Rorug02G0420500

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001741
Physical Location & Seq
Reverse (-)
54023478 .. 54028955
5478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0420500.1

Sequence Viewer

Length: 2229 bp
ATGGGGAGCTCCGGCGAGTCCGGCAACAATGGCATTGACGCGTCGGTGGGTTCCTTGGTTTGGGTCCGGCGCCGAAACGGGTCGTGGTGGCCGGGCCGGATTGTGGGGCTGGACGAGTTGTCCGAGGATTTTGTGGTTTCGCCCAGAGCCGGTACTCCGGTGAAGCTTCTCGGCCGGGACGACGCAAGCGTGGACTGGTATAATCTTGAAAAATCCAAGAGGGTGAAAGCTTTTCGTTGCGGGGAGTATGACGAATGCATTGAAAAAGCAAAGGCTGCTGCAGCTAACCCCAACAAGAAGGCAGTGAAATATGCACGGAGGGAAGACGCCATTATCCATGCTCTTGAGATTGAGAATGAGCGTATAAGCAAGGATAATGTAAATATCTGCTCTCAAATGAGTAATTCAGGTGGTGACCATGATTGCTCAGCCAGAGAATCACCTGCCACGTCTCACTCGGGTGAGGAGAATGGAGATATGACTGATGATGTGAGTCTTTCTGAAGAAAATTCAGACTCGGCTCTGGAATTATCTCAATCTGGTATATCTTTTGAGGAGCCAAATCATATCAGTTCTTCTAAGTTGCAAAGTGTGCAGGGAAGGAGGAGAAGAACACCAAATGATTCAGAGGATGATGGAAATGAAGGGGTTAAGCGCATGAGAGGACTTCAGGACCTGGGAATGGGTGTAGTATCAAAAAGGTCGGTCCAGACTGAAGGGCTTCTTGAAATAGTTCAGCATGACGGTGCTTCAGTCTTAGATACAAACACTCAGAATGGCATGTCCAATGGAAGTCTTGCAAATGGAAGCAAAAATTCCTCATCCTTGAGAAGAAAGAGATCTCAAGTGGCAACAGCTAATGAATTCCTGAAAAGAAAAAATCGTAGCCGACCATTGACAAAGGTTTTGGAGAGTACTGAAATGGTGTCTATTCCAGTTGCAGGTGACCAAGTTCCAAATTCAAGTGGTTCATCTCTTCAATTATCTGATGGCAAGGTTCCTGAATTAGAACCAAATGTGTCAAAAAGGAGTTTATCTATGGACCAGAACGGAGTTTCATGTGATAATGATGCTTCTACAGATGCTCCTGAACAAGCTACAGTTTCTTCCTCTATGAATCAGATGAAAGAGAATGAAATTCTCGGCAAATCTGAGTTATTGGAGAATGGTTCGTCTAAAAGATTCTATGATTTGCGGCTTGTTGGAGAAGAAAAACGCTCTTCAGGTAATTCTGCTGTACTTGCTCCTTGTTCATCTGGAAGGCCTGAAATTGGTGCAATGGCAAGGCAATCTAGTCGAAGTAGTCAAGCTGAAGCTGCATCTTTGAGAAATGAGAGACTGAATGAGTCTGGTCCTACAAGTTCTGCACCCGTGCATGATATTGGCCAAAGTATAGAAAAAGGTAGTTCAAAGTGGCAGTTGAAAGGAAAGAGAAACTCAAGACAAACAAGTAGAGATAGAAAACACAACTCGAGGAAATATATGAACGTCGATGACTTATCCAATGCTGGTTTGGCAGGCATACACCGTTCTGATGTGTTATGTCACGTTTCTGACCTGAAAAGGAATTGCAATGGTATTAGCGGTTCTGCTGCATATAATTACACAGTACAAGGAAAGTCCAAACAGGTTGCGGATTTGTCTGATGGACCGACTCAGAGGTCACTTCCCTATCGGCAGTCTCGCTTCATGGTACCTGCGAGATATCAGACATCAGATTCTATTGCCCGCAATACTTGCTCTGATACTTCATTATATGATGTCAAACTTGAGGTTAAAGCAAAGTACCAGCCACAGCATGTACCACTGGTTTCTCTAATGAGTAAATTGAATGGTAAAGCCATTGTTGGTCACCCAATCACAGTTAAGGCTTTGGATGATGGCTATAGTGACAGACTGTTTGGCAGTACGGAATGTGATCTTAGAGGTGGCAAACTCAGTTATGCAGTTCAGCGAAATGCAGAAATTGGAAGAATCCCAACCAAGCATTTGGGTTCGCCAAAGAAATCTCCCAAAACAAAGAAATCTGGACTGCTACCAAAGAAGACCAGGAAGTTATCTTCACTGACTGGGCACAGGCAATCCGAAGAGAGGAAACTACTGGTAGAGAAGCGAAGGAGTCCTGTAATAGCTTGTATCCCCCTTAAATTAGTTTTCAGTAGGATAAATGAAGCAGTGAGTGGTTTAGCACGACCAACACATCGTGCGTTAAAACCAAGTGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

742

Amino Acids

80.81

Weight (kDa)

9.32

Isoelectric Point (pI)

61.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 17 - 106 1.8e-10 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 451, 932
AasI GACNNNNNNGTC 1 cut(s) 1660
Acc36I ACCTGC 3 cut(s) 451, 932, 1705
Acc65I GGTACC 1 cut(s) 1693
AccB1I GGYRCC 2 cut(s) 69, 1693
AccII CGCG 1 cut(s) 41
AciI CCGC 5 cut(s) 240, 1195, 1584, 1634, 1729
AcoI YGGCCR 3 cut(s) 89, 172, 1384
AcsI RAATTY 5 cut(s) 508, 814, 863, 958, 1137
AcuI CTGAAG 6 cut(s) 522, 653, 735, 735, 1206, 1332
AcyI GRCGYC 2 cut(s) 70, 327
AdeI CACNNNGTG 1 cut(s) 2204
AfaI GTAC 8 cut(s) 154, 916, 1239, 1611, 1695, 1787, 1803, 1909
AfiI CCNNNNNNNGG 7 cut(s) 60, 103, 149, 682, 941, 1271, 2091
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 8 cut(s) 209, 263, 728, 963, 980, 1410, 1423, 1831
AhdI GACNNNNNGTC 1 cut(s) 118
AjiI CACGTC 1 cut(s) 450
AjnI CCWGG 2 cut(s) 675, 2048
AleI CACNNNNGTG 1 cut(s) 459
AluBI AGCT 9 cut(s) 9, 166, 230, 284, 857, 1097, 1310, 1316, 2132
AluI AGCT 9 cut(s) 9, 166, 230, 284, 857, 1097, 1310, 1316, 2132
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 4 cut(s) 456, 1330, 1686, 2216
AlwNI CAGNNNCTG 1 cut(s) 676
Ama87I CYCGRG 2 cut(s) 457, 1471
AoxI GGCC 5 cut(s) 89, 94, 172, 1262, 1384
ApeKI GCWGC 5 cut(s) 275, 278, 281, 1316, 1592
ApoI RAATTY 5 cut(s) 508, 814, 863, 958, 1137
ArsI GACNNNNNNTTYG 4 cut(s) 889, 921, 1883, 1915
Asp700I GAANNNNTTC 2 cut(s) 720, 732
Asp718I GGTACC 1 cut(s) 1693
AspLEI GCGC 2 cut(s) 72, 657
AspS9I GGNCC 7 cut(s) 64, 94, 673, 706, 1042, 1352, 1649
AsuC2I CCSGG 2 cut(s) 93, 176
AsuHPI GGTGA 7 cut(s) 172, 235, 425, 432, 473, 956, 1844
AvaI CYCGRG 2 cut(s) 457, 1471
AvaII GGWCC 6 cut(s) 64, 673, 706, 1042, 1352, 1649
BaeGI GKGCMC 1 cut(s) 2076
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BalI TGGCCA 1 cut(s) 1386
BanI GGYRCC 2 cut(s) 69, 1693
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 330, 2051
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 5 cut(s) 262, 265, 293, 1303, 1579
BccI CCATC 4 cut(s) 629, 983, 1640, 1874
BcgI CGANNNNNNTGC 2 cut(s) 1277, 1311
BciT130I CCWGG 2 cut(s) 677, 2050
BciVI GTATCC 1 cut(s) 2147
BcnI CCSGG 2 cut(s) 93, 176
BcoDI GTCTC 4 cut(s) 456, 1330, 1686, 2216
BfaI CTAG 1 cut(s) 1293
BfmI CTRYAG 4 cut(s) 279, 1077, 1098, 1885
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 3 cut(s) 451, 932, 1705
BfuI GTATCC 1 cut(s) 2147
BglII AGATCT 1 cut(s) 839
BisI GCNGC 6 cut(s) 276, 279, 282, 1196, 1317, 1593
BlpI GCTNAGC 1 cut(s) 427
BlsI GCNGC 6 cut(s) 277, 280, 283, 1197, 1318, 1594
BmcAI AGTACT 1 cut(s) 916
Bme1390I CCNGG 4 cut(s) 93, 176, 677, 2050
Bme18I GGWCC 6 cut(s) 64, 673, 706, 1042, 1352, 1649
BmeRI GACNNNNNGTC 1 cut(s) 118
BmeT110I CYCGRG 2 cut(s) 457, 1471
BmgBI CACGTC 1 cut(s) 450
BmgT120I GGNCC 7 cut(s) 64, 94, 673, 706, 1042, 1352, 1649
BmiI GGNNCC 6 cut(s) 52, 65, 71, 558, 999, 1695
BmrFI CCNGG 4 cut(s) 93, 176, 677, 2050
BmrI ACTGGG 1 cut(s) 2079
BmsI GCATC 3 cut(s) 1060, 1072, 1328
BmuI ACTGGG 1 cut(s) 2079
BpiI GAAGAC 2 cut(s) 330, 2051
Bpu1102I GCTNAGC 1 cut(s) 427
BpuEI CTTGAG 5 cut(s) 365, 828, 847, 1423, 1790
BpuMI CCSGG 2 cut(s) 93, 176
BsaHI GRCGYC 2 cut(s) 70, 327
BsaJI CCNNGG 3 cut(s) 54, 123, 676
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 7 cut(s) 60, 103, 149, 682, 941, 1271, 2091
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 5 cut(s) 200, 935, 1812, 2074, 2106
Bse3DI GCAATG 2 cut(s) 1284, 1579
BseBI CCWGG 2 cut(s) 677, 2050
BseDI CCNNGG 3 cut(s) 54, 123, 676
BseGI GGATG 3 cut(s) 637, 821, 1882
BseLI CCNNNNNNNGG 7 cut(s) 60, 103, 149, 682, 941, 1271, 2091
BseMI GCAATG 2 cut(s) 1284, 1579
BseMII CTCAG 5 cut(s) 441, 785, 1143, 1670, 1951
BseNI ACTGG 5 cut(s) 200, 935, 1812, 2074, 2106
BseRI GAGGAG 3 cut(s) 479, 569, 619
BseSI GKGCMC 1 cut(s) 2076
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 5 cut(s) 262, 265, 293, 1303, 1579
BsgI GTGCAG 2 cut(s) 614, 1350
Bsh1236I CGCG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 5 cut(s) 91, 96, 174, 1264, 1386
BshNI GGYRCC 2 cut(s) 69, 1693
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 2 cut(s) 457, 1471
BsiSI CCGG 8 cut(s) 12, 21, 67, 92, 97, 150, 158, 175
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 7 cut(s) 60, 103, 149, 682, 941, 1271, 2091
BsmAI GTCTC 4 cut(s) 456, 1330, 1686, 2216
BsmBI CGTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 5 cut(s) 91, 96, 174, 1264, 1386
BsoBI CYCGRG 2 cut(s) 457, 1471
Bsp1286I GDGCHC 2 cut(s) 11, 2076
Bsp143I GATC 2 cut(s) 839, 1918
Bsp1720I GCTNAGC 1 cut(s) 427
BspACI CCGC 5 cut(s) 240, 1195, 1584, 1634, 1729
BspANI GGCC 5 cut(s) 91, 96, 174, 1264, 1386
BspCNI CTCAG 5 cut(s) 440, 784, 1144, 1669, 1950
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 6 cut(s) 52, 65, 71, 558, 999, 1695
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 3 cut(s) 451, 932, 1705
BspQI GCTCTTC 1 cut(s) 1225
BspT107I GGYRCC 2 cut(s) 69, 1693
BsrDI GCAATG 2 cut(s) 1284, 1579
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 5 cut(s) 200, 935, 1812, 2074, 2106
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 54, 123, 676
BssMI GATC 2 cut(s) 839, 1918
BssNI GRCGYC 2 cut(s) 70, 327
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 677, 2050
Bst4CI ACNGT 6 cut(s) 746, 1102, 1529, 1609, 1864, 1899
Bst6I CTCTTC 3 cut(s) 981, 1225, 2082
BstACI GRCGYC 2 cut(s) 70, 327
BstAPI GCANNNNNTGC 3 cut(s) 275, 592, 1737
BstC8I GCNNGC 3 cut(s) 187, 1519, 1729
BstDEI CTNAG 8 cut(s) 427, 579, 757, 771, 1152, 1656, 1922, 1937
BstEII GGTNACC 3 cut(s) 413, 944, 1850
BstF5I GGATG 3 cut(s) 637, 821, 1882
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 2 cut(s) 72, 657
BstKTI GATC 2 cut(s) 842, 1921
BstMAI GTCTC 4 cut(s) 456, 1330, 1686, 2216
BstMBI GATC 2 cut(s) 839, 1918
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 9 cut(s) 21, 30, 275, 281, 592, 1241, 1316, 1514, 1737
BstNI CCWGG 2 cut(s) 677, 2050
BstNSI RCATGY 2 cut(s) 784, 1802
BstPI GGTNACC 3 cut(s) 413, 944, 1850
BstSCI CCNGG 4 cut(s) 91, 174, 675, 2048
BstSFI CTRYAG 4 cut(s) 279, 1077, 1098, 1885
BstSLI GKGCMC 1 cut(s) 2076
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 5 cut(s) 262, 265, 293, 1303, 1579
BstV2I GAAGAC 2 cut(s) 330, 2051
BstX2I RGATCY 1 cut(s) 839
BstXI CCANNNNNNTGG 1 cut(s) 1990
BstYI RGATCY 1 cut(s) 839
BstZI CGGCCG 1 cut(s) 172
BsuI GTATCC 1 cut(s) 2147
BsuRI GGCC 5 cut(s) 91, 96, 174, 1264, 1386
BtrI CACGTC 1 cut(s) 450
BtsCI GGATG 3 cut(s) 637, 821, 1882
BtsI GCAGTG 2 cut(s) 309, 2181
BtsIMutI CAGTG 4 cut(s) 309, 1805, 2063, 2181
BveI ACCTGC 3 cut(s) 451, 932, 1705
Cac8I GCNNGC 3 cut(s) 187, 1519, 1729
CaiI CAGNNNCTG 1 cut(s) 676
CfoI GCGC 2 cut(s) 72, 657
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 7 cut(s) 64, 94, 673, 706, 1042, 1352, 1649
CseI GACGC 4 cut(s) 30, 47, 191, 335
Csp6I GTAC 8 cut(s) 153, 915, 1238, 1610, 1694, 1786, 1802, 1908
CviAII CATG 9 cut(s) 338, 419, 658, 740, 781, 1059, 1376, 1690, 1799
CviQI GTAC 8 cut(s) 153, 915, 1238, 1610, 1694, 1786, 1802, 1908
DdeI CTNAG 8 cut(s) 427, 579, 757, 771, 1152, 1656, 1922, 1937
DinI GGCGCC 1 cut(s) 71
DpnI GATC 2 cut(s) 841, 1920
DpnII GATC 2 cut(s) 839, 1918
DraIII CACNNNGTG 1 cut(s) 2204
DrdI GACNNNNNNGTC 1 cut(s) 1660
DriI GACNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 1660
EaeI YGGCCR 3 cut(s) 89, 172, 1384
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 3 cut(s) 981, 1225, 2082
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 3 cut(s) 981, 1225, 2082
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 54
Eco147I AGGCCT 1 cut(s) 1264
Eco24I GRGCYC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 1706
Eco47I GGWCC 6 cut(s) 64, 673, 706, 1042, 1352, 1649
Eco52I CGGCCG 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 6 cut(s) 522, 653, 735, 735, 1206, 1332
Eco88I CYCGRG 2 cut(s) 457, 1471
Eco91I GGTNACC 3 cut(s) 413, 944, 1850
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 673
EcoO65I GGTNACC 3 cut(s) 413, 944, 1850
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 2 cut(s) 675, 2048
EcoRV GATATC 1 cut(s) 1706
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 260
EcoT38I GRGCYC 1 cut(s) 11
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 456
FaeI CATG 9 cut(s) 341, 422, 661, 743, 784, 1062, 1379, 1693, 1802
FalI AAGNNNNNCTT 3 cut(s) 708, 740, 2209
FaqI GGGAC 1 cut(s) 191
FatI CATG 9 cut(s) 337, 418, 657, 739, 780, 1058, 1375, 1689, 1798
FauI CCCGC 2 cut(s) 233, 1736
Fnu4HI GCNGC 6 cut(s) 276, 279, 282, 1196, 1317, 1593
FokI GGATG 3 cut(s) 644, 808, 1889
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 6 cut(s) 276, 279, 282, 1196, 1317, 1593
FspBI CTAG 1 cut(s) 1293
GlaI GCGC 2 cut(s) 71, 656
GluI GCNGC 6 cut(s) 276, 279, 282, 1196, 1317, 1593
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 5 cut(s) 91, 96, 174, 1264, 1386
HapII CCGG 8 cut(s) 12, 21, 67, 92, 97, 150, 158, 175
HgaI GACGC 4 cut(s) 30, 47, 191, 335
HhaI GCGC 2 cut(s) 72, 657
Hin1I GRCGYC 2 cut(s) 70, 327
Hin1II CATG 9 cut(s) 341, 422, 661, 743, 784, 1062, 1379, 1693, 1802
Hin6I GCGC 2 cut(s) 70, 655
HinP1I GCGC 2 cut(s) 70, 655
HindIII AAGCTT 2 cut(s) 164, 228
HpaII CCGG 8 cut(s) 12, 21, 67, 92, 97, 150, 158, 175
HphI GGTGA 7 cut(s) 172, 235, 425, 432, 473, 956, 1844
Hpy166II GTNNAC 1 cut(s) 193
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 3 cut(s) 46, 185, 1493
HpyAV CCTTC 6 cut(s) 292, 594, 638, 710, 1254, 2109
HpyCH4III ACNGT 6 cut(s) 746, 1102, 1529, 1609, 1864, 1899
HpyCH4IV ACGT 3 cut(s) 449, 1488, 1548
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 30, 275, 281, 592, 1241, 1316, 1514, 1737
HpyF3I CTNAG 8 cut(s) 427, 579, 757, 771, 1152, 1656, 1922, 1937
HpySE526I ACGT 3 cut(s) 449, 1488, 1548
Hsp92I GRCGYC 2 cut(s) 70, 327
Hsp92II CATG 9 cut(s) 341, 422, 661, 743, 784, 1062, 1379, 1693, 1802
HspAI GCGC 2 cut(s) 70, 655
KasI GGCGCC 1 cut(s) 69
KpnI GGTACC 1 cut(s) 1697
Kzo9I GATC 2 cut(s) 839, 1918
LguI GCTCTTC 1 cut(s) 1225
LmnI GCTCC 5 cut(s) 6, 14, 556, 1090, 1249
Lsp1109I GCAGC 5 cut(s) 262, 265, 293, 1303, 1579
LweI GCATC 3 cut(s) 1060, 1072, 1328
MaeI CTAG 1 cut(s) 1293
MaeII ACGT 3 cut(s) 449, 1488, 1548
MaeIII GTNAC 6 cut(s) 413, 944, 1544, 1662, 1850, 1889
MalI GATC 2 cut(s) 841, 1920
MboI GATC 2 cut(s) 839, 1918
MflI RGATCY 1 cut(s) 839
MhlI GDGCHC 2 cut(s) 11, 2076
MlsI TGGCCA 1 cut(s) 1386
MluI ACGCGT 1 cut(s) 39
MluNI TGGCCA 1 cut(s) 1386
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 6 cut(s) 26, 502, 509, 1355, 1648, 2128
MmeI TCCRAC 1 cut(s) 1183
Mox20I TGGCCA 1 cut(s) 1386
Mph1103I ATGCAT 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 720, 732
MscI TGGCCA 1 cut(s) 1386
MseI TTAA 5 cut(s) 651, 1776, 1866, 2145, 2210
MslI CAYNNNNRTG 2 cut(s) 459, 744
Msp20I TGGCCA 1 cut(s) 1386
MspI CCGG 8 cut(s) 12, 21, 67, 92, 97, 150, 158, 175
MspR9I CCNGG 4 cut(s) 93, 176, 677, 2050
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 677, 2050
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 9 cut(s) 21, 30, 275, 281, 592, 1241, 1316, 1514, 1737
NarI GGCGCC 1 cut(s) 70
NciI CCSGG 2 cut(s) 93, 176
NdeII GATC 2 cut(s) 839, 1918
NlaIII CATG 9 cut(s) 341, 422, 661, 743, 784, 1062, 1379, 1693, 1802
NlaIV GGNNCC 6 cut(s) 52, 65, 71, 558, 999, 1695
NmeAIII GCCGAG 3 cut(s) 150, 497, 1122
NmuCI GTSAC 6 cut(s) 413, 944, 1544, 1662, 1850, 1889
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 2 cut(s) 784, 1802
OliI CACNNNNGTG 1 cut(s) 459
PaeR7I CTCGAG 1 cut(s) 1471
PaqCI CACCTGC 2 cut(s) 451, 932
PceI AGGCCT 1 cut(s) 1264
PciSI GCTCTTC 1 cut(s) 1225
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 177, 186
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 2 cut(s) 720, 732
PfeI GAWTC 6 cut(s) 437, 623, 1117, 1182, 1718, 1974
PkrI GCNGC 6 cut(s) 277, 280, 283, 1197, 1318, 1594
PleI GAGTC 6 cut(s) 25, 501, 509, 1354, 1648, 2127
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 6 cut(s) 25, 501, 509, 1354, 1648, 2127
PpuMI RGGWCCY 1 cut(s) 673
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 675, 2048
PspEI GGTNACC 3 cut(s) 413, 944, 1850
PspGI CCWGG 2 cut(s) 675, 2048
PspN4I GGNNCC 6 cut(s) 52, 65, 71, 558, 999, 1695
PspPI GGNCC 7 cut(s) 64, 94, 673, 706, 1042, 1352, 1649
PspPPI RGGWCCY 1 cut(s) 673
PspXI VCTCGAGB 1 cut(s) 1471
PsrI GAACNNNNNNTAC 2 cut(s) 1570, 1602
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 676
PsuI RGATCY 1 cut(s) 839
RsaI GTAC 8 cut(s) 154, 916, 1239, 1611, 1695, 1787, 1803, 1909
RsaNI GTAC 8 cut(s) 153, 915, 1238, 1610, 1694, 1786, 1802, 1908
RseI CAYNNNNRTG 2 cut(s) 459, 744
SacI GAGCTC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 1225
SaqAI TTAA 5 cut(s) 651, 1776, 1866, 2145, 2210
SatI GCNGC 6 cut(s) 276, 279, 282, 1196, 1317, 1593
Sau3AI GATC 2 cut(s) 839, 1918
Sau96I GGNCC 7 cut(s) 64, 94, 673, 706, 1042, 1352, 1649
ScaI AGTACT 1 cut(s) 916
SchI GAGTC 6 cut(s) 26, 502, 509, 1355, 1648, 2128
ScrFI CCNGG 4 cut(s) 93, 176, 677, 2050
SduI GDGCHC 2 cut(s) 11, 2076
SfaNI GCATC 3 cut(s) 1060, 1072, 1328
SfcI CTRYAG 4 cut(s) 279, 1077, 1098, 1885
SfoI GGCGCC 1 cut(s) 71
Sfr274I CTCGAG 1 cut(s) 1471
SinI GGWCC 6 cut(s) 64, 673, 706, 1042, 1352, 1649
SlaI CTCGAG 1 cut(s) 1471
SmiMI CAYNNNNRTG 2 cut(s) 459, 744
SmlI CTYRAG 6 cut(s) 344, 826, 843, 1438, 1471, 1769
SmoI CTYRAG 6 cut(s) 344, 826, 843, 1438, 1471, 1769
SseBI AGGCCT 1 cut(s) 1264
SsiI CCGC 5 cut(s) 240, 1195, 1584, 1634, 1729
SspDI GGCGCC 1 cut(s) 69
SspMI CTAG 1 cut(s) 1293
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 1264
StyD4I CCNGG 4 cut(s) 91, 174, 675, 2048
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 6 cut(s) 746, 1102, 1529, 1609, 1864, 1899
TaiI ACGT 3 cut(s) 452, 1491, 1551
TaqI TCGA 3 cut(s) 1297, 1472, 1491
TaqII GACCGA 2 cut(s) 694, 1666
TatI WGTACW 3 cut(s) 914, 1237, 1609
TauI GCSGC 1 cut(s) 1198
TfiI GAWTC 6 cut(s) 437, 623, 1117, 1182, 1718, 1974
Tru1I TTAA 5 cut(s) 651, 1776, 1866, 2145, 2210
Tru9I TTAA 5 cut(s) 651, 1776, 1866, 2145, 2210
TscAI CASTG 4 cut(s) 309, 1812, 2070, 2181
TseFI GTSAC 6 cut(s) 413, 944, 1544, 1662, 1850, 1889
TseI GCWGC 5 cut(s) 275, 278, 281, 1316, 1592
Tsp45I GTSAC 6 cut(s) 413, 944, 1544, 1662, 1850, 1889
TspGWI ACGGA 3 cut(s) 331, 1065, 1925
TspRI CASTG 4 cut(s) 309, 1812, 2070, 2181
VpaK11BI GGWCC 6 cut(s) 64, 673, 706, 1042, 1352, 1649
XapI RAATTY 5 cut(s) 508, 814, 863, 958, 1137
XceI RCATGY 2 cut(s) 784, 1802
XcmI CCANNNNNNNNNTGG 1 cut(s) 1510
XhoI CTCGAG 1 cut(s) 1471
XmnI GAANNNNTTC 2 cut(s) 720, 732
XspI CTAG 1 cut(s) 1293
ZrmI AGTACT 1 cut(s) 916
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.