Rroxscaffold_2G00097290

DNA damage response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011582
Physical Location & Seq
Reverse (-)
18499498 .. 18501627
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097290.1

Sequence Viewer

Length: 960 bp
ATGGATCTCAATGATCTTAACAAGGTTTGGGAAATCAAACCACTGAAGAAAATTGGAGAAGAGGATGCAAGAAAAATCCTTGAGAGAGTTGCAAAGCAAGTGCAGCCTATAATGCGCAAGCGTAAATGGAAAGTCCGAATCCTTTCTGAATTCAGTCCTGCCAACCCGGCTCTTCAGGGGCTCAATATAGGAGGAGGTGCTGAAGTTAAGCTCAGACTGCGAAGGCCAAACAACGAATGGGATTTCTATCCGTATGAACAGATCCTTGATACAATGCTGCACGAGCTCTGCCACAACGAGCATGGTCCTCATAATGCCGACTTTTACAACCTTTTGGACGAGATCAGAAGGATGCATGCTGTATGGATGATTGTCACTTTACTTCGTTCCCATGGTTTTCTGCAGGAATGTGAGGAACTGATGGGTAAAGGGATTACTGGCACTGGACAAGGATTTGATCTTCCTGGGAGACGTTTGGGTGGTTTTTCACGACCTAAGCGTTTAGGTGGGGATAGCAACATTAAGGCTGCTTTGAGCCCAATACAAGCTGCTGCTATGGCTGCAGAAAGGAGACTACATGATGATCTATGGTGTGGGTCAAAGTCTTTAGAGGGTGACACTAATGTCCAGGGTCATGTCGGAACTTCTCAAAAGCCACAAGCTTTCACAGTTACTGATGGAAGATCTACCCAAACTTCTACTCAACCAAAGCCAGGTCCCGAAAAAGTAGATGATCAATCGAAGTGGCAATGTAGTACGTGCACTTTACTAAATGAGGAATTGGCATTGACATGTGCAGCCTGCGCGACTTTAAAAGATAACGCCAAGTTTAAGGTTTGGTCTTGCAAATTTTGTACATTAGAAAACAGAGTTGGGGTAGAAAGATGCTTGGCTTGTGGAGAATGGAGATACTCATATGGTCCTCCTGTCTCCAATCGTGGACCTTATGTTGGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WLM zf-RanBP

Protein Analysis

319

Amino Acids

35.82

Weight (kDa)

8.7

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WLM PF08325 8 - 192 6.2e-50 WLM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 623
Acc16I TGCGCA 1 cut(s) 116
AccII CGCG 1 cut(s) 806
AclWI GGATC 2 cut(s) 12, 256
AcsI RAATTY 2 cut(s) 149, 848
AcuI CTGAAG 3 cut(s) 65, 158, 222
AfaI GTAC 2 cut(s) 757, 856
AfiI CCNNNNNNNGG 2 cut(s) 713, 950
AflIII ACRYGT 1 cut(s) 791
AjnI CCWGG 3 cut(s) 463, 627, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 855, 887
AluBI AGCT 4 cut(s) 211, 286, 548, 662
AluI AGCT 4 cut(s) 211, 286, 548, 662
Alw21I GWGCWC 2 cut(s) 288, 764
Alw26I GTCTC 3 cut(s) 463, 565, 934
Alw44I GTGCAC 1 cut(s) 760
AlwI GGATC 2 cut(s) 12, 256
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 1 cut(s) 224
ApaLI GTGCAC 1 cut(s) 760
ApeKI GCWGC 7 cut(s) 103, 277, 527, 548, 551, 560, 797
ApoI RAATTY 2 cut(s) 149, 848
Asp700I GAANNNNTTC 1 cut(s) 142
AspLEI GCGC 2 cut(s) 117, 806
AspS9I GGNCC 4 cut(s) 305, 716, 920, 941
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 626
AvaII GGWCC 4 cut(s) 305, 716, 920, 941
BaeGI GKGCMC 1 cut(s) 764
BanII GRGCYC 3 cut(s) 183, 288, 539
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 288, 764
BbvI GCAGC 7 cut(s) 115, 264, 514, 535, 538, 547, 809
BccI CCATC 2 cut(s) 415, 671
BciT130I CCWGG 3 cut(s) 465, 629, 714
BclI TGATCA 1 cut(s) 733
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 3 cut(s) 463, 565, 934
BfmI CTRYAG 2 cut(s) 401, 561
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 683
BisI GCNGC 7 cut(s) 104, 278, 528, 549, 552, 561, 798
BlsI GCNGC 7 cut(s) 105, 279, 529, 550, 553, 562, 799
Bme1390I CCNGG 4 cut(s) 167, 465, 629, 714
Bme18I GGWCC 4 cut(s) 305, 716, 920, 941
BmgT120I GGNCC 4 cut(s) 305, 716, 920, 941
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 4 cut(s) 167, 465, 629, 714
BmsI GCATC 3 cut(s) 55, 342, 875
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 759
BsaBI GATNNNNATC 1 cut(s) 246
BsaJI CCNNGG 3 cut(s) 391, 464, 628
Bsc4I CCNNNNNNNGG 2 cut(s) 713, 950
Bse1I ACTGG 2 cut(s) 442, 448
Bse3DI GCAATG 1 cut(s) 755
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 3 cut(s) 465, 629, 714
BseDI CCNNGG 3 cut(s) 391, 464, 628
BseGI GGATG 3 cut(s) 70, 357, 372
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 713, 950
BseMI GCAATG 1 cut(s) 755
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 2 cut(s) 442, 448
BseRI GAGGAG 1 cut(s) 207
BseSI GKGCMC 1 cut(s) 764
BseXI GCAGC 7 cut(s) 115, 264, 514, 535, 538, 547, 809
BsgI GTGCAG 3 cut(s) 122, 263, 816
Bsh1236I CGCG 1 cut(s) 806
BshFI GGCC 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 288, 764
BsiSI CCGG 1 cut(s) 167
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 2 cut(s) 713, 950
BsmAI GTCTC 3 cut(s) 463, 565, 934
BsmBI CGTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 1 cut(s) 226
Bsp1286I GDGCHC 4 cut(s) 183, 288, 539, 764
Bsp1407I TGTACA 1 cut(s) 854
Bsp143I GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
Bsp19I CCATGG 1 cut(s) 391
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 806
BspLI GGNNCC 1 cut(s) 718
BspMAI CTGCAG 2 cut(s) 405, 565
BspPI GGATC 2 cut(s) 12, 256
BspQI GCTCTTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 755
BsrGI TGTACA 1 cut(s) 854
BsrI ACTGG 2 cut(s) 442, 448
BssECI CCNNGG 3 cut(s) 391, 464, 628
BssMI GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 391
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 3 cut(s) 465, 629, 714
Bst4CI ACNGT 1 cut(s) 670
Bst6I CTCTTC 2 cut(s) 54, 177
BstAUI TGTACA 1 cut(s) 854
BstBAI YACGTR 1 cut(s) 759
BstC8I GCNNGC 3 cut(s) 119, 357, 802
BstDEI CTNAG 2 cut(s) 212, 495
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 3 cut(s) 70, 357, 372
BstFNI CGCG 1 cut(s) 806
BstHHI GCGC 2 cut(s) 117, 806
BstKTI GATC 8 cut(s) 7, 16, 264, 345, 460, 586, 686, 736
BstMAI GTCTC 3 cut(s) 463, 565, 934
BstMBI GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
BstMWI GCNNNNNNNGC 8 cut(s) 103, 112, 167, 217, 283, 557, 560, 803
BstNI CCWGG 3 cut(s) 465, 629, 714
BstNSI RCATGY 2 cut(s) 359, 795
BstSCI CCNGG 4 cut(s) 165, 463, 627, 712
BstSFI CTRYAG 2 cut(s) 401, 561
BstSLI GKGCMC 1 cut(s) 764
BstUI CGCG 1 cut(s) 806
BstV1I GCAGC 7 cut(s) 115, 264, 514, 535, 538, 547, 809
BstX2I RGATCY 3 cut(s) 4, 261, 683
BstYI RGATCY 3 cut(s) 4, 261, 683
BsuRI GGCC 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 391
BtsCI GGATG 3 cut(s) 70, 357, 372
BtsIMutI CAGTG 2 cut(s) 41, 441
Cac8I GCNNGC 3 cut(s) 119, 357, 802
CaiI CAGNNNCTG 1 cut(s) 674
CfoI GCGC 2 cut(s) 117, 806
Cfr13I GGNCC 4 cut(s) 305, 716, 920, 941
Csp6I GTAC 2 cut(s) 756, 855
CspCI CAANNNNNGTGG 2 cut(s) 725, 760
CviAII CATG 6 cut(s) 302, 356, 392, 578, 635, 792
CviQI GTAC 2 cut(s) 756, 855
DdeI CTNAG 2 cut(s) 212, 495
DpnI GATC 8 cut(s) 6, 15, 263, 344, 459, 585, 685, 735
DpnII GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
DraI TTTAAA 1 cut(s) 813
DrdI GACNNNNNNGTC 1 cut(s) 623
DseDI GACNNNNNNGTC 1 cut(s) 623
Eam1104I CTCTTC 2 cut(s) 54, 177
EarI CTCTTC 2 cut(s) 54, 177
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 3 cut(s) 183, 288, 539
Eco47I GGWCC 4 cut(s) 305, 716, 920, 941
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 3 cut(s) 65, 158, 222
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 716
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 463, 627, 712
EcoT14I CCWWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 357
EcoT38I GRGCYC 3 cut(s) 183, 288, 539
ErhI CCWWGG 1 cut(s) 391
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 6 cut(s) 305, 359, 395, 581, 638, 795
FaqI GGGAC 1 cut(s) 702
FatI CATG 6 cut(s) 301, 355, 391, 577, 634, 791
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 7 cut(s) 104, 278, 528, 549, 552, 561, 798
FokI GGATG 3 cut(s) 77, 364, 379
FriOI GRGCYC 3 cut(s) 183, 288, 539
Fsp4HI GCNGC 7 cut(s) 104, 278, 528, 549, 552, 561, 798
FspI TGCGCA 1 cut(s) 116
GlaI GCGC 2 cut(s) 116, 805
GluI GCNGC 7 cut(s) 104, 278, 528, 549, 552, 561, 798
HaeIII GGCC 1 cut(s) 226
HapII CCGG 1 cut(s) 167
HhaI GCGC 2 cut(s) 117, 806
Hin1II CATG 6 cut(s) 305, 359, 395, 581, 638, 795
Hin6I GCGC 2 cut(s) 115, 804
HinP1I GCGC 2 cut(s) 115, 804
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 1 cut(s) 138
HpaII CCGG 1 cut(s) 167
HphI GGTGA 1 cut(s) 626
Hpy166II GTNNAC 2 cut(s) 762, 941
Hpy188I TCNGA 5 cut(s) 137, 148, 215, 347, 641
Hpy188III TCNNGA 2 cut(s) 489, 719
Hpy8I GTNNAC 2 cut(s) 762, 941
HpyAV CCTTC 2 cut(s) 216, 342
HpyCH4III ACNGT 1 cut(s) 670
HpyCH4IV ACGT 2 cut(s) 472, 758
HpyF10VI GCNNNNNNNGC 8 cut(s) 103, 112, 167, 217, 283, 557, 560, 803
HpyF3I CTNAG 2 cut(s) 212, 495
HpySE526I ACGT 2 cut(s) 472, 758
Hsp92II CATG 6 cut(s) 305, 359, 395, 581, 638, 795
HspAI GCGC 2 cut(s) 115, 804
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
LguI GCTCTTC 1 cut(s) 177
Lsp1109I GCAGC 7 cut(s) 115, 264, 514, 535, 538, 547, 809
LweI GCATC 3 cut(s) 55, 342, 875
MaeII ACGT 2 cut(s) 472, 758
MaeIII GTNAC 3 cut(s) 373, 614, 670
MalI GATC 8 cut(s) 6, 15, 263, 344, 459, 585, 685, 735
MboI GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
MboII GAAGA 5 cut(s) 58, 71, 164, 452, 693
MflI RGATCY 3 cut(s) 4, 261, 683
MhlI GDGCHC 4 cut(s) 183, 288, 539, 764
MluCI AATT 4 cut(s) 51, 149, 779, 848
MmeI TCCRAC 1 cut(s) 619
MnlI CCTC 8 cut(s) 55, 185, 188, 318, 406, 604, 769, 933
Mph1103I ATGCAT 1 cut(s) 357
MroXI GAANNNNTTC 1 cut(s) 142
MseI TTAA 5 cut(s) 18, 207, 522, 812, 831
MslI CAYNNNNRTG 1 cut(s) 790
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 4 cut(s) 167, 465, 629, 714
MvaI CCWGG 3 cut(s) 465, 629, 714
MvnI CGCG 1 cut(s) 806
MwoI GCNNNNNNNGC 8 cut(s) 103, 112, 167, 217, 283, 557, 560, 803
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 1 cut(s) 391
NdeI CATATG 1 cut(s) 916
NdeII GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
NlaIII CATG 6 cut(s) 305, 359, 395, 581, 638, 795
NlaIV GGNNCC 1 cut(s) 718
NmuCI GTSAC 2 cut(s) 373, 614
NsbI TGCGCA 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 2 cut(s) 359, 795
PaeI GCATGC 1 cut(s) 359
PciI ACATGT 1 cut(s) 791
PciSI GCTCTTC 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 138
PkrI GCNGC 7 cut(s) 105, 279, 529, 550, 553, 562, 799
Ppu21I YACGTR 1 cut(s) 759
PpuMI RGGWCCY 1 cut(s) 716
PscI ACATGT 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 3 cut(s) 463, 627, 712
PspGI CCWGG 3 cut(s) 463, 627, 712
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 4 cut(s) 305, 716, 920, 941
PspPPI RGGWCCY 1 cut(s) 716
PstI CTGCAG 2 cut(s) 405, 565
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 3 cut(s) 4, 261, 683
RsaI GTAC 2 cut(s) 757, 856
RsaNI GTAC 2 cut(s) 756, 855
RseI CAYNNNNRTG 1 cut(s) 790
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 5 cut(s) 18, 207, 522, 812, 831
SatI GCNGC 7 cut(s) 104, 278, 528, 549, 552, 561, 798
Sau3AI GATC 8 cut(s) 4, 13, 261, 342, 457, 583, 683, 733
Sau96I GGNCC 4 cut(s) 305, 716, 920, 941
ScrFI CCNGG 4 cut(s) 167, 465, 629, 714
SduI GDGCHC 4 cut(s) 183, 288, 539, 764
SfaNI GCATC 3 cut(s) 55, 342, 875
SfcI CTRYAG 2 cut(s) 401, 561
SinI GGWCC 4 cut(s) 305, 716, 920, 941
SmiMI CAYNNNNRTG 1 cut(s) 790
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SphI GCATGC 1 cut(s) 359
Sse9I AATT 4 cut(s) 51, 149, 779, 848
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 4 cut(s) 165, 463, 627, 712
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 1 cut(s) 670
TaiI ACGT 2 cut(s) 475, 761
TaqI TCGA 1 cut(s) 740
TasI AATT 4 cut(s) 51, 149, 779, 848
TatI WGTACW 1 cut(s) 854
TfiI GAWTC 1 cut(s) 138
Tru1I TTAA 5 cut(s) 18, 207, 522, 812, 831
Tru9I TTAA 5 cut(s) 18, 207, 522, 812, 831
TscAI CASTG 2 cut(s) 48, 448
TseFI GTSAC 2 cut(s) 373, 614
TseI GCWGC 7 cut(s) 103, 277, 527, 548, 551, 560, 797
Tsp45I GTSAC 2 cut(s) 373, 614
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 2 cut(s) 48, 448
VneI GTGCAC 1 cut(s) 760
VpaK11BI GGWCC 4 cut(s) 305, 716, 920, 941
XapI RAATTY 2 cut(s) 149, 848
XceI RCATGY 2 cut(s) 359, 795
XcmI CCANNNNNNNNNTGG 2 cut(s) 234, 299
XmnI GAANNNNTTC 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.