RLG00000020490

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001741
Physical Location & Seq
Forward (+)
65552339 .. 65553289
951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020490

Sequence Viewer

Length: 951 bp
ATGACTTCCCGGTCATCTCCGACTCGTAAAAGTACACACAAGATTAAACTTAGTAACAGTTTGTCCATGGACCAGAGTTCCGAAACCCAACCTCTTCCTCCGCGTCCCAGCTCGGCTCCGCGGGGACCGGAGTCACTGCCGATCAAGTTCTCCGAGTTAGAGCCCGACAAAAAACCGATTAGAAGCGGAGCCGGAGGCACGGTGTACAAAGCCGTTCACAAACCCACCGGCCGGCTCTACGCGGTGAAAGTGATCGACGGCATCGACTCCCGTGACTACTCGGTCCCGCGTCAGATCATCCAAGAGTTCCAAGTCTTCCGCGACGCTGCTAACCCTAACATCGTCAAGTGCTACGACATGTTCGACCACAACGACGAGATCCACGTCCTCCTAGAATACATGGACGGAGGTTCATTGAAAGGCAAGTACATCAACAACGAGAAAGCTCTCTCCGACTTGGCTAGGCAGATTCTCACCGGCTTGACTTTTCTCCACGGACGGCACGTAGCCCACATGGATATCAAGCCCTCGAATCTCTTGATCAACTCCAGGCATGAAGTCAAGATCGCAGATTTTGTTGGGAGAGTTTTGGCCAAGACTATGGATCCGTGTAACTCTTCTGTGGGGAACATAGCATATATGTGCCCTGAGAGAATCAATACCGATCTAAATCACGGAAAGTTTGACGCCTGCACCGGCGATATATGGAGCGTCGGAGTCAGCATCTTGGAGTTTTACATAGGGCGGTATCCCTTCTCTGCGCACAAGGGAGATTGGAGCTTGATGTGGGGCATATGTATGTCTCAGCCTCCTGAAGCTCCTATATATGCGTCAAGAGAGTTGAGGCATTTCATTGCTTGCTGTTTGCAGAGAGAGCCGAAAAAGAGACTGTCGGCGGCGCAGTTGTTGCGGCACCCATTTATCTCCGGCAACAGCAGCGGACAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

317

Amino Acids

35.34

Weight (kDa)

9.15

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 58 - 304 1.7e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 59 - 308 1.9e-54 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 10, 281
Acc16I TGCGCA 1 cut(s) 762
AccB1I GGYRCC 1 cut(s) 912
AccII CGCG 5 cut(s) 103, 121, 242, 289, 321
AclWI GGATC 3 cut(s) 373, 599, 612
AcoI YGGCCR 2 cut(s) 229, 591
AcuI CTGAAG 1 cut(s) 834
AcyI GRCGYC 1 cut(s) 687
AfaI GTAC 3 cut(s) 34, 206, 428
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 1 cut(s) 418
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 1 cut(s) 548
AluBI AGCT 4 cut(s) 111, 446, 780, 818
AluI AGCT 4 cut(s) 111, 446, 780, 818
Alw26I GTCTC 2 cut(s) 807, 880
AlwI GGATC 3 cut(s) 373, 599, 612
AoxI GGCC 2 cut(s) 229, 591
ApeKI GCWGC 2 cut(s) 326, 936
AspLEI GCGC 2 cut(s) 763, 901
AspS9I GGNCC 3 cut(s) 70, 125, 283
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 256, 466
AvaII GGWCC 3 cut(s) 70, 125, 283
BaeGI GKGCMC 1 cut(s) 647
BalI TGGCCA 1 cut(s) 593
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 912
BanII GRGCYC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 313
BceAI ACGGC 3 cut(s) 197, 274, 515
BciT130I CCWGG 1 cut(s) 550
BciVI GTATCC 1 cut(s) 759
BclI TGATCA 1 cut(s) 540
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 807, 880
BfaI CTAG 2 cut(s) 392, 462
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 4 cut(s) 327, 897, 911, 937
BlsI GCNGC 4 cut(s) 328, 898, 912, 938
Bme1390I CCNGG 2 cut(s) 10, 550
Bme18I GGWCC 3 cut(s) 70, 125, 283
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 3 cut(s) 70, 125, 283
BmiI GGNNCC 6 cut(s) 117, 126, 190, 285, 606, 914
BmrFI CCNGG 2 cut(s) 10, 550
BmsI GCATC 2 cut(s) 270, 732
BoxI GACNNNNGTC 1 cut(s) 130
BpiI GAAGAC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 532
BpuMI CCSGG 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 505
BsaBI GATNNNNATC 1 cut(s) 669
BsaHI GRCGYC 1 cut(s) 687
BsaJI CCNNGG 3 cut(s) 66, 119, 493
BsaWI WCCGGW 1 cut(s) 127
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 4 cut(s) 227, 231, 476, 695
Bse3DI GCAATG 1 cut(s) 852
Bse8I GATNNNNATC 1 cut(s) 669
BseBI CCWGG 1 cut(s) 550
BseDI CCNNGG 3 cut(s) 66, 119, 493
BseGI GGATG 1 cut(s) 297
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 639, 818
BseSI GKGCMC 1 cut(s) 647
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 1 cut(s) 313
BseYI CCCAGC 1 cut(s) 107
BsgI GTGCAG 1 cut(s) 676
Bsh1236I CGCG 5 cut(s) 103, 121, 242, 289, 321
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 2 cut(s) 231, 593
BshNI GGYRCC 1 cut(s) 912
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 8 cut(s) 10, 128, 192, 228, 232, 477, 696, 927
BslFI GGGAC 3 cut(s) 90, 138, 269
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 2 cut(s) 807, 880
BsmFI GGGAC 3 cut(s) 90, 138, 269
BsnI GGCC 2 cut(s) 231, 593
Bsp1286I GDGCHC 2 cut(s) 165, 647
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
Bsp19I CCATGG 1 cut(s) 66
BspANI GGCC 2 cut(s) 231, 593
BspCNI CTCAG 2 cut(s) 640, 817
BspFNI CGCG 5 cut(s) 103, 121, 242, 289, 321
BspLI GGNNCC 6 cut(s) 117, 126, 190, 285, 606, 914
BspPI GGATC 3 cut(s) 373, 599, 612
BspT107I GGYRCC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 4 cut(s) 227, 231, 476, 695
BsrGI TGTACA 1 cut(s) 204
BssAI RCCGGY 4 cut(s) 227, 231, 476, 695
BssECI CCNNGG 3 cut(s) 66, 119, 493
BssMI GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
BssNI GRCGYC 1 cut(s) 687
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 550
Bst4CI ACNGT 3 cut(s) 59, 202, 891
Bst6I CTCTTC 2 cut(s) 99, 622
BstACI GRCGYC 1 cut(s) 687
BstAPI GCANNNNNTGC 1 cut(s) 907
BstAUI TGTACA 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 505
BstC8I GCNNGC 3 cut(s) 233, 691, 859
BstDEI CTNAG 3 cut(s) 50, 648, 804
BstDSI CCRYGG 3 cut(s) 66, 119, 493
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 5 cut(s) 103, 121, 242, 289, 321
BstHHI GCGC 2 cut(s) 763, 901
BstKTI GATC 8 cut(s) 144, 255, 297, 381, 543, 567, 607, 667
BstMAI GTCTC 2 cut(s) 807, 880
BstMBI GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 874, 907, 936
BstNI CCWGG 1 cut(s) 550
BstNSI RCATGY 1 cut(s) 361
BstPAI GACNNNNGTC 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 8, 548
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 5 cut(s) 103, 121, 242, 289, 321
BstV1I GCAGC 1 cut(s) 313
BstV2I GAAGAC 1 cut(s) 307
BstX2I RGATCY 2 cut(s) 378, 604
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 2 cut(s) 378, 604
BstZI CGGCCG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 2 cut(s) 231, 593
BtgI CCRYGG 3 cut(s) 66, 119, 493
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 297
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 233, 691, 859
CfoI GCGC 2 cut(s) 763, 901
Cfr10I RCCGGY 4 cut(s) 227, 231, 476, 695
Cfr13I GGNCC 3 cut(s) 70, 125, 283
Cfr42I CCGCGG 1 cut(s) 122
CseI GACGC 6 cut(s) 92, 278, 332, 695, 700, 819
Csp6I GTAC 3 cut(s) 33, 205, 427
CviAII CATG 5 cut(s) 67, 358, 400, 514, 554
CviQI GTAC 3 cut(s) 33, 205, 427
DdeI CTNAG 3 cut(s) 50, 648, 804
DpnI GATC 8 cut(s) 143, 254, 296, 380, 542, 566, 606, 666
DpnII GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
DrdI GACNNNNNNGTC 2 cut(s) 10, 281
DseDI GACNNNNNNGTC 2 cut(s) 10, 281
EaeI YGGCCR 2 cut(s) 229, 591
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 99, 622
EarI CTCTTC 2 cut(s) 99, 622
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 165
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 3 cut(s) 70, 125, 283
Eco52I CGGCCG 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 834
EcoRII CCWGG 1 cut(s) 548
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 5 cut(s) 70, 361, 403, 517, 557
FaqI GGGAC 3 cut(s) 90, 138, 269
FatI CATG 5 cut(s) 66, 357, 399, 513, 553
FauI CCCGC 2 cut(s) 114, 294
FauNDI CATATG 1 cut(s) 794
FbaI TGATCA 1 cut(s) 540
Fnu4HI GCNGC 4 cut(s) 327, 897, 911, 937
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 4 cut(s) 327, 897, 911, 937
FspBI CTAG 2 cut(s) 392, 462
FspI TGCGCA 1 cut(s) 762
GlaI GCGC 2 cut(s) 762, 900
GluI GCNGC 4 cut(s) 327, 897, 911, 937
GsaI CCCAGC 1 cut(s) 111
GsuI CTGGAG 1 cut(s) 532
HaeIII GGCC 2 cut(s) 231, 593
HapII CCGG 8 cut(s) 10, 128, 192, 228, 232, 477, 696, 927
HgaI GACGC 6 cut(s) 92, 278, 332, 695, 700, 819
HhaI GCGC 2 cut(s) 763, 901
Hin1I GRCGYC 1 cut(s) 687
Hin1II CATG 5 cut(s) 70, 361, 403, 517, 557
Hin6I GCGC 2 cut(s) 761, 899
HinP1I GCGC 2 cut(s) 761, 899
HinfI GANTC 7 cut(s) 22, 131, 266, 469, 532, 654, 717
HpaII CCGG 8 cut(s) 10, 128, 192, 228, 232, 477, 696, 927
HphI GGTGA 2 cut(s) 256, 466
Hpy166II GTNNAC 3 cut(s) 35, 205, 217
Hpy188I TCNGA 6 cut(s) 21, 82, 154, 294, 454, 716
Hpy188III TCNNGA 4 cut(s) 538, 562, 812, 834
Hpy8I GTNNAC 3 cut(s) 35, 205, 217
Hpy99I CGWCG 4 cut(s) 260, 326, 377, 716
HpyAV CCTTC 1 cut(s) 763
HpyCH4III ACNGT 3 cut(s) 59, 202, 891
HpyCH4IV ACGT 2 cut(s) 384, 504
HpyCH4V TGCA 2 cut(s) 693, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 874, 907, 936
HpyF3I CTNAG 3 cut(s) 50, 648, 804
HpySE526I ACGT 2 cut(s) 384, 504
Hsp92I GRCGYC 1 cut(s) 687
Hsp92II CATG 5 cut(s) 70, 361, 403, 517, 557
HspAI GCGC 2 cut(s) 761, 899
KroI GCCGGC 1 cut(s) 231
KroNI GCCGGC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 540
KspI CCGCGG 1 cut(s) 122
Kzo9I GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
LmnI GCTCC 5 cut(s) 121, 188, 708, 777, 823
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 2 cut(s) 270, 732
MaeI CTAG 2 cut(s) 392, 462
MaeII ACGT 2 cut(s) 384, 504
MaeIII GTNAC 4 cut(s) 53, 132, 272, 611
MalI GATC 8 cut(s) 143, 254, 296, 380, 542, 566, 606, 666
MboI GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
MboII GAAGA 3 cut(s) 86, 307, 609
MflI RGATCY 2 cut(s) 378, 604
MhlI GDGCHC 2 cut(s) 165, 647
MlsI TGGCCA 1 cut(s) 593
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 4 cut(s) 16, 140, 260, 726
MmeI TCCRAC 3 cut(s) 44, 477, 694
MnlI CCTC 8 cut(s) 102, 108, 188, 398, 401, 538, 819, 837
Mox20I TGGCCA 1 cut(s) 593
MroNI GCCGGC 1 cut(s) 231
MscI TGGCCA 1 cut(s) 593
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 2 cut(s) 640, 797
Msp20I TGGCCA 1 cut(s) 593
MspA1I CMGCKG 2 cut(s) 121, 939
MspI CCGG 8 cut(s) 10, 128, 192, 228, 232, 477, 696, 927
MspR9I CCNGG 2 cut(s) 10, 550
MvaI CCWGG 1 cut(s) 550
MvnI CGCG 5 cut(s) 103, 121, 242, 289, 321
MwoI GCNNNNNNNGC 3 cut(s) 874, 907, 936
NaeI GCCGGC 1 cut(s) 233
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 794
NdeII GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
NgoMIV GCCGGC 1 cut(s) 231
NlaIII CATG 5 cut(s) 70, 361, 403, 517, 557
NlaIV GGNNCC 6 cut(s) 117, 126, 190, 285, 606, 914
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 2 cut(s) 132, 272
NsbI TGCGCA 1 cut(s) 762
NspI RCATGY 1 cut(s) 361
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 5 cut(s) 261, 360, 369, 381, 501
PdiI GCCGGC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 469, 532, 654
PkrI GCNGC 4 cut(s) 328, 898, 912, 938
PleI GAGTC 4 cut(s) 16, 139, 260, 725
PpsI GAGTC 4 cut(s) 16, 139, 260, 725
Ppu21I YACGTR 1 cut(s) 505
PscI ACATGT 1 cut(s) 357
PshAI GACNNNNGTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 548
PspFI CCCAGC 1 cut(s) 107
PspGI CCWGG 1 cut(s) 548
PspN4I GGNNCC 6 cut(s) 117, 126, 190, 285, 606, 914
PspPI GGNCC 3 cut(s) 70, 125, 283
PsuI RGATCY 2 cut(s) 378, 604
RsaI GTAC 3 cut(s) 34, 206, 428
RsaNI GTAC 3 cut(s) 33, 205, 427
RseI CAYNNNNRTG 2 cut(s) 640, 797
SacII CCGCGG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 4 cut(s) 327, 897, 911, 937
Sau3AI GATC 8 cut(s) 141, 252, 294, 378, 540, 564, 604, 664
Sau96I GGNCC 3 cut(s) 70, 125, 283
SchI GAGTC 4 cut(s) 16, 140, 260, 726
ScrFI CCNGG 2 cut(s) 10, 550
SduI GDGCHC 2 cut(s) 165, 647
SetI ASST 8 cut(s) 94, 113, 387, 412, 448, 507, 782, 820
SfaNI GCATC 2 cut(s) 270, 732
Sfr303I CCGCGG 1 cut(s) 122
SgrAI CRCCGGYG 1 cut(s) 695
SgrBI CCGCGG 1 cut(s) 122
SinI GGWCC 3 cut(s) 70, 125, 283
SmiMI CAYNNNNRTG 2 cut(s) 640, 797
SspMI CTAG 2 cut(s) 392, 462
StyD4I CCNGG 2 cut(s) 8, 548
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 3 cut(s) 59, 202, 891
TaiI ACGT 2 cut(s) 387, 507
TaqI TCGA 4 cut(s) 255, 264, 363, 530
TaqII GACCGA 1 cut(s) 271
TatI WGTACW 3 cut(s) 32, 204, 426
TauI GCSGC 2 cut(s) 899, 913
TfiI GAWTC 3 cut(s) 469, 532, 654
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 141
TseFI GTSAC 2 cut(s) 132, 272
TseI GCWGC 2 cut(s) 326, 936
Tsp45I GTSAC 2 cut(s) 132, 272
TspDTI ATGAA 3 cut(s) 402, 570, 841
TspGWI ACGGA 4 cut(s) 420, 510, 597, 690
TspRI CASTG 1 cut(s) 141
VpaK11BI GGWCC 3 cut(s) 70, 125, 283
XceI RCATGY 1 cut(s) 361
XspI CTAG 2 cut(s) 392, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.