Rroxscaffold_2G00093410

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005991
Physical Location & Seq
Reverse (-)
14766111 .. 14769183
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00093410.1

Sequence Viewer

Length: 1503 bp
ATGGCTTTACGGTTGGGGCATTGTTCTTCTTTTCTTGAACCTAATTCTTTCAAGCAAGAGGGCTTGACAACAAAGAGGCTATTGAGCAGTTGGGGTTTTCAGAAAACTTCAAGCGCTCAATACAAGCGTTTTGAGATCAGAGCTTCGGCTTCCAATGTGCAACCGCTGAATGCTGCTTCTTTGCAGGCTGGTCCAACAGCAAATGTCTTGGATGCTGAACTTGTCGAAACTCCAGCTAATAAGGGGTTCAGTCCATTCTTGGAAACAGAGAAGGACAAGTCTACTCTCAGCATTGTAGTTGTAGGGGCTTCTGGGGATCTTGCTAGAAAGAAGATCTTCCCAGCTCTTTTTGCCCTTTTTTACGATGATCATCTACCTCAGGCTGGAAGAGAATCAAACAGGCTGTTTTACTTGGCAATACCCCCAGATGTGTTTGTGGACGCAACTAGATGTGCTAAACATCGTGCTTCTTCAGAAAATGGATGGACTCGTGTCATTGTTGAAAAGCCCATTTGGTCAATTGACCATCAGTTGGGTGAGGAACTGGTGGAGAATCTCTTGGTACTTCGTTTCTCAAACCTTGTTTTCGAGCCTTTGTGGTCCAGGAACTACATCCGCAGTGTACAGCTGATTTTTTCTGAAGACTTAGGGGTAGAAGGGAGAGGCAGAACGTTGCCCAACTGTACTAACATTTATATAGATACTTTGAAAATTATGGTATCATCCGGGGATATAATGCAAAACCATCTCCTCCAAATATTGTCACTGTTTGCAATGGACACACCTGTCAGCTTAGATGCTGAAGATATACGGAATGAAAAGGTTAAGGTTCTACGTTCCATTAAACCTGTTGAACTCAAAGATGTCGTTGTGGGTCAATATAAGCGCCATTGCGAGGGGAATCTTTGCTATCCTGCTTATACTGATGATCCAACTGTTTCAAATGATAGCCTCACACCAACTTTTGCAGCCGCTACTATGTTTATTAATAATTCACGATGGGACGGAGTTCCAATCATGCTGATAGCTGGCAAGGCTCTTCAGTCAAAACGAGCGGAAATCAGAGTTCAATTCAGACATGTCCCGGGAAACTTGTATAATCGGAACTCTGGAACTGACTTGGATAAGGCTACAAATGAGCTCGTGCTTCGTATACAACCTGAAGAAGCTGTCTATTTGAAGGTTAACAACAAGGTTCCTGGACTTGGAATGAGGCTGGACCGTAGTGACCTTAATTTGCTGTATAAATCAAGGTATCCAAGAGAGTTCCCAGATGCATATGAGAGGCTTCTTTTAGATGCAATGAAAGGAGAGCGACGATCATTTATTAGAAGCGATGAGCTTGAAGCAGCTTGGGCAGTATTCACTCCATTGTTGAAGGAATTGGAAGAGAAGAAAGTTATGCCAGAGCTGTACAGCTATGGTAGTAGTGGACCTGTCGGAGTGCATTATCTTGCTGCAGAGCACAATGTTCGATTAGGAGATTTTGGTTACGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00030 ko00480 ko01100 ko01110 ko01120 ko01130 ko01200 ko05230 map00030 map00480 map01100 map01110 map01120 map01130 map01200 map05230
Pfam Domains
Protein Families
G6PD_C G6PD_N

Protein Analysis

500

Amino Acids

56.31

Weight (kDa)

6.36

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G6PD_N PF00479 99 - 128 1.7e-06 Glucose-6-phosphate dehydrogenase, NAD binding domain
G6PD_N PF00479 130 - 171 1.1e-07 Glucose-6-phosphate dehydrogenase, NAD binding domain
G6PD_C PF02781 187 - 490 5.6e-97 Glucose-6-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 785
AccB7I CCANNNNNTGG 1 cut(s) 532
AccBSI CCGCTC 1 cut(s) 1055
AccI GTMKAC 2 cut(s) 281, 1153
AciI CCGC 4 cut(s) 164, 616, 972, 1055
AclI AACGTT 1 cut(s) 671
AclWI GGATC 2 cut(s) 324, 923
AcuI CTGAAG 5 cut(s) 456, 660, 822, 1025, 1182
AfaI GTAC 4 cut(s) 564, 624, 685, 1415
AfeI AGCGCT 1 cut(s) 115
AfiI CCNNNNNNNGG 4 cut(s) 383, 532, 895, 1205
AflIII ACRYGT 1 cut(s) 1078
AjnI CCWGG 2 cut(s) 602, 1198
Alw21I GWGCWC 2 cut(s) 1143, 1467
AlwI GGATC 2 cut(s) 324, 923
Ama87I CYCGRG 1 cut(s) 1084
Aor51HI AGCGCT 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 173, 968, 1349, 1457
AseI ATTAAT 1 cut(s) 987
Asp700I GAANNNNTTC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 116, 888
AspS9I GGNCC 4 cut(s) 191, 600, 1219, 1433
AsuC2I CCSGG 3 cut(s) 727, 1085, 1086
AsuHPI GGTGA 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 1084
AvaII GGWCC 4 cut(s) 191, 600, 1219, 1433
AxyI CCTNAGG 1 cut(s) 378
BanII GRGCYC 1 cut(s) 1143
BauI CACGAG 2 cut(s) 489, 1142
BbsI GAAGAC 1 cut(s) 648
Bbv12I GWGCWC 2 cut(s) 1143, 1467
BbvI GCAGC 4 cut(s) 160, 980, 1361, 1444
BccI CCATC 4 cut(s) 477, 534, 753, 993
BcgI CGANNNNNNTGC 2 cut(s) 1454, 1488
BciT130I CCWGG 2 cut(s) 604, 1200
BciVI GTATCC 1 cut(s) 1266
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 3 cut(s) 727, 1085, 1086
BfaI CTAG 2 cut(s) 324, 447
BfmI CTRYAG 1 cut(s) 1458
BfoI RGCGCY 2 cut(s) 117, 889
BfuI GTATCC 1 cut(s) 1266
BglII AGATCT 1 cut(s) 333
BisI GCNGC 5 cut(s) 174, 969, 972, 1350, 1458
BlsI GCNGC 5 cut(s) 175, 970, 973, 1351, 1459
Bme1390I CCNGG 5 cut(s) 604, 727, 1085, 1086, 1200
Bme18I GGWCC 4 cut(s) 191, 600, 1219, 1433
BmeT110I CYCGRG 1 cut(s) 1084
BmgT120I GGNCC 4 cut(s) 191, 600, 1219, 1433
BmiI GGNNCC 1 cut(s) 1197
BmrFI CCNGG 5 cut(s) 604, 727, 1085, 1086, 1200
BmsI GCATC 4 cut(s) 202, 787, 1264, 1288
BoxI GACNNNNGTC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 648
BpmI CTGGAG 1 cut(s) 216
BpuMI CCSGG 3 cut(s) 727, 1085, 1086
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 726, 1084
Bsc4I CCNNNNNNNGG 4 cut(s) 383, 532, 895, 1205
Bse1I ACTGG 1 cut(s) 549
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 3 cut(s) 780, 889, 1308
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 2 cut(s) 604, 1200
BseDI CCNNGG 2 cut(s) 726, 1084
BseGI GGATG 4 cut(s) 217, 488, 612, 722
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 4 cut(s) 383, 532, 895, 1205
BseMI GCAATG 3 cut(s) 780, 889, 1308
BseMII CTCAG 2 cut(s) 301, 392
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 1 cut(s) 740
BseXI GCAGC 4 cut(s) 160, 980, 1361, 1444
BseYI CCCAGC 1 cut(s) 340
BsiHKAI GWGCWC 2 cut(s) 1143, 1467
BsiHKCI CYCGRG 1 cut(s) 1084
BsiSI CCGG 2 cut(s) 726, 1085
BslFI GGGAC 2 cut(s) 1016, 1067
BslI CCNNNNNNNGG 4 cut(s) 383, 532, 895, 1205
BsmFI GGGAC 2 cut(s) 1016, 1067
BsmI GAATGC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 1084
Bsp1286I GDGCHC 2 cut(s) 1143, 1467
Bsp1407I TGTACA 2 cut(s) 622, 1413
Bsp143I GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
BspACI CCGC 4 cut(s) 164, 616, 972, 1055
BspCNI CTCAG 2 cut(s) 300, 391
BspLI GGNNCC 1 cut(s) 1197
BspMAI CTGCAG 1 cut(s) 1462
BspPI GGATC 2 cut(s) 324, 923
BspQI GCTCTTC 1 cut(s) 1044
BsrBI CCGCTC 1 cut(s) 1055
BsrDI GCAATG 3 cut(s) 780, 889, 1308
BsrGI TGTACA 2 cut(s) 622, 1413
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 2 cut(s) 726, 1084
BssMI GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
BssNAI GTATAC 1 cut(s) 1154
BssSI CACGAG 2 cut(s) 489, 1142
Bst1107I GTATAC 1 cut(s) 1154
Bst2BI CACGAG 2 cut(s) 489, 1142
Bst2UI CCWGG 2 cut(s) 604, 1200
Bst4CI ACNGT 5 cut(s) 12, 683, 768, 937, 1223
Bst6I CTCTTC 3 cut(s) 382, 1044, 1383
BstAUI TGTACA 2 cut(s) 622, 1413
BstC8I GCNNGC 2 cut(s) 186, 1030
BstDEI CTNAG 4 cut(s) 287, 378, 646, 793
BstF5I GGATG 4 cut(s) 217, 488, 612, 722
BstH2I RGCGCY 2 cut(s) 117, 889
BstHHI GCGC 2 cut(s) 116, 888
BstKTI GATC 6 cut(s) 138, 319, 336, 370, 931, 1322
BstMBI GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
BstMWI GCNNNNNNNGC 3 cut(s) 350, 1034, 1355
BstNI CCWGG 2 cut(s) 604, 1200
BstNSI RCATGY 1 cut(s) 1082
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 5 cut(s) 602, 725, 1083, 1084, 1198
BstSFI CTRYAG 1 cut(s) 1458
BstV1I GCAGC 4 cut(s) 160, 980, 1361, 1444
BstV2I GAAGAC 1 cut(s) 648
BstX2I RGATCY 2 cut(s) 316, 333
BstYI RGATCY 2 cut(s) 316, 333
BstZ17I GTATAC 1 cut(s) 1154
Bsu36I CCTNAGG 1 cut(s) 378
BsuI GTATCC 1 cut(s) 1266
BtgZI GCGATG 1 cut(s) 1350
BtsCI GGATG 4 cut(s) 217, 488, 612, 722
BtsI GCAGTG 1 cut(s) 625
BtsIMutI CAGTG 2 cut(s) 625, 764
Cac8I GCNNGC 2 cut(s) 186, 1030
CfoI GCGC 2 cut(s) 116, 888
Cfr13I GGNCC 4 cut(s) 191, 600, 1219, 1433
Cfr9I CCCGGG 1 cut(s) 1084
CseI GACGC 1 cut(s) 449
Csp6I GTAC 4 cut(s) 563, 623, 684, 1414
CviAII CATG 2 cut(s) 1018, 1079
CviQI GTAC 4 cut(s) 563, 623, 684, 1414
DdeI CTNAG 4 cut(s) 287, 378, 646, 793
DpnI GATC 6 cut(s) 137, 318, 335, 369, 930, 1321
DpnII GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
DrdI GACNNNNNNGTC 1 cut(s) 785
DseDI GACNNNNNNGTC 1 cut(s) 785
Eam1104I CTCTTC 3 cut(s) 382, 1044, 1383
EarI CTCTTC 3 cut(s) 382, 1044, 1383
Ecl136II GAGCTC 1 cut(s) 1141
Eco24I GRGCYC 1 cut(s) 1143
Eco47I GGWCC 4 cut(s) 191, 600, 1219, 1433
Eco47III AGCGCT 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 1141
Eco57I CTGAAG 5 cut(s) 456, 660, 822, 1025, 1182
Eco81I CCTNAGG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 1084
EcoICRI GAGCTC 1 cut(s) 1141
EcoRII CCWGG 2 cut(s) 602, 1198
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 1143
FaeI CATG 2 cut(s) 1021, 1082
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 2 cut(s) 1016, 1067
FatI CATG 2 cut(s) 1017, 1078
FauNDI CATATG 1 cut(s) 1279
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 2 cut(s) 281, 1153
Fnu4HI GCNGC 5 cut(s) 174, 969, 972, 1350, 1458
FokI GGATG 4 cut(s) 224, 495, 599, 709
FriOI GRGCYC 1 cut(s) 1143
Fsp4HI GCNGC 5 cut(s) 174, 969, 972, 1350, 1458
FspBI CTAG 2 cut(s) 324, 447
GlaI GCGC 2 cut(s) 115, 887
GluI GCNGC 5 cut(s) 174, 969, 972, 1350, 1458
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 2 cut(s) 117, 889
HapII CCGG 2 cut(s) 726, 1085
HgaI GACGC 1 cut(s) 449
HhaI GCGC 2 cut(s) 116, 888
Hin1II CATG 2 cut(s) 1021, 1082
Hin6I GCGC 2 cut(s) 114, 886
HinP1I GCGC 2 cut(s) 114, 886
HincII GTYRAC 1 cut(s) 1186
HindII GTYRAC 1 cut(s) 1186
HinfI GANTC 4 cut(s) 392, 487, 553, 901
HpaI GTTAAC 1 cut(s) 1186
HpaII CCGG 2 cut(s) 726, 1085
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 6 cut(s) 282, 439, 623, 1154, 1186, 1433
Hpy188I TCNGA 8 cut(s) 102, 140, 475, 640, 1064, 1076, 1104, 1442
Hpy188III TCNNGA 3 cut(s) 35, 996, 1110
Hpy8I GTNNAC 6 cut(s) 282, 439, 623, 1154, 1186, 1433
Hpy99I CGWCG 1 cut(s) 1320
HpyAV CCTTC 4 cut(s) 265, 650, 1174, 1372
HpyCH4III ACNGT 5 cut(s) 12, 683, 768, 937, 1223
HpyCH4IV ACGT 2 cut(s) 671, 835
HpyCH4V TGCA 9 cut(s) 160, 184, 739, 773, 968, 1277, 1301, 1447, 1460
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 1034, 1355
HpyF3I CTNAG 4 cut(s) 287, 378, 646, 793
HpySE526I ACGT 2 cut(s) 671, 835
Hsp92II CATG 2 cut(s) 1021, 1082
HspAI GCGC 2 cut(s) 114, 886
Ksp22I TGATCA 1 cut(s) 367
KspAI GTTAAC 1 cut(s) 1186
Kzo9I GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
LguI GCTCTTC 1 cut(s) 1044
Lsp1109I GCAGC 4 cut(s) 160, 980, 1361, 1444
LweI GCATC 4 cut(s) 202, 787, 1264, 1288
MaeI CTAG 2 cut(s) 324, 447
MaeII ACGT 2 cut(s) 671, 835
MaeIII GTNAC 3 cut(s) 762, 1226, 1490
MalI GATC 6 cut(s) 137, 318, 335, 369, 930, 1321
MbiI CCGCTC 1 cut(s) 1055
MboI GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 2 cut(s) 316, 333
MhlI GDGCHC 2 cut(s) 1143, 1467
MluCI AATT 7 cut(s) 43, 519, 711, 991, 1070, 1234, 1382
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 3 cut(s) 218, 956, 1420
Mph1103I ATGCAT 1 cut(s) 1279
MroXI GAANNNNTTC 1 cut(s) 335
MseI TTAA 5 cut(s) 825, 843, 987, 1185, 1233
MspA1I CMGCKG 2 cut(s) 166, 628
MspI CCGG 2 cut(s) 726, 1085
MspR9I CCNGG 5 cut(s) 604, 727, 1085, 1086, 1200
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 2 cut(s) 604, 1200
MwoI GCNNNNNNNGC 3 cut(s) 350, 1034, 1355
NciI CCSGG 3 cut(s) 727, 1085, 1086
NdeI CATATG 1 cut(s) 1279
NdeII GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
NlaIII CATG 2 cut(s) 1021, 1082
NlaIV GGNNCC 1 cut(s) 1197
NmuCI GTSAC 2 cut(s) 762, 1226
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 1 cut(s) 1082
PciI ACATGT 1 cut(s) 1078
PciSI GCTCTTC 1 cut(s) 1044
PctI GAATGC 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 3 cut(s) 392, 553, 901
PflMI CCANNNNNTGG 1 cut(s) 532
PfoI TCCNGGA 2 cut(s) 602, 1198
PkrI GCNGC 5 cut(s) 175, 970, 973, 1351, 1459
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PscI ACATGT 1 cut(s) 1078
PshAI GACNNNNGTC 1 cut(s) 491
PshBI ATTAAT 1 cut(s) 987
Psp124BI GAGCTC 1 cut(s) 1143
Psp1406I AACGTT 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 602, 1198
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 2 cut(s) 602, 1198
PspN4I GGNNCC 1 cut(s) 1197
PspPI GGNCC 4 cut(s) 191, 600, 1219, 1433
PstI CTGCAG 1 cut(s) 1462
PsuI RGATCY 2 cut(s) 316, 333
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 4 cut(s) 564, 624, 685, 1415
RsaNI GTAC 4 cut(s) 563, 623, 684, 1414
SacI GAGCTC 1 cut(s) 1143
SapI GCTCTTC 1 cut(s) 1044
SaqAI TTAA 5 cut(s) 825, 843, 987, 1185, 1233
SatI GCNGC 5 cut(s) 174, 969, 972, 1350, 1458
Sau3AI GATC 6 cut(s) 135, 316, 333, 367, 928, 1319
Sau96I GGNCC 4 cut(s) 191, 600, 1219, 1433
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 5 cut(s) 604, 727, 1085, 1086, 1200
SduI GDGCHC 2 cut(s) 1143, 1467
SfaNI GCATC 4 cut(s) 202, 787, 1264, 1288
SfcI CTRYAG 1 cut(s) 1458
SinI GGWCC 4 cut(s) 191, 600, 1219, 1433
SmaI CCCGGG 1 cut(s) 1086
Sse9I AATT 7 cut(s) 43, 519, 711, 991, 1070, 1234, 1382
SsiI CCGC 4 cut(s) 164, 616, 972, 1055
SspI AATATT 1 cut(s) 759
SspMI CTAG 2 cut(s) 324, 447
SstI GAGCTC 1 cut(s) 1143
StyD4I CCNGG 5 cut(s) 602, 725, 1083, 1084, 1198
TaaI ACNGT 5 cut(s) 12, 683, 768, 937, 1223
TaiI ACGT 2 cut(s) 674, 838
TaqI TCGA 3 cut(s) 225, 588, 1474
TasI AATT 7 cut(s) 43, 519, 711, 991, 1070, 1234, 1382
TatI WGTACW 3 cut(s) 622, 683, 1413
TauI GCSGC 1 cut(s) 974
TfiI GAWTC 3 cut(s) 392, 553, 901
Tru1I TTAA 5 cut(s) 825, 843, 987, 1185, 1233
Tru9I TTAA 5 cut(s) 825, 843, 987, 1185, 1233
TscAI CASTG 2 cut(s) 625, 771
TseFI GTSAC 2 cut(s) 762, 1226
TseI GCWGC 4 cut(s) 173, 968, 1349, 1457
Tsp45I GTSAC 2 cut(s) 762, 1226
TspDTI ATGAA 2 cut(s) 831, 1319
TspGWI ACGGA 2 cut(s) 826, 1020
TspMI CCCGGG 1 cut(s) 1084
TspRI CASTG 2 cut(s) 625, 771
Van91I CCANNNNNTGG 1 cut(s) 532
VpaK11BI GGWCC 4 cut(s) 191, 600, 1219, 1433
VspI ATTAAT 1 cut(s) 987
XceI RCATGY 1 cut(s) 1082
XmaI CCCGGG 1 cut(s) 1084
XmiI GTMKAC 2 cut(s) 281, 1153
XmnI GAANNNNTTC 1 cut(s) 335
XspI CTAG 2 cut(s) 324, 447
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.