AT3G21220

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001741
Physical Location & Seq
Forward (+)
7445604 .. 7448498
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21220.2

Sequence Viewer

Length: 1047 bp
ATGAAACCGATTCAATCTCCTTCTGGAGTAGCTTCACCTATGAAGAACCGTTTACGCAAACGTCCTGACCTAAGCTTACCACTCCCACACCGCGACGTCGCTCTCGCCGTACCTCTCCCTCTCCCACCTCCTTCTTCCTCTTCATCCGCTCCGGCGTCTTCCTCCGCGATCTCAACCAACATCTCCGCCGCTAAAAGCTTATCCGAGCTAGAACGAGTGAACCGAATCGGAAGCGGAGCCGGAGGAACGGTTTACAAAGTAATCCACACTCCGACGTCACGTCCTTTCGCTCTCAAAGTGATTTACGGAAACCACGAAGATACCGTGAGACGTCAGATCTGTAGAGAGATCGAGATCTTAAGAAGTGTTGATCATCCAAACGTTGTGAAATGTCACGATATGTTTGATCATAACGGTGAGATCCAGGTTTTGCTTGAGTTTATGGATCAAGGATCTCTTGAAGGAGCTCATATATGGCAAGAACAGGAATTAGCTGATCTCTCTCGTCAGATTCTTAGTGGATTAGCTTATCTTCATCGTCGTCATATCGTTCATCGTGATATCAAACCTTCGAATCTTCTTATAAACTCAGCTAAAAATGTGAAAATTGCTGATTTTGGTGTGAGTAGGATCTTGGCACAAACAATGGATCCTTGTAATTCATCTGTTGGTACTATTGCTTATATGAGTCCTGAGAGGATTAATACTGATTTGAATCATGGTCGTTACGATGGTTATGCTGGAGATGTTTGGAGTTTAGGTGTTAGTATCTTGGAGTTTTACTTGGGGAGGTTTCCTTTTGCTGTGAGTAGACAAGGTGATTGGGCTAGTCTTATGTGTGCTATTTGTATGTCTCAGCCACCTGAAGCTCCGGCTACGGCGTCTCAGGAGTTTCGTCACTTTGTTTCTTGTTGTTTACAGAGTGATCCTCCTAAGAGATGGTCAGCTCAACAGCTTTTGCAGCATCCTTTCATACTTAAAGCTACCGGTGGTCCTAATCTCCGTCAAATGTTGCCGCCGCCTCGTCCTCTTCCTTCTGCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

348

Amino Acids

38.33

Weight (kDa)

8.97

Isoelectric Point (pI)

69.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 71 - 325 8.7e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 72 - 321 5.2e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AatII GACGTC 3 cut(s) 99, 278, 334
AccBSI CCGCTC 1 cut(s) 149
AccI GTMKAC 1 cut(s) 811
AccII CGCG 2 cut(s) 93, 167
AciI CCGC 8 cut(s) 91, 147, 165, 186, 189, 234, 1016, 1019
AclI AACGTT 1 cut(s) 381
AclWI GGATC 7 cut(s) 415, 453, 460, 638, 644, 657, 920
AcuI CTGAAG 1 cut(s) 885
AcyI GRCGYC 5 cut(s) 96, 155, 275, 331, 881
AfaI GTAC 2 cut(s) 111, 673
AflII CTTAAG 1 cut(s) 358
AgeI ACCGGT 1 cut(s) 986
AgsI TTSAA 3 cut(s) 14, 461, 715
AhdI GACNNNNNGTC 1 cut(s) 279
AjiI CACGTC 1 cut(s) 281
AjnI CCWGG 1 cut(s) 423
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 3 cut(s) 322, 858, 888
AlwI GGATC 7 cut(s) 415, 453, 460, 638, 644, 657, 920
ApeKI GCWGC 1 cut(s) 961
AseI ATTAAT 1 cut(s) 702
AsiGI ACCGGT 1 cut(s) 986
AspS9I GGNCC 1 cut(s) 992
AsuHPI GGTGA 3 cut(s) 27, 428, 830
AsuII TTCGAA 1 cut(s) 572
AvaII GGWCC 1 cut(s) 992
BamHI GGATCC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 1 cut(s) 973
BccI CCATC 2 cut(s) 725, 933
BceAI ACGGC 2 cut(s) 92, 894
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 2 cut(s) 370, 406
BcoDI GTCTC 3 cut(s) 322, 858, 888
BfaI CTAG 2 cut(s) 209, 828
BfmI CTRYAG 1 cut(s) 340
BfrI CTTAAG 1 cut(s) 358
BglII AGATCT 2 cut(s) 336, 354
BisI GCNGC 4 cut(s) 189, 962, 1016, 1019
BlsI GCNGC 4 cut(s) 190, 963, 1017, 1020
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 1 cut(s) 992
BmeRI GACNNNNNGTC 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 992
BmiI GGNNCC 2 cut(s) 238, 651
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 973
BpiI GAAGAC 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 913, 945
BpmI CTGGAG 2 cut(s) 45, 762
Bpu10I CCTNAGC 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 455
BsaBI GATNNNNATC 2 cut(s) 353, 714
BsaHI GRCGYC 5 cut(s) 96, 155, 275, 331, 881
BsaWI WCCGGW 1 cut(s) 986
BsaXI ACNNNNNCTCC 4 cut(s) 234, 264, 745, 775
Bse118I RCCGGY 1 cut(s) 986
Bse8I GATNNNNATC 2 cut(s) 353, 714
BseBI CCWGG 1 cut(s) 425
BseGI GGATG 3 cut(s) 143, 373, 964
BseJI GATNNNNATC 2 cut(s) 353, 714
BseMII CTCAG 4 cut(s) 603, 684, 869, 899
BseXI GCAGC 1 cut(s) 973
Bsh1236I CGCG 2 cut(s) 93, 167
BshTI ACCGGT 1 cut(s) 986
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 4 cut(s) 152, 240, 872, 987
BsmAI GTCTC 3 cut(s) 322, 858, 888
BsmBI CGTCTC 2 cut(s) 322, 888
Bsp119I TTCGAA 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 469
BspACI CCGC 8 cut(s) 91, 147, 165, 186, 189, 234, 1016, 1019
BspCNI CTCAG 4 cut(s) 602, 685, 868, 898
BspFNI CGCG 2 cut(s) 93, 167
BspLI GGNNCC 2 cut(s) 238, 651
BspPI GGATC 7 cut(s) 415, 453, 460, 638, 644, 657, 920
BspT104I TTCGAA 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 358
BsrBI CCGCTC 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 986
BssAI RCCGGY 1 cut(s) 986
BssNI GRCGYC 5 cut(s) 96, 155, 275, 331, 881
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 50, 250, 325, 416
Bst6I CTCTTC 2 cut(s) 145, 1035
BstACI GRCGYC 5 cut(s) 96, 155, 275, 331, 881
BstAFI CTTAAG 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 572
BstDEI CTNAG 8 cut(s) 71, 515, 589, 693, 855, 885, 933, 1044
BstF5I GGATG 3 cut(s) 143, 373, 964
BstFNI CGCG 2 cut(s) 93, 167
BstMAI GTCTC 3 cut(s) 322, 858, 888
BstMWI GCNNNNNNNGC 1 cut(s) 961
BstNI CCWGG 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 2 cut(s) 93, 167
BstV1I GCAGC 1 cut(s) 973
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 6 cut(s) 336, 354, 420, 452, 630, 649
BstYI RGATCY 6 cut(s) 336, 354, 420, 452, 630, 649
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 3 cut(s) 143, 373, 964
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 1 cut(s) 992
CseI GACGC 2 cut(s) 144, 870
Csp6I GTAC 2 cut(s) 110, 672
CspAI ACCGGT 1 cut(s) 986
CviAII CATG 1 cut(s) 719
CviQI GTAC 2 cut(s) 110, 672
DdeI CTNAG 8 cut(s) 71, 515, 589, 693, 855, 885, 933, 1044
DriI GACNNNNNGTC 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 145, 1035
Eam1105I GACNNNNNGTC 1 cut(s) 279
EarI CTCTTC 2 cut(s) 145, 1035
EciI GGCGGA 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 467
Eco24I GRGCYC 1 cut(s) 469
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 992
Eco53kI GAGCTC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 885
EcoICRI GAGCTC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 423
EcoRV GATATC 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 469
Esp3I CGTCTC 2 cut(s) 322, 888
FaeI CATG 1 cut(s) 722
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FatI CATG 1 cut(s) 718
FbaI TGATCA 2 cut(s) 370, 406
FblI GTMKAC 1 cut(s) 811
Fnu4HI GCNGC 4 cut(s) 189, 962, 1016, 1019
FokI GGATG 3 cut(s) 130, 360, 951
FriOI GRGCYC 1 cut(s) 469
Fsp4HI GCNGC 4 cut(s) 189, 962, 1016, 1019
FspBI CTAG 2 cut(s) 209, 828
GluI GCNGC 4 cut(s) 189, 962, 1016, 1019
GsuI CTGGAG 2 cut(s) 45, 762
HapII CCGG 4 cut(s) 152, 240, 872, 987
HgaI GACGC 2 cut(s) 144, 870
Hin1I GRCGYC 5 cut(s) 96, 155, 275, 331, 881
Hin1II CATG 1 cut(s) 722
HindIII AAGCTT 2 cut(s) 73, 196
HinfI GANTC 6 cut(s) 10, 225, 511, 574, 688, 715
HpaII CCGG 4 cut(s) 152, 240, 872, 987
HphI GGTGA 3 cut(s) 27, 428, 830
Hpy166II GTNNAC 5 cut(s) 53, 220, 253, 812, 917
Hpy188I TCNGA 5 cut(s) 205, 230, 273, 336, 510
Hpy188III TCNNGA 8 cut(s) 24, 65, 352, 395, 458, 557, 692, 887
Hpy8I GTNNAC 5 cut(s) 53, 220, 253, 812, 917
Hpy99I CGWCG 4 cut(s) 98, 101, 277, 543
HpyAV CCTTC 5 cut(s) 30, 141, 455, 579, 1044
HpyCH4III ACNGT 4 cut(s) 50, 250, 325, 416
HpyCH4IV ACGT 6 cut(s) 61, 96, 275, 280, 331, 381
HpyCH4V TGCA 1 cut(s) 961
HpyF10VI GCNNNNNNNGC 1 cut(s) 961
HpyF3I CTNAG 8 cut(s) 71, 515, 589, 693, 855, 885, 933, 1044
HpySE526I ACGT 6 cut(s) 61, 96, 275, 280, 331, 381
Hsp92I GRCGYC 5 cut(s) 96, 155, 275, 331, 881
Hsp92II CATG 1 cut(s) 722
Ksp22I TGATCA 2 cut(s) 370, 406
LmnI GCTCC 4 cut(s) 154, 236, 464, 874
Lsp1109I GCAGC 1 cut(s) 973
LweI GCATC 1 cut(s) 973
MaeI CTAG 2 cut(s) 209, 828
MaeII ACGT 6 cut(s) 61, 96, 275, 280, 331, 381
MaeIII GTNAC 4 cut(s) 276, 392, 725, 896
MbiI CCGCTC 1 cut(s) 149
MboII GAAGA 8 cut(s) 55, 126, 132, 150, 329, 524, 569, 1022
MflI RGATCY 6 cut(s) 336, 354, 420, 452, 630, 649
MhlI GDGCHC 1 cut(s) 469
MluCI AATT 3 cut(s) 488, 606, 658
MlyI GAGTC 1 cut(s) 697
MmeI TCCRAC 1 cut(s) 296
MseI TTAA 3 cut(s) 359, 702, 978
MslI CAYNNNNRTG 1 cut(s) 414
MspCI CTTAAG 1 cut(s) 358
MspI CCGG 4 cut(s) 152, 240, 872, 987
MspR9I CCNGG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 2 cut(s) 93, 167
MwoI GCNNNNNNNGC 1 cut(s) 961
NlaIII CATG 1 cut(s) 722
NlaIV GGNNCC 2 cut(s) 238, 651
NmuCI GTSAC 3 cut(s) 276, 392, 896
NspV TTCGAA 1 cut(s) 572
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 220, 312, 321
PfeI GAWTC 5 cut(s) 10, 225, 511, 574, 715
PinAI ACCGGT 1 cut(s) 986
PkrI GCNGC 4 cut(s) 190, 963, 1017, 1020
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
PshBI ATTAAT 1 cut(s) 702
PsiI TTATAA 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 469
Psp1406I AACGTT 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 2 cut(s) 238, 651
PspPI GGNCC 1 cut(s) 992
PsuI RGATCY 6 cut(s) 336, 354, 420, 452, 630, 649
RsaI GTAC 2 cut(s) 111, 673
RsaNI GTAC 2 cut(s) 110, 672
RseI CAYNNNNRTG 1 cut(s) 414
SacI GAGCTC 1 cut(s) 469
SaqAI TTAA 3 cut(s) 359, 702, 978
SatI GCNGC 4 cut(s) 189, 962, 1016, 1019
Sau96I GGNCC 1 cut(s) 992
SchI GAGTC 1 cut(s) 697
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 1 cut(s) 469
SfaNI GCATC 1 cut(s) 973
SfcI CTRYAG 1 cut(s) 340
SfuI TTCGAA 1 cut(s) 572
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 2 cut(s) 358, 434
SmoI CTYRAG 2 cut(s) 358, 434
Sse9I AATT 3 cut(s) 488, 606, 658
SsiI CCGC 8 cut(s) 91, 147, 165, 186, 189, 234, 1016, 1019
SspMI CTAG 2 cut(s) 209, 828
SstI GAGCTC 1 cut(s) 469
StyD4I CCNGG 1 cut(s) 423
TaaI ACNGT 4 cut(s) 50, 250, 325, 416
TaiI ACGT 6 cut(s) 64, 99, 278, 283, 334, 384
TaqI TCGA 2 cut(s) 351, 572
TasI AATT 3 cut(s) 488, 606, 658
TauI GCSGC 3 cut(s) 191, 1018, 1021
TfiI GAWTC 5 cut(s) 10, 225, 511, 574, 715
Tru1I TTAA 3 cut(s) 359, 702, 978
Tru9I TTAA 3 cut(s) 359, 702, 978
TseFI GTSAC 3 cut(s) 276, 392, 896
TseI GCWGC 1 cut(s) 961
Tsp45I GTSAC 3 cut(s) 276, 392, 896
TspDTI ATGAA 7 cut(s) 17, 56, 132, 524, 542, 651, 961
TspGWI ACGGA 2 cut(s) 321, 992
Vha464I CTTAAG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 992
VspI ATTAAT 1 cut(s) 702
XmiI GTMKAC 1 cut(s) 811
XspI CTAG 2 cut(s) 209, 828
ZraI GACGTC 3 cut(s) 97, 276, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.