Prupe.3G168400_v2.0.a1

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001741
Physical Location & Seq
Forward (+)
18720617 .. 18722772
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G168400.1

Sequence Viewer

Length: 1062 bp
ATGAATCGGATTCAACCACCGCTGGGAGCGAACCCGCCGCCCGCCAGGCGTGGGGACAACCCGAGACGACGTCGTCACGACCTAACCCTACCTCTCCCGCAGCGGGACGCGTCCCTCGCCGTGCCCTTGCCCCTTCCTCCGCCCGGACCCAGCTCGGCCCAATCGTCCTCGGCCCACAGCGCCGCCCAGCAGCTCATCAACTTCAACGAGCTCGAGCGCTCGAACCGCATCGGGAGCGGAACCGGAGGCACCGTCTACAAGGTCATCCACCGCCCGACGGGTCGTCTGTACGCGCTGAAAGTCATATACGGCAACCACGAGGAAGCCGTCCGCCGCCAGATCTGCCGCGAGATCGAAATCCTCCGCGGCGTCGACAACCCCAACGTTGTCAAGTGCCACGACATGTTCGACCACAACGGCGAGATCGAGGTGCTGCTCGAGTTAATGGACGGCGGGTCGCTGGAGGGCCAGCTCATCCAGAACGAGAAGACCCTCTCCGATTTGGCCAAGCAAATCTTGAGCGGCCTCGCCTACCTCCATAGACGTAAGATCGTGCATCGCGATATCAAGCCCTCGAATCTCTTGATCAACGCCAGGAACCAGGTCAAGATCGCCGATTTTGGGGTCAGTCGGATTTTGGAACAGACCATGGACCCTTGCAATTCTTCTGTGGGCACCATAGCTTATATGAGCCCTGAGAGGATCAATACTGATCTCAATCATGGCCAGTATGATGGGTATGCCGGTGATATATGGAGCCTTGGGGTCAGCATATTGGAATTCTACATGGGTCGGTTTCCGTTCTCTGTGGGAAGGTCAGGGGATTGGGCTAGCTTAATGTGGGCTATCTGTATGTCTCAGCCTCCTGAAGCTCCGCCCACTGCTTCGAGAGAGTTCAGGCATTTTATCTCTTGCTGTTTGCAGAGAGATCCTTCGAGGAGATTGTCGGCGGTGCAGTTGTTGCAGCATCCTTTTATCTCCGGCAATGGCGGCGGTCAGGCCCAGCAAGCACATCAGAATCTTCACCATCTTCTGCCACCACCCAGGCCTCTTCCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

354

Amino Acids

39.2

Weight (kDa)

9.08

Isoelectric Point (pI)

64.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 72
AatII GACGTC 1 cut(s) 73
AccB1I GGYRCC 2 cut(s) 248, 674
AccBSI CCGCTC 2 cut(s) 237, 522
AccI GTMKAC 2 cut(s) 255, 372
AccII CGCG 5 cut(s) 110, 293, 348, 366, 561
AclI AACGTT 1 cut(s) 384
AclWI GGATC 2 cut(s) 710, 923
AcoI YGGCCR 2 cut(s) 504, 724
AcsI RAATTY 1 cut(s) 779
AcuI CTGAAG 1 cut(s) 888
AcyI GRCGYC 2 cut(s) 70, 369
AfaI GTAC 1 cut(s) 290
AfeI AGCGCT 1 cut(s) 218
AfiI CCNNNNNNNGG 6 cut(s) 23, 51, 103, 143, 277, 621
AflIII ACRYGT 2 cut(s) 108, 402
AgsI TTSAA 2 cut(s) 14, 205
AhdI GACNNNNNGTC 2 cut(s) 282, 454
AjnI CCWGG 4 cut(s) 44, 593, 600, 1043
AluBI AGCT 7 cut(s) 153, 193, 211, 472, 683, 834, 872
AluI AGCT 7 cut(s) 153, 193, 211, 472, 683, 834, 872
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 58, 861
AlwI GGATC 2 cut(s) 710, 923
Ama87I CYCGRG 3 cut(s) 61, 212, 437
Aor51HI AGCGCT 1 cut(s) 218
AoxI GGCC 8 cut(s) 156, 171, 466, 504, 523, 724, 999, 1046
ApeKI GCWGC 4 cut(s) 100, 190, 433, 964
ApoI RAATTY 1 cut(s) 779
AspLEI GCGC 3 cut(s) 182, 219, 295
AspS9I GGNCC 6 cut(s) 146, 157, 172, 466, 652, 1000
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 758, 1016
AsuNHI GCTAGC 1 cut(s) 830
AvaI CYCGRG 3 cut(s) 61, 212, 437
AvaII GGWCC 2 cut(s) 146, 652
BaeGI GKGCMC 2 cut(s) 126, 677
BalI TGGCCA 2 cut(s) 506, 726
BanI GGYRCC 2 cut(s) 248, 674
BanII GRGCYC 2 cut(s) 213, 695
BauI CACGAG 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 112, 202, 420, 976
BccI CCATC 2 cut(s) 728, 1035
BceAI ACGGC 5 cut(s) 104, 311, 325, 433, 466
BciT130I CCWGG 4 cut(s) 46, 595, 602, 1045
BclI TGATCA 1 cut(s) 585
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 58, 861
BfaI CTAG 1 cut(s) 831
BfoI RGCGCY 2 cut(s) 183, 220
BglI GCCNNNNNGGC 1 cut(s) 46
BglII AGATCT 1 cut(s) 339
Bme1390I CCNGG 5 cut(s) 46, 144, 595, 602, 1045
Bme18I GGWCC 2 cut(s) 146, 652
BmeRI GACNNNNNGTC 2 cut(s) 282, 454
BmeT110I CYCGRG 3 cut(s) 61, 212, 437
BmgT120I GGNCC 6 cut(s) 146, 157, 172, 466, 652, 1000
BmiI GGNNCC 7 cut(s) 148, 241, 250, 599, 654, 676, 758
BmrFI CCNGG 5 cut(s) 46, 144, 595, 602, 1045
BmsI GCATC 3 cut(s) 237, 565, 976
BmtI GCTAGC 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 494
BpmI CTGGAG 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 538
BpuMI CCSGG 1 cut(s) 144
BsaBI GATNNNNATC 2 cut(s) 356, 717
BsaHI GRCGYC 2 cut(s) 70, 369
BsaJI CCNNGG 5 cut(s) 168, 364, 648, 760, 1043
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 51, 103, 143, 277, 621
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 1 cut(s) 991
Bse8I GATNNNNATC 2 cut(s) 356, 717
BseBI CCWGG 4 cut(s) 46, 595, 602, 1045
BseDI CCNNGG 5 cut(s) 168, 364, 648, 760, 1043
BseGI GGATG 3 cut(s) 264, 474, 967
BseJI GATNNNNATC 2 cut(s) 356, 717
BseLI CCNNNNNNNGG 6 cut(s) 23, 51, 103, 143, 277, 621
BseMI GCAATG 1 cut(s) 991
BseMII CTCAG 2 cut(s) 687, 872
BseNI ACTGG 1 cut(s) 727
BseRI GAGGAG 1 cut(s) 952
BseSI GKGCMC 2 cut(s) 126, 677
BseXI GCAGC 4 cut(s) 112, 202, 420, 976
BseYI CCCAGC 4 cut(s) 22, 149, 186, 1002
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 5 cut(s) 110, 293, 348, 366, 561
BshFI GGCC 8 cut(s) 158, 173, 468, 506, 525, 726, 1001, 1048
BshNI GGYRCC 2 cut(s) 248, 674
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 3 cut(s) 61, 212, 437
BsiSI CCGG 4 cut(s) 144, 243, 744, 981
BslFI GGGAC 3 cut(s) 68, 97, 119
BslI CCNNNNNNNGG 6 cut(s) 23, 51, 103, 143, 277, 621
BsmAI GTCTC 2 cut(s) 58, 861
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 3 cut(s) 68, 97, 119
BsnI GGCC 8 cut(s) 158, 173, 468, 506, 525, 726, 1001, 1048
BsoBI CYCGRG 3 cut(s) 61, 212, 437
Bsp1286I GDGCHC 4 cut(s) 126, 213, 677, 695
Bsp143I GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
Bsp19I CCATGG 1 cut(s) 648
Bsp68I TCGCGA 1 cut(s) 561
BspANI GGCC 8 cut(s) 158, 173, 468, 506, 525, 726, 1001, 1048
BspCNI CTCAG 2 cut(s) 688, 871
BspFNI CGCG 5 cut(s) 110, 293, 348, 366, 561
BspLI GGNNCC 7 cut(s) 148, 241, 250, 599, 654, 676, 758
BspOI GCTAGC 1 cut(s) 834
BspPI GGATC 2 cut(s) 710, 923
BspT107I GGYRCC 2 cut(s) 248, 674
BsrBI CCGCTC 2 cut(s) 237, 522
BsrDI GCAATG 1 cut(s) 991
BsrFI RCCGGY 1 cut(s) 743
BsrI ACTGG 1 cut(s) 727
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 5 cut(s) 168, 364, 648, 760, 1043
BssMI GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
BssNI GRCGYC 2 cut(s) 70, 369
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 2 cut(s) 648, 760
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 4 cut(s) 46, 595, 602, 1045
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 1056
BstACI GRCGYC 2 cut(s) 70, 369
BstAPI GCANNNNNTGC 1 cut(s) 961
BstC8I GCNNGC 4 cut(s) 42, 470, 832, 1008
BstDEI CTNAG 2 cut(s) 696, 858
BstDSI CCRYGG 2 cut(s) 364, 648
BstF5I GGATG 3 cut(s) 264, 474, 967
BstFNI CGCG 5 cut(s) 110, 293, 348, 366, 561
BstH2I RGCGCY 2 cut(s) 183, 220
BstHHI GCGC 3 cut(s) 182, 219, 295
BstKTI GATC 9 cut(s) 342, 354, 426, 552, 588, 612, 705, 715, 931
BstMAI GTCTC 2 cut(s) 58, 861
BstMBI GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
BstMWI GCNNNNNNNGC 9 cut(s) 46, 116, 179, 225, 234, 342, 961, 990, 1007
BstNI CCWGG 4 cut(s) 46, 595, 602, 1045
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 5 cut(s) 44, 142, 593, 600, 1043
BstSLI GKGCMC 2 cut(s) 126, 677
BstUI CGCG 5 cut(s) 110, 293, 348, 366, 561
BstV1I GCAGC 4 cut(s) 112, 202, 420, 976
BstV2I GAAGAC 1 cut(s) 494
BstX2I RGATCY 2 cut(s) 339, 928
BstXI CCANNNNNNTGG 1 cut(s) 734
BstYI RGATCY 2 cut(s) 339, 928
BsuRI GGCC 8 cut(s) 158, 173, 468, 506, 525, 726, 1001, 1048
BtgI CCRYGG 2 cut(s) 364, 648
BtgZI GCGATG 1 cut(s) 542
BtsCI GGATG 3 cut(s) 264, 474, 967
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 1 cut(s) 879
BtuMI TCGCGA 1 cut(s) 561
Cac8I GCNNGC 4 cut(s) 42, 470, 832, 1008
CfoI GCGC 3 cut(s) 182, 219, 295
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 6 cut(s) 146, 157, 172, 466, 652, 1000
Cfr42I CCGCGG 1 cut(s) 367
CseI GACGC 3 cut(s) 99, 116, 358
CsiI ACCWGGT 1 cut(s) 600
Csp6I GTAC 1 cut(s) 289
CviAII CATG 4 cut(s) 403, 649, 722, 787
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 2 cut(s) 696, 858
DpnI GATC 9 cut(s) 341, 353, 425, 551, 587, 611, 704, 714, 930
DpnII GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
DrdI GACNNNNNNGTC 1 cut(s) 72
DriI GACNNNNNGTC 2 cut(s) 282, 454
DseDI GACNNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 504, 724
Eam1104I CTCTTC 1 cut(s) 1056
Eam1105I GACNNNNNGTC 2 cut(s) 282, 454
EarI CTCTTC 1 cut(s) 1056
EciI GGCGGA 3 cut(s) 129, 320, 864
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 648, 760
Eco147I AGGCCT 1 cut(s) 1048
Eco24I GRGCYC 2 cut(s) 213, 695
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 2 cut(s) 146, 652
Eco47III AGCGCT 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 888
Eco88I CYCGRG 3 cut(s) 61, 212, 437
EcoICRI GAGCTC 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 779
EcoRII CCWGG 4 cut(s) 44, 593, 600, 1043
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 2 cut(s) 648, 760
EcoT38I GRGCYC 2 cut(s) 213, 695
ErhI CCWWGG 2 cut(s) 648, 760
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 4 cut(s) 406, 652, 725, 790
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 3 cut(s) 68, 97, 119
FatI CATG 4 cut(s) 402, 648, 721, 786
FauI CCCGC 5 cut(s) 42, 49, 96, 105, 446
FbaI TGATCA 1 cut(s) 585
FblI GTMKAC 2 cut(s) 255, 372
FokI GGATG 3 cut(s) 251, 461, 954
FriOI GRGCYC 2 cut(s) 213, 695
FspBI CTAG 1 cut(s) 831
GlaI GCGC 3 cut(s) 181, 218, 294
GsaI CCCAGC 4 cut(s) 26, 153, 190, 1006
GsuI CTGGAG 1 cut(s) 482
HaeII RGCGCY 2 cut(s) 183, 220
HaeIII GGCC 8 cut(s) 158, 173, 468, 506, 525, 726, 1001, 1048
HapII CCGG 4 cut(s) 144, 243, 744, 981
HgaI GACGC 3 cut(s) 99, 116, 358
HhaI GCGC 3 cut(s) 182, 219, 295
Hin1I GRCGYC 2 cut(s) 70, 369
Hin1II CATG 4 cut(s) 406, 652, 725, 790
Hin6I GCGC 3 cut(s) 180, 217, 293
HinP1I GCGC 3 cut(s) 180, 217, 293
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 4 cut(s) 4, 10, 577, 1018
HpaII CCGG 4 cut(s) 144, 243, 744, 981
HphI GGTGA 2 cut(s) 758, 1016
Hpy166II GTNNAC 2 cut(s) 256, 373
Hpy188I TCNGA 4 cut(s) 9, 499, 633, 1017
Hpy188III TCNNGA 9 cut(s) 77, 232, 478, 517, 560, 583, 607, 866, 888
Hpy8I GTNNAC 2 cut(s) 256, 373
Hpy99I CGWCG 4 cut(s) 72, 75, 280, 374
HpyAV CCTTC 3 cut(s) 143, 807, 942
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 3 cut(s) 70, 384, 544
HpyCH4V TGCA 5 cut(s) 556, 660, 922, 955, 964
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 116, 179, 225, 234, 342, 961, 990, 1007
HpyF3I CTNAG 2 cut(s) 696, 858
HpySE526I ACGT 3 cut(s) 70, 384, 544
Hsp92I GRCGYC 2 cut(s) 70, 369
Hsp92II CATG 4 cut(s) 406, 652, 725, 790
HspAI GCGC 3 cut(s) 180, 217, 293
Ksp22I TGATCA 1 cut(s) 585
KspI CCGCGG 1 cut(s) 367
Kzo9I GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
LmnI GCTCC 4 cut(s) 26, 234, 756, 877
Lsp1109I GCAGC 4 cut(s) 112, 202, 420, 976
LweI GCATC 3 cut(s) 237, 565, 976
MabI ACCWGGT 1 cut(s) 600
MaeI CTAG 1 cut(s) 831
MaeII ACGT 3 cut(s) 70, 384, 544
MaeIII GTNAC 1 cut(s) 74
MalI GATC 9 cut(s) 341, 353, 425, 551, 587, 611, 704, 714, 930
MbiI CCGCTC 2 cut(s) 237, 522
MboI GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
MboII GAAGA 5 cut(s) 499, 657, 1013, 1022, 1043
MflI RGATCY 2 cut(s) 339, 928
MhlI GDGCHC 4 cut(s) 126, 213, 677, 695
MlsI TGGCCA 2 cut(s) 506, 726
MluCI AATT 2 cut(s) 661, 779
MluI ACGCGT 1 cut(s) 108
MluNI TGGCCA 2 cut(s) 506, 726
MmeI TCCRAC 1 cut(s) 611
Mox20I TGGCCA 2 cut(s) 506, 726
MscI TGGCCA 2 cut(s) 506, 726
MseI TTAA 3 cut(s) 443, 836, 1060
Msp20I TGGCCA 2 cut(s) 506, 726
MspA1I CMGCKG 3 cut(s) 22, 103, 366
MspI CCGG 4 cut(s) 144, 243, 744, 981
MspR9I CCNGG 5 cut(s) 46, 144, 595, 602, 1045
MvaI CCWGG 4 cut(s) 46, 595, 602, 1045
MvnI CGCG 5 cut(s) 110, 293, 348, 366, 561
MwoI GCNNNNNNNGC 9 cut(s) 46, 116, 179, 225, 234, 342, 961, 990, 1007
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 648
NdeII GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
NheI GCTAGC 1 cut(s) 830
NlaIII CATG 4 cut(s) 406, 652, 725, 790
NlaIV GGNNCC 7 cut(s) 148, 241, 250, 599, 654, 676, 758
NmeAIII GCCGAG 2 cut(s) 134, 149
NmuCI GTSAC 1 cut(s) 74
NruI TCGCGA 1 cut(s) 561
NspI RCATGY 1 cut(s) 406
PaeR7I CTCGAG 2 cut(s) 212, 437
PceI AGGCCT 1 cut(s) 1048
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 6 cut(s) 161, 315, 324, 405, 423, 558
PfeI GAWTC 4 cut(s) 4, 10, 577, 1018
PflFI GACNNNGTC 2 cut(s) 69, 72
PscI ACATGT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 213
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 4 cut(s) 44, 593, 600, 1043
PspFI CCCAGC 4 cut(s) 22, 149, 186, 1002
PspGI CCWGG 4 cut(s) 44, 593, 600, 1043
PspN4I GGNNCC 7 cut(s) 148, 241, 250, 599, 654, 676, 758
PspPI GGNCC 6 cut(s) 146, 157, 172, 466, 652, 1000
PspXI VCTCGAGB 2 cut(s) 212, 437
PsuI RGATCY 2 cut(s) 339, 928
PsyI GACNNNGTC 2 cut(s) 69, 72
RruI TCGCGA 1 cut(s) 561
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 213
SacII CCGCGG 1 cut(s) 367
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 3 cut(s) 443, 836, 1060
Sau3AI GATC 9 cut(s) 339, 351, 423, 549, 585, 609, 702, 712, 928
Sau96I GGNCC 6 cut(s) 146, 157, 172, 466, 652, 1000
ScrFI CCNGG 5 cut(s) 46, 144, 595, 602, 1045
SduI GDGCHC 4 cut(s) 126, 213, 677, 695
SexAI ACCWGGT 1 cut(s) 600
SfaNI GCATC 3 cut(s) 237, 565, 976
Sfr274I CTCGAG 2 cut(s) 212, 437
Sfr303I CCGCGG 1 cut(s) 367
SgrBI CCGCGG 1 cut(s) 367
SinI GGWCC 2 cut(s) 146, 652
SlaI CTCGAG 2 cut(s) 212, 437
SmlI CTYRAG 3 cut(s) 212, 437, 517
SmoI CTYRAG 3 cut(s) 212, 437, 517
Sse9I AATT 2 cut(s) 661, 779
SseBI AGGCCT 1 cut(s) 1048
SspMI CTAG 1 cut(s) 831
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 1048
StyD4I CCNGG 5 cut(s) 44, 142, 593, 600, 1043
StyI CCWWGG 2 cut(s) 648, 760
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 3 cut(s) 73, 387, 547
TasI AATT 2 cut(s) 661, 779
TauI GCSGC 7 cut(s) 40, 185, 336, 348, 369, 525, 993
TfiI GAWTC 4 cut(s) 4, 10, 577, 1018
Tru1I TTAA 3 cut(s) 443, 836, 1060
Tru9I TTAA 3 cut(s) 443, 836, 1060
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 4 cut(s) 100, 190, 433, 964
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 1 cut(s) 886
Tth111I GACNNNGTC 2 cut(s) 69, 72
VpaK11BI GGWCC 2 cut(s) 146, 652
XapI RAATTY 1 cut(s) 779
XceI RCATGY 1 cut(s) 406
XhoI CTCGAG 2 cut(s) 212, 437
XmiI GTMKAC 2 cut(s) 255, 372
XspI CTAG 1 cut(s) 831
ZraI GACGTC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.