FvH4_4g27851

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001741
Physical Location & Seq
Forward (+)
28667253 .. 28668397
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27851.t1

Sequence Viewer

Length: 480 bp
ATGAAAAGACTTGGCCGGAACCTCGGACTCCGGTCACCGGCCGGCGGATTCCGGTCACTGGAATTTCCCCGACTGCCGGATTCCGGTCACCGGATTTTCCTCGACCGCCGGATTCTGGTCACCGGATTTTTCCCTAAACACACCTCGCTAGAATATACAGACATGAGTCTACCGATGCCGAGGCGTGACACATCTCTGGCGGTGCCGTTGCCTCTGCCGCTGGGGCCGAGCTCGACGCCGTCCGACTCAGAGGCAGTGATGATCAACTTCGCCGACCTGGATCGGGCCAATCGGATTGAGAGCGGCGCCGGCGGCACAGTCTACAAGGTGATTCACAAGCCGACGGGGCGGGTCTACATGCTGAAAGTGATCTACGACACGCACGATGAGTCGGCGCAGCACCAAATCTTCCGCGAGATCGAAATCCTTCGCGTGGTCTGGTGGTCGAAGAGGACGTTGGAGTGGAGGAGGCCGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

160

Amino Acids

18.17

Weight (kDa)

10.13

Isoelectric Point (pI)

67.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 98 - 145 7.2e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 202, 305
AccBSI CCGCTC 1 cut(s) 303
AccI GTMKAC 3 cut(s) 169, 321, 354
AccII CGCG 2 cut(s) 414, 432
AciI CCGC 8 cut(s) 45, 106, 200, 218, 303, 312, 349, 412
AclWI GGATC 1 cut(s) 288
AcoI YGGCCR 2 cut(s) 13, 39
AcsI RAATTY 1 cut(s) 62
AcyI GRCGYC 2 cut(s) 236, 306
AfiI CCNNNNNNNGG 9 cut(s) 37, 44, 58, 76, 83, 90, 115, 283, 433
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 1 cut(s) 231
AluI AGCT 1 cut(s) 231
Alw21I GWGCWC 1 cut(s) 233
AlwI GGATC 1 cut(s) 288
AoxI GGCC 5 cut(s) 13, 39, 224, 285, 470
ApeKI GCWGC 1 cut(s) 397
ApoI RAATTY 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 426
AspLEI GCGC 2 cut(s) 308, 397
AspS9I GGNCC 2 cut(s) 224, 285
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 4 cut(s) 27, 80, 112, 340
BanI GGYRCC 2 cut(s) 202, 305
BanII GRGCYC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 409
BceAI ACGGC 2 cut(s) 190, 223
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 1 cut(s) 474
BfaI CTAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 309
BglI GCCNNNNNGGC 2 cut(s) 223, 346
BisI GCNGC 4 cut(s) 218, 304, 313, 398
BlsI GCNGC 4 cut(s) 219, 305, 314, 399
Bme1390I CCNGG 2 cut(s) 278, 474
BmgT120I GGNCC 2 cut(s) 224, 285
BmiI GGNNCC 4 cut(s) 20, 204, 225, 307
BmrFI CCNGG 2 cut(s) 278, 474
BmsI GCATC 1 cut(s) 165
BoxI GACNNNNGTC 2 cut(s) 31, 165
BpuMI CCSGG 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 422
BsaHI GRCGYC 2 cut(s) 236, 306
BsaJI CCNNGG 3 cut(s) 22, 179, 473
BsaWI WCCGGW 5 cut(s) 30, 51, 83, 90, 122
Bsc4I CCNNNNNNNGG 9 cut(s) 37, 44, 58, 76, 83, 90, 115, 283, 433
Bse118I RCCGGY 3 cut(s) 37, 41, 308
Bse1I ACTGG 1 cut(s) 63
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 22, 179, 473
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 9 cut(s) 37, 44, 58, 76, 83, 90, 115, 283, 433
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 63
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 220
Bsh1236I CGCG 2 cut(s) 414, 432
Bsh1285I CGRYCG 2 cut(s) 42, 106
BshFI GGCC 5 cut(s) 15, 41, 226, 287, 472
BshNI GGYRCC 2 cut(s) 202, 305
BsiEI CGRYCG 2 cut(s) 42, 106
BsiHKAI GWGCWC 1 cut(s) 233
BslI CCNNNNNNNGG 9 cut(s) 37, 44, 58, 76, 83, 90, 115, 283, 433
BsnI GGCC 5 cut(s) 15, 41, 226, 287, 472
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 4 cut(s) 261, 280, 369, 417
BspACI CCGC 8 cut(s) 45, 106, 200, 218, 303, 312, 349, 412
BspANI GGCC 5 cut(s) 15, 41, 226, 287, 472
BspCNI CTCAG 1 cut(s) 260
BspFNI CGCG 2 cut(s) 414, 432
BspLI GGNNCC 4 cut(s) 20, 204, 225, 307
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 2 cut(s) 202, 305
BsrBI CCGCTC 1 cut(s) 303
BsrFI RCCGGY 3 cut(s) 37, 41, 308
BsrI ACTGG 1 cut(s) 63
BssAI RCCGGY 3 cut(s) 37, 41, 308
BssECI CCNNGG 3 cut(s) 22, 179, 473
BssMI GATC 4 cut(s) 261, 280, 369, 417
BssNI GRCGYC 2 cut(s) 236, 306
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 2 cut(s) 236, 306
BstC8I GCNNGC 2 cut(s) 43, 310
BstDEI CTNAG 1 cut(s) 247
BstEII GGTNACC 3 cut(s) 33, 86, 118
BstFNI CGCG 2 cut(s) 414, 432
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 2 cut(s) 308, 397
BstKTI GATC 4 cut(s) 264, 283, 372, 420
BstMBI GATC 4 cut(s) 261, 280, 369, 417
BstMCI CGRYCG 2 cut(s) 42, 106
BstMWI GCNNNNNNNGC 5 cut(s) 217, 223, 309, 312, 346
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 361
BstPAI GACNNNNGTC 2 cut(s) 31, 165
BstPI GGTNACC 3 cut(s) 33, 86, 118
BstSCI CCNGG 2 cut(s) 276, 472
BstUI CGCG 2 cut(s) 414, 432
BstV1I GCAGC 1 cut(s) 409
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 5 cut(s) 15, 41, 226, 287, 472
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 56, 261
Cac8I GCNNGC 2 cut(s) 43, 310
CfoI GCGC 2 cut(s) 308, 397
Cfr10I RCCGGY 3 cut(s) 37, 41, 308
Cfr13I GGNCC 2 cut(s) 224, 285
CseI GACGC 1 cut(s) 244
CviAII CATG 2 cut(s) 163, 358
CviJI RGCY 7 cut(s) 15, 41, 226, 231, 287, 340, 472
CviKI_1 RGCY 7 cut(s) 15, 41, 226, 231, 287, 340, 472
DdeI CTNAG 1 cut(s) 247
DinI GGCGCC 1 cut(s) 307
DpnI GATC 4 cut(s) 263, 282, 371, 419
DpnII GATC 4 cut(s) 261, 280, 369, 417
EaeI YGGCCR 2 cut(s) 13, 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 231
EclXI CGGCCG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 233
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 231
Eco91I GGTNACC 3 cut(s) 33, 86, 118
EcoICRI GAGCTC 1 cut(s) 231
EcoO65I GGTNACC 3 cut(s) 33, 86, 118
EcoRII CCWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 233
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
FaeI CATG 2 cut(s) 166, 361
FaiI YATR 3 cut(s) 156, 164, 359
FatI CATG 2 cut(s) 162, 357
FauI CCCGC 1 cut(s) 342
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 3 cut(s) 169, 321, 354
Fnu4HI GCNGC 4 cut(s) 218, 304, 313, 398
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 4 cut(s) 218, 304, 313, 398
FspBI CTAG 1 cut(s) 149
GlaI GCGC 2 cut(s) 307, 396
GluI GCNGC 4 cut(s) 218, 304, 313, 398
GsaI CCCAGC 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 5 cut(s) 15, 41, 226, 287, 472
HgaI GACGC 1 cut(s) 244
HhaI GCGC 2 cut(s) 308, 397
Hin1I GRCGYC 2 cut(s) 236, 306
Hin1II CATG 2 cut(s) 166, 361
Hin6I GCGC 2 cut(s) 306, 395
HinP1I GCGC 2 cut(s) 306, 395
HinfI GANTC 8 cut(s) 27, 48, 80, 112, 166, 245, 331, 389
HphI GGTGA 4 cut(s) 27, 80, 112, 340
Hpy166II GTNNAC 3 cut(s) 170, 322, 355
Hpy188I TCNGA 4 cut(s) 26, 244, 250, 294
Hpy8I GTNNAC 3 cut(s) 170, 322, 355
Hpy99I CGWCG 2 cut(s) 238, 346
HpyAV CCTTC 1 cut(s) 437
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 455
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 223, 309, 312, 346
HpyF3I CTNAG 1 cut(s) 247
HpySE526I ACGT 1 cut(s) 455
Hsp92I GRCGYC 2 cut(s) 236, 306
Hsp92II CATG 2 cut(s) 166, 361
HspAI GCGC 2 cut(s) 306, 395
KasI GGCGCC 1 cut(s) 305
KroI GCCGGC 2 cut(s) 41, 308
KroNI GCCGGC 2 cut(s) 43, 310
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 4 cut(s) 261, 280, 369, 417
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 149
MaeII ACGT 1 cut(s) 455
MaeIII GTNAC 5 cut(s) 33, 54, 86, 118, 185
MalI GATC 4 cut(s) 263, 282, 371, 419
MbiI CCGCTC 1 cut(s) 303
MboI GATC 4 cut(s) 261, 280, 369, 417
MboII GAAGA 2 cut(s) 400, 460
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 1 cut(s) 62
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 4 cut(s) 21, 175, 239, 398
MmeI TCCRAC 2 cut(s) 267, 438
MnlI CCTC 9 cut(s) 32, 110, 154, 174, 222, 244, 444, 459, 462
MreI CGCCGGCG 1 cut(s) 308
MroNI GCCGGC 2 cut(s) 41, 308
MroXI GAANNNNTTC 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 220
MspR9I CCNGG 2 cut(s) 278, 474
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 2 cut(s) 414, 432
MwoI GCNNNNNNNGC 5 cut(s) 217, 223, 309, 312, 346
NaeI GCCGGC 2 cut(s) 43, 310
NarI GGCGCC 1 cut(s) 306
NciI CCSGG 1 cut(s) 474
NdeII GATC 4 cut(s) 261, 280, 369, 417
NgoMIV GCCGGC 2 cut(s) 41, 308
NlaIII CATG 2 cut(s) 166, 361
NlaIV GGNNCC 4 cut(s) 20, 204, 225, 307
NmeAIII GCCGAG 2 cut(s) 204, 252
NmuCI GTSAC 5 cut(s) 33, 54, 86, 118, 185
NspI RCATGY 1 cut(s) 361
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 452
PdiI GCCGGC 2 cut(s) 43, 310
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 4 cut(s) 48, 80, 112, 331
PflFI GACNNNGTC 1 cut(s) 238
PkrI GCNGC 4 cut(s) 219, 305, 314, 399
PleI GAGTC 4 cut(s) 21, 174, 239, 397
PluTI GGCGCC 1 cut(s) 309
PpsI GAGTC 4 cut(s) 21, 174, 239, 397
PshAI GACNNNNGTC 2 cut(s) 31, 165
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 276
PspEI GGTNACC 3 cut(s) 33, 86, 118
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 4 cut(s) 20, 204, 225, 307
PspPI GGNCC 2 cut(s) 224, 285
PsyI GACNNNGTC 1 cut(s) 238
SacI GAGCTC 1 cut(s) 233
SatI GCNGC 4 cut(s) 218, 304, 313, 398
Sau3AI GATC 4 cut(s) 261, 280, 369, 417
Sau96I GGNCC 2 cut(s) 224, 285
SchI GAGTC 4 cut(s) 21, 175, 239, 398
ScrFI CCNGG 2 cut(s) 278, 474
SduI GDGCHC 1 cut(s) 233
SetI ASST 6 cut(s) 24, 146, 233, 279, 330, 458
SfaNI GCATC 1 cut(s) 165
SfoI GGCGCC 1 cut(s) 307
SgrAI CRCCGGYG 1 cut(s) 308
Sse9I AATT 1 cut(s) 62
SsiI CCGC 8 cut(s) 45, 106, 200, 218, 303, 312, 349, 412
SspDI GGCGCC 1 cut(s) 305
SspMI CTAG 1 cut(s) 149
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 276, 472
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 458
TaqI TCGA 4 cut(s) 102, 233, 420, 446
TasI AATT 1 cut(s) 62
TauI GCSGC 3 cut(s) 220, 306, 315
TfiI GAWTC 4 cut(s) 48, 80, 112, 331
TscAI CASTG 2 cut(s) 63, 261
TseFI GTSAC 5 cut(s) 33, 54, 86, 118, 185
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 5 cut(s) 33, 54, 86, 118, 185
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 63, 261
Tth111I GACNNNGTC 1 cut(s) 238
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 361
XmiI GTMKAC 3 cut(s) 169, 321, 354
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.