Rh5CG353200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0016250
Physical Location & Seq
Forward (+)
40997792 .. 41001038
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG353200.1

Sequence Viewer

Length: 2730 bp
ATGATGTCAATTCATCATCCATTGAAGCATCTCCGGATTTTTTCAGGCCTAATGGTTCGCTGTATCTCAACCCAAGAAAATATTTTGTCGAACCTTATTCTCTGCCACGATCCCTCCATGGAACAATCTTACATGCAATTGTCACAAAAATTCTATGAATCCATGAAAGCTTGTGCATCTCTAAGGTCGATCCCCATTGCCCGAAAACTCCATGGCCAACTCATTTCTGTAGGATTGGACTCTGCCATTTTCCTTCAGAACCATCTCTTGCATATGTATTCCCGTTGCAGCTTGATAGATGATGCTCGCTGGATTTTCTTCAGTATTCAACATCCTAATGTGTTTAGTTGGAATACAATGATTAGCGCGTTGGTAGACTTGGGTCACATGGGGGAGGCAGAGAAATTGTTCAATGAAATGCCTGAGAGAGACTCCGTCTCTTGGACTACAATGATGTCTGGCCATTTTCATAATGGGCAACCTAAGGATGCAATTAAAGTGTTTGCTTCAATGGTATGGAATTGTGATAGTTTCTATGACCCATTTTCCTTCTCTTGCGTAATGAAGGCTTGTGGTAGTCTTGGTCACATTAAGTTGGCTCTTCAGTTGCATACTCTAGTGGAGAAATTAAATTTTGGAGGCAACATGGTTATTCAAAATTCTATAATTGACATGTACATCAAGTGTGGTGCACTGCGTTTGGCTGAGAGAATGTTTATGAGGATCCCTAATCCTAGTTTGTTTTGTTGGAACAGTATGATCTATGGCTGCTCTAAACTTTATGGGGTCAGAAGGGCTCTCCATATGTTCATTGAAATGCCTGAACGTGATTGTGTGTCTTGGAATACCATAATATCAATCTTTTCTCAACATGGTTTTGGGGTTGAGAGTCTTAGTATGTTTGTTGAGATGTGGAACCATGGTTTTAGACCAAATGCTATGATATATGCTACTATACTTAGTGCATGTGCAAGTATACATGATCTTGAATGGGGTAGCCATTTGCATGCTCGGATACTCCGTATGGAACCAAGTATGGATGTTTTTGCAGGTAGTGGGCTGATTGATATGTATGTGAAATGTGGACAGTTGAATCTTGCTAGGCAAGTGTTTGACAACATGACAGAACACAATGCAGTTTCTTGGACTTCTATGATCAGTGGAGCCATGCAGTTTGGGCTTGAAGATGAAGCTTTAGTACTGTTTAACCGAATGAGAAAATCTCCTATTGCCTTGGATGAGTGTACTCTTGCAACAGTTCTTGGGGTTTGTTCAGGCCATGAGCATGCTTGGGTTGGGGAGCAGCTCCATGGATATACAATCAAAGGTGGAATGATCTCCTCTATTCCTGTAGGGAATGCTCTTGTTACGATGTATGCAAAATGTGGAAACTCTGATAAAGCCAGCCAAGCATTTGAGTTGATGCCTGCTAAAGATATAATATCATGGACAGCCATGATCACAGCATTCTCTCAAACTGGTAATGTTGAAAAATCCCGAGAGTATTTTGATAAAATGCCAGCGCGAAATGTCATAAGTTGGAACTCAATGTTAGGAACATATTTCCAAAATGGTTTATGGGAAGAAGGCCTTAAATTGTATACACTTATGCGAAGGGTAGGGGTTAACCTGGATTGGATCACACTAGTAACTTCAATCAGTGCCTGTGCTGATTTGGCAATATTGAAACTTGGGCTGCAGATTATAGCTCAAGCTGAAAAATTAGGATTTGGTTCTAATGTTTCAGTCATGAATAGCATTATTACTATGTATTCAAGATGTGGACGAATTATAGAAGCGAAAAGAGTGTTTGATTCAATATGTGATAAAAATTTGATTTCTTGGAATGCAATTATGGCTGGATATGCTCAAAATGGACAAGGTAGGAAGGTGATTGAGGTTTTTGAGAATATGTTGAAAATGGACTGCGCACCTGATCATATAAGCTATGTATCTGTTCTATCAGGTTGTGGCCATTCAGGGCTAGTAACAGAGGGGAAACACTACTTCAGTTCTATGACTGAAGATTTTGGCATCAATCCTACATGTGAACATTTTGCTTGTATGGTAGATCTACTTGGTCGGGCTGGGTTACTGAAGGAGGCCAGGAATTTGATTGATGCAATGCCCTTTAAGCCAAATGCTGCTATCTGGGGAGCTCTTCTTGGTGCTTGCCGGCTTCATCGCGATTATAATTTAGCGGAATTTGCAGCAAGAAATTTGCTTGAATTAGATTTAGATGATTCTGGTAGTTATGTCCTTCTAGCTAATACATATTCAGATTGTGGACAACTAGAAGCTTTTGCAGATGTAAGAAAACAGATGAGAAAGAAAGGAATAGAGAAAAATCCTGGATGTAGCTGGATAGAGGTTAATAATAGGGTACACGTGTTCACTGTAGATGACACTAATCATCCACAAATCAAGGACGTTTATAGAATACTAGAGGAGATAGTCAAGAAGATAGAAGATACAGGATGCTATGTCAATCCAACTAATGCAGTTCGTTCTCAGTGCCATCACAGTGAGAAGCTTGCTGTTGCTTTTGGGTTGCTTAGCTTGCCTGCTTGGATGCCTATCTATGTAATGAAAAATCTTCGAGTTTGCCATGATTGTCACTTGGTAATCAAGCTGATCTCCCTTGTTACCTCAAGGGAACTTATTGTGCGGGATGGACACCGGTTTCATCATTTCAAGGATGGATGTTGCTCCTGTGGAGACTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

909

Amino Acids

102.35

Weight (kDa)

6.76

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 111 - 140 4.4e-06 PPR repeat
PPR_2 PF13041 112 - 143 3.9e-08 PPR repeat family
PPR PF01535 115 - 143 2.7e-07 PPR repeat
PPR_2 PF13041 144 - 191 9.3e-07 PPR repeat family
PPR_2 PF13041 509 - 557 5.8e-08 PPR repeat family
PPR_2 PF13041 611 - 658 4.5e-11 PPR repeat family
PPR PF01535 613 - 643 7.7e-06 PPR repeat
E_motif PF20431 728 - 790 2e-19 E motif
DYW_deaminase PF14432 838 - 909 7.7e-30 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016250)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00672 FvH4_3g26580
malus_domestica MD03G1153100.v1.1
prunus_persica Prupe.6G140100_v2.0.a1
pyrus_communis pycom03g10670
rosa_chinensis RchiOBHm_Chr5g0048221
rosa_laevigata RLG00000034503
rosa_multiflora Rmu_sc0001408.1_g000005
rosa_roxburghii Rroxscaffold_1G00033660
rosa_rugosa Rorug05G0237300
rosa_samantha Rh5AG316300 Rh5BG327000 Rh5CG353200 Rh5DG338100
rosa_wichuraiana Rw5G029650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2193
Acc16I TGCGCA 1 cut(s) 1930
Acc36I ACCTGC 1 cut(s) 1040
AccI GTMKAC 3 cut(s) 375, 976, 1601
AccII CGCG 3 cut(s) 368, 1525, 2187
AccIII TCCGGA 1 cut(s) 33
AciI CCGC 2 cut(s) 2201, 2668
AclWI GGATC 5 cut(s) 104, 184, 718, 731, 1646
AcoI YGGCCR 3 cut(s) 214, 460, 1972
AcsI RAATTY 7 cut(s) 149, 631, 658, 1831, 2110, 2204, 2218
AcuI CTGAAG 6 cut(s) 239, 304, 587, 1993, 2043, 2117
AcvI CACGTG 1 cut(s) 2389
AfaI GTAC 4 cut(s) 677, 1200, 1246, 2385
AfiI CCNNNNNNNGG 1 cut(s) 441
AflIII ACRYGT 4 cut(s) 672, 2045, 2386, 2388
AgeI ACCGGT 1 cut(s) 2679
AhlI ACTAGT 1 cut(s) 1645
AjnI CCWGG 3 cut(s) 1629, 2105, 2350
Alw21I GWGCWC 2 cut(s) 694, 2161
Alw26I GTCTC 3 cut(s) 423, 442, 2712
Alw44I GTGCAC 1 cut(s) 690
AlwI GGATC 5 cut(s) 104, 184, 718, 731, 1646
AlwNI CAGNNNCTG 1 cut(s) 1665
Ama87I CYCGRG 1 cut(s) 1497
Aor13HI TCCGGA 1 cut(s) 33
AoxI GGCC 7 cut(s) 46, 214, 460, 1276, 1588, 1972, 2103
ApaLI GTGCAC 1 cut(s) 690
ApeKI GCWGC 6 cut(s) 288, 768, 1303, 1696, 2144, 2210
ApoI RAATTY 7 cut(s) 149, 631, 658, 1831, 2110, 2204, 2218
AsiGI ACCGGT 1 cut(s) 2679
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 3 cut(s) 368, 1525, 1931
AsuHPI GGTGA 1 cut(s) 1903
AvaI CYCGRG 1 cut(s) 1497
AxyI CCTNAGG 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 694
BaeI ACNNNNGTAYC 2 cut(s) 2375, 2408
BalI TGGCCA 3 cut(s) 216, 462, 1974
BamHI GGATCC 1 cut(s) 723
BanII GRGCYC 2 cut(s) 799, 2161
BarI GAAGNNNNNNTAC 2 cut(s) 1182, 1214
BbrPI CACGTG 1 cut(s) 2389
Bbv12I GWGCWC 2 cut(s) 694, 2161
BbvI GCAGC 6 cut(s) 300, 755, 1315, 1683, 2131, 2222
BccI CCATC 4 cut(s) 270, 2526, 2666, 2693
BciT130I CCWGG 3 cut(s) 1631, 2107, 2352
BciVI GTATCC 1 cut(s) 1008
BclI TGATCA 3 cut(s) 1155, 1458, 1936
BcoDI GTCTC 3 cut(s) 423, 442, 2712
BcuI ACTAGT 1 cut(s) 1645
BfaI CTAG 8 cut(s) 617, 735, 1101, 1646, 1985, 2264, 2294, 2444
BfmI CTRYAG 4 cut(s) 228, 1350, 1697, 2397
BfuAI ACCTGC 1 cut(s) 1040
BfuI GTATCC 1 cut(s) 1008
BglII AGATCT 1 cut(s) 2071
BisI GCNGC 6 cut(s) 289, 769, 1304, 1697, 2145, 2211
BlpI GCTNAGC 1 cut(s) 2555
BlsI GCNGC 6 cut(s) 290, 770, 1305, 1698, 2146, 2212
BmcAI AGTACT 1 cut(s) 1200
Bme1390I CCNGG 3 cut(s) 1631, 2107, 2352
BmeT110I CYCGRG 1 cut(s) 1497
BmiI GGNNCC 4 cut(s) 725, 917, 1029, 1165
BmrFI CCNGG 3 cut(s) 1631, 2107, 2352
BmsI GCATC 9 cut(s) 37, 185, 292, 478, 1413, 2043, 2110, 2468, 2562
BoxI GACNNNNGTC 1 cut(s) 381
BplI GAGNNNNNCTC 4 cut(s) 416, 448, 1207, 1239
Bpu1102I GCTNAGC 1 cut(s) 2555
BpuEI CTTGAG 2 cut(s) 1695, 2635
BsaAI YACGTR 1 cut(s) 2389
BsaBI GATNNNNATC 1 cut(s) 2576
BsaJI CCNNGG 5 cut(s) 117, 211, 919, 1233, 1309
BsaWI WCCGGW 2 cut(s) 33, 2679
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 441
Bse118I RCCGGY 2 cut(s) 2175, 2679
Bse1I ACTGG 2 cut(s) 1483, 2729
Bse21I CCTNAGG 1 cut(s) 483
Bse3DI GCAATG 2 cut(s) 195, 2130
Bse8I GATNNNNATC 1 cut(s) 2576
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 3 cut(s) 1631, 2107, 2352
BseDI CCNNGG 5 cut(s) 117, 211, 919, 1233, 1309
BseJI GATNNNNATC 1 cut(s) 2576
BseLI CCNNNNNNNGG 1 cut(s) 441
BseMI GCAATG 2 cut(s) 195, 2130
BseMII CTCAG 3 cut(s) 414, 696, 2525
BseNI ACTGG 2 cut(s) 1483, 2729
BseRI GAGGAG 2 cut(s) 1330, 2462
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 6 cut(s) 300, 755, 1315, 1683, 2131, 2222
BseYI CCCAGC 1 cut(s) 2087
Bsh1236I CGCG 3 cut(s) 368, 1525, 2187
BshFI GGCC 7 cut(s) 48, 216, 462, 1278, 1590, 1974, 2105
BshTI ACCGGT 1 cut(s) 2679
BsiHKAI GWGCWC 2 cut(s) 694, 2161
BsiHKCI CYCGRG 1 cut(s) 1497
BsiSI CCGG 3 cut(s) 34, 2176, 2680
BslI CCNNNNNNNGG 1 cut(s) 441
BsmAI GTCTC 3 cut(s) 423, 442, 2712
BsmBI CGTCTC 1 cut(s) 442
BsmI GAATGC 3 cut(s) 1363, 1466, 1852
BsnI GGCC 7 cut(s) 48, 216, 462, 1278, 1590, 1974, 2105
BsoBI CYCGRG 1 cut(s) 1497
Bsp1286I GDGCHC 3 cut(s) 694, 799, 2161
Bsp13I TCCGGA 1 cut(s) 33
Bsp1407I TGTACA 1 cut(s) 675
Bsp1720I GCTNAGC 1 cut(s) 2555
Bsp19I CCATGG 4 cut(s) 117, 211, 919, 1309
Bsp68I TCGCGA 1 cut(s) 2187
BspACI CCGC 2 cut(s) 2201, 2668
BspANI GGCC 7 cut(s) 48, 216, 462, 1278, 1590, 1974, 2105
BspCNI CTCAG 3 cut(s) 415, 697, 2524
BspEI TCCGGA 1 cut(s) 33
BspFNI CGCG 3 cut(s) 368, 1525, 2187
BspHI TCATGA 1 cut(s) 1749
BspLI GGNNCC 4 cut(s) 725, 917, 1029, 1165
BspMAI CTGCAG 1 cut(s) 1701
BspMI ACCTGC 1 cut(s) 1040
BspPI GGATC 5 cut(s) 104, 184, 718, 731, 1646
BspQI GCTCTTC 2 cut(s) 606, 2166
BsrDI GCAATG 2 cut(s) 195, 2130
BsrFI RCCGGY 2 cut(s) 2175, 2679
BsrGI TGTACA 1 cut(s) 675
BsrI ACTGG 2 cut(s) 1483, 2729
BssAI RCCGGY 2 cut(s) 2175, 2679
BssECI CCNNGG 5 cut(s) 117, 211, 919, 1233, 1309
BssNAI GTATAC 2 cut(s) 977, 1602
BssT1I CCWWGG 5 cut(s) 117, 211, 919, 1233, 1309
Bst1107I GTATAC 2 cut(s) 977, 1602
Bst2UI CCWGG 3 cut(s) 1631, 2107, 2352
Bst4CI ACNGT 6 cut(s) 755, 1089, 1203, 1258, 2398, 2525
Bst6I CTCTTC 2 cut(s) 606, 2166
BstAUI TGTACA 1 cut(s) 675
BstBAI YACGTR 1 cut(s) 2389
BstDEI CTNAG 8 cut(s) 182, 423, 483, 705, 893, 959, 2511, 2555
BstDSI CCRYGG 4 cut(s) 117, 211, 919, 1309
BstFNI CGCG 3 cut(s) 368, 1525, 2187
BstHHI GCGC 3 cut(s) 368, 1525, 1931
BstMAI GTCTC 3 cut(s) 423, 442, 2712
BstMWI GCNNNNNNNGC 8 cut(s) 1177, 1409, 1676, 1856, 1865, 2134, 2207, 2560
BstNI CCWGG 3 cut(s) 1631, 2107, 2352
BstNSI RCATGY 6 cut(s) 136, 676, 969, 1010, 1289, 2049
BstPAI GACNNNNGTC 1 cut(s) 381
BstSCI CCNGG 3 cut(s) 1629, 2105, 2350
BstSFI CTRYAG 4 cut(s) 228, 1350, 1697, 2397
BstSLI GKGCMC 1 cut(s) 694
BstUI CGCG 3 cut(s) 368, 1525, 2187
BstV1I GCAGC 6 cut(s) 300, 755, 1315, 1683, 2131, 2222
BstX2I RGATCY 2 cut(s) 723, 2071
BstYI RGATCY 2 cut(s) 723, 2071
BstZ17I GTATAC 2 cut(s) 977, 1602
Bsu36I CCTNAGG 1 cut(s) 483
BsuI GTATCC 1 cut(s) 1008
BsuRI GGCC 7 cut(s) 48, 216, 462, 1278, 1590, 1974, 2105
BtgI CCRYGG 4 cut(s) 117, 211, 919, 1309
BtgZI GCGATG 1 cut(s) 2168
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 6 cut(s) 692, 1165, 1666, 2394, 2519, 2530
BtuMI TCGCGA 1 cut(s) 2187
BveI ACCTGC 1 cut(s) 1040
CaiI CAGNNNCTG 1 cut(s) 1665
CciI TCATGA 1 cut(s) 1749
CfoI GCGC 3 cut(s) 368, 1525, 1931
Cfr10I RCCGGY 2 cut(s) 2175, 2679
Csp6I GTAC 4 cut(s) 676, 1199, 1245, 2384
CspAI ACCGGT 1 cut(s) 2679
CviQI GTAC 4 cut(s) 676, 1199, 1245, 2384
DdeI CTNAG 8 cut(s) 182, 423, 483, 705, 893, 959, 2511, 2555
EaeI YGGCCR 3 cut(s) 214, 460, 1972
Eam1104I CTCTTC 2 cut(s) 606, 2166
EarI CTCTTC 2 cut(s) 606, 2166
Ecl136II GAGCTC 1 cut(s) 2159
Eco130I CCWWGG 5 cut(s) 117, 211, 919, 1233, 1309
Eco147I AGGCCT 2 cut(s) 48, 1590
Eco24I GRGCYC 2 cut(s) 799, 2161
Eco53kI GAGCTC 1 cut(s) 2159
Eco57I CTGAAG 6 cut(s) 239, 304, 587, 1993, 2043, 2117
Eco72I CACGTG 1 cut(s) 2389
Eco81I CCTNAGG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 1497
EcoICRI GAGCTC 1 cut(s) 2159
EcoRII CCWGG 3 cut(s) 1629, 2105, 2350
EcoT14I CCWWGG 5 cut(s) 117, 211, 919, 1233, 1309
EcoT38I GRGCYC 2 cut(s) 799, 2161
ErhI CCWWGG 5 cut(s) 117, 211, 919, 1233, 1309
Esp3I CGTCTC 1 cut(s) 442
FalI AAGNNNNNCTT 2 cut(s) 1575, 1607
FauI CCCGC 1 cut(s) 2661
FauNDI CATATG 2 cut(s) 273, 804
FbaI TGATCA 3 cut(s) 1155, 1458, 1936
FblI GTMKAC 3 cut(s) 375, 976, 1601
Fnu4HI GCNGC 6 cut(s) 289, 769, 1304, 1697, 2145, 2211
FriOI GRGCYC 2 cut(s) 799, 2161
Fsp4HI GCNGC 6 cut(s) 289, 769, 1304, 1697, 2145, 2211
FspBI CTAG 8 cut(s) 617, 735, 1101, 1646, 1985, 2264, 2294, 2444
FspI TGCGCA 1 cut(s) 1930
GlaI GCGC 3 cut(s) 367, 1524, 1930
GluI GCNGC 6 cut(s) 289, 769, 1304, 1697, 2145, 2211
GsaI CCCAGC 1 cut(s) 2091
HaeIII GGCC 7 cut(s) 48, 216, 462, 1278, 1590, 1974, 2105
HapII CCGG 3 cut(s) 34, 2176, 2680
HhaI GCGC 3 cut(s) 368, 1525, 1931
Hin6I GCGC 3 cut(s) 366, 1523, 1929
HinP1I GCGC 3 cut(s) 366, 1523, 1929
HincII GTYRAC 1 cut(s) 1627
HindII GTYRAC 1 cut(s) 1627
HindIII AAGCTT 4 cut(s) 168, 1191, 2298, 2531
HinfI GANTC 7 cut(s) 158, 239, 431, 889, 1093, 1814, 2243
HpaI GTTAAC 1 cut(s) 1627
HpaII CCGG 3 cut(s) 34, 2176, 2680
HphI GGTGA 1 cut(s) 1903
Hpy188I TCNGA 5 cut(s) 258, 791, 1014, 1396, 2281
Hpy188III TCNNGA 7 cut(s) 34, 986, 1497, 1750, 1776, 2186, 2458
HpyAV CCTTC 9 cut(s) 263, 559, 559, 786, 1580, 1608, 1882, 2092, 2270
HpyCH4III ACNGT 6 cut(s) 755, 1089, 1203, 1258, 2398, 2525
HpyCH4IV ACGT 3 cut(s) 826, 2388, 2430
HpyF10VI GCNNNNNNNGC 8 cut(s) 1177, 1409, 1676, 1856, 1865, 2134, 2207, 2560
HpyF3I CTNAG 8 cut(s) 182, 423, 483, 705, 893, 959, 2511, 2555
HpySE526I ACGT 3 cut(s) 826, 2388, 2430
HspAI GCGC 3 cut(s) 366, 1523, 1929
Kpn2I TCCGGA 1 cut(s) 33
KroI GCCGGC 1 cut(s) 2175
KroNI GCCGGC 1 cut(s) 2177
Ksp22I TGATCA 3 cut(s) 1155, 1458, 1936
KspAI GTTAAC 1 cut(s) 1627
LguI GCTCTTC 2 cut(s) 606, 2166
LmnI GCTCC 5 cut(s) 1163, 1300, 1311, 2156, 2714
Lsp1109I GCAGC 6 cut(s) 300, 755, 1315, 1683, 2131, 2222
LweI GCATC 9 cut(s) 37, 185, 292, 478, 1413, 2043, 2110, 2468, 2562
MaeI CTAG 8 cut(s) 617, 735, 1101, 1646, 1985, 2264, 2294, 2444
MaeII ACGT 3 cut(s) 826, 2388, 2430
MaeIII GTNAC 9 cut(s) 141, 383, 584, 1366, 1648, 1987, 2091, 2615, 2644
MboII GAAGA 9 cut(s) 310, 593, 1196, 1595, 2036, 2153, 2473, 2480, 2588
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 2 cut(s) 723, 2071
MhlI GDGCHC 3 cut(s) 694, 799, 2161
MlsI TGGCCA 3 cut(s) 216, 462, 1974
MluNI TGGCCA 3 cut(s) 216, 462, 1974
MlyI GAGTC 3 cut(s) 233, 425, 898
MmeI TCCRAC 4 cut(s) 329, 728, 1520, 2516
Mox20I TGGCCA 3 cut(s) 216, 462, 1974
MroI TCCGGA 1 cut(s) 33
MroNI GCCGGC 1 cut(s) 2175
MroXI GAANNNNTTC 1 cut(s) 407
MscI TGGCCA 3 cut(s) 216, 462, 1974
MseI TTAA 8 cut(s) 495, 591, 629, 1206, 1593, 1626, 2133, 2373
MslI CAYNNNNRTG 5 cut(s) 336, 815, 1005, 1284, 2523
Msp20I TGGCCA 3 cut(s) 216, 462, 1974
MspI CCGG 3 cut(s) 34, 2176, 2680
MspR9I CCNGG 3 cut(s) 1631, 2107, 2352
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 3 cut(s) 1363, 1466, 1852
MvaI CCWGG 3 cut(s) 1631, 2107, 2352
MvnI CGCG 3 cut(s) 368, 1525, 2187
MwoI GCNNNNNNNGC 8 cut(s) 1177, 1409, 1676, 1856, 1865, 2134, 2207, 2560
NaeI GCCGGC 1 cut(s) 2177
NcoI CCATGG 4 cut(s) 117, 211, 919, 1309
NdeI CATATG 2 cut(s) 273, 804
NgoMIV GCCGGC 1 cut(s) 2175
NlaIV GGNNCC 4 cut(s) 725, 917, 1029, 1165
NmuCI GTSAC 4 cut(s) 141, 383, 584, 2615
NruI TCGCGA 1 cut(s) 2187
NsbI TGCGCA 1 cut(s) 1930
NspI RCATGY 6 cut(s) 136, 676, 969, 1010, 1289, 2049
PaeI GCATGC 2 cut(s) 1010, 1289
PagI TCATGA 1 cut(s) 1749
PceI AGGCCT 2 cut(s) 48, 1590
PciI ACATGT 2 cut(s) 672, 2045
PciSI GCTCTTC 2 cut(s) 606, 2166
PcsI WCGNNNNNNNCGW 1 cut(s) 1018
PctI GAATGC 3 cut(s) 1363, 1466, 1852
PdiI GCCGGC 1 cut(s) 2177
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 4 cut(s) 158, 1093, 1814, 2243
PflFI GACNNNGTC 1 cut(s) 434
PfoI TCCNGGA 1 cut(s) 2350
PinAI ACCGGT 1 cut(s) 2679
PkrI GCNGC 6 cut(s) 290, 770, 1305, 1698, 2146, 2212
PleI GAGTC 3 cut(s) 233, 425, 897
PmaCI CACGTG 1 cut(s) 2389
PmlI CACGTG 1 cut(s) 2389
PpsI GAGTC 3 cut(s) 233, 425, 897
Ppu21I YACGTR 1 cut(s) 2389
PscI ACATGT 2 cut(s) 672, 2045
PshAI GACNNNNGTC 1 cut(s) 381
PsiI TTATAA 1 cut(s) 2193
Psp124BI GAGCTC 1 cut(s) 2161
Psp6I CCWGG 3 cut(s) 1629, 2105, 2350
PspCI CACGTG 1 cut(s) 2389
PspFI CCCAGC 1 cut(s) 2087
PspGI CCWGG 3 cut(s) 1629, 2105, 2350
PspN4I GGNNCC 4 cut(s) 725, 917, 1029, 1165
PstI CTGCAG 1 cut(s) 1701
PstNI CAGNNNCTG 1 cut(s) 1665
PsuI RGATCY 2 cut(s) 723, 2071
PsyI GACNNNGTC 1 cut(s) 434
RruI TCGCGA 1 cut(s) 2187
RsaI GTAC 4 cut(s) 677, 1200, 1246, 2385
RsaNI GTAC 4 cut(s) 676, 1199, 1245, 2384
RseI CAYNNNNRTG 5 cut(s) 336, 815, 1005, 1284, 2523
SacI GAGCTC 1 cut(s) 2161
SapI GCTCTTC 2 cut(s) 606, 2166
SaqAI TTAA 8 cut(s) 495, 591, 629, 1206, 1593, 1626, 2133, 2373
SatI GCNGC 6 cut(s) 289, 769, 1304, 1697, 2145, 2211
ScaI AGTACT 1 cut(s) 1200
SchI GAGTC 3 cut(s) 233, 425, 898
ScrFI CCNGG 3 cut(s) 1631, 2107, 2352
SduI GDGCHC 3 cut(s) 694, 799, 2161
SfaNI GCATC 9 cut(s) 37, 185, 292, 478, 1413, 2043, 2110, 2468, 2562
SfcI CTRYAG 4 cut(s) 228, 1350, 1697, 2397
SmiMI CAYNNNNRTG 5 cut(s) 336, 815, 1005, 1284, 2523
SmlI CTYRAG 2 cut(s) 1710, 2650
SmoI CTYRAG 2 cut(s) 1710, 2650
SpeI ACTAGT 1 cut(s) 1645
SphI GCATGC 2 cut(s) 1010, 1289
SseBI AGGCCT 2 cut(s) 48, 1590
SsiI CCGC 2 cut(s) 2201, 2668
SspI AATATT 2 cut(s) 82, 1683
SspMI CTAG 8 cut(s) 617, 735, 1101, 1646, 1985, 2264, 2294, 2444
SstI GAGCTC 1 cut(s) 2161
StuI AGGCCT 2 cut(s) 48, 1590
StyD4I CCNGG 3 cut(s) 1629, 2105, 2350
StyI CCWWGG 5 cut(s) 117, 211, 919, 1233, 1309
TaaI ACNGT 6 cut(s) 755, 1089, 1203, 1258, 2398, 2525
TaiI ACGT 3 cut(s) 829, 2391, 2433
TaqI TCGA 3 cut(s) 89, 188, 2599
TatI WGTACW 3 cut(s) 675, 1198, 1244
TfiI GAWTC 4 cut(s) 158, 1093, 1814, 2243
Tru1I TTAA 8 cut(s) 495, 591, 629, 1206, 1593, 1626, 2133, 2373
Tru9I TTAA 8 cut(s) 495, 591, 629, 1206, 1593, 1626, 2133, 2373
TscAI CASTG 6 cut(s) 699, 1165, 1666, 2401, 2519, 2530
TseFI GTSAC 4 cut(s) 141, 383, 584, 2615
TseI GCWGC 6 cut(s) 288, 768, 1303, 1696, 2144, 2210
Tsp45I GTSAC 4 cut(s) 141, 383, 584, 2615
TspGWI ACGGA 2 cut(s) 424, 1010
TspRI CASTG 6 cut(s) 699, 1165, 1666, 2401, 2519, 2530
Tth111I GACNNNGTC 1 cut(s) 434
VneI GTGCAC 1 cut(s) 690
XapI RAATTY 7 cut(s) 149, 631, 658, 1831, 2110, 2204, 2218
XceI RCATGY 6 cut(s) 136, 676, 969, 1010, 1289, 2049
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmiI GTMKAC 3 cut(s) 375, 976, 1601
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 8 cut(s) 617, 735, 1101, 1646, 1985, 2264, 2294, 2444
ZrmI AGTACT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.