Rroxscaffold_1G00033660

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016250
Physical Location & Seq
Reverse (-)
48777790 .. 48786513
8724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033660.1

Sequence Viewer

Length: 1785 bp
ATGCTACTATACTTAGTGCATGTGCAAGTATACATGATCTTGAATGGGGTAGCCATTTGCATGCTCGGATACTCCGTATGGGAACCAAGTATGGATGTTTTTGCAGGTAGTGGGCTGATTGATATGTATGTGAAATGTGGACAGTTGCATCTTGCTAGGCAGGTGTTTGACAACATGACAGAACACAATGCAGTTTCTTGGACTTCTATGATCAGTGGAGCCATGCAGTTTGGGCTTGAAGATGAAGCTTTAGTACTGTTTAACCGAATGAGAAAATCTCCTATTGCCTTGGATGAGTTTACTCTTGCAACAGTTCTTGGGGTTTGTTCAGGCCATGAGCATGCTTGGGTTGGGGAGCAGCTCCATGGATATACAATCAAAGGTGGAATGATCTCCTCTATTCCTGTAGGGAATGCTCTTGTTACGATGTATGCAAAATATGGAAACACTGATAAAGCCAGCCAAGCATTTGAGTTGATGCCTGCTAAAGATATAATATCATGGACAGCCATGATCACAGCATTCTCTCAAACTGGTAATGTTGAAAAATCCCGAGAGTATTTTGATAAAATGCCAGAGCGAAATGTCATAAGTTGGAACTCAATGTTAGGAACATATTTCCAAAATGATTTATGGGAAGAAGGCCTTAAATTGTATACTCTTATGCGAAGGGTAGGGGTTAACCTGGATTGGATCACACTAGTAACTTCAATCAGTGCCTGTGCTGATTTGGCAATATTGAAACTTGGGCTGCAGATTATAGCTCAAGCTGAAAAATTAGGATTTGGTTCTAATGTTTCAGTCATGAATAGCATTATTACTATGTATTCAAGATGTGGACAAATTATAGAAGCGAAAAGAGTGTTTGATTCAATATGTGATAAAAATTTGATTTCTTGGAATGCAATTATGGCTGGATATGCTCAAAATGGACAAGGTAGGAAGGTGATCGAGGTTTTTGAGAATATGTTGAAAATGGACTGCGCACCTGATCATATAAGCTATGTATCTGTTCTATCCGGTTGTGGCCATTCAGGGCTCATAACAGAGGGGAAACACTACTTCAGTTCTATGACTGAAGATTTTGGCATCAATCCTACATGTGAACATTTTGCTTGTATGGTAGATCTACTTGGTCGGGCTGGGTTACTGAAGGAAGCCAGGAATTTGATTGATGCAATGCCCTTTAAGCCAAATGCTGCTATCTGGGGAGCTCTTCTTGGTGCTTGCCGGCTTCATCGCGATTATAATTTAGCAGAATTTGCAGCAAGAAATTTGCTTGAATTAAATTTAGATGATTCTGGCAGTTATGTCCTTCTAGCTAATACATATTCAGATTGTGGACAACAAGAAGCTTTTGCAGATGTAAGAAAACAGATGAGAAAGAAAGGAATAGAGAAAAATCCTGGATGTAGCTGGATAGAGGTTAATAATAGGGTACACGTGTTCACTGTAGATGACACTAATCATCCACAAATCAAGGACGTTTATAGAATACTAGAGGAGATAGTCAAGAAGATAGAAGAAACAGGATGCTATGTCAATCCAACTAATGCAGTTCGTTCTCAGTGCCATCATAGTGAGAAGCTTGCTGTTGCTTTTGGGTTGCTTAGCTTGCCTGCTTGGATGCCTATCTATGTAATGAAAAATCTTCGAGTTTGCCATGATTGTCACTTGGTAATCAAGCTGATCTCCCTTGTTACCTCAAGGGAACTTATTGTGCGGGATGGACACCGGTTTCATCATTTCAAGGATGGATGTTGCTCCTGTGGAGACTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

594

Amino Acids

66.52

Weight (kDa)

6.13

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 63 - 109 2e-06 PPR repeat family
PPR PF01535 65 - 93 7.8e-06 PPR repeat
PPR PF01535 166 - 196 2e-06 PPR repeat
PPR_2 PF13041 194 - 242 2e-07 PPR repeat family
PPR_2 PF13041 296 - 343 2.6e-11 PPR repeat family
PPR PF01535 298 - 328 4.7e-06 PPR repeat
E_motif PF20431 413 - 475 6.3e-20 E motif
DYW_deaminase PF14432 523 - 594 4.3e-30 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016250)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00672 FvH4_3g26580
malus_domestica MD03G1153100.v1.1
prunus_persica Prupe.6G140100_v2.0.a1
pyrus_communis pycom03g10670
rosa_chinensis RchiOBHm_Chr5g0048221
rosa_laevigata RLG00000034503
rosa_multiflora Rmu_sc0001408.1_g000005
rosa_roxburghii Rroxscaffold_1G00033660
rosa_rugosa Rorug05G0237300
rosa_samantha Rh5AG316300 Rh5BG327000 Rh5CG353200 Rh5DG338100
rosa_wichuraiana Rw5G029650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1248
AarI CACCTGC 1 cut(s) 151
Acc16I TGCGCA 1 cut(s) 985
Acc36I ACCTGC 2 cut(s) 95, 151
AccI GTMKAC 2 cut(s) 30, 656
AccII CGCG 1 cut(s) 1242
AciI CCGC 1 cut(s) 1723
AclWI GGATC 1 cut(s) 701
AcoI YGGCCR 1 cut(s) 1027
AcsI RAATTY 5 cut(s) 886, 1165, 1259, 1273, 1288
AcuI CTGAAG 3 cut(s) 1048, 1098, 1172
AcvI CACGTG 1 cut(s) 1444
AfaI GTAC 2 cut(s) 255, 1440
AflIII ACRYGT 3 cut(s) 1100, 1441, 1443
AgeI ACCGGT 1 cut(s) 1734
AhlI ACTAGT 1 cut(s) 700
AjnI CCWGG 3 cut(s) 684, 1160, 1405
Alw21I GWGCWC 1 cut(s) 1216
Alw26I GTCTC 1 cut(s) 1767
AlwI GGATC 1 cut(s) 701
AlwNI CAGNNNCTG 1 cut(s) 720
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 3 cut(s) 331, 643, 1027
ApeKI GCWGC 4 cut(s) 358, 751, 1199, 1265
ApoI RAATTY 5 cut(s) 886, 1165, 1259, 1273, 1288
AsiGI ACCGGT 1 cut(s) 1734
AspLEI GCGC 1 cut(s) 986
AsuHPI GGTGA 1 cut(s) 958
AvaI CYCGRG 1 cut(s) 552
BaeI ACNNNNGTAYC 2 cut(s) 1430, 1463
BalI TGGCCA 1 cut(s) 1029
BanII GRGCYC 2 cut(s) 1041, 1216
BarI GAAGNNNNNNTAC 2 cut(s) 237, 269
BbrPI CACGTG 1 cut(s) 1444
Bbv12I GWGCWC 1 cut(s) 1216
BbvI GCAGC 4 cut(s) 370, 738, 1186, 1277
BccI CCATC 3 cut(s) 1581, 1721, 1748
BciT130I CCWGG 3 cut(s) 686, 1162, 1407
BciVI GTATCC 1 cut(s) 62
BclI TGATCA 3 cut(s) 210, 513, 991
BcoDI GTCTC 1 cut(s) 1767
BcuI ACTAGT 1 cut(s) 700
BfaI CTAG 4 cut(s) 156, 701, 1319, 1499
BfmI CTRYAG 3 cut(s) 405, 752, 1452
BfuAI ACCTGC 2 cut(s) 95, 151
BfuI GTATCC 1 cut(s) 62
BglII AGATCT 1 cut(s) 1126
BisI GCNGC 4 cut(s) 359, 752, 1200, 1266
BlpI GCTNAGC 1 cut(s) 1610
BlsI GCNGC 4 cut(s) 360, 753, 1201, 1267
BmcAI AGTACT 1 cut(s) 255
Bme1390I CCNGG 3 cut(s) 686, 1162, 1407
BmeT110I CYCGRG 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 84, 220
BmrFI CCNGG 3 cut(s) 686, 1162, 1407
BmsI GCATC 6 cut(s) 157, 468, 1098, 1165, 1523, 1617
BplI GAGNNNNNCTC 2 cut(s) 262, 294
Bpu1102I GCTNAGC 1 cut(s) 1610
BpuEI CTTGAG 2 cut(s) 750, 1690
BsaAI YACGTR 1 cut(s) 1444
BsaBI GATNNNNATC 1 cut(s) 1631
BsaJI CCNNGG 2 cut(s) 288, 364
BsaWI WCCGGW 2 cut(s) 1019, 1734
Bse118I RCCGGY 2 cut(s) 1230, 1734
Bse1I ACTGG 2 cut(s) 538, 1784
Bse3DI GCAATG 1 cut(s) 1185
Bse8I GATNNNNATC 1 cut(s) 1631
BseBI CCWGG 3 cut(s) 686, 1162, 1407
BseDI CCNNGG 2 cut(s) 288, 364
BseGI GGATG 9 cut(s) 100, 298, 1415, 1468, 1538, 1632, 1732, 1759, 1763
BseJI GATNNNNATC 1 cut(s) 1631
BseMI GCAATG 1 cut(s) 1185
BseMII CTCAG 1 cut(s) 1580
BseNI ACTGG 2 cut(s) 538, 1784
BseRI GAGGAG 2 cut(s) 385, 1517
BseXI GCAGC 4 cut(s) 370, 738, 1186, 1277
BseYI CCCAGC 1 cut(s) 1142
Bsh1236I CGCG 1 cut(s) 1242
BshFI GGCC 3 cut(s) 333, 645, 1029
BshTI ACCGGT 1 cut(s) 1734
BsiHKAI GWGCWC 1 cut(s) 1216
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 3 cut(s) 1020, 1231, 1735
BsmAI GTCTC 1 cut(s) 1767
BsmI GAATGC 3 cut(s) 418, 521, 907
BsnI GGCC 3 cut(s) 333, 645, 1029
BsoBI CYCGRG 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 1041, 1216
Bsp143I GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
Bsp1720I GCTNAGC 1 cut(s) 1610
Bsp19I CCATGG 1 cut(s) 364
Bsp68I TCGCGA 1 cut(s) 1242
BspACI CCGC 1 cut(s) 1723
BspANI GGCC 3 cut(s) 333, 645, 1029
BspCNI CTCAG 1 cut(s) 1579
BspFNI CGCG 1 cut(s) 1242
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 2 cut(s) 84, 220
BspMAI CTGCAG 1 cut(s) 756
BspMI ACCTGC 2 cut(s) 95, 151
BspPI GGATC 1 cut(s) 701
BspQI GCTCTTC 1 cut(s) 1221
BsrDI GCAATG 1 cut(s) 1185
BsrFI RCCGGY 2 cut(s) 1230, 1734
BsrI ACTGG 2 cut(s) 538, 1784
BssAI RCCGGY 2 cut(s) 1230, 1734
BssECI CCNNGG 2 cut(s) 288, 364
BssMI GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
BssNAI GTATAC 2 cut(s) 31, 657
BssT1I CCWWGG 2 cut(s) 288, 364
Bst1107I GTATAC 2 cut(s) 31, 657
Bst2UI CCWGG 3 cut(s) 686, 1162, 1407
Bst4CI ACNGT 4 cut(s) 144, 258, 313, 1453
Bst6I CTCTTC 1 cut(s) 1221
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstBAI YACGTR 1 cut(s) 1444
BstC8I GCNNGC 9 cut(s) 62, 342, 460, 483, 1228, 1232, 1590, 1616, 1620
BstDEI CTNAG 3 cut(s) 13, 1566, 1610
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 9 cut(s) 100, 298, 1415, 1468, 1538, 1632, 1732, 1759, 1763
BstFNI CGCG 1 cut(s) 1242
BstHHI GCGC 1 cut(s) 986
BstKTI GATC 9 cut(s) 39, 213, 393, 516, 696, 951, 994, 1129, 1692
BstMAI GTCTC 1 cut(s) 1767
BstMBI GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
BstMWI GCNNNNNNNGC 8 cut(s) 232, 464, 731, 911, 920, 1189, 1262, 1615
BstNI CCWGG 3 cut(s) 686, 1162, 1407
BstNSI RCATGY 4 cut(s) 23, 64, 344, 1104
BstSCI CCNGG 3 cut(s) 684, 1160, 1405
BstSFI CTRYAG 3 cut(s) 405, 752, 1452
BstUI CGCG 1 cut(s) 1242
BstV1I GCAGC 4 cut(s) 370, 738, 1186, 1277
BstX2I RGATCY 1 cut(s) 1126
BstYI RGATCY 1 cut(s) 1126
BstZ17I GTATAC 2 cut(s) 31, 657
BsuI GTATCC 1 cut(s) 62
BsuRI GGCC 3 cut(s) 333, 645, 1029
BtgI CCRYGG 1 cut(s) 364
BtgZI GCGATG 1 cut(s) 1223
BtsCI GGATG 9 cut(s) 100, 298, 1415, 1468, 1538, 1632, 1732, 1759, 1763
BtsIMutI CAGTG 5 cut(s) 220, 447, 721, 1449, 1574
BtuMI TCGCGA 1 cut(s) 1242
BveI ACCTGC 2 cut(s) 95, 151
Cac8I GCNNGC 9 cut(s) 62, 342, 460, 483, 1228, 1232, 1590, 1616, 1620
CaiI CAGNNNCTG 1 cut(s) 720
CciI TCATGA 1 cut(s) 804
CfoI GCGC 1 cut(s) 986
Cfr10I RCCGGY 2 cut(s) 1230, 1734
Csp6I GTAC 2 cut(s) 254, 1439
CspAI ACCGGT 1 cut(s) 1734
CviQI GTAC 2 cut(s) 254, 1439
DdeI CTNAG 3 cut(s) 13, 1566, 1610
DpnI GATC 9 cut(s) 38, 212, 392, 515, 695, 950, 993, 1128, 1691
DpnII GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
EaeI YGGCCR 1 cut(s) 1027
Eam1104I CTCTTC 1 cut(s) 1221
EarI CTCTTC 1 cut(s) 1221
Ecl136II GAGCTC 1 cut(s) 1214
Eco130I CCWWGG 2 cut(s) 288, 364
Eco147I AGGCCT 1 cut(s) 645
Eco24I GRGCYC 2 cut(s) 1041, 1216
Eco53kI GAGCTC 1 cut(s) 1214
Eco57I CTGAAG 3 cut(s) 1048, 1098, 1172
Eco72I CACGTG 1 cut(s) 1444
Eco88I CYCGRG 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 1214
EcoRII CCWGG 3 cut(s) 684, 1160, 1405
EcoT14I CCWWGG 2 cut(s) 288, 364
EcoT38I GRGCYC 2 cut(s) 1041, 1216
ErhI CCWWGG 2 cut(s) 288, 364
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FauI CCCGC 1 cut(s) 1716
FbaI TGATCA 3 cut(s) 210, 513, 991
FblI GTMKAC 2 cut(s) 30, 656
Fnu4HI GCNGC 4 cut(s) 359, 752, 1200, 1266
FokI GGATG 9 cut(s) 107, 305, 1422, 1455, 1545, 1639, 1739, 1766, 1770
FriOI GRGCYC 2 cut(s) 1041, 1216
Fsp4HI GCNGC 4 cut(s) 359, 752, 1200, 1266
FspBI CTAG 4 cut(s) 156, 701, 1319, 1499
FspI TGCGCA 1 cut(s) 985
GlaI GCGC 1 cut(s) 985
GluI GCNGC 4 cut(s) 359, 752, 1200, 1266
GsaI CCCAGC 1 cut(s) 1146
HaeIII GGCC 3 cut(s) 333, 645, 1029
HapII CCGG 3 cut(s) 1020, 1231, 1735
HhaI GCGC 1 cut(s) 986
Hin6I GCGC 1 cut(s) 984
HinP1I GCGC 1 cut(s) 984
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HindIII AAGCTT 3 cut(s) 246, 1353, 1586
HinfI GANTC 2 cut(s) 869, 1298
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 3 cut(s) 1020, 1231, 1735
HphI GGTGA 1 cut(s) 958
Hpy188I TCNGA 2 cut(s) 68, 1336
Hpy188III TCNNGA 6 cut(s) 40, 552, 805, 831, 1241, 1513
HpyAV CCTTC 5 cut(s) 635, 663, 937, 1147, 1325
HpyCH4III ACNGT 4 cut(s) 144, 258, 313, 1453
HpyCH4IV ACGT 2 cut(s) 1443, 1485
HpyF10VI GCNNNNNNNGC 8 cut(s) 232, 464, 731, 911, 920, 1189, 1262, 1615
HpyF3I CTNAG 3 cut(s) 13, 1566, 1610
HpySE526I ACGT 2 cut(s) 1443, 1485
HspAI GCGC 1 cut(s) 984
KroI GCCGGC 1 cut(s) 1230
KroNI GCCGGC 1 cut(s) 1232
Ksp22I TGATCA 3 cut(s) 210, 513, 991
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
LguI GCTCTTC 1 cut(s) 1221
LmnI GCTCC 5 cut(s) 218, 355, 366, 1211, 1769
Lsp1109I GCAGC 4 cut(s) 370, 738, 1186, 1277
LweI GCATC 6 cut(s) 157, 468, 1098, 1165, 1523, 1617
MaeI CTAG 4 cut(s) 156, 701, 1319, 1499
MaeII ACGT 2 cut(s) 1443, 1485
MaeIII GTNAC 5 cut(s) 421, 703, 1146, 1670, 1699
MalI GATC 9 cut(s) 38, 212, 392, 515, 695, 950, 993, 1128, 1691
MboI GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
MboII GAAGA 7 cut(s) 251, 650, 1091, 1208, 1528, 1535, 1643
MflI RGATCY 1 cut(s) 1126
MhlI GDGCHC 2 cut(s) 1041, 1216
MlsI TGGCCA 1 cut(s) 1029
MluNI TGGCCA 1 cut(s) 1029
MmeI TCCRAC 2 cut(s) 575, 1571
MnlI CCTC 6 cut(s) 406, 946, 1042, 1417, 1495, 1714
Mox20I TGGCCA 1 cut(s) 1029
MroNI GCCGGC 1 cut(s) 1230
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 6 cut(s) 261, 648, 681, 1188, 1286, 1428
MslI CAYNNNNRTG 3 cut(s) 59, 339, 1578
Msp20I TGGCCA 1 cut(s) 1029
MspI CCGG 3 cut(s) 1020, 1231, 1735
MspR9I CCNGG 3 cut(s) 686, 1162, 1407
Mva1269I GAATGC 3 cut(s) 418, 521, 907
MvaI CCWGG 3 cut(s) 686, 1162, 1407
MvnI CGCG 1 cut(s) 1242
MwoI GCNNNNNNNGC 8 cut(s) 232, 464, 731, 911, 920, 1189, 1262, 1615
NaeI GCCGGC 1 cut(s) 1232
NcoI CCATGG 1 cut(s) 364
NdeII GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
NgoMIV GCCGGC 1 cut(s) 1230
NlaIV GGNNCC 2 cut(s) 84, 220
NmuCI GTSAC 1 cut(s) 1670
NruI TCGCGA 1 cut(s) 1242
NsbI TGCGCA 1 cut(s) 985
NspI RCATGY 4 cut(s) 23, 64, 344, 1104
PaeI GCATGC 2 cut(s) 64, 344
PagI TCATGA 1 cut(s) 804
PaqCI CACCTGC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 645
PciI ACATGT 1 cut(s) 1100
PciSI GCTCTTC 1 cut(s) 1221
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 3 cut(s) 418, 521, 907
PdiI GCCGGC 1 cut(s) 1232
PfeI GAWTC 2 cut(s) 869, 1298
PfoI TCCNGGA 1 cut(s) 1405
PinAI ACCGGT 1 cut(s) 1734
PkrI GCNGC 4 cut(s) 360, 753, 1201, 1267
PmaCI CACGTG 1 cut(s) 1444
PmlI CACGTG 1 cut(s) 1444
Ppu21I YACGTR 1 cut(s) 1444
PscI ACATGT 1 cut(s) 1100
PsiI TTATAA 1 cut(s) 1248
Psp124BI GAGCTC 1 cut(s) 1216
Psp6I CCWGG 3 cut(s) 684, 1160, 1405
PspCI CACGTG 1 cut(s) 1444
PspFI CCCAGC 1 cut(s) 1142
PspGI CCWGG 3 cut(s) 684, 1160, 1405
PspN4I GGNNCC 2 cut(s) 84, 220
PstI CTGCAG 1 cut(s) 756
PstNI CAGNNNCTG 1 cut(s) 720
PsuI RGATCY 1 cut(s) 1126
RruI TCGCGA 1 cut(s) 1242
RsaI GTAC 2 cut(s) 255, 1440
RsaNI GTAC 2 cut(s) 254, 1439
RseI CAYNNNNRTG 3 cut(s) 59, 339, 1578
SacI GAGCTC 1 cut(s) 1216
SapI GCTCTTC 1 cut(s) 1221
SaqAI TTAA 6 cut(s) 261, 648, 681, 1188, 1286, 1428
SatI GCNGC 4 cut(s) 359, 752, 1200, 1266
Sau3AI GATC 9 cut(s) 36, 210, 390, 513, 693, 948, 991, 1126, 1689
ScaI AGTACT 1 cut(s) 255
ScrFI CCNGG 3 cut(s) 686, 1162, 1407
SduI GDGCHC 2 cut(s) 1041, 1216
SfaNI GCATC 6 cut(s) 157, 468, 1098, 1165, 1523, 1617
SfcI CTRYAG 3 cut(s) 405, 752, 1452
SmiMI CAYNNNNRTG 3 cut(s) 59, 339, 1578
SmlI CTYRAG 2 cut(s) 765, 1705
SmoI CTYRAG 2 cut(s) 765, 1705
SpeI ACTAGT 1 cut(s) 700
SphI GCATGC 2 cut(s) 64, 344
SseBI AGGCCT 1 cut(s) 645
SsiI CCGC 1 cut(s) 1723
SspI AATATT 1 cut(s) 738
SspMI CTAG 4 cut(s) 156, 701, 1319, 1499
SstI GAGCTC 1 cut(s) 1216
StuI AGGCCT 1 cut(s) 645
StyD4I CCNGG 3 cut(s) 684, 1160, 1405
StyI CCWWGG 2 cut(s) 288, 364
TaaI ACNGT 4 cut(s) 144, 258, 313, 1453
TaiI ACGT 2 cut(s) 1446, 1488
TaqI TCGA 2 cut(s) 951, 1654
TatI WGTACW 1 cut(s) 253
TfiI GAWTC 2 cut(s) 869, 1298
Tru1I TTAA 6 cut(s) 261, 648, 681, 1188, 1286, 1428
Tru9I TTAA 6 cut(s) 261, 648, 681, 1188, 1286, 1428
TscAI CASTG 5 cut(s) 220, 454, 721, 1456, 1574
TseFI GTSAC 1 cut(s) 1670
TseI GCWGC 4 cut(s) 358, 751, 1199, 1265
Tsp45I GTSAC 1 cut(s) 1670
TspDTI ATGAA 5 cut(s) 258, 821, 1226, 1658, 1730
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 5 cut(s) 220, 454, 721, 1456, 1574
XapI RAATTY 5 cut(s) 886, 1165, 1259, 1273, 1288
XceI RCATGY 4 cut(s) 23, 64, 344, 1104
XmiI GTMKAC 2 cut(s) 30, 656
XspI CTAG 4 cut(s) 156, 701, 1319, 1499
ZrmI AGTACT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.