Rroxscaffold_2G00097240

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001741
Physical Location & Seq
Reverse (-)
18479771 .. 18480859
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097240.1

Sequence Viewer

Length: 1089 bp
ATGGAGGAACTTCCGATCAACTTCTTGGAGCCGGAGCGGGCGATCAACGGGATCAGAAGCGGATCATCTCGGCTTCGGGAAAGTATGTTGACAAGGTCTGGCCGATCATCTTCAACCGGTCAAAGTATACACCCGGTAAAACATGGTAAGAGTTTCTCCATGGACCAAAGTTCCGTACCCCAGCCTCTTCCTCCGGTTTCCAGCTCGGCTCTTCCGGTACCGGAGTCACTTCTGATCAACTTCTCGGATATAGAGCGGGAGAAAAAACCGATTAGAAGCGGAGCCGGAGGCACGGTCTACAAAGCCGTTCACAAGCCGACCGGCCGGCTTTACGCGGTGAAAGTGATCTACGGAGACCCTGAGGACTCGGTCCGGCGTCAGATCTTCGAAGAGTTCCAAGTCCTCCGCAAAGTTGCTAACCCTAACGTCGTCAAGTGCTACGACATTTTCGATCACAACAGCGAGATACAGGTGCTGCTAGAATTCATGGACGGAGGTTCGTTAAAGGGCAAGTACATCCACGACGAGAAAGCTCTCTCCAATTTGGCTCGGCAGATCCTCACCGGCTTGGCCTATCTCCACAGACGGCACGTCATCCACCGCGATATCAAGCCCGCTAATCTCTTGATCAACACCCGAAATGAAGTCAAAATTGCTGATTTCGTGGGAAAAGTTTTAGCGCGGACTATGGACCCTTGTAACTCTTGTGTGGGAAATATAGCCTACATGAGCCCGGAGAGGATCAACCCCGATCTCAATCAGGGCAATTACGACGGCTACGCCGGCGATATTTGGAGCTTTGGGGTCAGCATCTTGGAAATTTACGTAGGTCGGTACCCCTTCTCTGTGGATCGTTACCAAAAAGGATACTGGTTCCGCTTAACTTGGGTAATATGTATGTCTCAGCCTCCTGAAGCTCCTTCTACTGCGTCCAGAGAGTTCAGGCATTTCATTGCTTGCTGTTTGCAACGAGAGCCGAATAAAAGATTCTCGGCGGCTCAGTTGTTGCAGCACCCATTCATCTCTGGCAATGGCGGTGGACAGACCCAGGGGATCAGTAGTACGTCATGCTTGTTTTACGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

362

Amino Acids

40.68

Weight (kDa)

8.96

Isoelectric Point (pI)

58.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 88 - 337 7.9e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 89 - 341 2.8e-60 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 217, 834
AccB1I GGYRCC 2 cut(s) 217, 834
AccBSI CCGCTC 2 cut(s) 37, 256
AccI GTMKAC 2 cut(s) 127, 297
AccII CGCG 3 cut(s) 335, 603, 682
AclWI GGATC 6 cut(s) 59, 70, 550, 749, 858, 1061
AcoI YGGCCR 2 cut(s) 100, 322
AcsI RAATTY 2 cut(s) 482, 819
AcuI CTGAAG 1 cut(s) 933
AcyI GRCGYC 1 cut(s) 376
AfaI GTAC 5 cut(s) 177, 219, 515, 836, 1063
AgeI ACCGGT 1 cut(s) 116
AgsI TTSAA 1 cut(s) 114
AhdI GACNNNNNGTC 1 cut(s) 590
AjiI CACGTC 1 cut(s) 592
AjnI CCWGG 1 cut(s) 1047
AluBI AGCT 4 cut(s) 204, 533, 798, 917
AluI AGCT 4 cut(s) 204, 533, 798, 917
Alw26I GTCTC 2 cut(s) 348, 906
AlwI GGATC 6 cut(s) 59, 70, 550, 749, 858, 1061
AlwNI CAGNNNCTG 1 cut(s) 475
AoxI GGCC 3 cut(s) 100, 322, 570
ApeKI GCWGC 2 cut(s) 475, 1009
ApoI RAATTY 2 cut(s) 482, 819
AsiGI ACCGGT 1 cut(s) 116
Asp718I GGTACC 2 cut(s) 217, 834
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 3 cut(s) 163, 370, 691
AsuC2I CCSGG 2 cut(s) 134, 734
AsuHPI GGTGA 2 cut(s) 349, 553
AsuII TTCGAA 1 cut(s) 387
AvaII GGWCC 3 cut(s) 163, 370, 691
AxyI CCTNAGG 1 cut(s) 360
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BanI GGYRCC 2 cut(s) 217, 834
BanII GRGCYC 1 cut(s) 734
BbvI GCAGC 2 cut(s) 462, 1021
BceAI ACGGC 3 cut(s) 290, 602, 790
BciT130I CCWGG 1 cut(s) 1049
BciVI GTATCC 1 cut(s) 860
BclI TGATCA 2 cut(s) 234, 627
BcnI CCSGG 2 cut(s) 134, 734
BcoDI GTCTC 2 cut(s) 348, 906
BfaI CTAG 1 cut(s) 479
BfuI GTATCC 1 cut(s) 860
BglII AGATCT 1 cut(s) 381
BisI GCNGC 3 cut(s) 476, 996, 1010
BlsI GCNGC 3 cut(s) 477, 997, 1011
Bme1390I CCNGG 3 cut(s) 134, 734, 1049
Bme18I GGWCC 3 cut(s) 163, 370, 691
BmeRI GACNNNNNGTC 1 cut(s) 590
BmgBI CACGTC 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 163, 370, 691
BmiI GGNNCC 6 cut(s) 30, 219, 283, 693, 836, 875
BmrFI CCNGG 3 cut(s) 134, 734, 1049
BmsI GCATC 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 387
BpuMI CCSGG 2 cut(s) 134, 734
BsaAI YACGTR 1 cut(s) 826
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 159, 1047, 1048
BsaWI WCCGGW 4 cut(s) 116, 193, 214, 220
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bse118I RCCGGY 5 cut(s) 116, 320, 324, 563, 782
Bse1I ACTGG 1 cut(s) 875
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 2 cut(s) 951, 1036
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 1 cut(s) 1049
BseDI CCNNGG 3 cut(s) 159, 1047, 1048
BseGI GGATG 2 cut(s) 516, 594
BseJI GATNNNNATC 1 cut(s) 756
BseMI GCAATG 2 cut(s) 951, 1036
BseMII CTCAG 3 cut(s) 351, 917, 1013
BseNI ACTGG 1 cut(s) 875
BseX3I CGGCCG 1 cut(s) 322
BseXI GCAGC 2 cut(s) 462, 1021
BseYI CCCAGC 1 cut(s) 180
Bsh1236I CGCG 3 cut(s) 335, 603, 682
Bsh1285I CGRYCG 2 cut(s) 321, 325
BshFI GGCC 3 cut(s) 102, 324, 572
BshNI GGYRCC 2 cut(s) 217, 834
BshTI ACCGGT 1 cut(s) 116
BsiEI CGRYCG 2 cut(s) 321, 325
BsmAI GTCTC 2 cut(s) 348, 906
BsnI GGCC 3 cut(s) 102, 324, 572
Bso31I GGTCTC 1 cut(s) 348
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 734
Bsp19I CCATGG 1 cut(s) 159
BspANI GGCC 3 cut(s) 102, 324, 572
BspCNI CTCAG 3 cut(s) 352, 916, 1012
BspFNI CGCG 3 cut(s) 335, 603, 682
BspLI GGNNCC 6 cut(s) 30, 219, 283, 693, 836, 875
BspPI GGATC 6 cut(s) 59, 70, 550, 749, 858, 1061
BspQI GCTCTTC 1 cut(s) 216
BspT104I TTCGAA 1 cut(s) 387
BspT107I GGYRCC 2 cut(s) 217, 834
BspTNI GGTCTC 1 cut(s) 348
BsrBI CCGCTC 2 cut(s) 37, 256
BsrDI GCAATG 2 cut(s) 951, 1036
BsrFI RCCGGY 5 cut(s) 116, 320, 324, 563, 782
BsrI ACTGG 1 cut(s) 875
BssAI RCCGGY 5 cut(s) 116, 320, 324, 563, 782
BssECI CCNNGG 3 cut(s) 159, 1047, 1048
BssNAI GTATAC 1 cut(s) 128
BssNI GRCGYC 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 159
Bst1107I GTATAC 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 1049
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 3 cut(s) 192, 216, 384
BstACI GRCGYC 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 826
BstBI TTCGAA 1 cut(s) 387
BstC8I GCNNGC 5 cut(s) 39, 326, 615, 784, 958
BstDEI CTNAG 3 cut(s) 360, 903, 999
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 2 cut(s) 516, 594
BstFNI CGCG 3 cut(s) 335, 603, 682
BstHHI GCGC 1 cut(s) 682
BstMAI GTCTC 2 cut(s) 348, 906
BstMCI CGRYCG 2 cut(s) 321, 325
BstMWI GCNNNNNNNGC 2 cut(s) 783, 973
BstNI CCWGG 1 cut(s) 1049
BstSCI CCNGG 3 cut(s) 132, 732, 1047
BstSNI TACGTA 1 cut(s) 826
BstUI CGCG 3 cut(s) 335, 603, 682
BstV1I GCAGC 2 cut(s) 462, 1021
BstX2I RGATCY 2 cut(s) 381, 555
BstYI RGATCY 2 cut(s) 381, 555
BstZ17I GTATAC 1 cut(s) 128
BstZI CGGCCG 1 cut(s) 322
Bsu36I CCTNAGG 1 cut(s) 360
BsuI GTATCC 1 cut(s) 860
BsuRI GGCC 3 cut(s) 102, 324, 572
BtgI CCRYGG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 592
BtsCI GGATG 2 cut(s) 516, 594
Cac8I GCNNGC 5 cut(s) 39, 326, 615, 784, 958
CaiI CAGNNNCTG 1 cut(s) 475
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 5 cut(s) 116, 320, 324, 563, 782
Cfr13I GGNCC 3 cut(s) 163, 370, 691
CpoI CGGWCCG 1 cut(s) 370
CseI GACGC 2 cut(s) 365, 918
Csp6I GTAC 5 cut(s) 176, 218, 514, 835, 1062
CspAI ACCGGT 1 cut(s) 116
CspCI CAANNNNNGTGG 2 cut(s) 1018, 1053
CspI CGGWCCG 1 cut(s) 370
CviAII CATG 5 cut(s) 143, 160, 487, 727, 1068
CviQI GTAC 5 cut(s) 176, 218, 514, 835, 1062
DdeI CTNAG 3 cut(s) 360, 903, 999
DriI GACNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 2 cut(s) 100, 322
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 3 cut(s) 192, 216, 384
Eam1105I GACNNNNNGTC 1 cut(s) 590
EarI CTCTTC 3 cut(s) 192, 216, 384
EclXI CGGCCG 1 cut(s) 322
Eco105I TACGTA 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 734
Eco31I GGTCTC 1 cut(s) 348
Eco32I GATATC 1 cut(s) 607
Eco47I GGWCC 3 cut(s) 163, 370, 691
Eco52I CGGCCG 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 933
Eco81I CCTNAGG 1 cut(s) 360
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 1047
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 734
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 5 cut(s) 146, 163, 490, 730, 1071
FatI CATG 5 cut(s) 142, 159, 486, 726, 1067
FauI CCCGC 3 cut(s) 30, 249, 622
FbaI TGATCA 2 cut(s) 234, 627
FblI GTMKAC 2 cut(s) 127, 297
Fnu4HI GCNGC 3 cut(s) 476, 996, 1010
FokI GGATG 2 cut(s) 503, 581
FriOI GRGCYC 1 cut(s) 734
Fsp4HI GCNGC 3 cut(s) 476, 996, 1010
FspBI CTAG 1 cut(s) 479
GlaI GCGC 1 cut(s) 681
GluI GCNGC 3 cut(s) 476, 996, 1010
GsaI CCCAGC 1 cut(s) 184
HaeIII GGCC 3 cut(s) 102, 324, 572
HgaI GACGC 2 cut(s) 365, 918
HhaI GCGC 1 cut(s) 682
Hin1I GRCGYC 1 cut(s) 376
Hin1II CATG 5 cut(s) 146, 163, 490, 730, 1071
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HinfI GANTC 3 cut(s) 224, 365, 987
HphI GGTGA 2 cut(s) 349, 553
Hpy166II GTNNAC 5 cut(s) 90, 128, 298, 310, 1040
Hpy188I TCNGA 5 cut(s) 15, 56, 234, 247, 381
Hpy188III TCNNGA 4 cut(s) 77, 625, 911, 933
Hpy8I GTNNAC 5 cut(s) 90, 128, 298, 310, 1040
Hpy99I CGWCG 3 cut(s) 431, 527, 776
HpyAV CCTTC 2 cut(s) 850, 930
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 4 cut(s) 426, 591, 825, 1064
HpyCH4V TGCA 2 cut(s) 967, 1009
HpyF10VI GCNNNNNNNGC 2 cut(s) 783, 973
HpyF3I CTNAG 3 cut(s) 360, 903, 999
HpySE526I ACGT 4 cut(s) 426, 591, 825, 1064
Hsp92I GRCGYC 1 cut(s) 376
Hsp92II CATG 5 cut(s) 146, 163, 490, 730, 1071
HspAI GCGC 1 cut(s) 680
KpnI GGTACC 2 cut(s) 221, 838
KroI GCCGGC 2 cut(s) 324, 782
KroNI GCCGGC 2 cut(s) 326, 784
Ksp22I TGATCA 2 cut(s) 234, 627
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 5 cut(s) 28, 34, 281, 795, 922
Lsp1109I GCAGC 2 cut(s) 462, 1021
LweI GCATC 1 cut(s) 819
MaeI CTAG 1 cut(s) 479
MaeII ACGT 4 cut(s) 426, 591, 825, 1064
MaeIII GTNAC 3 cut(s) 225, 698, 854
MbiI CCGCTC 2 cut(s) 37, 256
MboII GAAGA 5 cut(s) 102, 179, 203, 376, 401
MflI RGATCY 2 cut(s) 381, 555
MhlI GDGCHC 1 cut(s) 734
MluCI AATT 5 cut(s) 482, 541, 651, 766, 819
MlyI GAGTC 2 cut(s) 233, 359
MnlI CCTC 9 cut(s) 195, 201, 281, 355, 413, 488, 569, 732, 918
MreI CGCCGGCG 1 cut(s) 782
MroNI GCCGGC 2 cut(s) 324, 782
MseI TTAA 2 cut(s) 503, 881
MspR9I CCNGG 3 cut(s) 134, 734, 1049
MvaI CCWGG 1 cut(s) 1049
MvnI CGCG 3 cut(s) 335, 603, 682
MwoI GCNNNNNNNGC 2 cut(s) 783, 973
NaeI GCCGGC 2 cut(s) 326, 784
NciI CCSGG 2 cut(s) 134, 734
NcoI CCATGG 1 cut(s) 159
NgoMIV GCCGGC 2 cut(s) 324, 782
NlaIII CATG 5 cut(s) 146, 163, 490, 730, 1071
NlaIV GGNNCC 6 cut(s) 30, 219, 283, 693, 836, 875
NmeAIII GCCGAG 4 cut(s) 49, 185, 529, 971
NmuCI GTSAC 1 cut(s) 225
NspV TTCGAA 1 cut(s) 387
PasI CCCWGGG 1 cut(s) 1048
PciSI GCTCTTC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PdiI GCCGGC 2 cut(s) 326, 784
PfeI GAWTC 1 cut(s) 987
PflFI GACNNNGTC 2 cut(s) 94, 368
PinAI ACCGGT 1 cut(s) 116
PkrI GCNGC 3 cut(s) 477, 997, 1011
PleI GAGTC 2 cut(s) 232, 359
PpsI GAGTC 2 cut(s) 232, 359
Ppu21I YACGTR 1 cut(s) 826
Psp6I CCWGG 1 cut(s) 1047
PspFI CCCAGC 1 cut(s) 180
PspGI CCWGG 1 cut(s) 1047
PspN4I GGNNCC 6 cut(s) 30, 219, 283, 693, 836, 875
PspPI GGNCC 3 cut(s) 163, 370, 691
PstNI CAGNNNCTG 1 cut(s) 475
PsuI RGATCY 2 cut(s) 381, 555
PsyI GACNNNGTC 2 cut(s) 94, 368
RsaI GTAC 5 cut(s) 177, 219, 515, 836, 1063
RsaNI GTAC 5 cut(s) 176, 218, 514, 835, 1062
Rsr2I CGGWCCG 1 cut(s) 370
RsrII CGGWCCG 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 503, 881
SatI GCNGC 3 cut(s) 476, 996, 1010
Sau96I GGNCC 3 cut(s) 163, 370, 691
SchI GAGTC 2 cut(s) 233, 359
ScrFI CCNGG 3 cut(s) 134, 734, 1049
SduI GDGCHC 1 cut(s) 734
SfaNI GCATC 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 387
SgrAI CRCCGGYG 1 cut(s) 782
SinI GGWCC 3 cut(s) 163, 370, 691
SnaBI TACGTA 1 cut(s) 826
Sse9I AATT 5 cut(s) 482, 541, 651, 766, 819
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 3 cut(s) 132, 732, 1047
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 4 cut(s) 429, 594, 828, 1067
TaqI TCGA 2 cut(s) 387, 450
TaqII GACCGA 1 cut(s) 358
TasI AATT 5 cut(s) 482, 541, 651, 766, 819
TatI WGTACW 1 cut(s) 513
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 1 cut(s) 987
Tru1I TTAA 2 cut(s) 503, 881
Tru9I TTAA 2 cut(s) 503, 881
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 2 cut(s) 475, 1009
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 4 cut(s) 475, 657, 940, 1009
TspGWI ACGGA 3 cut(s) 163, 366, 507
Tth111I GACNNNGTC 2 cut(s) 94, 368
VpaK11BI GGWCC 3 cut(s) 163, 370, 691
XapI RAATTY 2 cut(s) 482, 819
XmiI GTMKAC 2 cut(s) 127, 297
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.