Rroxscaffold_2G00091720

Clathrin coat assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014561
Physical Location & Seq
Forward (+)
13252200 .. 13255598
3399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091720.1

Sequence Viewer

Length: 2586 bp
ATGCCAAGCAAGTTAAAGAAGGCAATCGGGGCAGTGAAAGACCAGACGAGCATAAGCCTAGCCAAGGTCTCCAATACCTCCAACCTCGCCAGCCTCGAAGTTACCATCCTCAAGGCCACCTCCCACGACGTCGTTCCGATCGACGACAAGTACGTCCGTGAGATCCTCTCCCTCATCTCTGCCTCCAAATCCAACGCCGCCACCTGCGCCCAAGCCATCGCCAAACGCATCGGCAAGACCCGCAACTGGATCGTCGCCATCAAGTCCCTCATGCTCGTCCTCCGAATCTTCCAGGACGGCGACCCCTATTTCCCCATTGAAGTCCTCCACGCCATGAAACGTGGCGCCAAGATCCTTAACCTCTCCAGTTTTCGTGATGACTCGACCTCATGTCCCTGGGACTACACCGCCTTCGTCCGCAACTTCGCTCTCTACCTTGAAGAAAGGCTCGACTGCTTCCTCACCGGAAAGCTCCAGCGGCGTTTCACCTACCAGAGAGATCAGGATTGCATTATCATCAACAACACCCGTAACCGCCGACGAGGCAATAATAATGAACCGGTGGTTCGGGACATGAAGCCGGCGATGCTGCTGGACCGGATTCAATTCTGGCAGAAACTGCTGGATCGAGCCATTGCCTTAATCCCCTCCGGTGCCGCCAAAACGAACCGTTTAATCCTCATTTCCCTCTACGCAATCGTCCAGGAGACCTACGATCTATACAGAGACATTTCGGACGGGCTGGCTTTGCTTTTAGATAGCTTTTTTCATCTCCAATACCATTCATGTCTAAGTGCTTTTCATGCATGCGTAAAAGCCTCGAAGCAATTCGAAGAGCTTTCTACTTTTTATGGAACTTGCAAGGCGTTAGGTGTTGGGAGGACCTCTGAGTATCCAAGCGTGCAGAAAATTTCTGAGGAGCTGATCGAGACATTGCAGGAGTTTGTGAAAGACCAAGCTTCATTTCCAGGTCGATCGCCGCCGCAGTATATGAATCAGAATGTTAAAGATAAAGAGGCGGCATCCAGTCCGGACCAATTTGAGTTTGCTAATTCGGAGCCGTTCGAGGGTGCATCGGAGAGAGGCTCAGCTTTTGGTTCGGCTTGTACGTCGTTATCGGATTTGATGAGTGTTGGCGGTATAGAAAATGGGGCTAGCCCATCGTGGTCGGCCGAGCAAGATTTTCTTACCTCCGATGAGCAATTGTCCGAGAAGACCCTATCTCCGTTTCAGTCACCGTTACCGGAATTCATGGTTGGTAGTAATGATAAAGAATCGAGCCAATCTTTGCCGCAGATTGATCTTTTTGAGGTCTTTCCAAAGCAACAAACAGATCAGGTTCAACAAGCTAATCAAGAAGGTGAAAAACCTGCATGGGAAGTTGTTCTTTTTGAGTCTGCAAATCAAAGTGGTGCAATGGAAACATCATCGATTGCTTCGCCTCCTCCCAACCTGTTTGCTTCTCCTTCTCCTAACCTGTCTCCTAATCTGTTGGAATCGTCGTCCAGCCCGGACTTGGACTATTTCTTTCAGAATTCTTTGACGAGCAATGCATTACACCAAAACAATCCATTTCTTCAAGACATTGTACAAGTTGAGGGCTTTGCAATTCCCACTTTCAATGAGACAAGTAGTAACGCTGGCTTAGAAAATTTGGACGATTTGTTTTCGTTCCCTTCTACATCTCAGCCATCGGCGCCACCAACATTTGGTGTGCGGAGTTCCAATGGGAGCACAGTGGCTCCAACATTTGTGGCTGACAGTTCAGAACAAAGCACAATGTTACCAACATTTTCTTCACATAATTCAAATGGGAACACAATGGCACCAACATTCTTGACCAATAATCCAAAACAAGGCACAATGTCTCCCACATTTTCTGCACAGAACTCAAATGGGAGAAATGTAGCGCCAACGTACTTGGCCCAGAGTACAAAACAAAGCACGATGTCACCAAGGTTTTCTTCACAGAACTCAAATGAGAGGGCGGTGGCTCCAACCTTTTGTACGGCGGATACAATAGATAGCAAAGTGGTACCAACAAATCCAAATGAGACTGAAATTGCACCAACATTTTCAGCTCAGAGTGGGGAAGGAAGCTCAATGTCACCACCTCTTTGTGCGCAGAATATAAATAATAGCATAACAGCACCAACATTTCAAGCAGATTTTCCGTGGAATAGGGCTTTGGCACTACCATCTTGTGCAGAAAATTTGAAAGAGACTGAAATCGCACCATCATTTTCCGCTCAAAGCCCTGAAGGAAGCTCATTTTCACCATCCTTTTGTGCGCAGAATACAAATAATAGCATAACAGCACCAACATTTCATGCAGAATTTCCAAAGGATAAGACTTTGGCCCTTTCAATTTATGCAGAAAATTCAAGAGAGAGCAGAATGTCGCAAACGTTTGGGGAGCTGACTCCTAATAATGGGAGTGTAGTAGCACCAACCTTCAGTTCAGAGGAACCTAATGAGATGTCATGGGACCAAAAAGGCCCAGTTAACTCGACCGTCATTATAACCTTGGACTGTTGGCTGGAAAGTGGCAGAAATCAGCACTGGCTGTTGGTTCATGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

861

Amino Acids

94.44

Weight (kDa)

4.93

Isoelectric Point (pI)

59.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 32 - 318 1.8e-78 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014561)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05020
fragaria_vesca FvH4_6g42130 FvH4_6g42160 FvH4_6g42170
malus_domestica MD09G1114300.v1.1 MD17G1106000.v1.1
prunus_persica Prupe.3G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158041
rosa_laevigata RLG00000021052
rosa_multiflora Rmu_sc0000419.1_g000017
rosa_roxburghii Rroxscaffold_2G00091720
rosa_rugosa Rorug02G0465400
rosa_samantha Rh2AG531400 Rh2BG545900 Rh2CG515700 Rh2DG554000
rosa_wichuraiana Rw2G043950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2522
AarI CACCTGC 1 cut(s) 212
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 132
Acc16I TGCGCA 2 cut(s) 2124, 2292
Acc36I ACCTGC 2 cut(s) 212, 1378
Acc65I GGTACC 1 cut(s) 2035
AccB1I GGYRCC 5 cut(s) 344, 653, 1696, 1825, 2035
AccB7I CCANNNNNTGG 1 cut(s) 1709
AccBSI CCGCTC 1 cut(s) 2249
AccIII TCCGGA 1 cut(s) 1030
AclI AACGTT 1 cut(s) 2408
AclWI GGATC 4 cut(s) 157, 257, 346, 633
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 7 cut(s) 909, 1247, 1534, 1651, 2212, 2336, 2380
AcuI CTGAAG 2 cut(s) 2280, 2440
AcyI GRCGYC 3 cut(s) 129, 345, 1697
AfaI GTAC 7 cut(s) 152, 1108, 1590, 1919, 1933, 2008, 2037
AfiI CCNNNNNNNGG 7 cut(s) 64, 246, 1067, 1516, 1709, 1856, 2432
AgeI ACCGGT 1 cut(s) 559
AhdI GACNNNNNGTC 1 cut(s) 390
AjnI CCWGG 4 cut(s) 291, 395, 702, 967
Alw21I GWGCWC 1 cut(s) 1736
Alw26I GTCTC 9 cut(s) 73, 701, 720, 923, 1485, 1619, 1872, 2048, 2216
AlwI GGATC 4 cut(s) 157, 257, 346, 633
AlwNI CAGNNNCTG 1 cut(s) 619
Aor13HI TCCGGA 1 cut(s) 1030
AoxI GGCC 5 cut(s) 114, 1170, 1923, 2358, 2497
ApeKI GCWGC 1 cut(s) 589
ApoI RAATTY 7 cut(s) 909, 1247, 1534, 1651, 2212, 2336, 2380
AsiGI ACCGGT 1 cut(s) 559
Asp700I GAANNNNTTC 2 cut(s) 827, 1383
Asp718I GGTACC 1 cut(s) 2035
AspLEI GCGC 6 cut(s) 209, 347, 1699, 1912, 2125, 2293
AspS9I GGNCC 7 cut(s) 595, 882, 1033, 1924, 2359, 2488, 2498
AsuC2I CCSGG 1 cut(s) 1511
AsuHPI GGTGA 7 cut(s) 454, 478, 1227, 1373, 1944, 2100, 2268
AsuII TTCGAA 1 cut(s) 831
AsuNHI GCTAGC 1 cut(s) 1154
AvaII GGWCC 4 cut(s) 595, 882, 1033, 2488
BanI GGYRCC 5 cut(s) 344, 653, 1696, 1825, 2035
BbsI GAAGAC 1 cut(s) 1220
Bbv12I GWGCWC 1 cut(s) 1736
BbvI GCAGC 1 cut(s) 576
BccI CCATC 8 cut(s) 113, 224, 266, 1168, 1699, 2206, 2245, 2287
BceAI ACGGC 3 cut(s) 313, 1045, 2025
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 4 cut(s) 293, 397, 704, 969
BciVI GTATCC 2 cut(s) 903, 2008
BcnI CCSGG 1 cut(s) 1511
BcoDI GTCTC 9 cut(s) 73, 701, 720, 923, 1485, 1619, 1872, 2048, 2216
BfaI CTAG 2 cut(s) 59, 1155
BfoI RGCGCY 3 cut(s) 348, 1700, 1913
BfuAI ACCTGC 2 cut(s) 212, 1378
BfuI GTATCC 2 cut(s) 903, 2008
BglI GCCNNNNNGGC 1 cut(s) 543
BisI GCNGC 8 cut(s) 198, 479, 590, 657, 980, 983, 1020, 1292
BlpI GCTNAGC 1 cut(s) 1087
BlsI GCNGC 8 cut(s) 199, 480, 591, 658, 981, 984, 1021, 1293
Bme1390I CCNGG 5 cut(s) 293, 397, 704, 969, 1511
Bme18I GGWCC 4 cut(s) 595, 882, 1033, 2488
BmeRI GACNNNNNGTC 1 cut(s) 390
BmgT120I GGNCC 7 cut(s) 595, 882, 1033, 1924, 2359, 2488, 2498
BmrFI CCNGG 5 cut(s) 293, 397, 704, 969, 1511
BmrI ACTGGG 1 cut(s) 2495
BmsI GCATC 4 cut(s) 237, 576, 1031, 1082
BmtI GCTAGC 1 cut(s) 1158
BmuI ACTGGG 1 cut(s) 2495
BpiI GAAGAC 1 cut(s) 1220
BplI GAGNNNNNCTC 4 cut(s) 152, 184, 1070, 1102
BpmI CTGGAG 2 cut(s) 349, 458
Bpu1102I GCTNAGC 1 cut(s) 1087
Bpu14I TTCGAA 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 1511
Bsa29I ATCGAT 1 cut(s) 1430
BsaHI GRCGYC 3 cut(s) 129, 345, 1697
BsaI GGTCTC 2 cut(s) 73, 701
BsaJI CCNNGG 6 cut(s) 63, 395, 396, 1955, 2174, 2526
BsaWI WCCGGW 6 cut(s) 464, 559, 597, 650, 1030, 1243
BsaXI ACNNNNNCTCC 6 cut(s) 1207, 1237, 1726, 1756, 1852, 1882
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 246, 1067, 1516, 1709, 1856, 2432
Bse118I RCCGGY 2 cut(s) 559, 580
Bse1I ACTGG 5 cut(s) 251, 366, 1026, 2501, 2567
Bse3DI GCAATG 4 cut(s) 633, 932, 1422, 1555
BseAI TCCGGA 1 cut(s) 1030
BseBI CCWGG 4 cut(s) 293, 397, 704, 969
BseCI ATCGAT 1 cut(s) 1430
BseDI CCNNGG 6 cut(s) 63, 395, 396, 1955, 2174, 2526
BseGI GGATG 3 cut(s) 105, 1022, 2279
BseLI CCNNNNNNNGG 7 cut(s) 64, 246, 1067, 1516, 1709, 1856, 2432
BseMI GCAATG 4 cut(s) 633, 932, 1422, 1555
BseMII CTCAG 5 cut(s) 879, 906, 1101, 1700, 2096
BseNI ACTGG 5 cut(s) 251, 366, 1026, 2501, 2567
BseRI GAGGAG 2 cut(s) 932, 1434
BseX3I CGGCCG 1 cut(s) 1170
BseXI GCAGC 1 cut(s) 576
BsgI GTGCAG 3 cut(s) 923, 1866, 2226
Bsh1285I CGRYCG 4 cut(s) 141, 977, 1173, 2514
BshFI GGCC 5 cut(s) 116, 1172, 1925, 2360, 2499
BshNI GGYRCC 5 cut(s) 344, 653, 1696, 1825, 2035
BshTI ACCGGT 1 cut(s) 559
BshVI ATCGAT 1 cut(s) 1430
BsiEI CGRYCG 4 cut(s) 141, 977, 1173, 2514
BsiHKAI GWGCWC 1 cut(s) 1736
BsiSI CCGG 8 cut(s) 465, 560, 581, 598, 651, 1031, 1244, 1511
BslFI GGGAC 5 cut(s) 250, 378, 413, 584, 2501
BslI CCNNNNNNNGG 7 cut(s) 64, 246, 1067, 1516, 1709, 1856, 2432
BsmAI GTCTC 9 cut(s) 73, 701, 720, 923, 1485, 1619, 1872, 2048, 2216
BsmFI GGGAC 5 cut(s) 250, 378, 413, 584, 2501
BsnI GGCC 5 cut(s) 116, 1172, 1925, 2360, 2499
Bso31I GGTCTC 2 cut(s) 73, 701
Bsp119I TTCGAA 1 cut(s) 831
Bsp1286I GDGCHC 1 cut(s) 1736
Bsp13I TCCGGA 1 cut(s) 1030
Bsp1407I TGTACA 1 cut(s) 1588
Bsp1720I GCTNAGC 1 cut(s) 1087
BspANI GGCC 5 cut(s) 116, 1172, 1925, 2360, 2499
BspCNI CTCAG 5 cut(s) 880, 907, 1100, 1699, 2095
BspDI ATCGAT 1 cut(s) 1430
BspEI TCCGGA 1 cut(s) 1030
BspHI TCATGA 1 cut(s) 2575
BspMI ACCTGC 2 cut(s) 212, 1378
BspOI GCTAGC 1 cut(s) 1158
BspPI GGATC 4 cut(s) 157, 257, 346, 633
BspQI GCTCTTC 1 cut(s) 828
BspT104I TTCGAA 1 cut(s) 831
BspT107I GGYRCC 5 cut(s) 344, 653, 1696, 1825, 2035
BspTNI GGTCTC 2 cut(s) 73, 701
BsrBI CCGCTC 1 cut(s) 2249
BsrDI GCAATG 4 cut(s) 633, 932, 1422, 1555
BsrFI RCCGGY 2 cut(s) 559, 580
BsrGI TGTACA 1 cut(s) 1588
BsrI ACTGG 5 cut(s) 251, 366, 1026, 2501, 2567
BssAI RCCGGY 2 cut(s) 559, 580
BssECI CCNNGG 6 cut(s) 63, 395, 396, 1955, 2174, 2526
BssNI GRCGYC 3 cut(s) 129, 345, 1697
BssT1I CCWWGG 3 cut(s) 63, 1955, 2526
Bst2UI CCWGG 4 cut(s) 293, 397, 704, 969
Bst4CI ACNGT 6 cut(s) 671, 1239, 1738, 1763, 2515, 2534
Bst6I CTCTTC 1 cut(s) 828
BstACI GRCGYC 3 cut(s) 129, 345, 1697
BstAPI GCANNNNNTGC 1 cut(s) 619
BstAUI TGTACA 1 cut(s) 1588
BstBI TTCGAA 1 cut(s) 831
BstC8I GCNNGC 7 cut(s) 91, 582, 744, 808, 902, 1156, 1642
BstDEI CTNAG 7 cut(s) 791, 888, 915, 1087, 1645, 1686, 2082
BstDSI CCRYGG 1 cut(s) 2174
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 3 cut(s) 105, 1022, 2279
BstH2I RGCGCY 3 cut(s) 348, 1700, 1913
BstHHI GCGC 6 cut(s) 209, 347, 1699, 1912, 2125, 2293
BstMAI GTCTC 9 cut(s) 73, 701, 720, 923, 1485, 1619, 1872, 2048, 2216
BstMCI CGRYCG 4 cut(s) 141, 977, 1173, 2514
BstNI CCWGG 4 cut(s) 293, 397, 704, 969
BstNSI RCATGY 1 cut(s) 810
BstSCI CCNGG 5 cut(s) 291, 395, 702, 967, 1509
BstV1I GCAGC 1 cut(s) 576
BstV2I GAAGAC 1 cut(s) 1220
BstX2I RGATCY 2 cut(s) 162, 351
BstYI RGATCY 2 cut(s) 162, 351
BstZI CGGCCG 1 cut(s) 1170
Bsu15I ATCGAT 1 cut(s) 1430
BsuI GTATCC 2 cut(s) 903, 2008
BsuRI GGCC 5 cut(s) 116, 1172, 1925, 2360, 2499
BsuTUI ATCGAT 1 cut(s) 1430
BtgI CCRYGG 1 cut(s) 2174
BtgZI GCGATG 2 cut(s) 202, 599
BtsCI GGATG 3 cut(s) 105, 1022, 2279
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 3 cut(s) 39, 1743, 2560
BveI ACCTGC 2 cut(s) 212, 1378
Cac8I GCNNGC 7 cut(s) 91, 582, 744, 808, 902, 1156, 1642
CaiI CAGNNNCTG 1 cut(s) 619
CciI TCATGA 1 cut(s) 2575
CfoI GCGC 6 cut(s) 209, 347, 1699, 1912, 2125, 2293
Cfr10I RCCGGY 2 cut(s) 559, 580
Cfr13I GGNCC 7 cut(s) 595, 882, 1033, 1924, 2359, 2488, 2498
ClaI ATCGAT 1 cut(s) 1430
Csp6I GTAC 7 cut(s) 151, 1107, 1589, 1918, 1932, 2007, 2036
CspAI ACCGGT 1 cut(s) 559
CspCI CAANNNNNGTGG 2 cut(s) 1734, 1769
CviQI GTAC 7 cut(s) 151, 1107, 1589, 1918, 1932, 2007, 2036
DdeI CTNAG 7 cut(s) 791, 888, 915, 1087, 1645, 1686, 2082
DinI GGCGCC 2 cut(s) 346, 1698
DrdI GACNNNNNNGTC 1 cut(s) 152
DriI GACNNNNNGTC 1 cut(s) 390
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 1170
EagI CGGCCG 1 cut(s) 1170
Eam1104I CTCTTC 1 cut(s) 828
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 828
EciI GGCGGA 1 cut(s) 2027
EclXI CGGCCG 1 cut(s) 1170
Eco130I CCWWGG 3 cut(s) 63, 1955, 2526
Eco31I GGTCTC 2 cut(s) 73, 701
Eco47I GGWCC 4 cut(s) 595, 882, 1033, 2488
Eco52I CGGCCG 1 cut(s) 1170
Eco57I CTGAAG 2 cut(s) 2280, 2440
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 882
EcoRI GAATTC 2 cut(s) 1247, 1534
EcoRII CCWGG 4 cut(s) 291, 395, 702, 967
EcoT14I CCWWGG 3 cut(s) 63, 1955, 2526
EcoT22I ATGCAT 2 cut(s) 808, 1555
EgeI GGCGCC 2 cut(s) 346, 1698
EheI GGCGCC 2 cut(s) 346, 1698
ErhI CCWWGG 3 cut(s) 63, 1955, 2526
FalI AAGNNNNNCTT 6 cut(s) 1170, 1202, 1371, 1403, 1948, 1980
FaqI GGGAC 5 cut(s) 250, 378, 413, 584, 2501
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 8 cut(s) 198, 479, 590, 657, 980, 983, 1020, 1292
FokI GGATG 3 cut(s) 92, 1009, 2266
Fsp4HI GCNGC 8 cut(s) 198, 479, 590, 657, 980, 983, 1020, 1292
FspBI CTAG 2 cut(s) 59, 1155
FspI TGCGCA 2 cut(s) 2124, 2292
GlaI GCGC 6 cut(s) 208, 346, 1698, 1911, 2124, 2292
GluI GCNGC 8 cut(s) 198, 479, 590, 657, 980, 983, 1020, 1292
GsuI CTGGAG 2 cut(s) 349, 458
HaeII RGCGCY 3 cut(s) 348, 1700, 1913
HaeIII GGCC 5 cut(s) 116, 1172, 1925, 2360, 2499
HapII CCGG 8 cut(s) 465, 560, 581, 598, 651, 1031, 1244, 1511
HhaI GCGC 6 cut(s) 209, 347, 1699, 1912, 2125, 2293
Hin1I GRCGYC 3 cut(s) 129, 345, 1697
Hin6I GCGC 6 cut(s) 207, 345, 1697, 1910, 2123, 2291
HinP1I GCGC 6 cut(s) 207, 345, 1697, 1910, 2123, 2291
HincII GTYRAC 1 cut(s) 2506
HindII GTYRAC 1 cut(s) 2506
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 8 cut(s) 285, 380, 601, 994, 1274, 1394, 1496, 2422
HpaI GTTAAC 1 cut(s) 2506
HpaII CCGG 8 cut(s) 465, 560, 581, 598, 651, 1031, 1244, 1511
HphI GGTGA 7 cut(s) 454, 478, 1227, 1373, 1944, 2100, 2268
Hpy166II GTNNAC 1 cut(s) 2506
Hpy8I GTNNAC 1 cut(s) 2506
Hpy99I CGWCG 7 cut(s) 131, 134, 146, 257, 543, 1114, 1504
HpyAV CCTTC 8 cut(s) 13, 421, 1352, 1476, 1686, 2087, 2255, 2464
HpyCH4III ACNGT 6 cut(s) 671, 1239, 1738, 1763, 2515, 2534
HpyCH4IV ACGT 6 cut(s) 129, 153, 340, 1109, 1916, 2408
HpyF3I CTNAG 7 cut(s) 791, 888, 915, 1087, 1645, 1686, 2082
HpySE526I ACGT 6 cut(s) 129, 153, 340, 1109, 1916, 2408
Hsp92I GRCGYC 3 cut(s) 129, 345, 1697
HspAI GCGC 6 cut(s) 207, 345, 1697, 1910, 2123, 2291
KasI GGCGCC 2 cut(s) 344, 1696
Kpn2I TCCGGA 1 cut(s) 1030
KpnI GGTACC 1 cut(s) 2039
KroI GCCGGC 1 cut(s) 580
KroNI GCCGGC 1 cut(s) 582
KspAI GTTAAC 1 cut(s) 2506
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 7 cut(s) 477, 919, 1057, 1731, 1747, 1999, 2416
Lsp1109I GCAGC 1 cut(s) 576
LweI GCATC 4 cut(s) 237, 576, 1031, 1082
MaeI CTAG 2 cut(s) 59, 1155
MaeII ACGT 6 cut(s) 129, 153, 340, 1109, 1916, 2408
MaeIII GTNAC 8 cut(s) 100, 530, 1233, 1239, 1634, 1782, 1950, 2106
MbiI CCGCTC 1 cut(s) 2249
MboII GAAGA 7 cut(s) 280, 452, 845, 1225, 1568, 1788, 1956
MfeI CAATTG 1 cut(s) 1202
MflI RGATCY 2 cut(s) 162, 351
MhlI GDGCHC 1 cut(s) 1736
Mly113I GGCGCC 2 cut(s) 345, 1697
MlyI GAGTC 3 cut(s) 374, 1403, 2416
MmeI TCCRAC 5 cut(s) 105, 216, 1473, 1769, 2021
Mph1103I ATGCAT 2 cut(s) 808, 1555
MroI TCCGGA 1 cut(s) 1030
MroNI GCCGGC 1 cut(s) 580
MroXI GAANNNNTTC 2 cut(s) 827, 1383
MseI TTAA 6 cut(s) 14, 357, 641, 674, 1005, 2505
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 8 cut(s) 465, 560, 581, 598, 651, 1031, 1244, 1511
MspR9I CCNGG 5 cut(s) 293, 397, 704, 969, 1511
MunI CAATTG 1 cut(s) 1202
MvaI CCWGG 4 cut(s) 293, 397, 704, 969
NaeI GCCGGC 1 cut(s) 582
NarI GGCGCC 2 cut(s) 345, 1697
NciI CCSGG 1 cut(s) 1511
NgoMIV GCCGGC 1 cut(s) 580
NheI GCTAGC 1 cut(s) 1154
NmeAIII GCCGAG 1 cut(s) 1198
NmuCI GTSAC 3 cut(s) 1233, 1950, 2106
NsbI TGCGCA 2 cut(s) 2124, 2292
NsiI ATGCAT 2 cut(s) 808, 1555
NspI RCATGY 1 cut(s) 810
NspV TTCGAA 1 cut(s) 831
PaeI GCATGC 1 cut(s) 810
PagI TCATGA 1 cut(s) 2575
PaqCI CACCTGC 1 cut(s) 212
PasI CCCWGGG 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 828
PcsI WCGNNNNNNNCGW 4 cut(s) 93, 138, 150, 1106
PdiI GCCGGC 1 cut(s) 582
PdmI GAANNNNTTC 2 cut(s) 827, 1383
PfeI GAWTC 5 cut(s) 285, 601, 994, 1274, 1496
PflMI CCANNNNNTGG 1 cut(s) 1709
PfoI TCCNGGA 2 cut(s) 291, 702
PinAI ACCGGT 1 cut(s) 559
PkrI GCNGC 8 cut(s) 199, 480, 591, 658, 981, 984, 1021, 1293
Ple19I CGATCG 2 cut(s) 141, 977
PleI GAGTC 3 cut(s) 374, 1402, 2416
PluTI GGCGCC 2 cut(s) 348, 1700
PpsI GAGTC 3 cut(s) 374, 1402, 2416
PpuMI RGGWCCY 1 cut(s) 882
PsiI TTATAA 1 cut(s) 2522
Psp1406I AACGTT 1 cut(s) 2408
Psp5II RGGWCCY 1 cut(s) 882
Psp6I CCWGG 4 cut(s) 291, 395, 702, 967
PspGI CCWGG 4 cut(s) 291, 395, 702, 967
PspPI GGNCC 7 cut(s) 595, 882, 1033, 1924, 2359, 2488, 2498
PspPPI RGGWCCY 1 cut(s) 882
PstNI CAGNNNCTG 1 cut(s) 619
PsuI RGATCY 2 cut(s) 162, 351
PvuI CGATCG 2 cut(s) 141, 977
RsaI GTAC 7 cut(s) 152, 1108, 1590, 1919, 1933, 2008, 2037
RsaNI GTAC 7 cut(s) 151, 1107, 1589, 1918, 1932, 2007, 2036
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 6 cut(s) 14, 357, 641, 674, 1005, 2505
SatI GCNGC 8 cut(s) 198, 479, 590, 657, 980, 983, 1020, 1292
Sau96I GGNCC 7 cut(s) 595, 882, 1033, 1924, 2359, 2488, 2498
SchI GAGTC 3 cut(s) 374, 1403, 2416
ScrFI CCNGG 5 cut(s) 293, 397, 704, 969, 1511
SduI GDGCHC 1 cut(s) 1736
SfaNI GCATC 4 cut(s) 237, 576, 1031, 1082
SfoI GGCGCC 2 cut(s) 346, 1698
SfuI TTCGAA 1 cut(s) 831
SinI GGWCC 4 cut(s) 595, 882, 1033, 2488
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SphI GCATGC 1 cut(s) 810
SspDI GGCGCC 2 cut(s) 344, 1696
SspMI CTAG 2 cut(s) 59, 1155
StyD4I CCNGG 5 cut(s) 291, 395, 702, 967, 1509
StyI CCWWGG 3 cut(s) 63, 1955, 2526
TaaI ACNGT 6 cut(s) 671, 1239, 1738, 1763, 2515, 2534
TaiI ACGT 6 cut(s) 132, 156, 343, 1112, 1919, 2411
TatI WGTACW 2 cut(s) 1588, 1931
TauI GCSGC 7 cut(s) 200, 481, 659, 982, 985, 1022, 1294
TfiI GAWTC 5 cut(s) 285, 601, 994, 1274, 1496
Tru1I TTAA 6 cut(s) 14, 357, 641, 674, 1005, 2505
Tru9I TTAA 6 cut(s) 14, 357, 641, 674, 1005, 2505
TscAI CASTG 3 cut(s) 39, 1743, 2567
TseFI GTSAC 3 cut(s) 1233, 1950, 2106
TseI GCWGC 1 cut(s) 589
Tsp45I GTSAC 3 cut(s) 1233, 1950, 2106
TspGWI ACGGA 3 cut(s) 146, 1215, 2163
TspRI CASTG 3 cut(s) 39, 1743, 2567
Van91I CCANNNNNTGG 1 cut(s) 1709
VpaK11BI GGWCC 4 cut(s) 595, 882, 1033, 2488
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 7 cut(s) 909, 1247, 1534, 1651, 2212, 2336, 2380
XceI RCATGY 1 cut(s) 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 1513
XmnI GAANNNNTTC 2 cut(s) 827, 1383
XspI CTAG 2 cut(s) 59, 1155
ZraI GACGTC 1 cut(s) 130
Zsp2I ATGCAT 2 cut(s) 808, 1555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.