Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0014296

Physical Location & Seq

Genomic Coordinates Forward (+)

81540997 .. 81543326

Gene Structure 2330 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00059550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGAAGGAGGGACTACACGGTTAGCTAAGCTATTGCAGGGTTGTATAGACAAGAAGGCCCAATTGGGCGGGAAGGTTATTCATGGGTTTATATTACGTAATAAAGGCTTGTACTTTGATACGTTTCTATCTAATAGACTCATTGAGTTGTACTCAAAGTGTGGTCATTTGGGGTATGCCCACCATGTGTTTGATAAAATGCCTAAGCCGGATGTGTACTCATGGAATGCTGTGTTGGGTTGTTATTGTAAAGCTGGGAAGTTGGGGGAGGCTGAAGAGTTATTTCTGAAAATGCCTGAGAGGAATGTTGTGTCGTGGAACACTCTGATTAGTACTTTGGTCCGAGATGGTCAGGAGGAAAAGGCTGTGGGTGTTTATGAGGCGATGGTTTCGGAGGGGGGTTGTTGGATGTGGAGAAGGGGAGGAAATGTCATGGGATTGTGGCGAAAAATTGGCCTCGAGGAGAATGTGTATGTGGGGAATGCTTTGCTGTGCATGTATGCTAAGTGTGGAGTTATGAGGGATGCAATGCAGGTTTTCGGTGACATGGCTGAGCCGAATGAGGTTACTTTTACGGCTATGATGGGGGGGTTGAAGCAGACGGACAGAGTCGCAGAGGCTTTGGAAATGTTCAGGCTGATGTGTAGGAAAGGGGTGCGTGTTGATTCTGTTTCGTTGTCGAGTATGTTGGGTGTTTGTGCCATAGGGTGTGGCGAGTCTGGTGTTCATGATTATAGTGATGGAATTCAGAGTAATGTGAACGGGCAACAAATACATGCCCTTACAGTTAAACTTGGATTTGAGGGAGATCTTCACTTGAACAATTCATTGCTTGATATGTATGCAAAGAGTGCGGACATGAAGAGTGCTGAGAAGGTTTTTGCTACTATACCTGAAGTTAGTGTTGTTTCTTGGAATGTCATGATAACTGGGTATGGCCTGATATTTCAGAGTCAGAAAGCTATAGAATATCTACGAAGAATGCAATCTTGGGGCTTTGAACCTGATGAGGTCACATATATCAATTTGCTTGCAGCATGTGTCAAGTCTGGTGATATAAAAAGTGGTCATCAAATGTTTGATAACATTTCATGCCCAAATGTGATTTCATGGAATGCAATACTCTCTGGCTATTTCCAGAATGAAGACCACATGGAAGTAATCAAGCTTTTCAGGGAAATGCAGTTTCGGGGTGTGCAACCTGATCGAACTACTTTGGCAATTGCTCTCAGCTCTAGTGCAGCAATGGGGATTCTGGAGGCTGGAGAAGAGATCCATGCTGCCTCACACAAGGCTGCCTTTGATACAGATATATATGTTGCAAGCGCACTTACTGGCATGTATTCAAAGTGTGGTAAGACAGAAATGGCAAAGCATATATTTCATAATATGCAAGAATTAGATGTTGTTTGTTGGAACTCTATGATAGCAGGTCTTTCACTAAATTCTCAAGATGAGGAAGCTTTCACTTACTTTAAGCAGATGAGGCAACATGAGATGCTTCCCACCCAATTCTCTTATGCTACGATACTGAGTTGCTGTGCAAAGCTTGCTTCTTCATTTCAAGGGAAACAGGTTCATGCTCAGATAACAAAAGATGGATATGTGAGCGATGTTTTTGTTGGGAGTGCTCTCATTGGTATGTATTGCAAATGTGGTGATGTAGATGGGGCCCGGAATTTTTTTGATATGATGTCAAGCAAAAGTGCGGTTACTTGGAATGAAATGATACATGGGTATGCACAGAATGGACGTGGAGATGAGGCTGTTCTTCTTTATTGGGACATGATTGCCTCAGCTGAGAGACCTGACGCCATTACTTTTGTTGCTGTTTTGACTGCTTGCAGCCATTCTGGATTGGTCGATGCTGGCATTGAAATATTTAATTCAATGGGGCACAAACATGGAGTGGAACCAGTTCTGGATCATTACACTTGCATTATTGATTCTCTTGGCCGTGCTGGTCGTTTCCAAGAAGCAGAAGTCTTACTAGATGAGATGCCATATAATGATGATCCAGTTATATGGGAGGTTTTGCTTAGTTCTTGTAGGGTTCATGCTAATGTGAGCTTAGCGAAAAGAGCAGCTGACGAACTCATCCGCTTGGACCCACATAATTCAGCTCCGTATGTGCTTTTGGCCAATATTTATTCTTCTTTAGGAAGATGGGACGAGGCAAGAGCTGTGAGAGATCTAATGAGTAACAAACAAGTCATCAAGGATCCAGGTTATAGCCGGATTGAGTACGAGAAGGGAATGCAATCCAGTATGGTGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

759

Amino Acids

84.19

Weight (kDa)

5.64

Isoelectric Point (pI)

39.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_1	PF12854	68 - 98	1.1e-07	PPR repeat
PPR_2	PF13041	70 - 103	1.6e-07	PPR repeat family
PPR	PF01535	73 - 103	3.8e-08	PPR repeat
PPR_2	PF13041	186 - 233	1.7e-08	PPR repeat family
PPR_2	PF13041	301 - 349	1.9e-09	PPR repeat family
PPR_2	PF13041	366 - 410	4.8e-11	PPR repeat family
PPR	PF01535	369 - 399	8.2e-06	PPR repeat
PPR_2	PF13041	468 - 516	2.7e-08	PPR repeat family
PPR_2	PF13041	569 - 616	4.5e-09	PPR repeat family
PPR	PF01535	571 - 597	7.9e-06	PPR repeat
PPR_1	PF12854	636 - 667	2.3e-06	PPR repeat
E_motif	PF20431	686 - 748	3.9e-15	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20770
fragaria_vesca	FvH4_3g11160
malus_domestica	MD10G1243200.v1.1
prunus_persica	Prupe.4G100900_v2.0.a1 Prupe.4G100900_v2.0.a1
pyrus_communis	pycom10g20340
rosa_chinensis	RchiOBHm_Chr5g0018061
rosa_laevigata	RLG00000032337
rosa_multiflora	Rmu_co8213086.1_g000001 Rmu_sc0010280.1_g000017 Rmu_sc0026609.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059550
rosa_rugosa	Rorug05G0040100
rosa_samantha	Rh5BG131500 Rh5CG143500 Rh5DG133200
rosa_wichuraiana	Rw5G011580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	458
Acc36I	ACCTGC	2 cut(s)	523, 1421
AciI	CCGC	4 cut(s)	69, 854, 1709, 2101
AclWI	GGATC	5 cut(s)	1267, 1932, 2009, 2216, 2229
AcoI	YGGCCR	2 cut(s)	1954, 2139
AcsI	RAATTY	3 cut(s)	744, 1444, 1678
AcuI	CTGAAG	2 cut(s)	294, 915
AcyI	GRCGYC	1 cut(s)	1812
AdeI	CACNNNGTG	1 cut(s)	187
AfaI	GTAC	5 cut(s)	113, 152, 218, 334, 2246
AgsI	TTSAA	7 cut(s)	595, 820, 1001, 1347, 1565, 1877, 1890
AjiI	CACGTC	1 cut(s)	1754
AjnI	CCWGG	1 cut(s)	2224
AjuI	GAANNNNNNNTTGG	8 cut(s)	47, 79, 218, 250, 973, 1005, 1090, 1122
Alw21I	GWGCWC	1 cut(s)	1633
Alw26I	GTCTC	1 cut(s)	1798
AlwI	GGATC	5 cut(s)	1267, 1932, 2009, 2216, 2229
Ama87I	CYCGRG	1 cut(s)	458
AoxI	GGCC	6 cut(s)	57, 454, 937, 1671, 1954, 2139
ApaI	GGGCCC	1 cut(s)	1675
ApeKI	GCWGC	6 cut(s)	1034, 1241, 1280, 1295, 1845, 2084
ApoI	RAATTY	3 cut(s)	744, 1444, 1678
Asp700I	GAANNNNTTC	1 cut(s)	1917
AspLEI	GCGC	1 cut(s)	1328
AspS9I	GGNCC	5 cut(s)	58, 340, 1671, 1672, 2107
AsuC2I	CCSGG	1 cut(s)	1675
AsuHPI	GGTGA	3 cut(s)	554, 1064, 1670
AvaI	CYCGRG	1 cut(s)	458
AvaII	GGWCC	2 cut(s)	340, 2107
BaeGI	GKGCMC	2 cut(s)	1675, 1899
BaeI	ACNNNNGTAYC	2 cut(s)	1721, 1754
BalI	TGGCCA	1 cut(s)	2141
BamHI	GGATCC	1 cut(s)	2221
BanII	GRGCYC	1 cut(s)	1675
BbsI	GAAGAC	1 cut(s)	1152
Bbv12I	GWGCWC	1 cut(s)	1633
BbvCI	CCTCAGC	1 cut(s)	1795
BbvI	GCAGC	6 cut(s)	1046, 1253, 1267, 1282, 1857, 2096
BccI	CCATC	7 cut(s)	341, 379, 577, 734, 1592, 1661, 2160
BceAI	ACGGC	2 cut(s)	591, 1941
BcgI	CGANNNNNNTGC	2 cut(s)	1187, 1221
BciT130I	CCWGG	1 cut(s)	2226
BcnI	CCSGG	1 cut(s)	1675
BcoDI	GTCTC	1 cut(s)	1798
BfaI	CTAG	2 cut(s)	1236, 1991
BfmI	CTRYAG	1 cut(s)	963
BfuAI	ACCTGC	2 cut(s)	523, 1421
BglII	AGATCT	2 cut(s)	808, 2191
BisI	GCNGC	6 cut(s)	1035, 1242, 1281, 1296, 1846, 2085
BlpI	GCTNAGC	3 cut(s)	27, 552, 2071
BlsI	GCNGC	6 cut(s)	1036, 1243, 1282, 1297, 1847, 2086
BmcAI	AGTACT	1 cut(s)	334
Bme1390I	CCNGG	2 cut(s)	1675, 2226
Bme18I	GGWCC	2 cut(s)	340, 2107
BmeT110I	CYCGRG	1 cut(s)	458
BmgBI	CACGTC	1 cut(s)	1754
BmgT120I	GGNCC	5 cut(s)	58, 340, 1671, 1672, 2107
BmiI	GGNNCC	5 cut(s)	1672, 1673, 1914, 2109, 2223
BmrFI	CCNGG	2 cut(s)	1675, 2226
BmrI	ACTGGG	1 cut(s)	939
BmsI	GCATC	4 cut(s)	514, 1488, 1855, 1989
BmuI	ACTGGG	1 cut(s)	939
BpiI	GAAGAC	1 cut(s)	1152
BplI	GAGNNNNNCTC	2 cut(s)	137, 169
BpmI	CTGGAG	2 cut(s)	1277, 1284
Bpu10I	CCTNAGC	2 cut(s)	204, 1795
Bpu1102I	GCTNAGC	3 cut(s)	27, 552, 2071
BpuEI	CTTGAG	1 cut(s)	1434
BpuMI	CCSGG	1 cut(s)	1675
BsaAI	YACGTR	1 cut(s)	98
BsaHI	GRCGYC	1 cut(s)	1812
BsaI	GGTCTC	1 cut(s)	1798
BsaXI	ACNNNNNCTCC	4 cut(s)	414, 444, 455, 485
Bse1I	ACTGG	5 cut(s)	934, 1339, 1916, 2018, 2265
Bse3DI	GCAATG	3 cut(s)	534, 827, 1251
BseBI	CCWGG	1 cut(s)	2226
BseGI	GGATG	4 cut(s)	217, 414, 529, 2097
BseMI	GCAATG	3 cut(s)	534, 827, 1251
BseMII	CTCAG	8 cut(s)	288, 543, 861, 1243, 1523, 1598, 1791, 1809
BseNI	ACTGG	5 cut(s)	934, 1339, 1916, 2018, 2265
BseRI	GAGGAG	1 cut(s)	476
BseSI	GKGCMC	2 cut(s)	1675, 1899
BseXI	GCAGC	6 cut(s)	1046, 1253, 1267, 1282, 1857, 2096
BseYI	CCCAGC	1 cut(s)	254
BsgI	GTGCAG	1 cut(s)	1260
BshFI	GGCC	6 cut(s)	59, 456, 939, 1673, 1956, 2141
BsiHKAI	GWGCWC	1 cut(s)	1633
BsiHKCI	CYCGRG	1 cut(s)	458
BsiSI	CCGG	3 cut(s)	209, 1675, 2236
BslFI	GGGAC	3 cut(s)	25, 1796, 2183
BsmAI	GTCTC	1 cut(s)	1798
BsmFI	GGGAC	3 cut(s)	25, 1796, 2183
BsmI	GAATGC	5 cut(s)	232, 487, 987, 1120, 2262
BsnI	GGCC	6 cut(s)	59, 456, 939, 1673, 1956, 2141
Bso31I	GGTCTC	1 cut(s)	1798
BsoBI	CYCGRG	1 cut(s)	458
Bsp120I	GGGCCC	1 cut(s)	1671
Bsp1286I	GDGCHC	3 cut(s)	1633, 1675, 1899
Bsp143I	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
Bsp1720I	GCTNAGC	3 cut(s)	27, 552, 2071
BspACI	CCGC	4 cut(s)	69, 854, 1709, 2101
BspANI	GGCC	6 cut(s)	59, 456, 939, 1673, 1956, 2141
BspCNI	CTCAG	8 cut(s)	289, 544, 862, 1242, 1524, 1597, 1792, 1808
BspHI	TCATGA	2 cut(s)	727, 921
BspLI	GGNNCC	5 cut(s)	1672, 1673, 1914, 2109, 2223
BspMI	ACCTGC	2 cut(s)	523, 1421
BspPI	GGATC	5 cut(s)	1267, 1932, 2009, 2216, 2229
BspTNI	GGTCTC	1 cut(s)	1798
BsrDI	GCAATG	3 cut(s)	534, 827, 1251
BsrI	ACTGG	5 cut(s)	934, 1339, 1916, 2018, 2265
BssMI	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
BssNI	GRCGYC	1 cut(s)	1812
Bst2UI	CCWGG	1 cut(s)	2226
Bst4CI	ACNGT	2 cut(s)	21, 787
Bst6I	CTCTTC	3 cut(s)	270, 857, 1263
BstACI	GRCGYC	1 cut(s)	1812
BstAPI	GCANNNNNTGC	2 cut(s)	851, 1550
BstBAI	YACGTR	1 cut(s)	98
BstC8I	GCNNGC	5 cut(s)	1032, 1324, 1551, 1843, 1870
BstF5I	GGATG	4 cut(s)	217, 414, 529, 2097
BstHHI	GCGC	1 cut(s)	1328
BstKTI	GATC	7 cut(s)	811, 1207, 1275, 1927, 2017, 2194, 2224
BstMAI	GTCTC	1 cut(s)	1798
BstMBI	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
BstMWI	GCNNNNNNNGC	4 cut(s)	851, 1486, 1550, 2081
BstNI	CCWGG	1 cut(s)	2226
BstNSI	RCATGY	4 cut(s)	499, 779, 1041, 1342
BstSCI	CCNGG	2 cut(s)	1673, 2224
BstSFI	CTRYAG	1 cut(s)	963
BstSLI	GKGCMC	2 cut(s)	1675, 1899
BstSNI	TACGTA	1 cut(s)	98
BstV1I	GCAGC	6 cut(s)	1046, 1253, 1267, 1282, 1857, 2096
BstV2I	GAAGAC	1 cut(s)	1152
BstX2I	RGATCY	4 cut(s)	808, 1272, 2191, 2221
BstXI	CCANNNNNNTGG	2 cut(s)	2025, 2272
BstYI	RGATCY	4 cut(s)	808, 1272, 2191, 2221
BsuRI	GGCC	6 cut(s)	59, 456, 939, 1673, 1956, 2141
BtgZI	GCGATG	2 cut(s)	398, 1626
BtrI	CACGTC	1 cut(s)	1754
BtsCI	GGATG	4 cut(s)	217, 414, 529, 2097
BveI	ACCTGC	2 cut(s)	523, 1421
Cac8I	GCNNGC	5 cut(s)	1032, 1324, 1551, 1843, 1870
CciI	TCATGA	2 cut(s)	727, 921
CfoI	GCGC	1 cut(s)	1328
Cfr13I	GGNCC	5 cut(s)	58, 340, 1671, 1672, 2107
CseI	GACGC	1 cut(s)	1820
Csp6I	GTAC	5 cut(s)	112, 151, 217, 333, 2245
CviQI	GTAC	5 cut(s)	112, 151, 217, 333, 2245
DpnI	GATC	7 cut(s)	810, 1206, 1274, 1926, 2016, 2193, 2223
DpnII	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
DraIII	CACNNNGTG	1 cut(s)	187
EaeI	YGGCCR	2 cut(s)	1954, 2139
Eam1104I	CTCTTC	3 cut(s)	270, 857, 1263
EarI	CTCTTC	3 cut(s)	270, 857, 1263
Eco105I	TACGTA	1 cut(s)	98
Eco24I	GRGCYC	1 cut(s)	1675
Eco31I	GGTCTC	1 cut(s)	1798
Eco47I	GGWCC	2 cut(s)	340, 2107
Eco57I	CTGAAG	2 cut(s)	294, 915
Eco88I	CYCGRG	1 cut(s)	458
EcoO109I	RGGNCCY	1 cut(s)	1671
EcoRI	GAATTC	1 cut(s)	744
EcoRII	CCWGG	1 cut(s)	2224
EcoT38I	GRGCYC	1 cut(s)	1675
FalI	AAGNNNNNCTT	2 cut(s)	1283, 1315
FaqI	GGGAC	3 cut(s)	25, 1796, 2183
FauI	CCCGC	1 cut(s)	62
Fnu4HI	GCNGC	6 cut(s)	1035, 1242, 1281, 1296, 1846, 2085
FokI	GGATG	4 cut(s)	224, 421, 536, 2084
FriOI	GRGCYC	1 cut(s)	1675
Fsp4HI	GCNGC	6 cut(s)	1035, 1242, 1281, 1296, 1846, 2085
FspBI	CTAG	2 cut(s)	1236, 1991
GlaI	GCGC	1 cut(s)	1327
GluI	GCNGC	6 cut(s)	1035, 1242, 1281, 1296, 1846, 2085
GsaI	CCCAGC	1 cut(s)	258
GsuI	CTGGAG	2 cut(s)	1277, 1284
HaeIII	GGCC	6 cut(s)	59, 456, 939, 1673, 1956, 2141
HapII	CCGG	3 cut(s)	209, 1675, 2236
HgaI	GACGC	1 cut(s)	1820
HhaI	GCGC	1 cut(s)	1328
Hin1I	GRCGYC	1 cut(s)	1812
Hin6I	GCGC	1 cut(s)	1326
HinP1I	GCGC	1 cut(s)	1326
HindIII	AAGCTT	3 cut(s)	1166, 1461, 1547
HinfI	GANTC	7 cut(s)	138, 609, 665, 716, 952, 1252, 1946
HpaII	CCGG	3 cut(s)	209, 1675, 2236
HphI	GGTGA	3 cut(s)	554, 1064, 1670
Hpy166II	GTNNAC	2 cut(s)	217, 760
Hpy188I	TCNGA	8 cut(s)	288, 327, 344, 394, 750, 951, 957, 1587
Hpy188III	TCNNGA	8 cut(s)	353, 728, 922, 1138, 1256, 1451, 1854, 1922
Hpy8I	GTNNAC	2 cut(s)	217, 760
HpyAV	CCTTC	5 cut(s)	49, 67, 411, 868, 2245
HpyCH4III	ACNGT	2 cut(s)	21, 787
HpyCH4IV	ACGT	3 cut(s)	97, 122, 1753
HpyF10VI	GCNNNNNNNGC	4 cut(s)	851, 1486, 1550, 2081
HpySE526I	ACGT	3 cut(s)	97, 122, 1753
Hsp92I	GRCGYC	1 cut(s)	1812
HspAI	GCGC	1 cut(s)	1326
Kzo9I	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
LmnI	GCTCC	1 cut(s)	2128
Lsp1109I	GCAGC	6 cut(s)	1046, 1253, 1267, 1282, 1857, 2096
LweI	GCATC	4 cut(s)	514, 1488, 1855, 1989
MaeI	CTAG	2 cut(s)	1236, 1991
MaeII	ACGT	3 cut(s)	97, 122, 1753
MaeIII	GTNAC	5 cut(s)	542, 565, 1012, 1711, 2201
MalI	GATC	7 cut(s)	810, 1206, 1274, 1926, 2016, 2193, 2223
MboI	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
MfeI	CAATTG	2 cut(s)	62, 1221
MflI	RGATCY	4 cut(s)	808, 1272, 2191, 2221
MhlI	GDGCHC	3 cut(s)	1633, 1675, 1899
MlsI	TGGCCA	1 cut(s)	2141
MluNI	TGGCCA	1 cut(s)	2141
MlyI	GAGTC	4 cut(s)	132, 618, 725, 961
MmeI	TCCRAC	2 cut(s)	386, 1394
Mox20I	TGGCCA	1 cut(s)	2141
MroXI	GAANNNNTTC	1 cut(s)	1917
MscI	TGGCCA	1 cut(s)	2141
MseI	TTAA	3 cut(s)	789, 1476, 1884
MslI	CAYNNNNRTG	4 cut(s)	1640, 1737, 1902, 2061
Msp20I	TGGCCA	1 cut(s)	2141
MspA1I	CMGCKG	2 cut(s)	1799, 2087
MspI	CCGG	3 cut(s)	209, 1675, 2236
MspR9I	CCNGG	2 cut(s)	1675, 2226
MunI	CAATTG	2 cut(s)	62, 1221
Mva1269I	GAATGC	5 cut(s)	232, 487, 987, 1120, 2262
MvaI	CCWGG	1 cut(s)	2226
MwoI	GCNNNNNNNGC	4 cut(s)	851, 1486, 1550, 2081
NciI	CCSGG	1 cut(s)	1675
NdeII	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
NlaIV	GGNNCC	5 cut(s)	1672, 1673, 1914, 2109, 2223
NmuCI	GTSAC	2 cut(s)	542, 1012
NspI	RCATGY	4 cut(s)	499, 779, 1041, 1342
PaeR7I	CTCGAG	1 cut(s)	458
PagI	TCATGA	2 cut(s)	727, 921
PctI	GAATGC	5 cut(s)	232, 487, 987, 1120, 2262
PdmI	GAANNNNTTC	1 cut(s)	1917
PfeI	GAWTC	3 cut(s)	665, 1252, 1946
PflFI	GACNNNGTC	1 cut(s)	608
PkrI	GCNGC	6 cut(s)	1036, 1243, 1282, 1297, 1847, 2086
PleI	GAGTC	4 cut(s)	132, 617, 724, 960
PpsI	GAGTC	4 cut(s)	132, 617, 724, 960
Ppu21I	YACGTR	1 cut(s)	98
Psp6I	CCWGG	1 cut(s)	2224
PspFI	CCCAGC	1 cut(s)	254
PspGI	CCWGG	1 cut(s)	2224
PspN4I	GGNNCC	5 cut(s)	1672, 1673, 1914, 2109, 2223
PspOMI	GGGCCC	1 cut(s)	1671
PspPI	GGNCC	5 cut(s)	58, 340, 1671, 1672, 2107
PspXI	VCTCGAGB	1 cut(s)	458
PsuI	RGATCY	4 cut(s)	808, 1272, 2191, 2221
PsyI	GACNNNGTC	1 cut(s)	608
PvuII	CAGCTG	2 cut(s)	1799, 2087
RsaI	GTAC	5 cut(s)	113, 152, 218, 334, 2246
RsaNI	GTAC	5 cut(s)	112, 151, 217, 333, 2245
RseI	CAYNNNNRTG	4 cut(s)	1640, 1737, 1902, 2061
SaqAI	TTAA	3 cut(s)	789, 1476, 1884
SatI	GCNGC	6 cut(s)	1035, 1242, 1281, 1296, 1846, 2085
Sau3AI	GATC	7 cut(s)	808, 1204, 1272, 1924, 2014, 2191, 2221
Sau96I	GGNCC	5 cut(s)	58, 340, 1671, 1672, 2107
ScaI	AGTACT	1 cut(s)	334
SchI	GAGTC	4 cut(s)	132, 618, 725, 961
ScrFI	CCNGG	2 cut(s)	1675, 2226
SduI	GDGCHC	3 cut(s)	1633, 1675, 1899
SfaNI	GCATC	4 cut(s)	514, 1488, 1855, 1989
SfcI	CTRYAG	1 cut(s)	963
Sfr274I	CTCGAG	1 cut(s)	458
SinI	GGWCC	2 cut(s)	340, 2107
SlaI	CTCGAG	1 cut(s)	458
SmiMI	CAYNNNNRTG	4 cut(s)	1640, 1737, 1902, 2061
SmlI	CTYRAG	2 cut(s)	458, 1449
SmoI	CTYRAG	2 cut(s)	458, 1449
SnaBI	TACGTA	1 cut(s)	98
SsiI	CCGC	4 cut(s)	69, 854, 1709, 2101
SspI	AATATT	2 cut(s)	1881, 2146
SspMI	CTAG	2 cut(s)	1236, 1991
StyD4I	CCNGG	2 cut(s)	1673, 2224
TaaI	ACNGT	2 cut(s)	21, 787
TaiI	ACGT	3 cut(s)	100, 125, 1756
TaqI	TCGA	4 cut(s)	459, 680, 1207, 1863
TatI	WGTACW	4 cut(s)	111, 150, 216, 332
TfiI	GAWTC	3 cut(s)	665, 1252, 1946
Tru1I	TTAA	3 cut(s)	789, 1476, 1884
Tru9I	TTAA	3 cut(s)	789, 1476, 1884
TseFI	GTSAC	2 cut(s)	542, 1012
TseI	GCWGC	6 cut(s)	1034, 1241, 1280, 1295, 1845, 2084
Tsp45I	GTSAC	2 cut(s)	542, 1012
TspGWI	ACGGA	2 cut(s)	617, 2115
Tth111I	GACNNNGTC	1 cut(s)	608
VpaK11BI	GGWCC	2 cut(s)	340, 2107
XapI	RAATTY	3 cut(s)	744, 1444, 1678
XceI	RCATGY	4 cut(s)	499, 779, 1041, 1342
XhoI	CTCGAG	1 cut(s)	458
XmnI	GAANNNNTTC	1 cut(s)	1917
XspI	CTAG	2 cut(s)	1236, 1991
ZrmI	AGTACT	1 cut(s)	334

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.