RchiOBHm_Chr2g0150331

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001741
Physical Location & Seq
Forward (+)
68021409 .. 68023644
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51967

Sequence Viewer

Length: 1062 bp
ATGAGACCGAATAATATGAGTCAGATGCCGCCGCCGCCGCCGGGAGTTGTGGCCGCCAGAAAGGACCCGTCGGGGAAGACACGGCGTCGTCCTGACCTGAGTCTACCGATGCCGAGGCGGGACACGTCTCTGGCCGTGCCGTTGCCTCTGCCGCCGGGACCGAGCTCGGCTCCGTCCGGGTCCGAGGCCGTGATGATCAACTTCTCCGAGCTGGAGCGGGCGAACCGGATTGGGAGCGGAGCGGGCGGCACGGTCTACAAGGTTATTCACAAGCCGACGGGGCGGCTCTACGCGCTGAAAGTGATCTACGGCAACCACGACGAGTCGACCCGGCACCAGATCTGCCGCGAGATCCAAATCCTCCGCGACGTCGATAACCCTAACGTCGTCAAGTGCCACGATATGTTTGACCACAACGGCGAGATCCAGGTGCTGCTGGAGTTCATGGACGGCGGTTCGTTAGAGGGGAAGCACATCGCCAACGAGAAAAGCCTCTCCGACTTGGCACGGCAGATCCTCACCGGATTGGCCTACCTCCACAAACGGCACATCGTCCACCGCGACATCAAGCCTTCGAATCTCTTGATCAACGCCCGGAATCAGGTCAAGATCGCCGATTTCGGAGTCAGCCGGATTTTGGCGCAGACTATGGACCCGTGTAACTCCTCTGTAGGGACCATAGCCTACATGAGCCCGGAGAGGATCAACACCGATCTCAATCAGGGCAGGTACGACGGCTACGCCGGCGATATCTGGAGCCTGGGGGTCAGCATCTTGGAGTTTTACATGGGCCGATTTCCGTTCAATGTGCAGCGGGCTCAGGGAGATTGGGCCAGCTTAATGTGGGCCATTTGTATGTCTCAGCCGCCTGAGGCTCCTCCGACGGCCTCCAGAGATTTCCGGCATTTCATTGCTTGCTGTTTGCAGAGAGAGCCGAACAGGAGAATGTCGGCCCAGCAGTTGTTGCAGCACCCATTTATCTCCGGCAATGGGGGCGGCCCGCCGCAGGTTCATCAGAATCTGCACACTCTTCTACCACCGCCACGCCCGCTTCCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

353

Amino Acids

39.16

Weight (kDa)

9.28

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 73 - 327 1.2e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 73 - 323 8.8e-39 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 157 - 272 6.6e-07 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 372
Acc36I ACCTGC 2 cut(s) 717, 997
AccB1I GGYRCC 1 cut(s) 333
AccBSI CCGCTC 3 cut(s) 217, 237, 242
AccI GTMKAC 3 cut(s) 103, 255, 326
AccII CGCG 4 cut(s) 293, 348, 366, 561
AclWI GGATC 4 cut(s) 346, 418, 508, 710
AcoI YGGCCR 2 cut(s) 51, 132
AcyI GRCGYC 2 cut(s) 85, 369
AfaI GTAC 1 cut(s) 731
AfiI CCNNNNNNNGG 6 cut(s) 41, 601, 637, 672, 990, 1006
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 1 cut(s) 805
AhdI GACNNNNNGTC 1 cut(s) 84
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 2 cut(s) 426, 759
AluBI AGCT 3 cut(s) 165, 211, 837
AluI AGCT 3 cut(s) 165, 211, 837
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 2 cut(s) 132, 864
AlwI GGATC 4 cut(s) 346, 418, 508, 710
AlwNI CAGNNNCTG 2 cut(s) 433, 1021
ApeKI GCWGC 3 cut(s) 433, 811, 967
AspLEI GCGC 2 cut(s) 295, 643
AsuC2I CCSGG 6 cut(s) 42, 156, 178, 331, 595, 695
AsuHPI GGTGA 1 cut(s) 511
AsuII TTCGAA 1 cut(s) 575
AvaII GGWCC 5 cut(s) 64, 158, 180, 652, 675
AxyI CCTNAGG 1 cut(s) 870
BanI GGYRCC 1 cut(s) 333
BanII GRGCYC 3 cut(s) 167, 695, 820
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 420, 823, 979
BciT130I CCWGG 2 cut(s) 428, 761
BclI TGATCA 2 cut(s) 195, 585
BcnI CCSGG 6 cut(s) 42, 156, 178, 331, 595, 695
BcoDI GTCTC 2 cut(s) 132, 864
BfmI CTRYAG 1 cut(s) 669
BfuAI ACCTGC 2 cut(s) 717, 997
BglI GCCNNNNNGGC 1 cut(s) 280
BglII AGATCT 1 cut(s) 339
Bme1390I CCNGG 8 cut(s) 42, 156, 178, 331, 428, 595, 695, 761
Bme18I GGWCC 5 cut(s) 64, 158, 180, 652, 675
BmeRI GACNNNNNGTC 1 cut(s) 84
BmgBI CACGTC 1 cut(s) 126
BmiI GGNNCC 9 cut(s) 66, 159, 171, 181, 335, 654, 676, 758, 876
BmrFI CCNGG 8 cut(s) 42, 156, 178, 331, 428, 595, 695, 761
BmsI GCATC 3 cut(s) 15, 99, 780
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 83
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BpmI CTGGAG 4 cut(s) 233, 458, 775, 874
Bpu10I CCTNAGC 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 6 cut(s) 42, 156, 178, 331, 595, 695
BsaBI GATNNNNATC 2 cut(s) 356, 717
BsaHI GRCGYC 2 cut(s) 85, 369
BsaJI CCNNGG 3 cut(s) 113, 183, 760
BsaWI WCCGGW 2 cut(s) 225, 521
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 601, 637, 672, 990, 1006
Bse118I RCCGGY 1 cut(s) 743
Bse21I CCTNAGG 1 cut(s) 870
Bse3DI GCAATG 2 cut(s) 909, 994
Bse8I GATNNNNATC 2 cut(s) 356, 717
BseBI CCWGG 2 cut(s) 428, 761
BseDI CCNNGG 3 cut(s) 113, 183, 760
BseJI GATNNNNATC 2 cut(s) 356, 717
BseLI CCNNNNNNNGG 6 cut(s) 41, 601, 637, 672, 990, 1006
BseMI GCAATG 2 cut(s) 909, 994
BseMII CTCAG 4 cut(s) 89, 833, 861, 875
BseRI GAGGAG 2 cut(s) 655, 867
BseXI GCAGC 3 cut(s) 420, 823, 979
BseYI CCCAGC 1 cut(s) 954
BsgI GTGCAG 2 cut(s) 830, 1007
Bsh1236I CGCG 4 cut(s) 293, 348, 366, 561
BshNI GGYRCC 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 167
BslFI GGGAC 3 cut(s) 134, 171, 688
BslI CCNNNNNNNGG 6 cut(s) 41, 601, 637, 672, 990, 1006
BsmAI GTCTC 2 cut(s) 132, 864
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 3 cut(s) 134, 171, 688
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 3 cut(s) 167, 695, 820
BspCNI CTCAG 4 cut(s) 90, 832, 862, 874
BspFNI CGCG 4 cut(s) 293, 348, 366, 561
BspLI GGNNCC 9 cut(s) 66, 159, 171, 181, 335, 654, 676, 758, 876
BspMI ACCTGC 2 cut(s) 717, 997
BspPI GGATC 4 cut(s) 346, 418, 508, 710
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 333
BsrBI CCGCTC 3 cut(s) 217, 237, 242
BsrDI GCAATG 2 cut(s) 909, 994
BsrFI RCCGGY 1 cut(s) 743
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 3 cut(s) 113, 183, 760
BssNI GRCGYC 2 cut(s) 85, 369
Bst2UI CCWGG 2 cut(s) 428, 761
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 1035
BstACI GRCGYC 2 cut(s) 85, 369
BstAPI GCANNNNNTGC 1 cut(s) 964
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 8 cut(s) 219, 244, 745, 816, 835, 916, 1001, 1049
BstDEI CTNAG 5 cut(s) 98, 819, 861, 870, 1059
BstFNI CGCG 4 cut(s) 293, 348, 366, 561
BstHHI GCGC 2 cut(s) 295, 643
BstMAI GTCTC 2 cut(s) 132, 864
BstNI CCWGG 2 cut(s) 428, 761
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 8 cut(s) 40, 154, 176, 329, 426, 593, 693, 759
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 4 cut(s) 293, 348, 366, 561
BstV1I GCAGC 3 cut(s) 420, 823, 979
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 4 cut(s) 339, 351, 423, 513
BstYI RGATCY 4 cut(s) 339, 351, 423, 513
Bsu36I CCTNAGG 1 cut(s) 870
BtgZI GCGATG 1 cut(s) 460
BtrI CACGTC 1 cut(s) 126
BveI ACCTGC 2 cut(s) 717, 997
Cac8I GCNNGC 8 cut(s) 219, 244, 745, 816, 835, 916, 1001, 1049
CaiI CAGNNNCTG 2 cut(s) 433, 1021
CfoI GCGC 2 cut(s) 295, 643
Cfr10I RCCGGY 1 cut(s) 743
CseI GACGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 730
CviAII CATG 3 cut(s) 445, 688, 787
CviQI GTAC 1 cut(s) 730
DdeI CTNAG 5 cut(s) 98, 819, 861, 870, 1059
DriI GACNNNNNGTC 1 cut(s) 84
EaeI YGGCCR 2 cut(s) 51, 132
Eam1104I CTCTTC 1 cut(s) 1035
Eam1105I GACNNNNNGTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 1035
Ecl136II GAGCTC 1 cut(s) 165
Eco24I GRGCYC 3 cut(s) 167, 695, 820
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 5 cut(s) 64, 158, 180, 652, 675
Eco53kI GAGCTC 1 cut(s) 165
Eco81I CCTNAGG 1 cut(s) 870
EcoICRI GAGCTC 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 426, 759
EcoRV GATATC 1 cut(s) 751
EcoT38I GRGCYC 3 cut(s) 167, 695, 820
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 3 cut(s) 448, 691, 790
FaiI YATR 8 cut(s) 17, 404, 446, 650, 680, 689, 788, 857
FaqI GGGAC 3 cut(s) 134, 171, 688
FatI CATG 3 cut(s) 444, 687, 786
FauI CCCGC 6 cut(s) 111, 210, 235, 807, 1008, 1056
FbaI TGATCA 2 cut(s) 195, 585
FblI GTMKAC 3 cut(s) 103, 255, 326
FriOI GRGCYC 3 cut(s) 167, 695, 820
GlaI GCGC 2 cut(s) 294, 642
GsaI CCCAGC 1 cut(s) 958
GsuI CTGGAG 4 cut(s) 233, 458, 775, 874
HgaI GACGC 1 cut(s) 74
HhaI GCGC 2 cut(s) 295, 643
Hin1I GRCGYC 2 cut(s) 85, 369
Hin1II CATG 3 cut(s) 448, 691, 790
Hin6I GCGC 2 cut(s) 293, 641
HinP1I GCGC 2 cut(s) 293, 641
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HinfI GANTC 7 cut(s) 19, 100, 323, 577, 598, 624, 1018
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 4 cut(s) 104, 256, 327, 556
Hpy188I TCNGA 7 cut(s) 24, 184, 208, 499, 623, 882, 1017
Hpy188III TCNNGA 5 cut(s) 92, 583, 607, 754, 891
Hpy8I GTNNAC 4 cut(s) 104, 256, 327, 556
Hpy99I CGWCG 9 cut(s) 73, 90, 280, 323, 371, 374, 389, 737, 886
HpyAV CCTTC 1 cut(s) 582
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 3 cut(s) 125, 369, 384
HpyCH4V TGCA 4 cut(s) 811, 925, 967, 1024
HpyF3I CTNAG 5 cut(s) 98, 819, 861, 870, 1059
HpySE526I ACGT 3 cut(s) 125, 369, 384
Hsp92I GRCGYC 2 cut(s) 85, 369
Hsp92II CATG 3 cut(s) 448, 691, 790
HspAI GCGC 2 cut(s) 293, 641
KroI GCCGGC 1 cut(s) 743
KroNI GCCGGC 1 cut(s) 745
Ksp22I TGATCA 2 cut(s) 195, 585
LmnI GCTCC 6 cut(s) 175, 214, 234, 239, 756, 880
Lsp1109I GCAGC 3 cut(s) 420, 823, 979
LweI GCATC 3 cut(s) 15, 99, 780
MaeII ACGT 3 cut(s) 125, 369, 384
MaeIII GTNAC 1 cut(s) 659
MbiI CCGCTC 3 cut(s) 217, 237, 242
MboII GAAGA 2 cut(s) 88, 1022
MflI RGATCY 4 cut(s) 339, 351, 423, 513
MhlI GDGCHC 3 cut(s) 167, 695, 820
MlyI GAGTC 4 cut(s) 28, 109, 332, 633
MmeI TCCRAC 2 cut(s) 522, 905
MreI CGCCGGCG 1 cut(s) 743
MroNI GCCGGC 1 cut(s) 743
MseI TTAA 1 cut(s) 839
MslI CAYNNNNRTG 1 cut(s) 854
MspA1I CMGCKG 1 cut(s) 814
MspR9I CCNGG 8 cut(s) 42, 156, 178, 331, 428, 595, 695, 761
MvaI CCWGG 2 cut(s) 428, 761
MvnI CGCG 4 cut(s) 293, 348, 366, 561
NaeI GCCGGC 1 cut(s) 745
NciI CCSGG 6 cut(s) 42, 156, 178, 331, 595, 695
NgoMIV GCCGGC 1 cut(s) 743
NlaIII CATG 3 cut(s) 448, 691, 790
NlaIV GGNNCC 9 cut(s) 66, 159, 171, 181, 335, 654, 676, 758, 876
NmeAIII GCCGAG 2 cut(s) 138, 146
NspV TTCGAA 1 cut(s) 575
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 558
PdiI GCCGGC 1 cut(s) 745
PfeI GAWTC 3 cut(s) 577, 598, 1018
PleI GAGTC 4 cut(s) 27, 108, 331, 632
PpsI GAGTC 4 cut(s) 27, 108, 331, 632
PpuMI RGGWCCY 1 cut(s) 64
PshAI GACNNNNGTC 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 426, 759
PspFI CCCAGC 1 cut(s) 954
PspGI CCWGG 2 cut(s) 426, 759
PspN4I GGNNCC 9 cut(s) 66, 159, 171, 181, 335, 654, 676, 758, 876
PspPPI RGGWCCY 1 cut(s) 64
PstNI CAGNNNCTG 2 cut(s) 433, 1021
PsuI RGATCY 4 cut(s) 339, 351, 423, 513
RsaI GTAC 1 cut(s) 731
RsaNI GTAC 1 cut(s) 730
RseI CAYNNNNRTG 1 cut(s) 854
SacI GAGCTC 1 cut(s) 167
SalI GTCGAC 1 cut(s) 325
SaqAI TTAA 1 cut(s) 839
SchI GAGTC 4 cut(s) 28, 109, 332, 633
ScrFI CCNGG 8 cut(s) 42, 156, 178, 331, 428, 595, 695, 761
SduI GDGCHC 3 cut(s) 167, 695, 820
SfaNI GCATC 3 cut(s) 15, 99, 780
SfcI CTRYAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 575
SgrAI CRCCGGYG 1 cut(s) 743
SinI GGWCC 5 cut(s) 64, 158, 180, 652, 675
SmiMI CAYNNNNRTG 1 cut(s) 854
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 8 cut(s) 40, 154, 176, 329, 426, 593, 693, 759
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 3 cut(s) 128, 372, 387
TaqI TCGA 3 cut(s) 326, 372, 575
TaqII GACCGA 2 cut(s) 22, 175
TfiI GAWTC 3 cut(s) 577, 598, 1018
Tru1I TTAA 1 cut(s) 839
Tru9I TTAA 1 cut(s) 839
TseI GCWGC 3 cut(s) 433, 811, 967
TspDTI ATGAA 3 cut(s) 433, 898, 1001
TspGWI ACGGA 2 cut(s) 162, 789
VpaK11BI GGWCC 5 cut(s) 64, 158, 180, 652, 675
XmiI GTMKAC 3 cut(s) 103, 255, 326
ZraI GACGTC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.