Rw2G039170

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001741
Physical Location & Seq
Forward (+)
64122544 .. 64124085
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039170.1

Sequence Viewer

Length: 1104 bp
ATGTCGACGAGAAGTATTTGCCAATCACCTTCAAGTTCTAAACGTCAACACTGGACAAAGCAGCTTCGTAACAAGATTCGTAGCATGTTGTCGATGCACCAACGTTCCATACCGCAGCCTCCCCCTCCGGTTCCCACCTCGGTTCCGACGGAGGAACTTCCCATCAACTTCTTGGAGCCAGAGCGGGCAATCAACTCCTATCTCAATCAGGGCAGCCTCAACCTCCCCCTCCGGTTCCATACCGCAGCCTCCGCCTCCGTACCGGAGTCACTTCTGATCAACTTCTCGGATATAGAGCGGGAGAAAAAACCGATTAGAAGCGGAGCCGGAGGCACGGTCTACAAAGCCGTTCACAAGCCGACCGGCCTGCTCTACGCGGTGAAAGTGATCTACGGAGACCCTGAGGACTCGGTCCGGCGTCAGATCTTCGAAGAGTTCCAAGTCCTCCGCAAAGTTGCTAACCCTAACGTCGTCAAGTGCTACGACATTTTCGATCACAACAGCGAGATACAGGTGCTGCTAGAATTCATGGACGGAGGTTCGTTAAAGGGCAAGTACATCCACGACGAGAAGGCTCTCTCCAATTTGGCTCGGCAGATCCTCACCGGCTTGGCCTATCTCCACAGACGGCACGTCGTCCACCGCGATATCAAGCCCGCTAATCTCTTGATCAACACCCGAAATGAAGTCAAAATTGCTGATTTCGTGGGAAAAGTTTTGGCGCGGACTATGGACCCTTGTAACTCTTGTGTGGGAAATATAGCCTACATGAGCCCGGAGAGGATCAACCCTGATCTCAATCAGGGCAGTTACGACAGCTACGCCGGCGATATTTGGAGCTTTGGGGTCAGCATCTTGGAAATTTACGTAGGTCGGTACCCCTTCTCTGTGGATCGTTACCAAAAAGGATACTGGTTCCGCTTAACTTGGGTAATATGTATGTCTCAGCCTCCTGAAGCTCCTTCTACTGCGTCCAGTGAGTTCAGGCATTTCATTGCTTGCTGTTTGCAACGAGAGCCGAATAAAAGATTCTCGGCGGCTCAGTTGTTGCAGCACCCATTCATCTCTGGCAATGGCGGTGGACAGACCCAAGTTGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

367

Amino Acids

41.49

Weight (kDa)

9.14

Isoelectric Point (pI)

59.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 102 - 351 2.2e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 103 - 355 1.2e-59 Protein kinase domain
Kinase-like PF14531 189 - 300 1.5e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 876
AccB1I GGYRCC 1 cut(s) 876
AccBSI CCGCTC 2 cut(s) 184, 298
AccI GTMKAC 2 cut(s) 5, 339
AccII CGCG 3 cut(s) 377, 645, 724
AclI AACGTT 1 cut(s) 103
AclWI GGATC 3 cut(s) 592, 791, 900
AcsI RAATTY 2 cut(s) 524, 861
AcuI CTGAAG 1 cut(s) 975
AcyI GRCGYC 1 cut(s) 418
AfaI GTAC 3 cut(s) 261, 557, 878
AgsI TTSAA 1 cut(s) 33
AhdI GACNNNNNGTC 1 cut(s) 632
AjiI CACGTC 1 cut(s) 634
AluBI AGCT 4 cut(s) 64, 819, 840, 959
AluI AGCT 4 cut(s) 64, 819, 840, 959
Alw26I GTCTC 2 cut(s) 390, 948
AlwI GGATC 3 cut(s) 592, 791, 900
AlwNI CAGNNNCTG 1 cut(s) 517
AoxI GGCC 2 cut(s) 364, 612
ApeKI GCWGC 6 cut(s) 61, 115, 213, 245, 517, 1051
ApoI RAATTY 2 cut(s) 524, 861
Asp718I GGTACC 1 cut(s) 876
AspLEI GCGC 1 cut(s) 724
AspS9I GGNCC 2 cut(s) 412, 733
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 3 cut(s) 18, 391, 595
AsuII TTCGAA 1 cut(s) 429
AvaII GGWCC 2 cut(s) 412, 733
AxyI CCTNAGG 1 cut(s) 402
BanI GGYRCC 1 cut(s) 876
BanII GRGCYC 1 cut(s) 776
BbvI GCAGC 6 cut(s) 73, 127, 225, 257, 504, 1063
BccI CCATC 1 cut(s) 170
BceAI ACGGC 2 cut(s) 332, 644
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciVI GTATCC 1 cut(s) 902
BclI TGATCA 3 cut(s) 276, 669, 1096
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 2 cut(s) 390, 948
BfaI CTAG 1 cut(s) 521
BfuI GTATCC 1 cut(s) 902
BglII AGATCT 1 cut(s) 423
BisI GCNGC 7 cut(s) 62, 116, 214, 246, 518, 1038, 1052
BlsI GCNGC 7 cut(s) 63, 117, 215, 247, 519, 1039, 1053
Bme1390I CCNGG 1 cut(s) 776
Bme18I GGWCC 2 cut(s) 412, 733
BmeRI GACNNNNNGTC 1 cut(s) 632
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 2 cut(s) 412, 733
BmiI GGNNCC 8 cut(s) 132, 144, 177, 236, 325, 735, 878, 917
BmrFI CCNGG 1 cut(s) 776
BmsI GCATC 2 cut(s) 84, 861
Bpu14I TTCGAA 1 cut(s) 429
BpuMI CCSGG 1 cut(s) 776
BsaAI YACGTR 1 cut(s) 868
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 418
BsaI GGTCTC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 3 cut(s) 127, 231, 262
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bse118I RCCGGY 3 cut(s) 362, 605, 824
Bse1I ACTGG 3 cut(s) 56, 917, 975
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 2 cut(s) 993, 1078
Bse8I GATNNNNATC 1 cut(s) 798
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 558
BseJI GATNNNNATC 1 cut(s) 798
BseMI GCAATG 2 cut(s) 993, 1078
BseMII CTCAG 3 cut(s) 393, 959, 1055
BseNI ACTGG 3 cut(s) 56, 917, 975
BseXI GCAGC 6 cut(s) 73, 127, 225, 257, 504, 1063
Bsh1236I CGCG 3 cut(s) 377, 645, 724
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 2 cut(s) 366, 614
BshNI GGYRCC 1 cut(s) 876
BsiEI CGRYCG 1 cut(s) 363
BsiSI CCGG 9 cut(s) 128, 232, 263, 327, 363, 415, 606, 776, 825
BsmAI GTCTC 2 cut(s) 390, 948
BsnI GGCC 2 cut(s) 366, 614
Bso31I GGTCTC 1 cut(s) 390
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 776
BspANI GGCC 2 cut(s) 366, 614
BspCNI CTCAG 3 cut(s) 394, 958, 1054
BspFNI CGCG 3 cut(s) 377, 645, 724
BspLI GGNNCC 8 cut(s) 132, 144, 177, 236, 325, 735, 878, 917
BspPI GGATC 3 cut(s) 592, 791, 900
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 876
BspTNI GGTCTC 1 cut(s) 390
BsrBI CCGCTC 2 cut(s) 184, 298
BsrDI GCAATG 2 cut(s) 993, 1078
BsrFI RCCGGY 3 cut(s) 362, 605, 824
BsrI ACTGG 3 cut(s) 56, 917, 975
BssAI RCCGGY 3 cut(s) 362, 605, 824
BssECI CCNNGG 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 337
Bst6I CTCTTC 1 cut(s) 426
BstACI GRCGYC 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 868
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 5 cut(s) 186, 368, 657, 826, 1000
BstDEI CTNAG 3 cut(s) 402, 945, 1041
BstF5I GGATG 1 cut(s) 558
BstFNI CGCG 3 cut(s) 377, 645, 724
BstHHI GCGC 1 cut(s) 724
BstMAI GTCTC 2 cut(s) 390, 948
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 3 cut(s) 251, 825, 1015
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 774
BstSNI TACGTA 1 cut(s) 868
BstUI CGCG 3 cut(s) 377, 645, 724
BstV1I GCAGC 6 cut(s) 73, 127, 225, 257, 504, 1063
BstX2I RGATCY 2 cut(s) 423, 597
BstYI RGATCY 2 cut(s) 423, 597
Bsu36I CCTNAGG 1 cut(s) 402
BsuI GTATCC 1 cut(s) 902
BsuRI GGCC 2 cut(s) 366, 614
BtrI CACGTC 1 cut(s) 634
BtsCI GGATG 1 cut(s) 558
BtsIMutI CAGTG 2 cut(s) 49, 982
Cac8I GCNNGC 5 cut(s) 186, 368, 657, 826, 1000
CaiI CAGNNNCTG 1 cut(s) 517
CfoI GCGC 1 cut(s) 724
Cfr10I RCCGGY 3 cut(s) 362, 605, 824
Cfr13I GGNCC 2 cut(s) 412, 733
CpoI CGGWCCG 1 cut(s) 412
CseI GACGC 2 cut(s) 407, 960
Csp6I GTAC 3 cut(s) 260, 556, 877
CspCI CAANNNNNGTGG 2 cut(s) 1060, 1095
CspI CGGWCCG 1 cut(s) 412
CviAII CATG 3 cut(s) 85, 529, 769
CviQI GTAC 3 cut(s) 260, 556, 877
DdeI CTNAG 3 cut(s) 402, 945, 1041
DriI GACNNNNNGTC 1 cut(s) 632
Eam1104I CTCTTC 1 cut(s) 426
Eam1105I GACNNNNNGTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 426
EciI GGCGGA 1 cut(s) 241
Eco105I TACGTA 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 776
Eco31I GGTCTC 1 cut(s) 390
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 2 cut(s) 412, 733
Eco57I CTGAAG 1 cut(s) 975
Eco81I CCTNAGG 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 524
EcoRV GATATC 1 cut(s) 649
EcoT38I GRGCYC 1 cut(s) 776
FaeI CATG 3 cut(s) 88, 532, 772
FatI CATG 3 cut(s) 84, 528, 768
FauI CCCGC 3 cut(s) 177, 291, 664
FbaI TGATCA 3 cut(s) 276, 669, 1096
FblI GTMKAC 2 cut(s) 5, 339
Fnu4HI GCNGC 7 cut(s) 62, 116, 214, 246, 518, 1038, 1052
FokI GGATG 1 cut(s) 545
FriOI GRGCYC 1 cut(s) 776
Fsp4HI GCNGC 7 cut(s) 62, 116, 214, 246, 518, 1038, 1052
FspBI CTAG 1 cut(s) 521
GlaI GCGC 1 cut(s) 723
GluI GCNGC 7 cut(s) 62, 116, 214, 246, 518, 1038, 1052
HaeIII GGCC 2 cut(s) 366, 614
HapII CCGG 9 cut(s) 128, 232, 263, 327, 363, 415, 606, 776, 825
HgaI GACGC 2 cut(s) 407, 960
HhaI GCGC 1 cut(s) 724
Hin1I GRCGYC 1 cut(s) 418
Hin1II CATG 3 cut(s) 88, 532, 772
Hin6I GCGC 1 cut(s) 722
HinP1I GCGC 1 cut(s) 722
HincII GTYRAC 2 cut(s) 6, 47
HindII GTYRAC 2 cut(s) 6, 47
HinfI GANTC 4 cut(s) 76, 266, 407, 1029
HpaII CCGG 9 cut(s) 128, 232, 263, 327, 363, 415, 606, 776, 825
HphI GGTGA 3 cut(s) 18, 391, 595
Hpy166II GTNNAC 6 cut(s) 6, 47, 340, 352, 640, 1082
Hpy188I TCNGA 4 cut(s) 147, 276, 289, 423
Hpy188III TCNNGA 2 cut(s) 667, 953
Hpy8I GTNNAC 6 cut(s) 6, 47, 340, 352, 640, 1082
Hpy99I CGWCG 5 cut(s) 10, 151, 473, 569, 638
HpyAV CCTTC 4 cut(s) 39, 565, 892, 972
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 5 cut(s) 43, 103, 468, 633, 867
HpyCH4V TGCA 3 cut(s) 97, 1009, 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 825, 1015
HpyF3I CTNAG 3 cut(s) 402, 945, 1041
HpySE526I ACGT 5 cut(s) 43, 103, 468, 633, 867
Hsp92I GRCGYC 1 cut(s) 418
Hsp92II CATG 3 cut(s) 88, 532, 772
HspAI GCGC 1 cut(s) 722
KpnI GGTACC 1 cut(s) 880
KroI GCCGGC 1 cut(s) 824
KroNI GCCGGC 1 cut(s) 826
Ksp22I TGATCA 3 cut(s) 276, 669, 1096
LmnI GCTCC 4 cut(s) 175, 323, 837, 964
Lsp1109I GCAGC 6 cut(s) 73, 127, 225, 257, 504, 1063
LweI GCATC 2 cut(s) 84, 861
MaeI CTAG 1 cut(s) 521
MaeII ACGT 5 cut(s) 43, 103, 468, 633, 867
MaeIII GTNAC 5 cut(s) 68, 267, 740, 809, 896
MbiI CCGCTC 2 cut(s) 184, 298
MboII GAAGA 2 cut(s) 418, 443
MflI RGATCY 2 cut(s) 423, 597
MhlI GDGCHC 1 cut(s) 776
MluCI AATT 4 cut(s) 524, 583, 693, 861
MlyI GAGTC 2 cut(s) 275, 401
MmeI TCCRAC 1 cut(s) 170
MreI CGCCGGCG 1 cut(s) 824
MroNI GCCGGC 1 cut(s) 824
MseI TTAA 2 cut(s) 545, 923
MspI CCGG 9 cut(s) 128, 232, 263, 327, 363, 415, 606, 776, 825
MspR9I CCNGG 1 cut(s) 776
MvnI CGCG 3 cut(s) 377, 645, 724
MwoI GCNNNNNNNGC 3 cut(s) 251, 825, 1015
NaeI GCCGGC 1 cut(s) 826
NciI CCSGG 1 cut(s) 776
NgoMIV GCCGGC 1 cut(s) 824
NlaIII CATG 3 cut(s) 88, 532, 772
NlaIV GGNNCC 8 cut(s) 132, 144, 177, 236, 325, 735, 878, 917
NmeAIII GCCGAG 2 cut(s) 571, 1013
NmuCI GTSAC 1 cut(s) 267
NspI RCATGY 1 cut(s) 88
NspV TTCGAA 1 cut(s) 429
PcsI WCGNNNNNNNCGW 2 cut(s) 489, 642
PdiI GCCGGC 1 cut(s) 826
PfeI GAWTC 2 cut(s) 76, 1029
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 7 cut(s) 63, 117, 215, 247, 519, 1039, 1053
PleI GAGTC 2 cut(s) 274, 401
PpsI GAGTC 2 cut(s) 274, 401
Ppu21I YACGTR 1 cut(s) 868
Psp1406I AACGTT 1 cut(s) 103
PspN4I GGNNCC 8 cut(s) 132, 144, 177, 236, 325, 735, 878, 917
PspPI GGNCC 2 cut(s) 412, 733
PstNI CAGNNNCTG 1 cut(s) 517
PsuI RGATCY 2 cut(s) 423, 597
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 3 cut(s) 261, 557, 878
RsaNI GTAC 3 cut(s) 260, 556, 877
Rsr2I CGGWCCG 1 cut(s) 412
RsrII CGGWCCG 1 cut(s) 412
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 545, 923
SatI GCNGC 7 cut(s) 62, 116, 214, 246, 518, 1038, 1052
Sau96I GGNCC 2 cut(s) 412, 733
SchI GAGTC 2 cut(s) 275, 401
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 2 cut(s) 84, 861
SfuI TTCGAA 1 cut(s) 429
SgrAI CRCCGGYG 1 cut(s) 824
SinI GGWCC 2 cut(s) 412, 733
SnaBI TACGTA 1 cut(s) 868
Sse9I AATT 4 cut(s) 524, 583, 693, 861
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 774
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 5 cut(s) 46, 106, 471, 636, 870
TaqI TCGA 4 cut(s) 5, 92, 429, 492
TaqII GACCGA 1 cut(s) 400
TasI AATT 4 cut(s) 524, 583, 693, 861
TatI WGTACW 1 cut(s) 555
TauI GCSGC 1 cut(s) 1040
TfiI GAWTC 2 cut(s) 76, 1029
Tru1I TTAA 2 cut(s) 545, 923
Tru9I TTAA 2 cut(s) 545, 923
TscAI CASTG 2 cut(s) 56, 982
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 6 cut(s) 61, 115, 213, 245, 517, 1051
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 4 cut(s) 517, 699, 982, 1051
TspGWI ACGGA 4 cut(s) 164, 247, 408, 549
TspRI CASTG 2 cut(s) 56, 982
Tth111I GACNNNGTC 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 412, 733
XapI RAATTY 2 cut(s) 524, 861
XceI RCATGY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 5, 339
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.