Rw2G039150

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001741
Physical Location & Seq
Forward (+)
64115202 .. 64117442
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039150.1

Sequence Viewer

Length: 1065 bp
ATGAGACCGAATAATATGAGTCAGATGCCGCCGCCGCCGCCGCCGGGAGTTGTGGCCGCCAGAAAGGACCCGTCGGGGAAGACACGGCGTCGTCCTGACCTGAGTCTACCGATGCCGAGGCGGGACACGTCTCTGGCCGTGCCGTTGCCTCTGCCGCCGGGACCGAGCTCGGCTCCGTCCGGGTCCGAGGCCGTGATGATCAACTTCTCCGAGCTGGAGCGGGCGAACCGGATTGGGAGCGGAGCGGGCGGCACGGTCTACAAGGTTATTCACAAGCCGACGGGGCGGCTCTACGCGCTGAAAGTGATCTACGGCAACCACGACGAGTCGACCCGGCACCAGATCTGCCGCGAGATCCAAATCCTCCGCGACGTCGATAACCCTAACGTCGTCAAGTGCCACGATATGTTTGACCACAACGGCGAGATCCAGGTGCTGCTGGAGTTCATGGACGGCGGTTCGTTAGAGGGGAAGCACATCGCCAACGAGAAAAGCCTCTCCGACTTGGCACGGCAGATCCTCACCGGATTGGCCTACCTCCACAAACGGCACATCGTCCACCGCGACATCAAGCCTTCGAATCTCTTGATCAACGCCCGGAATCAGGTCAAGATCGCCGATTTCGGAGTCAGCCGGATTTTGGCGCAGACTATGGACCCGTGTAACTCCTCTGTAGGGACCATAGCCTACATGAGCCCGGAGAGGATCAACACCGATCTCAATCAGGGCAGGTACGACGGCTACGCCGGCGATATCTGGAGCCTGGGGGTCAGCATCTTGGAGTTTTACATGGGCCGATTTCCGTTCAATGTGCAGCGGGCTCAGGGAGATTGGGCCAGCTTAATGTGGGCCATTTGTATGTCTCAGCCGCCTGAGGCTCCTCCGACGGCCTCCAGAGATTTCCGGCATTTCATTGCTTGCTGTTTGCAGAGAGAGCCCAACAGGAGAATGTCGGCGCAGCAGTTGTTGCAGCACCCATTTATCTCCGGCAATGGGGGCGGGCCGCCGCAGGTTCATCAGAATCTGCACACTCTTCTACCACCGCCACGCCCGCTCCCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

354

Amino Acids

39.26

Weight (kDa)

9.28

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 74 - 328 1.2e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 74 - 324 8.9e-39 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 158 - 273 6.1e-07 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
Acc36I ACCTGC 2 cut(s) 720, 1000
AccB1I GGYRCC 1 cut(s) 336
AccBSI CCGCTC 4 cut(s) 220, 240, 245, 1054
AccI GTMKAC 3 cut(s) 106, 258, 329
AccII CGCG 4 cut(s) 296, 351, 369, 564
AclWI GGATC 4 cut(s) 349, 421, 511, 713
AcoI YGGCCR 2 cut(s) 54, 135
AcyI GRCGYC 2 cut(s) 88, 372
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 5 cut(s) 44, 604, 640, 675, 993
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 1 cut(s) 808
AhdI GACNNNNNGTC 1 cut(s) 87
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 2 cut(s) 429, 762
AluBI AGCT 3 cut(s) 168, 214, 840
AluI AGCT 3 cut(s) 168, 214, 840
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 135, 867
AlwI GGATC 4 cut(s) 349, 421, 511, 713
AlwNI CAGNNNCTG 2 cut(s) 436, 1024
AoxI GGCC 9 cut(s) 54, 135, 189, 531, 793, 834, 849, 888, 1001
ApeKI GCWGC 4 cut(s) 436, 814, 958, 970
AspLEI GCGC 3 cut(s) 298, 646, 958
AspS9I GGNCC 9 cut(s) 67, 161, 183, 655, 678, 793, 834, 849, 1001
AsuC2I CCSGG 6 cut(s) 45, 159, 181, 334, 598, 698
AsuHPI GGTGA 1 cut(s) 514
AsuII TTCGAA 1 cut(s) 578
AvaII GGWCC 5 cut(s) 67, 161, 183, 655, 678
AxyI CCTNAGG 1 cut(s) 873
BanI GGYRCC 1 cut(s) 336
BanII GRGCYC 4 cut(s) 170, 698, 823, 939
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 4 cut(s) 423, 826, 970, 982
BciT130I CCWGG 2 cut(s) 431, 764
BclI TGATCA 2 cut(s) 198, 588
BcnI CCSGG 6 cut(s) 45, 159, 181, 334, 598, 698
BcoDI GTCTC 2 cut(s) 135, 867
BfmI CTRYAG 1 cut(s) 672
BfuAI ACCTGC 2 cut(s) 720, 1000
BglI GCCNNNNNGGC 1 cut(s) 283
BglII AGATCT 1 cut(s) 342
Bme1390I CCNGG 8 cut(s) 45, 159, 181, 334, 431, 598, 698, 764
Bme18I GGWCC 5 cut(s) 67, 161, 183, 655, 678
BmeRI GACNNNNNGTC 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 9 cut(s) 67, 161, 183, 655, 678, 793, 834, 849, 1001
BmiI GGNNCC 9 cut(s) 69, 162, 174, 184, 338, 657, 679, 761, 879
BmrFI CCNGG 8 cut(s) 45, 159, 181, 334, 431, 598, 698, 764
BmsI GCATC 3 cut(s) 15, 102, 783
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 86
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 4 cut(s) 236, 461, 778, 877
Bpu10I CCTNAGC 1 cut(s) 822
Bpu14I TTCGAA 1 cut(s) 578
BpuMI CCSGG 6 cut(s) 45, 159, 181, 334, 598, 698
BsaBI GATNNNNATC 2 cut(s) 359, 720
BsaHI GRCGYC 2 cut(s) 88, 372
BsaJI CCNNGG 3 cut(s) 116, 186, 763
BsaWI WCCGGW 2 cut(s) 228, 524
BsaXI ACNNNNNCTCC 1 cut(s) 1038
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 604, 640, 675, 993
Bse118I RCCGGY 1 cut(s) 746
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 2 cut(s) 912, 997
Bse8I GATNNNNATC 2 cut(s) 359, 720
BseBI CCWGG 2 cut(s) 431, 764
BseDI CCNNGG 3 cut(s) 116, 186, 763
BseJI GATNNNNATC 2 cut(s) 359, 720
BseLI CCNNNNNNNGG 5 cut(s) 44, 604, 640, 675, 993
BseMI GCAATG 2 cut(s) 912, 997
BseMII CTCAG 4 cut(s) 92, 836, 864, 878
BseRI GAGGAG 2 cut(s) 658, 870
BseXI GCAGC 4 cut(s) 423, 826, 970, 982
BsgI GTGCAG 2 cut(s) 833, 1010
Bsh1236I CGCG 4 cut(s) 296, 351, 369, 564
BshFI GGCC 9 cut(s) 56, 137, 191, 533, 795, 836, 851, 890, 1003
BshNI GGYRCC 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 170
BslFI GGGAC 3 cut(s) 137, 174, 691
BslI CCNNNNNNNGG 5 cut(s) 44, 604, 640, 675, 993
BsmAI GTCTC 2 cut(s) 135, 867
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 3 cut(s) 137, 174, 691
BsnI GGCC 9 cut(s) 56, 137, 191, 533, 795, 836, 851, 890, 1003
Bsp119I TTCGAA 1 cut(s) 578
Bsp1286I GDGCHC 4 cut(s) 170, 698, 823, 939
BspANI GGCC 9 cut(s) 56, 137, 191, 533, 795, 836, 851, 890, 1003
BspCNI CTCAG 4 cut(s) 93, 835, 865, 877
BspFNI CGCG 4 cut(s) 296, 351, 369, 564
BspLI GGNNCC 9 cut(s) 69, 162, 174, 184, 338, 657, 679, 761, 879
BspMI ACCTGC 2 cut(s) 720, 1000
BspPI GGATC 4 cut(s) 349, 421, 511, 713
BspT104I TTCGAA 1 cut(s) 578
BspT107I GGYRCC 1 cut(s) 336
BsrBI CCGCTC 4 cut(s) 220, 240, 245, 1054
BsrDI GCAATG 2 cut(s) 912, 997
BsrFI RCCGGY 1 cut(s) 746
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 116, 186, 763
BssNI GRCGYC 2 cut(s) 88, 372
Bst2UI CCWGG 2 cut(s) 431, 764
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 1038
BstACI GRCGYC 2 cut(s) 88, 372
BstAPI GCANNNNNTGC 1 cut(s) 967
BstBI TTCGAA 1 cut(s) 578
BstC8I GCNNGC 8 cut(s) 222, 247, 748, 819, 838, 919, 1001, 1052
BstDEI CTNAG 5 cut(s) 101, 822, 864, 873, 1062
BstFNI CGCG 4 cut(s) 296, 351, 369, 564
BstHHI GCGC 3 cut(s) 298, 646, 958
BstMAI GTCTC 2 cut(s) 135, 867
BstNI CCWGG 2 cut(s) 431, 764
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 8 cut(s) 43, 157, 179, 332, 429, 596, 696, 762
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 4 cut(s) 296, 351, 369, 564
BstV1I GCAGC 4 cut(s) 423, 826, 970, 982
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 4 cut(s) 342, 354, 426, 516
BstYI RGATCY 4 cut(s) 342, 354, 426, 516
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 9 cut(s) 56, 137, 191, 533, 795, 836, 851, 890, 1003
BtgZI GCGATG 1 cut(s) 463
BtrI CACGTC 1 cut(s) 129
BveI ACCTGC 2 cut(s) 720, 1000
Cac8I GCNNGC 8 cut(s) 222, 247, 748, 819, 838, 919, 1001, 1052
CaiI CAGNNNCTG 2 cut(s) 436, 1024
CfoI GCGC 3 cut(s) 298, 646, 958
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 9 cut(s) 67, 161, 183, 655, 678, 793, 834, 849, 1001
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 733
CviAII CATG 3 cut(s) 448, 691, 790
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 5 cut(s) 101, 822, 864, 873, 1062
DriI GACNNNNNGTC 1 cut(s) 87
EaeI YGGCCR 2 cut(s) 54, 135
Eam1104I CTCTTC 1 cut(s) 1038
Eam1105I GACNNNNNGTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 1038
Ecl136II GAGCTC 1 cut(s) 168
Eco24I GRGCYC 4 cut(s) 170, 698, 823, 939
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 5 cut(s) 67, 161, 183, 655, 678
Eco53kI GAGCTC 1 cut(s) 168
Eco81I CCTNAGG 1 cut(s) 873
EcoICRI GAGCTC 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 429, 762
EcoRV GATATC 1 cut(s) 754
EcoT38I GRGCYC 4 cut(s) 170, 698, 823, 939
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 3 cut(s) 451, 694, 793
FaiI YATR 8 cut(s) 17, 407, 449, 653, 683, 692, 791, 860
FaqI GGGAC 3 cut(s) 137, 174, 691
FatI CATG 3 cut(s) 447, 690, 789
FauI CCCGC 6 cut(s) 114, 213, 238, 810, 992, 1059
FbaI TGATCA 2 cut(s) 198, 588
FblI GTMKAC 3 cut(s) 106, 258, 329
FriOI GRGCYC 4 cut(s) 170, 698, 823, 939
GlaI GCGC 3 cut(s) 297, 645, 957
GsuI CTGGAG 4 cut(s) 236, 461, 778, 877
HaeIII GGCC 9 cut(s) 56, 137, 191, 533, 795, 836, 851, 890, 1003
HgaI GACGC 1 cut(s) 77
HhaI GCGC 3 cut(s) 298, 646, 958
Hin1I GRCGYC 2 cut(s) 88, 372
Hin1II CATG 3 cut(s) 451, 694, 793
Hin6I GCGC 3 cut(s) 296, 644, 956
HinP1I GCGC 3 cut(s) 296, 644, 956
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 7 cut(s) 19, 103, 326, 580, 601, 627, 1021
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 4 cut(s) 107, 259, 330, 559
Hpy188I TCNGA 7 cut(s) 24, 187, 211, 502, 626, 885, 1020
Hpy188III TCNNGA 5 cut(s) 95, 586, 610, 757, 894
Hpy8I GTNNAC 4 cut(s) 107, 259, 330, 559
Hpy99I CGWCG 9 cut(s) 76, 93, 283, 326, 374, 377, 392, 740, 889
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 3 cut(s) 128, 372, 387
HpyCH4V TGCA 4 cut(s) 814, 928, 970, 1027
HpyF3I CTNAG 5 cut(s) 101, 822, 864, 873, 1062
HpySE526I ACGT 3 cut(s) 128, 372, 387
Hsp92I GRCGYC 2 cut(s) 88, 372
Hsp92II CATG 3 cut(s) 451, 694, 793
HspAI GCGC 3 cut(s) 296, 644, 956
KroI GCCGGC 1 cut(s) 746
KroNI GCCGGC 1 cut(s) 748
Ksp22I TGATCA 2 cut(s) 198, 588
LmnI GCTCC 7 cut(s) 178, 217, 237, 242, 759, 883, 1059
Lsp1109I GCAGC 4 cut(s) 423, 826, 970, 982
LweI GCATC 3 cut(s) 15, 102, 783
MaeII ACGT 3 cut(s) 128, 372, 387
MaeIII GTNAC 1 cut(s) 662
MbiI CCGCTC 4 cut(s) 220, 240, 245, 1054
MboII GAAGA 2 cut(s) 91, 1025
MflI RGATCY 4 cut(s) 342, 354, 426, 516
MhlI GDGCHC 4 cut(s) 170, 698, 823, 939
MlyI GAGTC 4 cut(s) 28, 112, 335, 636
MmeI TCCRAC 2 cut(s) 525, 908
MreI CGCCGGCG 1 cut(s) 746
MroNI GCCGGC 1 cut(s) 746
MseI TTAA 1 cut(s) 842
MslI CAYNNNNRTG 1 cut(s) 857
MspA1I CMGCKG 1 cut(s) 817
MspR9I CCNGG 8 cut(s) 45, 159, 181, 334, 431, 598, 698, 764
MvaI CCWGG 2 cut(s) 431, 764
MvnI CGCG 4 cut(s) 296, 351, 369, 564
NaeI GCCGGC 1 cut(s) 748
NciI CCSGG 6 cut(s) 45, 159, 181, 334, 598, 698
NgoMIV GCCGGC 1 cut(s) 746
NlaIII CATG 3 cut(s) 451, 694, 793
NlaIV GGNNCC 9 cut(s) 69, 162, 174, 184, 338, 657, 679, 761, 879
NmeAIII GCCGAG 2 cut(s) 141, 149
NspV TTCGAA 1 cut(s) 578
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 561
PdiI GCCGGC 1 cut(s) 748
PfeI GAWTC 3 cut(s) 580, 601, 1021
PleI GAGTC 4 cut(s) 27, 111, 334, 635
PpsI GAGTC 4 cut(s) 27, 111, 334, 635
PpuMI RGGWCCY 1 cut(s) 67
PshAI GACNNNNGTC 1 cut(s) 102
Psp124BI GAGCTC 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 429, 762
PspGI CCWGG 2 cut(s) 429, 762
PspN4I GGNNCC 9 cut(s) 69, 162, 174, 184, 338, 657, 679, 761, 879
PspPI GGNCC 9 cut(s) 67, 161, 183, 655, 678, 793, 834, 849, 1001
PspPPI RGGWCCY 1 cut(s) 67
PstNI CAGNNNCTG 2 cut(s) 436, 1024
PsuI RGATCY 4 cut(s) 342, 354, 426, 516
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 857
SacI GAGCTC 1 cut(s) 170
SalI GTCGAC 1 cut(s) 328
SaqAI TTAA 1 cut(s) 842
Sau96I GGNCC 9 cut(s) 67, 161, 183, 655, 678, 793, 834, 849, 1001
SchI GAGTC 4 cut(s) 28, 112, 335, 636
ScrFI CCNGG 8 cut(s) 45, 159, 181, 334, 431, 598, 698, 764
SduI GDGCHC 4 cut(s) 170, 698, 823, 939
SfaNI GCATC 3 cut(s) 15, 102, 783
SfcI CTRYAG 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 578
SgrAI CRCCGGYG 1 cut(s) 746
SinI GGWCC 5 cut(s) 67, 161, 183, 655, 678
SmiMI CAYNNNNRTG 1 cut(s) 857
SstI GAGCTC 1 cut(s) 170
StyD4I CCNGG 8 cut(s) 43, 157, 179, 332, 429, 596, 696, 762
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 3 cut(s) 131, 375, 390
TaqI TCGA 3 cut(s) 329, 375, 578
TaqII GACCGA 2 cut(s) 22, 178
TfiI GAWTC 3 cut(s) 580, 601, 1021
Tru1I TTAA 1 cut(s) 842
Tru9I TTAA 1 cut(s) 842
TseI GCWGC 4 cut(s) 436, 814, 958, 970
TspDTI ATGAA 3 cut(s) 436, 901, 1004
TspGWI ACGGA 2 cut(s) 165, 792
VpaK11BI GGWCC 5 cut(s) 67, 161, 183, 655, 678
XmiI GTMKAC 3 cut(s) 106, 258, 329
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.