RLG00000020488

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001741
Physical Location & Seq
Forward (+)
65542156 .. 65543172
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020488

Sequence Viewer

Length: 1017 bp
ATGCCGCCGCCGCCGCCGGGAGGTGTGGCCGCCAGACGGCGTCGTCCTGACCTGAGTCTACCGATGCCGAGGCGGGACACGTCTCTGGCCGTGCCGTTGCCTCTGCCGCCGGGACCGAGCTCGGCTCCGTCCGGGTCCGAGGCCGTGATGATCAACTTCTCCGAGCTGGAGCGGGCGAACCGGATTGGGAGCGGAGCCGGCGGCACGGTCTACAAGGTTATTCACAAGCCGACCGGGCGGCTCTACGCGCTGAAGGTCATCTACGGCAACCACGACGAGTCGACCCGGCACCAGATCTGCCGCGAGATCCAAATCCTCCGCGACGTCGATAACCCTAACGTCGTCAAGTGCCACGATATGTTTGACCACAACGGCGAGATCCAGGTGCTGCTGGAGTTCATGGACGGCGGTTCGTTAGAGGGGAAGCACATAGCCAACGAGAAAAGCCTCTCCGACTTGGCACGGCAGATCCTCACCGGATTGGCCTACCTCCACAAACGGCACATCGTCCACCGCGATATCAAGCCTTCGAATCTCTTGATCAACGCCCGGAATCAGGTCAAGATCGCCGATTTCGGAGTCAGCCGGATTTTGGCGCAGACTATGGACCCGTGTAACTCCTCTGTAGGGACCATAGCCTACATGAGCCCGGAGAGGATCAACACCGATCTCAATCAGGGCAGGTACGACGGCTACGCCGGCGATATTTGGAGCCTGGGGGTCAGCATCTTGGAGTTTTACATGGGCCGATTTCCGTTCAATGTGCAGCGGGCTCAGGGAGATTGGGCCAGCTTAATGTGGGCCATTTGTATGTCTCAGCCGCCTGAGGCTCCGCCGACGGCCTCCAGAGAGTTCCGGCATTTCATTGCTTGCTGTTTGCAGAGAGAGCCGAACAGGAGAATGTCGGCGCAGCAGTTGTTGCAGCACCCATTTATCTCCGGCAATGGGGGCGGGCCGCCGCAGGTTCATCAGAATCTGCACACTCTTCTACCACCGCCACGCCCGCTTCCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

339

Amino Acids

37.46

Weight (kDa)

9.06

Isoelectric Point (pI)

63.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 58 - 312 5.8e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 58 - 308 3.8e-39 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 142 - 257 5.5e-07 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 42
AatII GACGTC 1 cut(s) 327
Acc36I ACCTGC 2 cut(s) 672, 952
AccB1I GGYRCC 1 cut(s) 288
AccBSI CCGCTC 2 cut(s) 172, 192
AccI GTMKAC 3 cut(s) 58, 210, 281
AccII CGCG 4 cut(s) 248, 303, 321, 516
AclWI GGATC 4 cut(s) 301, 373, 463, 665
AcoI YGGCCR 2 cut(s) 27, 87
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 2 cut(s) 40, 324
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 7 cut(s) 17, 20, 36, 556, 592, 627, 945
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 1 cut(s) 760
AjiI CACGTC 1 cut(s) 81
AjnI CCWGG 2 cut(s) 381, 714
AluBI AGCT 3 cut(s) 120, 166, 792
AluI AGCT 3 cut(s) 120, 166, 792
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 2 cut(s) 87, 819
AlwI GGATC 4 cut(s) 301, 373, 463, 665
AlwNI CAGNNNCTG 2 cut(s) 388, 976
AoxI GGCC 9 cut(s) 27, 87, 141, 483, 745, 786, 801, 840, 953
ApeKI GCWGC 4 cut(s) 388, 766, 910, 922
AspLEI GCGC 3 cut(s) 250, 598, 910
AspS9I GGNCC 8 cut(s) 113, 135, 607, 630, 745, 786, 801, 953
AsuC2I CCSGG 7 cut(s) 18, 111, 133, 235, 286, 550, 650
AsuHPI GGTGA 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 530
AvaII GGWCC 4 cut(s) 113, 135, 607, 630
AxyI CCTNAGG 1 cut(s) 825
BanI GGYRCC 1 cut(s) 288
BanII GRGCYC 3 cut(s) 122, 650, 775
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 4 cut(s) 375, 778, 922, 934
BciT130I CCWGG 2 cut(s) 383, 716
BclI TGATCA 2 cut(s) 150, 540
BcnI CCSGG 7 cut(s) 18, 111, 133, 235, 286, 550, 650
BcoDI GTCTC 2 cut(s) 87, 819
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 2 cut(s) 672, 952
BglI GCCNNNNNGGC 1 cut(s) 235
BglII AGATCT 1 cut(s) 294
Bme1390I CCNGG 9 cut(s) 18, 111, 133, 235, 286, 383, 550, 650, 716
Bme18I GGWCC 4 cut(s) 113, 135, 607, 630
BmgBI CACGTC 1 cut(s) 81
BmgT120I GGNCC 8 cut(s) 113, 135, 607, 630, 745, 786, 801, 953
BmiI GGNNCC 9 cut(s) 114, 126, 136, 196, 290, 609, 631, 713, 831
BmrFI CCNGG 9 cut(s) 18, 111, 133, 235, 286, 383, 550, 650, 716
BmsI GCATC 2 cut(s) 54, 735
BoxI GACNNNNGTC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 109, 141
BpmI CTGGAG 3 cut(s) 188, 413, 829
Bpu10I CCTNAGC 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 530
BpuMI CCSGG 7 cut(s) 18, 111, 133, 235, 286, 550, 650
BsaBI GATNNNNATC 2 cut(s) 311, 672
BsaHI GRCGYC 2 cut(s) 40, 324
BsaJI CCNNGG 3 cut(s) 68, 138, 715
BsaWI WCCGGW 2 cut(s) 180, 476
Bsc4I CCNNNNNNNGG 7 cut(s) 17, 20, 36, 556, 592, 627, 945
Bse118I RCCGGY 2 cut(s) 197, 698
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 2 cut(s) 864, 949
Bse8I GATNNNNATC 2 cut(s) 311, 672
BseBI CCWGG 2 cut(s) 383, 716
BseDI CCNNGG 3 cut(s) 68, 138, 715
BseJI GATNNNNATC 2 cut(s) 311, 672
BseLI CCNNNNNNNGG 7 cut(s) 17, 20, 36, 556, 592, 627, 945
BseMI GCAATG 2 cut(s) 864, 949
BseMII CTCAG 4 cut(s) 44, 788, 816, 830
BseRI GAGGAG 1 cut(s) 610
BseXI GCAGC 4 cut(s) 375, 778, 922, 934
BsgI GTGCAG 2 cut(s) 785, 962
Bsh1236I CGCG 4 cut(s) 248, 303, 321, 516
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 9 cut(s) 29, 89, 143, 485, 747, 788, 803, 842, 955
BshNI GGYRCC 1 cut(s) 288
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 122
BslFI GGGAC 3 cut(s) 89, 126, 643
BslI CCNNNNNNNGG 7 cut(s) 17, 20, 36, 556, 592, 627, 945
BsmAI GTCTC 2 cut(s) 87, 819
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 3 cut(s) 89, 126, 643
BsnI GGCC 9 cut(s) 29, 89, 143, 485, 747, 788, 803, 842, 955
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 3 cut(s) 122, 650, 775
Bsp143I GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
BspANI GGCC 9 cut(s) 29, 89, 143, 485, 747, 788, 803, 842, 955
BspCNI CTCAG 4 cut(s) 45, 787, 817, 829
BspFNI CGCG 4 cut(s) 248, 303, 321, 516
BspLI GGNNCC 9 cut(s) 114, 126, 136, 196, 290, 609, 631, 713, 831
BspMI ACCTGC 2 cut(s) 672, 952
BspPI GGATC 4 cut(s) 301, 373, 463, 665
BspT104I TTCGAA 1 cut(s) 530
BspT107I GGYRCC 1 cut(s) 288
BsrBI CCGCTC 2 cut(s) 172, 192
BsrDI GCAATG 2 cut(s) 864, 949
BsrFI RCCGGY 2 cut(s) 197, 698
BssAI RCCGGY 2 cut(s) 197, 698
BssECI CCNNGG 3 cut(s) 68, 138, 715
BssMI GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
BssNI GRCGYC 2 cut(s) 40, 324
Bst2UI CCWGG 2 cut(s) 383, 716
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 990
BstACI GRCGYC 2 cut(s) 40, 324
BstAPI GCANNNNNTGC 1 cut(s) 919
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 8 cut(s) 174, 199, 700, 771, 790, 871, 953, 1004
BstDEI CTNAG 5 cut(s) 53, 774, 816, 825, 1014
BstFNI CGCG 4 cut(s) 248, 303, 321, 516
BstHHI GCGC 3 cut(s) 250, 598, 910
BstKTI GATC 9 cut(s) 153, 297, 309, 381, 471, 543, 567, 660, 670
BstMAI GTCTC 2 cut(s) 87, 819
BstMBI GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
BstMCI CGRYCG 1 cut(s) 234
BstNI CCWGG 2 cut(s) 383, 716
BstPAI GACNNNNGTC 1 cut(s) 54
BstSCI CCNGG 9 cut(s) 16, 109, 131, 233, 284, 381, 548, 648, 714
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 4 cut(s) 248, 303, 321, 516
BstV1I GCAGC 4 cut(s) 375, 778, 922, 934
BstX2I RGATCY 4 cut(s) 294, 306, 378, 468
BstYI RGATCY 4 cut(s) 294, 306, 378, 468
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 9 cut(s) 29, 89, 143, 485, 747, 788, 803, 842, 955
BtrI CACGTC 1 cut(s) 81
BveI ACCTGC 2 cut(s) 672, 952
Cac8I GCNNGC 8 cut(s) 174, 199, 700, 771, 790, 871, 953, 1004
CaiI CAGNNNCTG 2 cut(s) 388, 976
CfoI GCGC 3 cut(s) 250, 598, 910
Cfr10I RCCGGY 2 cut(s) 197, 698
Cfr13I GGNCC 8 cut(s) 113, 135, 607, 630, 745, 786, 801, 953
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 685
CviAII CATG 3 cut(s) 400, 643, 742
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 5 cut(s) 53, 774, 816, 825, 1014
DpnI GATC 9 cut(s) 152, 296, 308, 380, 470, 542, 566, 659, 669
DpnII GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
DrdI GACNNNNNNGTC 1 cut(s) 42
DseDI GACNNNNNNGTC 1 cut(s) 42
EaeI YGGCCR 2 cut(s) 27, 87
Eam1104I CTCTTC 1 cut(s) 990
EarI CTCTTC 1 cut(s) 990
EciI GGCGGA 1 cut(s) 822
Ecl136II GAGCTC 1 cut(s) 120
Eco24I GRGCYC 3 cut(s) 122, 650, 775
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 4 cut(s) 113, 135, 607, 630
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 825
EcoICRI GAGCTC 1 cut(s) 120
EcoRII CCWGG 2 cut(s) 381, 714
EcoRV GATATC 1 cut(s) 520
EcoT38I GRGCYC 3 cut(s) 122, 650, 775
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 3 cut(s) 403, 646, 745
FaiI YATR 8 cut(s) 359, 401, 431, 605, 635, 644, 743, 812
FaqI GGGAC 3 cut(s) 89, 126, 643
FatI CATG 3 cut(s) 399, 642, 741
FauI CCCGC 5 cut(s) 66, 165, 762, 944, 1011
FbaI TGATCA 2 cut(s) 150, 540
FblI GTMKAC 3 cut(s) 58, 210, 281
FriOI GRGCYC 3 cut(s) 122, 650, 775
GlaI GCGC 3 cut(s) 249, 597, 909
GsuI CTGGAG 3 cut(s) 188, 413, 829
HaeIII GGCC 9 cut(s) 29, 89, 143, 485, 747, 788, 803, 842, 955
HgaI GACGC 1 cut(s) 29
HhaI GCGC 3 cut(s) 250, 598, 910
Hin1I GRCGYC 2 cut(s) 40, 324
Hin1II CATG 3 cut(s) 403, 646, 745
Hin6I GCGC 3 cut(s) 248, 596, 908
HinP1I GCGC 3 cut(s) 248, 596, 908
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 6 cut(s) 55, 278, 532, 553, 579, 973
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 4 cut(s) 59, 211, 282, 511
Hpy188I TCNGA 5 cut(s) 139, 163, 454, 578, 972
Hpy188III TCNNGA 4 cut(s) 47, 538, 562, 846
Hpy8I GTNNAC 4 cut(s) 59, 211, 282, 511
Hpy99I CGWCG 7 cut(s) 45, 278, 326, 329, 344, 692, 841
HpyAV CCTTC 2 cut(s) 247, 537
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 3 cut(s) 80, 324, 339
HpyCH4V TGCA 4 cut(s) 766, 880, 922, 979
HpyF3I CTNAG 5 cut(s) 53, 774, 816, 825, 1014
HpySE526I ACGT 3 cut(s) 80, 324, 339
Hsp92I GRCGYC 2 cut(s) 40, 324
Hsp92II CATG 3 cut(s) 403, 646, 745
HspAI GCGC 3 cut(s) 248, 596, 908
KroI GCCGGC 2 cut(s) 197, 698
KroNI GCCGGC 2 cut(s) 199, 700
Ksp22I TGATCA 2 cut(s) 150, 540
Kzo9I GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
LmnI GCTCC 6 cut(s) 130, 169, 189, 194, 711, 835
Lsp1109I GCAGC 4 cut(s) 375, 778, 922, 934
LweI GCATC 2 cut(s) 54, 735
MaeII ACGT 3 cut(s) 80, 324, 339
MaeIII GTNAC 1 cut(s) 614
MalI GATC 9 cut(s) 152, 296, 308, 380, 470, 542, 566, 659, 669
MbiI CCGCTC 2 cut(s) 172, 192
MboI GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
MboII GAAGA 1 cut(s) 977
MflI RGATCY 4 cut(s) 294, 306, 378, 468
MhlI GDGCHC 3 cut(s) 122, 650, 775
MlyI GAGTC 3 cut(s) 64, 287, 588
MmeI TCCRAC 1 cut(s) 477
MreI CGCCGGCG 1 cut(s) 698
MroNI GCCGGC 2 cut(s) 197, 698
MseI TTAA 1 cut(s) 794
MslI CAYNNNNRTG 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 769
MspR9I CCNGG 9 cut(s) 18, 111, 133, 235, 286, 383, 550, 650, 716
MvaI CCWGG 2 cut(s) 383, 716
MvnI CGCG 4 cut(s) 248, 303, 321, 516
NaeI GCCGGC 2 cut(s) 199, 700
NciI CCSGG 7 cut(s) 18, 111, 133, 235, 286, 550, 650
NdeII GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
NgoMIV GCCGGC 2 cut(s) 197, 698
NlaIII CATG 3 cut(s) 403, 646, 745
NlaIV GGNNCC 9 cut(s) 114, 126, 136, 196, 290, 609, 631, 713, 831
NmeAIII GCCGAG 2 cut(s) 93, 101
NspV TTCGAA 1 cut(s) 530
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 513
PdiI GCCGGC 2 cut(s) 199, 700
PfeI GAWTC 3 cut(s) 532, 553, 973
PflFI GACNNNGTC 1 cut(s) 39
PleI GAGTC 3 cut(s) 63, 286, 587
PpsI GAGTC 3 cut(s) 63, 286, 587
PshAI GACNNNNGTC 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 381, 714
PspGI CCWGG 2 cut(s) 381, 714
PspN4I GGNNCC 9 cut(s) 114, 126, 136, 196, 290, 609, 631, 713, 831
PspPI GGNCC 8 cut(s) 113, 135, 607, 630, 745, 786, 801, 953
PstNI CAGNNNCTG 2 cut(s) 388, 976
PsuI RGATCY 4 cut(s) 294, 306, 378, 468
PsyI GACNNNGTC 1 cut(s) 39
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 809
SacI GAGCTC 1 cut(s) 122
SalI GTCGAC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 794
Sau3AI GATC 9 cut(s) 150, 294, 306, 378, 468, 540, 564, 657, 667
Sau96I GGNCC 8 cut(s) 113, 135, 607, 630, 745, 786, 801, 953
SchI GAGTC 3 cut(s) 64, 287, 588
ScrFI CCNGG 9 cut(s) 18, 111, 133, 235, 286, 383, 550, 650, 716
SduI GDGCHC 3 cut(s) 122, 650, 775
SfaNI GCATC 2 cut(s) 54, 735
SfcI CTRYAG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 530
SgrAI CRCCGGYG 1 cut(s) 698
SinI GGWCC 4 cut(s) 113, 135, 607, 630
SmiMI CAYNNNNRTG 1 cut(s) 809
SstI GAGCTC 1 cut(s) 122
StyD4I CCNGG 9 cut(s) 16, 109, 131, 233, 284, 381, 548, 648, 714
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 3 cut(s) 83, 327, 342
TaqI TCGA 3 cut(s) 281, 327, 530
TaqII GACCGA 1 cut(s) 130
TfiI GAWTC 3 cut(s) 532, 553, 973
Tru1I TTAA 1 cut(s) 794
Tru9I TTAA 1 cut(s) 794
TseI GCWGC 4 cut(s) 388, 766, 910, 922
TspDTI ATGAA 3 cut(s) 388, 853, 956
TspGWI ACGGA 2 cut(s) 117, 744
Tth111I GACNNNGTC 1 cut(s) 39
VpaK11BI GGWCC 4 cut(s) 113, 135, 607, 630
XmiI GTMKAC 3 cut(s) 58, 210, 281
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.