Rmu_sc0002179.1_g000004

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002179.1
OG0001741
Physical Location & Seq
Forward (+)
28898 .. 30098
1201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002179.1_g000004.1.cds

Sequence Viewer

Length: 1077 bp
atggaccaaagttccgaaccccagcctcttcctccgagtcccagctcgtctccgccgggaccagagtcactgccgatcaacttctccgagttagagcgtgacaaaaaaccgattagaagaggagccggaggcacggtgtacaaagccgttcacaaacccaccggcctgctctacgcggtgaaagtgatcgacggcatcgacttccgtgactactcggtccggcgtcagatcatccaagagttcgaagtcctccgcgacgttgctaaccctaacatcgtcaagtgctacgacatgttcaaccacaacgacgatatttacgtcctcctagaatacatggagggaggttcattgaaaggcaagtacatcaacaacgagaaagctctctccgacttggctaggcagatcctcaccggcttgacttttctccacggacggcaggtagcccacatggatatcaagccctcgaatctcttgatcaactccaggcatgaagtcaagatcgcagatttcgttgggagagttttggccaagactatggatccgtgtaactcttctgtggggaacatagcatatatgtgcccggagagaatcaataccgatctaaatcacggaaattttgacgcctgcaccggcgatatatggagcgtcggagtcagcatcttggagttttacatagggcgagagagccgaaaaagagacgaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaagaaggggaagtaggccaagtagcacactggttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

358

Amino Acids

42.1

Weight (kDa)

4.05

Isoelectric Point (pI)

140.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 427
AccII CGCG 2 cut(s) 176, 255
AciI CCGC 3 cut(s) 53, 176, 253
AclWI GGATC 3 cut(s) 397, 533, 546
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 613
AcyI GRCGYC 2 cut(s) 223, 621
AfaI GTAC 2 cut(s) 140, 362
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 2 cut(s) 298, 352
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 2 cut(s) 45, 380
AluI AGCT 2 cut(s) 45, 380
Alw26I GTCTC 2 cut(s) 54, 690
AlwI GGATC 3 cut(s) 397, 533, 546
AoxI GGCC 3 cut(s) 163, 525, 1054
ApoI RAATTY 1 cut(s) 613
AspS9I GGNCC 3 cut(s) 4, 59, 217
AsuC2I CCSGG 2 cut(s) 57, 581
AsuHPI GGTGA 2 cut(s) 190, 400
AsuII TTCGAA 1 cut(s) 243
AvaII GGWCC 3 cut(s) 4, 59, 217
BaeGI GKGCMC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 527
BamHI GGATCC 1 cut(s) 538
BceAI ACGGC 3 cut(s) 131, 208, 449
BciT130I CCWGG 1 cut(s) 484
BclI TGATCA 1 cut(s) 474
BcnI CCSGG 2 cut(s) 57, 581
BcoDI GTCTC 2 cut(s) 54, 690
BfaI CTAG 2 cut(s) 326, 396
BfuAI ACCTGC 1 cut(s) 427
Bme1390I CCNGG 3 cut(s) 57, 484, 581
Bme18I GGWCC 3 cut(s) 4, 59, 217
BmgT120I GGNCC 3 cut(s) 4, 59, 217
BmiI GGNNCC 3 cut(s) 60, 124, 540
BmrFI CCNGG 3 cut(s) 57, 484, 581
BmsI GCATC 2 cut(s) 204, 666
BoxI GACNNNNGTC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 466
Bpu14I TTCGAA 1 cut(s) 243
BpuMI CCSGG 2 cut(s) 57, 581
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 2 cut(s) 223, 621
BsaJI CCNNGG 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bse118I RCCGGY 3 cut(s) 161, 410, 629
Bse1I ACTGG 1 cut(s) 1073
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 1 cut(s) 231
BseJI GATNNNNATC 1 cut(s) 603
BseNI ACTGG 1 cut(s) 1073
BseRI GAGGAG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 581
BseYI CCCAGC 2 cut(s) 21, 41
BsgI GTGCAG 1 cut(s) 610
Bsh1236I CGCG 2 cut(s) 176, 255
BshFI GGCC 3 cut(s) 165, 527, 1056
BsiSI CCGG 7 cut(s) 56, 126, 162, 220, 411, 581, 630
BslFI GGGAC 2 cut(s) 24, 72
BsmAI GTCTC 2 cut(s) 54, 690
BsmBI CGTCTC 2 cut(s) 54, 690
BsmFI GGGAC 2 cut(s) 24, 72
BsnI GGCC 3 cut(s) 165, 527, 1056
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 581
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
BspACI CCGC 3 cut(s) 53, 176, 253
BspANI GGCC 3 cut(s) 165, 527, 1056
BspFNI CGCG 2 cut(s) 176, 255
BspLI GGNNCC 3 cut(s) 60, 124, 540
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 3 cut(s) 397, 533, 546
BspT104I TTCGAA 1 cut(s) 243
BsrFI RCCGGY 3 cut(s) 161, 410, 629
BsrGI TGTACA 1 cut(s) 138
BsrI ACTGG 1 cut(s) 1073
BssAI RCCGGY 3 cut(s) 161, 410, 629
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
BssNI GRCGYC 2 cut(s) 223, 621
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 3 cut(s) 33, 112, 556
BstACI GRCGYC 2 cut(s) 223, 621
BstAUI TGTACA 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 167, 625
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 1 cut(s) 231
BstFNI CGCG 2 cut(s) 176, 255
BstKTI GATC 8 cut(s) 78, 189, 231, 405, 477, 501, 541, 601
BstMAI GTCTC 2 cut(s) 54, 690
BstMBI GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 1 cut(s) 295
BstPAI GACNNNNGTC 1 cut(s) 64
BstSCI CCNGG 3 cut(s) 55, 482, 579
BstSLI GKGCMC 1 cut(s) 581
BstUI CGCG 2 cut(s) 176, 255
BstX2I RGATCY 2 cut(s) 402, 538
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 2 cut(s) 402, 538
BsuRI GGCC 3 cut(s) 165, 527, 1056
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 1 cut(s) 231
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 68, 1066
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 2 cut(s) 167, 625
Cfr10I RCCGGY 3 cut(s) 161, 410, 629
Cfr13I GGNCC 3 cut(s) 4, 59, 217
CpoI CGGWCCG 1 cut(s) 217
CseI GACGC 3 cut(s) 212, 629, 634
Csp6I GTAC 2 cut(s) 139, 361
CspI CGGWCCG 1 cut(s) 217
CviAII CATG 4 cut(s) 292, 334, 448, 488
CviQI GTAC 2 cut(s) 139, 361
DpnI GATC 8 cut(s) 77, 188, 230, 404, 476, 500, 540, 600
DpnII GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 3 cut(s) 33, 112, 556
EarI CTCTTC 3 cut(s) 33, 112, 556
EciI GGCGGA 1 cut(s) 42
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 3 cut(s) 4, 59, 217
EcoRII CCWGG 1 cut(s) 482
EcoRV GATATC 1 cut(s) 454
Esp3I CGTCTC 2 cut(s) 54, 690
FaeI CATG 4 cut(s) 295, 337, 451, 491
FaqI GGGAC 2 cut(s) 24, 72
FatI CATG 4 cut(s) 291, 333, 447, 487
FbaI TGATCA 1 cut(s) 474
FokI GGATG 1 cut(s) 218
FspBI CTAG 2 cut(s) 326, 396
GsaI CCCAGC 2 cut(s) 25, 45
GsuI CTGGAG 1 cut(s) 466
HaeIII GGCC 3 cut(s) 165, 527, 1056
HapII CCGG 7 cut(s) 56, 126, 162, 220, 411, 581, 630
HgaI GACGC 3 cut(s) 212, 629, 634
Hin1I GRCGYC 2 cut(s) 223, 621
Hin1II CATG 4 cut(s) 295, 337, 451, 491
HinfI GANTC 5 cut(s) 37, 65, 466, 588, 651
HpaII CCGG 7 cut(s) 56, 126, 162, 220, 411, 581, 630
HphI GGTGA 2 cut(s) 190, 400
Hpy166II GTNNAC 2 cut(s) 139, 151
Hpy188I TCNGA 6 cut(s) 16, 36, 88, 228, 388, 650
Hpy188III TCNNGA 2 cut(s) 472, 496
Hpy8I GTNNAC 2 cut(s) 139, 151
Hpy99I CGWCG 4 cut(s) 194, 260, 311, 650
HpyAV CCTTC 1 cut(s) 1037
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 258, 318
HpyCH4V TGCA 1 cut(s) 627
HpySE526I ACGT 2 cut(s) 258, 318
Hsp92I GRCGYC 2 cut(s) 223, 621
Hsp92II CATG 4 cut(s) 295, 337, 451, 491
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
LmnI GCTCC 2 cut(s) 122, 642
LweI GCATC 2 cut(s) 204, 666
MaeI CTAG 2 cut(s) 326, 396
MaeII ACGT 2 cut(s) 258, 318
MaeIII GTNAC 4 cut(s) 66, 98, 206, 545
MalI GATC 8 cut(s) 77, 188, 230, 404, 476, 500, 540, 600
MboI GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
MflI RGATCY 2 cut(s) 402, 538
MhlI GDGCHC 1 cut(s) 581
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 1 cut(s) 613
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 3 cut(s) 46, 74, 660
MmeI TCCRAC 2 cut(s) 411, 628
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MslI CAYNNNNRTG 1 cut(s) 574
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 7 cut(s) 56, 126, 162, 220, 411, 581, 630
MspR9I CCNGG 3 cut(s) 57, 484, 581
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 2 cut(s) 176, 255
NciI CCSGG 2 cut(s) 57, 581
NdeII GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
NlaIII CATG 4 cut(s) 295, 337, 451, 491
NlaIV GGNNCC 3 cut(s) 60, 124, 540
NmuCI GTSAC 3 cut(s) 66, 98, 206
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 243
PciI ACATGT 1 cut(s) 291
PcsI WCGNNNNNNNCGW 3 cut(s) 195, 315, 507
PfeI GAWTC 2 cut(s) 466, 588
PleI GAGTC 3 cut(s) 45, 73, 659
PpsI GAGTC 3 cut(s) 45, 73, 659
PscI ACATGT 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 482
PspFI CCCAGC 2 cut(s) 21, 41
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 3 cut(s) 60, 124, 540
PspPI GGNCC 3 cut(s) 4, 59, 217
PsuI RGATCY 2 cut(s) 402, 538
RsaI GTAC 2 cut(s) 140, 362
RsaNI GTAC 2 cut(s) 139, 361
RseI CAYNNNNRTG 1 cut(s) 574
Rsr2I CGGWCCG 1 cut(s) 217
RsrII CGGWCCG 1 cut(s) 217
Sau3AI GATC 8 cut(s) 75, 186, 228, 402, 474, 498, 538, 598
Sau96I GGNCC 3 cut(s) 4, 59, 217
SchI GAGTC 3 cut(s) 46, 74, 660
ScrFI CCNGG 3 cut(s) 57, 484, 581
SduI GDGCHC 1 cut(s) 581
SetI ASST 6 cut(s) 47, 261, 321, 346, 382, 441
SfaNI GCATC 2 cut(s) 204, 666
SfuI TTCGAA 1 cut(s) 243
SgrAI CRCCGGYG 1 cut(s) 629
SinI GGWCC 3 cut(s) 4, 59, 217
SmiMI CAYNNNNRTG 1 cut(s) 574
Sse9I AATT 1 cut(s) 613
SsiI CCGC 3 cut(s) 53, 176, 253
SspMI CTAG 2 cut(s) 326, 396
StyD4I CCNGG 3 cut(s) 55, 482, 579
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 261, 321
TaqI TCGA 4 cut(s) 189, 198, 243, 464
TaqII GACCGA 1 cut(s) 205
TasI AATT 1 cut(s) 613
TatI WGTACW 2 cut(s) 138, 360
TfiI GAWTC 2 cut(s) 466, 588
TscAI CASTG 2 cut(s) 75, 1073
TseFI GTSAC 3 cut(s) 66, 98, 206
Tsp45I GTSAC 3 cut(s) 66, 98, 206
TspDTI ATGAA 2 cut(s) 336, 504
TspGWI ACGGA 4 cut(s) 194, 444, 531, 624
TspRI CASTG 2 cut(s) 75, 1073
VpaK11BI GGWCC 3 cut(s) 4, 59, 217
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 295
XspI CTAG 2 cut(s) 326, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.