Rmu_ssc0000386.1_g000046

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000386.1
OG0011606
Physical Location & Seq
Reverse (-)
250823 .. 251665
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000386.1_g000046.1.cds

Sequence Viewer

Length: 843 bp
atgggttgtgcagtttggctcatagatcagctaagaaaggcaagttttacaggggaacaaaatatctgcagggtgttcagaggaaataactatactttctgggtggagaactacagaaataaaaatggatccttcatgaaactttctcgatgtggaagatacaaagctgtccattccatcattttaccgaaaggggaaggaaatgaaggctggcaacggatggaggagaatctaaacactttcatgtttgggaaaagcaaggaggaagtgaagcaagtccaagagaaaatgatgattcaaaaacaaagggagcggtttccagaaaagcagctggaaagcaatcgtagcttcatgaaacaaggaggtactaagacgcagaagctggcggataccaaaaaatcttgggcttgtacagtgatttgtcaaaggcaggctgtttttcaatcttggaagaacattgaaagaggcttatcaaagtggctgggcaaagagatagagctttatccttttcaaaacaacagagcttttttcgtctgcgaaaactgtgaggaggcagaacagattgctagtcatggaaaactcccattgctggggcaaccagaggtccttctttttgagtgggcacaaggattgacaaacaatctgaagaaggtggtgagctacggaggatggatagcagtgaagggactcccacttcattggtggtcgcagaatattttaagcagatcggcgatacttgtggtggcttgcttgaagtggataacaggacagaaggcttcaaacgtctgttcgaagctcggatcagagtcagagagaacgagattggattcctgcctgaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

32.32

Weight (kDa)

9.49

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 313
AciI CCGC 2 cut(s) 313, 386
AclWI GGATC 3 cut(s) 123, 136, 808
AcuI CTGAAG 1 cut(s) 665
AfaI GTAC 2 cut(s) 367, 412
AfiI CCNNNNNNNGG 2 cut(s) 589, 590
AgsI TTSAA 6 cut(s) 299, 443, 461, 512, 754, 780
AluBI AGCT 9 cut(s) 31, 167, 331, 348, 382, 499, 524, 660, 796
AluI AGCT 9 cut(s) 31, 167, 331, 348, 382, 499, 524, 660, 796
AlwI GGATC 3 cut(s) 123, 136, 808
AlwNI CAGNNNCTG 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 604
AsuHPI GGTGA 1 cut(s) 667
AsuII TTCGAA 1 cut(s) 791
AvaII GGWCC 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 625
BamHI GGATCC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 340
BccI CCATC 3 cut(s) 185, 214, 663
BciVI GTATCC 1 cut(s) 382
BfaI CTAG 2 cut(s) 567, 841
BfmI CTRYAG 2 cut(s) 67, 112
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 1 cut(s) 329
BlsI GCNGC 1 cut(s) 330
Bme18I GGWCC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 1 cut(s) 130
Bpu14I TTCGAA 1 cut(s) 791
Bsc4I CCNNNNNNNGG 2 cut(s) 589, 590
Bse3DI GCAATG 1 cut(s) 584
BseGI GGATG 2 cut(s) 225, 674
BseLI CCNNNNNNNGG 2 cut(s) 589, 590
BseMI GCAATG 1 cut(s) 584
BseRI GAGGAG 2 cut(s) 239, 563
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 1 cut(s) 340
BseYI CCCAGC 2 cut(s) 481, 589
BsgI GTGCAG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 699
BslI CCNNNNNNNGG 2 cut(s) 589, 590
BsmFI GGGAC 1 cut(s) 699
Bsp119I TTCGAA 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 625
Bsp1407I TGTACA 1 cut(s) 410
Bsp143I GATC 4 cut(s) 25, 128, 725, 800
BspACI CCGC 2 cut(s) 313, 386
BspHI TCATGA 2 cut(s) 135, 351
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 1 cut(s) 71
BspPI GGATC 3 cut(s) 123, 136, 808
BspT104I TTCGAA 1 cut(s) 791
BsrBI CCGCTC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 584
BsrGI TGTACA 1 cut(s) 410
BssMI GATC 4 cut(s) 25, 128, 725, 800
Bst4CI ACNGT 2 cut(s) 415, 545
BstAUI TGTACA 1 cut(s) 410
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 4 cut(s) 212, 384, 432, 748
BstDEI CTNAG 2 cut(s) 32, 369
BstF5I GGATG 2 cut(s) 225, 674
BstKTI GATC 4 cut(s) 28, 131, 728, 803
BstMBI GATC 4 cut(s) 25, 128, 725, 800
BstMWI GCNNNNNNNGC 1 cut(s) 345
BstSFI CTRYAG 2 cut(s) 67, 112
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 128
BstXI CCANNNNNNTGG 1 cut(s) 699
BstYI RGATCY 1 cut(s) 128
BsuI GTATCC 1 cut(s) 382
BtsCI GGATG 2 cut(s) 225, 674
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 2 cut(s) 420, 684
Cac8I GCNNGC 4 cut(s) 212, 384, 432, 748
CaiI CAGNNNCTG 1 cut(s) 382
CciI TCATGA 2 cut(s) 135, 351
Cfr13I GGNCC 1 cut(s) 604
CseI GACGC 1 cut(s) 382
Csp6I GTAC 2 cut(s) 366, 411
CviAII CATG 4 cut(s) 136, 244, 352, 572
CviQI GTAC 2 cut(s) 366, 411
DdeI CTNAG 2 cut(s) 32, 369
DpnI GATC 4 cut(s) 27, 130, 727, 802
DpnII GATC 4 cut(s) 25, 128, 725, 800
EciI GGCGGA 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 665
EcoO109I RGGNCCY 1 cut(s) 604
FaeI CATG 4 cut(s) 139, 247, 355, 575
FaiI YATR 6 cut(s) 23, 93, 137, 245, 353, 573
FaqI GGGAC 1 cut(s) 699
FatI CATG 4 cut(s) 135, 243, 351, 571
Fnu4HI GCNGC 1 cut(s) 329
FokI GGATG 2 cut(s) 232, 681
Fsp4HI GCNGC 1 cut(s) 329
FspBI CTAG 2 cut(s) 567, 841
GluI GCNGC 1 cut(s) 329
GsaI CCCAGC 2 cut(s) 485, 593
HgaI GACGC 1 cut(s) 382
Hin1II CATG 4 cut(s) 139, 247, 355, 575
HinfI GANTC 5 cut(s) 229, 295, 687, 806, 827
HphI GGTGA 1 cut(s) 667
Hpy188I TCNGA 5 cut(s) 80, 645, 800, 805, 811
Hpy188III TCNNGA 4 cut(s) 136, 147, 320, 352
HpyAV CCTTC 7 cut(s) 142, 191, 200, 617, 643, 676, 766
HpyCH4III ACNGT 2 cut(s) 415, 545
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 2 cut(s) 11, 69
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
HpyF3I CTNAG 2 cut(s) 32, 369
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 4 cut(s) 139, 247, 355, 575
Kzo9I GATC 4 cut(s) 25, 128, 725, 800
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 340
MaeI CTAG 2 cut(s) 567, 841
MaeII ACGT 1 cut(s) 783
MalI GATC 4 cut(s) 27, 130, 727, 802
MbiI CCGCTC 1 cut(s) 313
MboI GATC 4 cut(s) 25, 128, 725, 800
MboII GAAGA 3 cut(s) 168, 463, 658
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 625
MlyI GAGTC 2 cut(s) 681, 815
MnlI CCTC 9 cut(s) 74, 217, 256, 356, 458, 541, 544, 595, 659
MseI TTAA 1 cut(s) 719
MslI CAYNNNNRTG 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 331
MwoI GCNNNNNNNGC 1 cut(s) 345
NdeII GATC 4 cut(s) 25, 128, 725, 800
NlaIII CATG 4 cut(s) 139, 247, 355, 575
NlaIV GGNNCC 1 cut(s) 130
NspV TTCGAA 1 cut(s) 791
PagI TCATGA 2 cut(s) 135, 351
PfeI GAWTC 3 cut(s) 229, 295, 827
PkrI GCNGC 1 cut(s) 330
PleI GAGTC 2 cut(s) 681, 814
PpsI GAGTC 2 cut(s) 681, 814
PpuMI RGGWCCY 1 cut(s) 604
Psp5II RGGWCCY 1 cut(s) 604
PspFI CCCAGC 2 cut(s) 481, 589
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 604
PspPPI RGGWCCY 1 cut(s) 604
PstI CTGCAG 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 382
PsuI RGATCY 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 331
RsaI GTAC 2 cut(s) 367, 412
RsaNI GTAC 2 cut(s) 366, 411
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 1 cut(s) 719
SatI GCNGC 1 cut(s) 329
Sau3AI GATC 4 cut(s) 25, 128, 725, 800
Sau96I GGNCC 1 cut(s) 604
SchI GAGTC 2 cut(s) 681, 815
SduI GDGCHC 1 cut(s) 625
SfcI CTRYAG 2 cut(s) 67, 112
SfuI TTCGAA 1 cut(s) 791
SinI GGWCC 1 cut(s) 604
SmiMI CAYNNNNRTG 1 cut(s) 242
SsiI CCGC 2 cut(s) 313, 386
SspI AATATT 1 cut(s) 715
SspMI CTAG 2 cut(s) 567, 841
TaaI ACNGT 2 cut(s) 415, 545
TaiI ACGT 1 cut(s) 786
TaqI TCGA 2 cut(s) 148, 791
TatI WGTACW 1 cut(s) 410
TfiI GAWTC 3 cut(s) 229, 295, 827
Tru1I TTAA 1 cut(s) 719
Tru9I TTAA 1 cut(s) 719
TscAI CASTG 2 cut(s) 420, 684
TseI GCWGC 1 cut(s) 328
TspDTI ATGAA 7 cut(s) 124, 152, 219, 232, 340, 368, 686
TspGWI ACGGA 2 cut(s) 232, 678
TspRI CASTG 2 cut(s) 420, 684
VpaK11BI GGWCC 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XspI CTAG 2 cut(s) 567, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.