Rorug02G0420700

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001741
Physical Location & Seq
Reverse (-)
54045442 .. 54046705
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0420700.1

Sequence Viewer

Length: 729 bp
ATGGGGGAAAGCAGTACTTGCTATGATAAGAACGAGGGACTGAAGAAAGGAGCCTGGGCTGCAAGTGAAGACCAGATTGTCAAAGACCACGTCAAAAAACATGGTGTGGGAAAATGGGGCAAATTATCTAGGGAAACAGGTCTGAAGAGATGTGGGAAGAGTGTGAGGCTTAGGTGGATGAATTATCTGAGGCCTGGTATCGTTAGAGGCAACATTACAGAAGATGAAGAAGAGCTCATCATCAGACTCCACAAACTCTTGGGCAACAGATGGTCACTGATAGCCGGAAGGATACCAGGGCGAACTGATAATGAAATCAAAAACTACTGGAATTCCTGCTTAAAGAGGAAGCTACAACAACAAAACCACTTGGAAGTGCCTAGAAATGAGGAGATCAGGGAGGACTTGTCATCCTCTGCTGCGGTTGCCGCTGCCGCTGAGAAGAACCCACATTTGACCGGAAACAATGTCAATGAAGACGAAGATTATCATCAGCATCATGATGTTGACTTTGGAGTTGGAATTGAGTTTGAAGCAACTGATAATAATAACGGCACTGGTGATGATGAACATCCGAGGGATGACCTTTGGTGGAACAACTTCATAATGGATCTGAATGATATCCAACTCGATGTATCTGAATTTCAATCCGACTTCCCCGAGATTTGGGAATCTAAGGTGAACGTTATCGACGTTGGAGATTACAGCACCGATCATCATGCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

242

Amino Acids

27.73

Weight (kDa)

5.22

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 16 - 63 8.1e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 19 - 77 7.4e-06 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 114 7.4e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 73 - 122 2.8e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AciI CCGC 3 cut(s) 422, 429, 435
AclI AACGTT 1 cut(s) 684
AclWI GGATC 1 cut(s) 618
AcsI RAATTY 2 cut(s) 331, 641
AcuI CTGAAG 2 cut(s) 62, 164
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 666
AgsI TTSAA 2 cut(s) 533, 647
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 53, 193, 295
AluBI AGCT 2 cut(s) 235, 352
AluI AGCT 2 cut(s) 235, 352
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 1 cut(s) 618
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 1 cut(s) 191
ApeKI GCWGC 3 cut(s) 59, 419, 431
ApoI RAATTY 2 cut(s) 331, 641
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
Asp700I GAANNNNTTC 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 572, 691
AvaI CYCGRG 1 cut(s) 659
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 75, 483
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 46, 406, 418
BccI CCATC 1 cut(s) 264
BceAI ACGGC 1 cut(s) 568
BcgI CGANNNNNNTGC 1 cut(s) 701
BciT130I CCWGG 3 cut(s) 55, 195, 297
BciVI GTATCC 1 cut(s) 285
BfaI CTAG 2 cut(s) 129, 381
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 5 cut(s) 60, 420, 429, 432, 435
BlsI GCNGC 5 cut(s) 61, 421, 430, 433, 436
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 3 cut(s) 55, 195, 297
BmeT110I CYCGRG 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 3 cut(s) 55, 195, 297
BmsI GCATC 1 cut(s) 505
BpiI GAAGAC 2 cut(s) 75, 483
Bpu10I CCTNAGC 1 cut(s) 170
BsaBI GATNNNNATC 2 cut(s) 489, 570
BsaJI CCNNGG 3 cut(s) 54, 296, 575
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 666
Bse1I ACTGG 2 cut(s) 332, 562
Bse8I GATNNNNATC 2 cut(s) 489, 570
BseBI CCWGG 3 cut(s) 55, 195, 297
BseDI CCNNGG 3 cut(s) 54, 296, 575
BseGI GGATG 4 cut(s) 183, 410, 571, 586
BseJI GATNNNNATC 2 cut(s) 489, 570
BseLI CCNNNNNNNGG 1 cut(s) 666
BseMII CTCAG 2 cut(s) 179, 429
BseNI ACTGG 2 cut(s) 332, 562
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 3 cut(s) 46, 406, 418
BshFI GGCC 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 2 cut(s) 285, 459
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 51
BsnI GGCC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 3 cut(s) 393, 610, 712
BspACI CCGC 3 cut(s) 422, 429, 435
BspANI GGCC 1 cut(s) 193
BspCNI CTCAG 2 cut(s) 180, 430
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 52
BspPI GGATC 1 cut(s) 618
BspQI GCTCTTC 1 cut(s) 225
BsrI ACTGG 2 cut(s) 332, 562
BssECI CCNNGG 3 cut(s) 54, 296, 575
BssMI GATC 3 cut(s) 393, 610, 712
Bst2UI CCWGG 3 cut(s) 55, 195, 297
Bst6I CTCTTC 3 cut(s) 140, 152, 225
BstAPI GCANNNNNTGC 1 cut(s) 18
BstDEI CTNAG 4 cut(s) 170, 188, 438, 675
BstF5I GGATG 4 cut(s) 183, 410, 571, 586
BstKTI GATC 3 cut(s) 396, 613, 715
BstMBI GATC 3 cut(s) 393, 610, 712
BstMWI GCNNNNNNNGC 5 cut(s) 18, 59, 425, 428, 434
BstNI CCWGG 3 cut(s) 55, 195, 297
BstSCI CCNGG 3 cut(s) 53, 193, 295
BstV1I GCAGC 3 cut(s) 46, 406, 418
BstV2I GAAGAC 2 cut(s) 75, 483
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 1 cut(s) 193
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 4 cut(s) 183, 410, 571, 586
BtsIMutI CAGTG 2 cut(s) 275, 555
CciI TCATGA 1 cut(s) 499
Csp6I GTAC 1 cut(s) 15
CviAII CATG 3 cut(s) 101, 500, 719
CviJI RGCY 7 cut(s) 53, 59, 169, 193, 235, 284, 352
CviKI_1 RGCY 7 cut(s) 53, 59, 169, 193, 235, 284, 352
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 4 cut(s) 170, 188, 438, 675
DpnI GATC 3 cut(s) 395, 612, 714
DpnII GATC 3 cut(s) 393, 610, 712
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 3 cut(s) 140, 152, 225
EarI CTCTTC 3 cut(s) 140, 152, 225
Ecl136II GAGCTC 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 62, 164
Eco88I CYCGRG 1 cut(s) 659
EcoICRI GAGCTC 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 3 cut(s) 53, 193, 295
EcoRV GATATC 1 cut(s) 622
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 3 cut(s) 104, 503, 722
FaiI YATR 5 cut(s) 24, 102, 501, 605, 720
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 1 cut(s) 51
FatI CATG 3 cut(s) 100, 499, 718
Fnu4HI GCNGC 5 cut(s) 60, 420, 429, 432, 435
FokI GGATG 4 cut(s) 190, 397, 558, 593
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 60, 420, 429, 432, 435
FspBI CTAG 2 cut(s) 129, 381
GluI GCNGC 5 cut(s) 60, 420, 429, 432, 435
HaeIII GGCC 1 cut(s) 193
HapII CCGG 2 cut(s) 285, 459
Hin1II CATG 3 cut(s) 104, 503, 722
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 2 cut(s) 246, 671
HpaII CCGG 2 cut(s) 285, 459
HphI GGTGA 2 cut(s) 572, 691
Hpy166II GTNNAC 2 cut(s) 508, 682
Hpy188I TCNGA 7 cut(s) 144, 189, 245, 576, 615, 640, 652
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 2 cut(s) 508, 682
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 1 cut(s) 282
HpyCH4IV ACGT 3 cut(s) 90, 684, 693
HpyCH4V TGCA 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 59, 425, 428, 434
HpyF3I CTNAG 4 cut(s) 170, 188, 438, 675
HpySE526I ACGT 3 cut(s) 90, 684, 693
Hsp92II CATG 3 cut(s) 104, 503, 722
Kzo9I GATC 3 cut(s) 393, 610, 712
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 3 cut(s) 46, 406, 418
LweI GCATC 1 cut(s) 505
MaeI CTAG 2 cut(s) 129, 381
MaeII ACGT 3 cut(s) 90, 684, 693
MaeIII GTNAC 1 cut(s) 273
MalI GATC 3 cut(s) 395, 612, 714
MboI GATC 3 cut(s) 393, 610, 712
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 5 cut(s) 122, 181, 331, 522, 641
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 4 cut(s) 499, 649, 675, 676
MnlI CCTC 9 cut(s) 28, 159, 183, 200, 339, 382, 394, 424, 570
MroXI GAANNNNTTC 1 cut(s) 599
MseI TTAA 1 cut(s) 341
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 2 cut(s) 431, 437
MspI CCGG 2 cut(s) 285, 459
MspR9I CCNGG 3 cut(s) 55, 195, 297
MvaI CCWGG 3 cut(s) 55, 195, 297
MwoI GCNNNNNNNGC 5 cut(s) 18, 59, 425, 428, 434
NdeII GATC 3 cut(s) 393, 610, 712
NlaIII CATG 3 cut(s) 104, 503, 722
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 273
PagI TCATGA 1 cut(s) 499
PceI AGGCCT 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 690
PdmI GAANNNNTTC 1 cut(s) 599
PfeI GAWTC 1 cut(s) 671
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 5 cut(s) 61, 421, 430, 433, 436
PleI GAGTC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 684
Psp6I CCWGG 3 cut(s) 53, 193, 295
PspGI CCWGG 3 cut(s) 53, 193, 295
PspN4I GGNNCC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 1 cut(s) 501
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 5 cut(s) 60, 420, 429, 432, 435
Sau3AI GATC 3 cut(s) 393, 610, 712
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 3 cut(s) 55, 195, 297
SduI GDGCHC 1 cut(s) 237
SetI ASST 9 cut(s) 93, 142, 176, 237, 354, 588, 681, 687, 696
SfaNI GCATC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 501
Sse9I AATT 5 cut(s) 122, 181, 331, 522, 641
SseBI AGGCCT 1 cut(s) 193
SsiI CCGC 3 cut(s) 422, 429, 435
SspMI CTAG 2 cut(s) 129, 381
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 193
StyD4I CCNGG 3 cut(s) 53, 193, 295
TaiI ACGT 3 cut(s) 93, 687, 696
TaqI TCGA 2 cut(s) 630, 690
TasI AATT 5 cut(s) 122, 181, 331, 522, 641
TatI WGTACW 1 cut(s) 14
TauI GCSGC 2 cut(s) 431, 437
TfiI GAWTC 1 cut(s) 671
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TscAI CASTG 2 cut(s) 282, 562
TseFI GTSAC 1 cut(s) 273
TseI GCWGC 3 cut(s) 59, 419, 431
Tsp45I GTSAC 1 cut(s) 273
TspDTI ATGAA 6 cut(s) 194, 240, 327, 489, 582, 592
TspRI CASTG 2 cut(s) 282, 562
Tth111I GACNNNGTC 1 cut(s) 89
XapI RAATTY 2 cut(s) 331, 641
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 2 cut(s) 129, 381
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.