AT1G51660

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001741
Physical Location & Seq
Forward (+)
19154323 .. 19156338
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G51660.1

Sequence Viewer

Length: 1101 bp
ATGAGACCGATTCAATCGCCTCCAGGAGTTTCCGTTCCGGTGAAAAGCCGTCCCCGTCGCCGTCCTGATCTTACCTTACCGCTTCCTCAACGCGATGTTTCTCTCGCTGTACCTCTTCCTCTCCCACCTACTTCCGGTGGTTCCGGTGGCTCTAGTGGATCTGCGCCGTCTTCTGGTGGTTCGGCGTCTTCAACGAACACTAACAGCTCCATAGAAGCGAAGAACTATTCGGATTTAGTGAGAGGTAACCGTATCGGAAGCGGAGCAGGTGGAACGGTATACAAAGTGATTCACCGTCCGAGTTCTCGTCTATATGCACTTAAGGTGATATACGGTAACCACGAGGAGACTGTGAGACGTCAGATCTGTAGAGAGATCGAGATTTTACGAGATGTGAATCATCCAAACGTTGTGAAATGTCACGAGATGTTTGATCAGAACGGTGAGATCCAGGTTTTGCTTGAGTTTATGGATAAAGGTTCTTTAGAAGGTGCTCATGTGTGGAAAGAGCAACAATTAGCTGATCTATCTCGTCAGATTCTTAGTGGTTTAGCTTATCTCCATAGCCGTCACATAGTTCATCGTGATATCAAACCATCGAATCTTTTGATAAACTCTGCTAAAAACGTTAAGATTGCTGATTTTGGAGTTAGTAGGATCTTGGCTCAGACTATGGATCCGTGTAATTCATCTGTTGGAACCATTGCTTATATGAGTCCTGAGAGGATTAACACTGATTTGAATCAGGGAAAGTATGATGGTTATGCTGGAGATATTTGGAGCTTAGGTGTTAGCATTTTGGAGTTTTACTTGGGGAGGTTTCCTTTCCCTGTGAGTAGACAAGGTGATTGGGCTAGTCTTATGTGTGCCATTTGTATGTCTCAGCCTCCAGAAGCTCCAGCGACTGCGTCGCCGGAGTTTCGGCATTTTATCTCGTGTTGCTTGCAGAGAGAACCGGGGAAAAGGAGGAGTGCTATGCAGCTATTGCAGCATCCTTTCATATTAAGAGCAAGTCCGAGCCAGAACAGGTCTCCTCAGAATCTACATCAACTCTTGCCTCCTCCTCGTCCTCTGTCCTCGTCTTCTTCTCCAACCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

366

Amino Acids

40.12

Weight (kDa)

9.46

Isoelectric Point (pI)

73.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 82 - 334 1.6e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 82 - 330 1.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 257
AatII GACGTC 1 cut(s) 361
Acc36I ACCTGC 1 cut(s) 257
AccI GTMKAC 2 cut(s) 279, 838
AccII CGCG 1 cut(s) 93
AciI CCGC 2 cut(s) 80, 261
AclI AACGTT 2 cut(s) 408, 627
AclWI GGATC 5 cut(s) 166, 442, 665, 671, 684
AcyI GRCGYC 2 cut(s) 185, 358
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 2 cut(s) 134, 173
AflII CTTAAG 1 cut(s) 320
AgsI TTSAA 3 cut(s) 14, 192, 742
AjnI CCWGG 2 cut(s) 22, 450
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 6 cut(s) 207, 521, 554, 783, 896, 982
AluI AGCT 6 cut(s) 207, 521, 554, 783, 896, 982
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 4 cut(s) 341, 349, 885, 1035
AlwI GGATC 5 cut(s) 166, 442, 665, 671, 684
AlwNI CAGNNNCTG 1 cut(s) 905
ApeKI GCWGC 2 cut(s) 979, 988
AspLEI GCGC 1 cut(s) 166
AsuC2I CCSGG 1 cut(s) 957
AsuHPI GGTGA 5 cut(s) 52, 284, 337, 455, 857
BamHI GGATCC 1 cut(s) 676
BauI CACGAG 3 cut(s) 341, 422, 934
BbsI GAAGAC 3 cut(s) 162, 180, 1074
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 2 cut(s) 991, 1000
BccI CCATC 2 cut(s) 604, 752
BceAI ACGGC 4 cut(s) 33, 45, 151, 552
BciT130I CCWGG 2 cut(s) 24, 452
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 957
BcoDI GTCTC 4 cut(s) 341, 349, 885, 1035
BfaI CTAG 2 cut(s) 153, 855
BfmI CTRYAG 1 cut(s) 367
BfrI CTTAAG 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 257
BglII AGATCT 1 cut(s) 363
BisI GCNGC 2 cut(s) 980, 989
BlsI GCNGC 2 cut(s) 981, 990
Bme1390I CCNGG 3 cut(s) 24, 452, 957
BmiI GGNNCC 3 cut(s) 142, 678, 700
BmrFI CCNGG 3 cut(s) 24, 452, 957
BmsI GCATC 1 cut(s) 1000
BpiI GAAGAC 3 cut(s) 162, 180, 1074
BpmI CTGGAG 4 cut(s) 6, 789, 873, 882
Bpu10I CCTNAGC 1 cut(s) 784
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 957
BsaBI GATNNNNATC 2 cut(s) 396, 741
BsaHI GRCGYC 2 cut(s) 185, 358
BsaI GGTCTC 1 cut(s) 1035
BsaJI CCNNGG 1 cut(s) 956
BsaWI WCCGGW 3 cut(s) 37, 134, 143
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 772, 802
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 173
Bse3DI GCAATG 1 cut(s) 702
Bse8I GATNNNNATC 2 cut(s) 396, 741
BseBI CCWGG 2 cut(s) 24, 452
BseDI CCNNGG 1 cut(s) 956
BseGI GGATG 2 cut(s) 400, 991
BseJI GATNNNNATC 2 cut(s) 396, 741
BseLI CCNNNNNNNGG 2 cut(s) 134, 173
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 4 cut(s) 680, 711, 896, 1049
BseRI GAGGAG 5 cut(s) 359, 982, 1023, 1050, 1053
BseXI GCAGC 2 cut(s) 991, 1000
Bsh1236I CGCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 5 cut(s) 38, 135, 144, 914, 956
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 134, 173
BsmAI GTCTC 4 cut(s) 341, 349, 885, 1035
BsmBI CGTCTC 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 36
Bso31I GGTCTC 1 cut(s) 1035
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
BspACI CCGC 2 cut(s) 80, 261
BspCNI CTCAG 4 cut(s) 679, 712, 895, 1048
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 142, 678, 700
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 5 cut(s) 166, 442, 665, 671, 684
BspTI CTTAAG 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 1035
BsrDI GCAATG 1 cut(s) 702
BssECI CCNNGG 1 cut(s) 956
BssMI GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
BssNAI GTATAC 1 cut(s) 280
BssNI GRCGYC 2 cut(s) 185, 358
BssSI CACGAG 3 cut(s) 341, 422, 934
Bst1107I GTATAC 1 cut(s) 280
Bst2BI CACGAG 3 cut(s) 341, 422, 934
Bst2UI CCWGG 2 cut(s) 24, 452
Bst4CI ACNGT 6 cut(s) 251, 277, 296, 335, 352, 443
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 2 cut(s) 185, 358
BstAFI CTTAAG 1 cut(s) 320
BstAPI GCANNNNNTGC 1 cut(s) 985
BstC8I GCNNGC 1 cut(s) 944
BstDEI CTNAG 6 cut(s) 542, 666, 720, 784, 882, 1035
BstEII GGTNACC 2 cut(s) 245, 335
BstF5I GGATG 2 cut(s) 400, 991
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 9 cut(s) 70, 161, 366, 378, 436, 450, 526, 660, 679
BstMAI GTCTC 4 cut(s) 341, 349, 885, 1035
BstMBI GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
BstMWI GCNNNNNNNGC 2 cut(s) 985, 988
BstNI CCWGG 2 cut(s) 24, 452
BstPI GGTNACC 2 cut(s) 245, 335
BstSCI CCNGG 3 cut(s) 22, 450, 955
BstSFI CTRYAG 1 cut(s) 367
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 991, 1000
BstV2I GAAGAC 3 cut(s) 162, 180, 1074
BstX2I RGATCY 5 cut(s) 158, 363, 447, 657, 676
BstYI RGATCY 5 cut(s) 158, 363, 447, 657, 676
BstZ17I GTATAC 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 2 cut(s) 400, 991
BtsIMutI CAGTG 1 cut(s) 732
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 944
CaiI CAGNNNCTG 1 cut(s) 905
CfoI GCGC 1 cut(s) 166
CseI GACGC 2 cut(s) 174, 897
Csp6I GTAC 1 cut(s) 110
CviAII CATG 1 cut(s) 497
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 6 cut(s) 542, 666, 720, 784, 882, 1035
DpnI GATC 9 cut(s) 69, 160, 365, 377, 435, 449, 525, 659, 678
DpnII GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 1035
Eco32I GATATC 1 cut(s) 589
Eco91I GGTNACC 2 cut(s) 245, 335
EcoO65I GGTNACC 2 cut(s) 245, 335
EcoRII CCWGG 2 cut(s) 22, 450
EcoRV GATATC 1 cut(s) 589
Esp3I CGTCTC 1 cut(s) 349
FaeI CATG 1 cut(s) 500
FaqI GGGAC 1 cut(s) 36
FatI CATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 2 cut(s) 279, 838
Fnu4HI GCNGC 2 cut(s) 980, 989
FokI GGATG 2 cut(s) 387, 978
Fsp4HI GCNGC 2 cut(s) 980, 989
FspBI CTAG 2 cut(s) 153, 855
GlaI GCGC 1 cut(s) 165
GluI GCNGC 2 cut(s) 980, 989
GsuI CTGGAG 4 cut(s) 6, 789, 873, 882
HapII CCGG 5 cut(s) 38, 135, 144, 914, 956
HgaI GACGC 2 cut(s) 174, 897
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 2 cut(s) 185, 358
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 8 cut(s) 10, 289, 397, 538, 601, 715, 742, 1039
HpaII CCGG 5 cut(s) 38, 135, 144, 914, 956
HphI GGTGA 5 cut(s) 52, 284, 337, 455, 857
Hpy166II GTNNAC 2 cut(s) 280, 839
Hpy188I TCNGA 9 cut(s) 232, 257, 300, 363, 438, 537, 669, 1017, 1038
Hpy188III TCNNGA 6 cut(s) 65, 379, 422, 584, 719, 890
Hpy8I GTNNAC 2 cut(s) 280, 839
Hpy99I CGWCG 2 cut(s) 60, 913
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 6 cut(s) 251, 277, 296, 335, 352, 443
HpyCH4IV ACGT 3 cut(s) 358, 408, 627
HpyCH4V TGCA 4 cut(s) 317, 946, 979, 988
HpyF10VI GCNNNNNNNGC 2 cut(s) 985, 988
HpyF3I CTNAG 6 cut(s) 542, 666, 720, 784, 882, 1035
HpySE526I ACGT 3 cut(s) 358, 408, 627
Hsp92I GRCGYC 2 cut(s) 185, 358
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 1 cut(s) 164
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
LmnI GCTCC 4 cut(s) 212, 263, 780, 901
Lsp1109I GCAGC 2 cut(s) 991, 1000
LweI GCATC 1 cut(s) 1000
MaeI CTAG 2 cut(s) 153, 855
MaeII ACGT 3 cut(s) 358, 408, 627
MaeIII GTNAC 4 cut(s) 245, 335, 419, 569
MalI GATC 9 cut(s) 69, 160, 365, 377, 435, 449, 525, 659, 678
MboI GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
MboII GAAGA 6 cut(s) 107, 162, 180, 232, 1074, 1077
MflI RGATCY 5 cut(s) 158, 363, 447, 657, 676
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 2 cut(s) 515, 685
MlyI GAGTC 1 cut(s) 724
MmeI TCCRAC 1 cut(s) 676
MseI TTAA 4 cut(s) 321, 630, 729, 1004
MslI CAYNNNNRTG 1 cut(s) 875
MspCI CTTAAG 1 cut(s) 320
MspI CCGG 5 cut(s) 38, 135, 144, 914, 956
MspR9I CCNGG 3 cut(s) 24, 452, 957
MvaI CCWGG 2 cut(s) 24, 452
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 985, 988
NciI CCSGG 1 cut(s) 957
NdeII GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 3 cut(s) 142, 678, 700
NmuCI GTSAC 2 cut(s) 419, 569
PaqCI CACCTGC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PfeI GAWTC 7 cut(s) 10, 289, 397, 538, 601, 742, 1039
PflFI GACNNNGTC 1 cut(s) 907
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 2 cut(s) 981, 990
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
Psp1406I AACGTT 2 cut(s) 408, 627
Psp6I CCWGG 2 cut(s) 22, 450
PspEI GGTNACC 2 cut(s) 245, 335
PspGI CCWGG 2 cut(s) 22, 450
PspN4I GGNNCC 3 cut(s) 142, 678, 700
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 5 cut(s) 158, 363, 447, 657, 676
PsyI GACNNNGTC 1 cut(s) 907
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
RseI CAYNNNNRTG 1 cut(s) 875
SaqAI TTAA 4 cut(s) 321, 630, 729, 1004
SatI GCNGC 2 cut(s) 980, 989
Sau3AI GATC 9 cut(s) 67, 158, 363, 375, 433, 447, 523, 657, 676
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 3 cut(s) 24, 452, 957
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 1 cut(s) 1000
SfcI CTRYAG 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 875
SmlI CTYRAG 2 cut(s) 320, 461
SmoI CTYRAG 2 cut(s) 320, 461
Sse9I AATT 2 cut(s) 515, 685
SsiI CCGC 2 cut(s) 80, 261
SspMI CTAG 2 cut(s) 153, 855
StyD4I CCNGG 3 cut(s) 22, 450, 955
TaaI ACNGT 6 cut(s) 251, 277, 296, 335, 352, 443
TaiI ACGT 3 cut(s) 361, 411, 630
TaqI TCGA 2 cut(s) 378, 599
TaqII GACCGA 1 cut(s) 22
TasI AATT 2 cut(s) 515, 685
TfiI GAWTC 7 cut(s) 10, 289, 397, 538, 601, 742, 1039
Tru1I TTAA 4 cut(s) 321, 630, 729, 1004
Tru9I TTAA 4 cut(s) 321, 630, 729, 1004
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 2 cut(s) 419, 569
TseI GCWGC 2 cut(s) 979, 988
Tsp45I GTSAC 2 cut(s) 419, 569
TspDTI ATGAA 3 cut(s) 569, 678, 988
TspGWI ACGGA 2 cut(s) 22, 669
TspRI CASTG 1 cut(s) 739
Tth111I GACNNNGTC 1 cut(s) 907
Vha464I CTTAAG 1 cut(s) 320
XmiI GTMKAC 2 cut(s) 279, 838
XspI CTAG 2 cut(s) 153, 855
ZraI GACGTC 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.