, 896, 1769, 2205
Esp3I CGTCTC 1 cut(s) 554
FaeI CATG 3 cut(s) 235, 267, 2573
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 6 cut(s) 81, 452, 757, 1276, 1396, 1830
FatI CATG 3 cut(s) 231, 263, 2569
FauI CCCGC 6 cut(s) 11, 49, 111, 1181, 1309, 1867
FauNDI CATATG 1 cut(s) 1747
FbaI TGATCA 2 cut(s) 148, 2260
Fnu4HI GCNGC 9 cut(s) 185, 624, 1051, 1078, 1177, 1180, 1256, 1511, 1653
FokI GGATG 6 cut(s) 98, 134, 263, 353, 497, 1883
FriOI GRGCYC 2 cut(s) 30, 1012
Fsp4HI GCNGC 9 cut(s) 185, 624, 1051, 1078, 1177, 1180, 1256, 1511, 1653
FspBI CTAG 3 cut(s) 572, 735, 897
FspI TGCGCA 2 cut(s) 447, 2645
GlaI GCGC 6 cut(s) 447, 989, 1151, 1575, 2471, 2645
GluI GCNGC 9 cut(s) 185, 624, 1051, 1078, 1177, 1180, 1256, 1511, 1653
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 4 cut(s) 726, 1356, 1554, 2524
HaeII RGCGCY 3 cut(s) 1153, 1577, 2473
HaeIII GGCC 2 cut(s) 1104, 1131
HapII CCGG 3 cut(s) 1228, 1391, 2577
HgaI GACGC 7 cut(s) 439, 571, 992, 1056, 1220, 1271, 2502
HhaI GCGC 6 cut(s) 448, 990, 1152, 1576, 2472, 2646
Hin1I GRCGYC 4 cut(s) 1048, 1150, 1231, 1282
Hin1II CATG 3 cut(s) 235, 267, 2573
Hin6I GCGC 6 cut(s) 446, 988, 1150, 1574, 2470, 2644
HinP1I GCGC 6 cut(s) 446, 988, 1150, 1574, 2470, 2644
HpaII CCGG 3 cut(s) 1228, 1391, 2577
Hpy166II GTNNAC 6 cut(s) 229, 788, 908, 2007, 2225, 2567
Hpy8I GTNNAC 6 cut(s) 229, 788, 908, 2007, 2225, 2567
HpyCH4III ACNGT 6 cut(s) 406, 477, 837, 1844, 2306, 2390
HpyCH4IV ACGT 5 cut(s) 1306, 1354, 1426, 2479, 2509
HpyF10VI GCNNNNNNNGC 5 cut(s) 181, 193, 430, 901, 987
HpyF3I CTNAG 8 cut(s) 111, 440, 540, 912, 1596, 1894, 1950, 2298
HpySE526I ACGT 5 cut(s) 1306, 1354, 1426, 2479, 2509
Hsp92I GRCGYC 4 cut(s) 1048, 1150, 1231, 1282
Hsp92II CATG 3 cut(s) 235, 267, 2573
HspAI GCGC 6 cut(s) 446, 988, 1150, 1574, 2470, 2644
KasI GGCGCC 1 cut(s) 1149
Ksp22I TGATCA 2 cut(s) 148, 2260
LmnI GCTCC 6 cut(s) 25, 33, 261, 631, 1774, 2485
Lsp1109I GCAGC 4 cut(s) 171, 610, 1522, 1664
LweI GCATC 7 cut(s) 475, 517, 1376, 1861, 2000, 2153, 2536
MabI ACCWGGT 1 cut(s) 1702
MaeI CTAG 3 cut(s) 572, 735, 897
MaeII ACGT 5 cut(s) 1306, 1354, 1426, 2479, 2509
MbiI CCGCTC 1 cut(s) 2187
MfeI CAATTG 3 cut(s) 531, 2103, 2403
MflI RGATCY 5 cut(s) 91, 737, 1346, 1517, 2231
MhlI GDGCHC 3 cut(s) 30, 258, 1012
Mly113I GGCGCC 1 cut(s) 1150
Mph1103I ATGCAT 1 cut(s) 1369
MroXI GAANNNNTTC 1 cut(s) 1456
MseI TTAA 2 cut(s) 2195, 2619
MslI CAYNNNNRTG 1 cut(s) 2595
MspA1I CMGCKG 1 cut(s) 18
MspI CCGG 3 cut(s) 1228, 1391, 2577
MspR9I CCNGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
MunI CAATTG 3 cut(s) 531, 2103, 2403
MvaI CCWGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
MvnI CGCG 1 cut(s) 988
MwoI GCNNNNNNNGC 5 cut(s) 181, 193, 430, 901, 987
NarI GGCGCC 1 cut(s) 1150
NcoI CCATGG 1 cut(s) 263
NdeI CATATG 1 cut(s) 1747
NlaIII CATG 3 cut(s) 235, 267, 2573
NlaIV GGNNCC 5 cut(s) 93, 633, 1151, 1292, 1412
NsbI TGCGCA 2 cut(s) 447, 2645
NsiI ATGCAT 1 cut(s) 1369
NspI RCATGY 1 cut(s) 2573
NspV TTCGAA 1 cut(s) 300
PciI ACATGT 1 cut(s) 2569
PcsI WCGNNNNNNNCGW 2 cut(s) 1033, 2485
PdmI GAANNNNTTC 1 cut(s) 1456
PfoI TCCNGGA 1 cut(s) 1042
PinAI ACCGGT 1 cut(s) 1390
PkrI GCNGC 9 cut(s) 186, 625, 1052, 1079, 1178, 1181, 1257, 1512, 1654
PluTI GGCGCC 1 cut(s) 1153
Ppu21I YACGTR 1 cut(s) 1355
PpuMI RGGWCCY 2 cut(s) 1290, 1410
PscI ACATGT 1 cut(s) 2569
PshAI GACNNNNGTC 2 cut(s) 1429, 1888
Psp124BI GAGCTC 2 cut(s) 30, 1012
Psp5II RGGWCCY 2 cut(s) 1290, 1410
Psp6I CCWGG 6 cut(s) 950, 1042, 1702, 2125, 2152, 2227
PspEI GGTNACC 4 cut(s) 280, 1056, 1083, 1386
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 6 cut(s) 950, 1042, 1702, 2125, 2152, 2227
PspN4I GGNNCC 5 cut(s) 93, 633, 1151, 1292, 1412
PspPI GGNCC 4 cut(s) 837, 1290, 1410, 1551
PspPPI RGGWCCY 2 cut(s) 1290, 1410
PstI CTGCAG 1 cut(s) 1611
PsuI RGATCY 5 cut(s) 91, 737, 1346, 1517, 2231
RsaI GTAC 4 cut(s) 139, 1278, 1973, 2100
RsaNI GTAC 4 cut(s) 138, 1277, 1972, 2099
RseI CAYNNNNRTG 1 cut(s) 2595
SacI GAGCTC 2 cut(s) 30, 1012
SaqAI TTAA 2 cut(s) 2195, 2619
SatI GCNGC 9 cut(s) 185, 624, 1051, 1078, 1177, 1180, 1256, 1511, 1653
Sau96I GGNCC 4 cut(s) 837, 1290, 1410, 1551
ScaI AGTACT 1 cut(s) 1973
ScrFI CCNGG 6 cut(s) 952, 1044, 1704, 2127, 2154, 2229
SduI GDGCHC 3 cut(s) 30, 258, 1012
SexAI ACCWGGT 1 cut(s) 1702
SfaNI GCATC 7 cut(s) 475, 517, 1376, 1861, 2000, 2153, 2536
SfcI CTRYAG 2 cut(s) 996, 1607
SfoI GGCGCC 1 cut(s) 1151
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 4 cut(s) 837, 1290, 1410, 1551
SmiMI CAYNNNNRTG 1 cut(s) 2595
SmlI CTYRAG 2 cut(s) 1798, 2630
SmoI CTYRAG 2 cut(s) 1798, 2630
SspDI GGCGCC 1 cut(s) 1149
SspMI CTAG 3 cut(s) 572, 735, 897
SstI GAGCTC 2 cut(s) 30, 1012
StyD4I CCNGG 6 cut(s) 950, 1042, 1702, 2125, 2152, 2227
StyI CCWWGG 5 cut(s) 263, 656, 896, 1769, 2205
TaaI ACNGT 6 cut(s) 406, 477, 837, 1844, 2306, 2390
TaiI ACGT 5 cut(s) 1309, 1357, 1429, 2482, 2512
TaqII GACCGA 1 cut(s) 901
TatI WGTACW 1 cut(s) 1971
TauI GCSGC 5 cut(s) 1053, 1080, 1179, 1182, 1258
Tru1I TTAA 2 cut(s) 2195, 2619
Tru9I TTAA 2 cut(s) 2195, 2619
TscAI CASTG 7 cut(s) 384, 448, 466, 1380, 1611, 2311, 2548
TseI GCWGC 4 cut(s) 184, 623, 1510, 1652
TspDTI ATGAA 9 cut(s) 313, 321, 699, 753, 1449, 1764, 2078, 2579, 2619
TspGWI ACGGA 6 cut(s) 248, 802, 1008, 1276, 1396, 1936
TspRI CASTG 7 cut(s) 384, 448, 466, 1380, 1611, 2311, 2548
VpaK11BI GGWCC 4 cut(s) 837, 1290, 1410, 1551
XagI CCTNNNNNAGG 4 cut(s) 1098, 1125, 2083, 2157
XapI RAATTY 3 cut(s) 60, 200, 372
XbaI TCTAGA 1 cut(s) 734
XceI RCATGY 1 cut(s) 2573
XmaJI CCTAGG 1 cut(s) 896
XmnI GAANNNNTTC 1 cut(s) 1456
XspI CTAG 3 cut(s) 572, 735, 897
ZrmI AGTACT 1 cut(s) 1973
Zsp2I ATGCAT 1 cut(s) 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.