Rorug06G0347200

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001741
Physical Location & Seq
Forward (+)
49783286 .. 49785207
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0347200.1

Sequence Viewer

Length: 648 bp
ATGGCCGGCATCAAAGTCCATGGAACCCCTTTCTCAACGGCAGCAGCTCGAGTTTTGGCTACCCTCTATGAGAAAGAGGTTGAATTCGAGTTTGTTCCGATAGACATGAGAGCTGGTGAACATAAAAAGGAGTCCTTCCTTGCCCTCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGACGGGGATCTTCAGCTTTTTGAGTCAAGAGCAATTACACAATACATTGCCCATGAGTATGCTCCAAAGGGAACCCCGCTGATTTTCCAAGACTCAAAGAAGATGGCAATTCTATCGGTGTGGACAGAGGTGGAGGCTCAAAAATACGACCCAGTAGCTTCAAAACTGACATTTGAGCTAGTTATAAAACCTCTGGTACTTGGCTTGGCCACAGACTTCACAGTCGTGGAGGAATTTGAAGCTAAGTTGGGTACAGTTCTCGATGTCTATGAGACTCGTCTGGGCAAATCGAAATACTTGGGAGGTGATTGCTTCAGCTTGGCAGATCTTCACCACCTTCCAACCACAGACTACTTGATGGGAACACAAGCCAAGAAGCTGTTCGAGTGCCGCCCAAATGTTAGCGCATGGGTAGCTGATATCACAGCAAGGCCTGCTTGGAAAAAAGTCGTTGCCATGAGAGCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

215

Amino Acids

23.99

Weight (kDa)

5.89

Isoelectric Point (pI)

25.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 4 - 76 4.1e-21 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 81 1.4e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 11 - 77 2e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 115 - 204 2.4e-13 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 138 - 208 4e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AasI GACNNNNNNGTC 1 cut(s) 398
AciI CCGC 2 cut(s) 254, 568
AclWI GGATC 1 cut(s) 192
AcoI YGGCCR 2 cut(s) 3, 384
AcsI RAATTY 2 cut(s) 83, 410
AcuI CTGAAG 2 cut(s) 173, 475
AfaI GTAC 2 cut(s) 375, 430
AgsI TTSAA 4 cut(s) 83, 176, 339, 416
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AleI CACNNNNGTG 1 cut(s) 401
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 192
Ama87I CYCGRG 1 cut(s) 48
AoxI GGCC 3 cut(s) 3, 384, 608
ApeKI GCWGC 2 cut(s) 41, 44
ApoI RAATTY 2 cut(s) 83, 410
Asp700I GAANNNNTTC 1 cut(s) 557
AspLEI GCGC 1 cut(s) 584
AsuHPI GGTGA 3 cut(s) 128, 494, 500
AvaI CYCGRG 1 cut(s) 48
BalI TGGCCA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 2 cut(s) 53, 56
BccI CCATC 2 cut(s) 274, 529
BceAI ACGGC 1 cut(s) 54
BcgI CGANNNNNNTGC 2 cut(s) 273, 307
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 1 cut(s) 356
BglII AGATCT 1 cut(s) 502
BisI GCNGC 3 cut(s) 42, 45, 568
BlsI GCNGC 3 cut(s) 43, 46, 569
BmeT110I CYCGRG 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 25, 250
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 19
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 19
BseMI GCAATG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 53, 56
BshFI GGCC 3 cut(s) 5, 386, 610
BsiHKAI GWGCWC 1 cut(s) 643
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 1 cut(s) 6
BsmAI GTCTC 1 cut(s) 443
BsnI GGCC 3 cut(s) 5, 386, 610
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 2 cut(s) 184, 502
Bsp19I CCATGG 1 cut(s) 19
BspACI CCGC 2 cut(s) 254, 568
BspANI GGCC 3 cut(s) 5, 386, 610
BspLI GGNNCC 2 cut(s) 25, 250
BspPI GGATC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 2 cut(s) 184, 502
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 2 cut(s) 400, 433
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 2 cut(s) 7, 612
BstDEI CTNAG 1 cut(s) 420
BstDSI CCRYGG 1 cut(s) 19
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 2 cut(s) 187, 505
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 184, 502
BstMWI GCNNNNNNNGC 3 cut(s) 590, 611, 638
BstV1I GCAGC 2 cut(s) 53, 56
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 2 cut(s) 184, 502
BstYI RGATCY 2 cut(s) 184, 502
BsuRI GGCC 3 cut(s) 5, 386, 610
BtgI CCRYGG 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 7, 612
CfoI GCGC 1 cut(s) 584
Cfr10I RCCGGY 1 cut(s) 5
Csp6I GTAC 2 cut(s) 374, 429
CviAII CATG 5 cut(s) 20, 106, 230, 585, 634
CviQI GTAC 2 cut(s) 374, 429
DdeI CTNAG 1 cut(s) 420
DpnI GATC 2 cut(s) 186, 504
DpnII GATC 2 cut(s) 184, 502
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 2 cut(s) 3, 384
Ecl136II GAGCTC 1 cut(s) 641
Eco130I CCWWGG 1 cut(s) 19
Eco147I AGGCCT 1 cut(s) 610
Eco24I GRGCYC 1 cut(s) 643
Eco32I GATATC 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 641
Eco57I CTGAAG 2 cut(s) 173, 475
Eco88I CYCGRG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 83
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 5 cut(s) 23, 109, 233, 588, 637
FalI AAGNNNNNCTT 4 cut(s) 119, 151, 598, 630
FatI CATG 5 cut(s) 19, 105, 229, 584, 633
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 3 cut(s) 42, 45, 568
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 3 cut(s) 42, 45, 568
FspBI CTAG 1 cut(s) 356
GlaI GCGC 1 cut(s) 583
GluI GCNGC 3 cut(s) 42, 45, 568
HaeIII GGCC 3 cut(s) 5, 386, 610
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 584
Hin1II CATG 5 cut(s) 23, 109, 233, 588, 637
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HinfI GANTC 4 cut(s) 131, 200, 269, 451
HpaII CCGG 1 cut(s) 6
HphI GGTGA 3 cut(s) 128, 494, 500
Hpy166II GTNNAC 2 cut(s) 119, 300
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 2 cut(s) 204, 437
Hpy8I GTNNAC 2 cut(s) 119, 300
HpyAV CCTTC 2 cut(s) 145, 524
HpyCH4III ACNGT 2 cut(s) 400, 433
HpyF10VI GCNNNNNNNGC 3 cut(s) 590, 611, 638
HpyF3I CTNAG 1 cut(s) 420
Hsp92II CATG 5 cut(s) 23, 109, 233, 588, 637
HspAI GCGC 1 cut(s) 582
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 2 cut(s) 184, 502
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 7 cut(s) 19, 99, 180, 342, 356, 443, 624
Lsp1109I GCAGC 2 cut(s) 53, 56
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 356
MalI GATC 2 cut(s) 186, 504
MboI GATC 2 cut(s) 184, 502
MboII GAAGA 4 cut(s) 179, 188, 289, 497
MflI RGATCY 2 cut(s) 184, 502
MhlI GDGCHC 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 4 cut(s) 83, 210, 285, 410
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 4 cut(s) 140, 209, 263, 445
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 8 cut(s) 70, 74, 155, 298, 304, 378, 400, 473
Mox20I TGGCCA 1 cut(s) 386
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 557
MscI TGGCCA 1 cut(s) 386
MslI CAYNNNNRTG 2 cut(s) 234, 401
Msp20I TGGCCA 1 cut(s) 386
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 1 cut(s) 6
MwoI GCNNNNNNNGC 3 cut(s) 590, 611, 638
NaeI GCCGGC 1 cut(s) 7
NcoI CCATGG 1 cut(s) 19
NdeII GATC 2 cut(s) 184, 502
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 23, 109, 233, 588, 637
NlaIV GGNNCC 2 cut(s) 25, 250
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 48
PceI AGGCCT 1 cut(s) 610
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 557
PkrI GCNGC 3 cut(s) 43, 46, 569
PleI GAGTC 4 cut(s) 139, 208, 263, 445
PpsI GAGTC 4 cut(s) 139, 208, 263, 445
PsiI TTATAA 1 cut(s) 362
Psp124BI GAGCTC 1 cut(s) 643
PspN4I GGNNCC 2 cut(s) 25, 250
PspXI VCTCGAGB 1 cut(s) 48
PsuI RGATCY 2 cut(s) 184, 502
RsaI GTAC 2 cut(s) 375, 430
RsaNI GTAC 2 cut(s) 374, 429
RseI CAYNNNNRTG 2 cut(s) 234, 401
SacI GAGCTC 1 cut(s) 643
SatI GCNGC 3 cut(s) 42, 45, 568
Sau3AI GATC 2 cut(s) 184, 502
SchI GAGTC 4 cut(s) 140, 209, 263, 445
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 1 cut(s) 18
Sfr274I CTCGAG 1 cut(s) 48
SlaI CTCGAG 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 234, 401
SmlI CTYRAG 1 cut(s) 48
SmoI CTYRAG 1 cut(s) 48
Sse9I AATT 4 cut(s) 83, 210, 285, 410
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 2 cut(s) 254, 568
SspMI CTAG 1 cut(s) 356
SstI GAGCTC 1 cut(s) 643
StuI AGGCCT 1 cut(s) 610
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 2 cut(s) 400, 433
TaqI TCGA 5 cut(s) 49, 87, 438, 467, 561
TasI AATT 4 cut(s) 83, 210, 285, 410
TauI GCSGC 1 cut(s) 570
TseI GCWGC 2 cut(s) 41, 44
XapI RAATTY 2 cut(s) 83, 410
XhoI CTCGAG 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 557
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.