Rroxscaffold_2G00100130

TIFY 10B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013700
Physical Location & Seq
Reverse (-)
21991881 .. 21992527
647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100130.1

Sequence Viewer

Length: 429 bp
ATGGCCGAGAGATCCAACTTTGCGCAGACCTGCAACCTCCTCAGCCAGTACTTGAAGGAGAAGAGAAGCCAGTACCTCCAGGGAGATAGCTTCGGAGTGAAGCCAGCCCCGGCGACCATGAACTTGCTCAACACCATGGAGGCCGGCCCTCCAGCGGCGGTGAGTCCAGTGGGCCAGGCTCCGGATCAGCCCAGATCTGCTCCTATGACCATCTTTTACGGCGGGCAGGTCCTGGTTTTCAACGACGTTTCGGCTGAGAAGGCAAAGGAAATTATGGGATTTGGCTACCAAAGGAAGCGCTGTGTTCTTATTGGGTTGTTTGAATTTCAGAGTGACTGCAGTAGCTCCATACCAACTGAACCACATCCAGAGAGCAGCTTCTCCCAAGGCAGAGGAACGAGTAGGAGTGCAAAGTTCAAAGCAGCTTGA

Protein Analysis

142

Amino Acids

15.42

Weight (kDa)

8.84

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tify PF06200 63 - 92 4.5e-16 tify domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 24
Acc36I ACCTGC 2 cut(s) 38, 217
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 3 cut(s) 155, 158, 222
AclWI GGATC 2 cut(s) 6, 192
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 2 cut(s) 50, 74
AfeI AGCGCT 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 154, 181
AgsI TTSAA 4 cut(s) 55, 241, 323, 418
AjnI CCWGG 3 cut(s) 78, 174, 231
AluBI AGCT 4 cut(s) 90, 345, 378, 425
AluI AGCT 4 cut(s) 90, 345, 378, 425
AlwI GGATC 2 cut(s) 6, 192
AlwNI CAGNNNCTG 1 cut(s) 232
Aor13HI TCCGGA 1 cut(s) 181
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 4 cut(s) 3, 141, 145, 172
ApeKI GCWGC 2 cut(s) 375, 422
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 2 cut(s) 25, 300
AspS9I GGNCC 3 cut(s) 146, 172, 229
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 172
AvaII GGWCC 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 387
BccI CCATC 1 cut(s) 218
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 3 cut(s) 80, 176, 233
BcnI CCSGG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 1 cut(s) 301
BfuAI ACCTGC 2 cut(s) 38, 217
BglII AGATCT 1 cut(s) 194
BisI GCNGC 3 cut(s) 156, 376, 423
BlsI GCNGC 3 cut(s) 157, 377, 424
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 80, 110, 176, 233
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 146, 172, 229
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 4 cut(s) 80, 110, 176, 233
BpmI CTGGAG 2 cut(s) 62, 135
Bpu10I CCTNAGC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 110
BsaJI CCNNGG 4 cut(s) 79, 108, 135, 385
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 181
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 46, 70, 167
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 3 cut(s) 80, 176, 233
BseDI CCNNGG 4 cut(s) 79, 108, 135, 385
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 2 cut(s) 154, 181
BseMII CTCAG 2 cut(s) 55, 246
BseNI ACTGG 3 cut(s) 46, 70, 167
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 1 cut(s) 387
BshFI GGCC 4 cut(s) 5, 143, 147, 174
BsiSI CCGG 3 cut(s) 110, 144, 182
BslI CCNNNNNNNGG 2 cut(s) 154, 181
BsnI GGCC 4 cut(s) 5, 143, 147, 174
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 3 cut(s) 11, 184, 194
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 3 cut(s) 155, 158, 222
BspANI GGCC 4 cut(s) 5, 143, 147, 174
BspCNI CTCAG 2 cut(s) 54, 247
BspEI TCCGGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 341
BspMI ACCTGC 2 cut(s) 38, 217
BspPI GGATC 2 cut(s) 6, 192
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 3 cut(s) 46, 70, 167
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 4 cut(s) 79, 108, 135, 385
BssMI GATC 3 cut(s) 11, 184, 194
BssT1I CCWWGG 2 cut(s) 135, 385
Bst2UI CCWGG 3 cut(s) 80, 176, 233
Bst6I CTCTTC 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 105, 145, 224
BstDEI CTNAG 2 cut(s) 41, 255
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 364
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 2 cut(s) 25, 300
BstKTI GATC 3 cut(s) 14, 187, 197
BstMBI GATC 3 cut(s) 11, 184, 194
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 3 cut(s) 80, 176, 233
BstSCI CCNGG 4 cut(s) 78, 108, 174, 231
BstSFI CTRYAG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 11, 194
BstYI RGATCY 2 cut(s) 11, 194
BsuRI GGCC 4 cut(s) 5, 143, 147, 174
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 174
BveI ACCTGC 2 cut(s) 38, 217
Cac8I GCNNGC 3 cut(s) 105, 145, 224
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 2 cut(s) 25, 300
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 3 cut(s) 146, 172, 229
Csp6I GTAC 2 cut(s) 49, 73
CviAII CATG 2 cut(s) 118, 136
CviQI GTAC 2 cut(s) 49, 73
DdeI CTNAG 2 cut(s) 41, 255
DpnI GATC 3 cut(s) 13, 186, 196
DpnII GATC 3 cut(s) 11, 184, 194
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 135, 385
Eco47I GGWCC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 3 cut(s) 78, 174, 231
EcoT14I CCWWGG 2 cut(s) 135, 385
ErhI CCWWGG 2 cut(s) 135, 385
FaeI CATG 2 cut(s) 121, 139
FaiI YATR 5 cut(s) 119, 137, 206, 275, 350
FatI CATG 2 cut(s) 117, 135
FauI CCCGC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 156, 376, 423
FokI GGATG 1 cut(s) 351
FseI GGCCGGCC 1 cut(s) 147
Fsp4HI GCNGC 3 cut(s) 156, 376, 423
FspI TGCGCA 1 cut(s) 24
GlaI GCGC 2 cut(s) 24, 299
GluI GCNGC 3 cut(s) 156, 376, 423
GsuI CTGGAG 2 cut(s) 62, 135
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 4 cut(s) 5, 143, 147, 174
HapII CCGG 3 cut(s) 110, 144, 182
HhaI GCGC 2 cut(s) 25, 300
Hin1II CATG 2 cut(s) 121, 139
Hin6I GCGC 2 cut(s) 23, 298
HinP1I GCGC 2 cut(s) 23, 298
HinfI GANTC 1 cut(s) 163
HpaII CCGG 3 cut(s) 110, 144, 182
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 95, 330
Hpy188III TCNNGA 2 cut(s) 182, 368
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 49, 253
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 3 cut(s) 33, 339, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 41, 255
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 2 cut(s) 121, 139
HspAI GCGC 2 cut(s) 23, 298
Kpn2I TCCGGA 1 cut(s) 181
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 3 cut(s) 11, 184, 194
LmnI GCTCC 3 cut(s) 184, 205, 350
Lsp1109I GCAGC 1 cut(s) 387
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 332
MalI GATC 3 cut(s) 13, 186, 196
MboI GATC 3 cut(s) 11, 184, 194
MboII GAAGA 1 cut(s) 73
MflI RGATCY 2 cut(s) 11, 194
MluCI AATT 2 cut(s) 270, 323
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 6 cut(s) 47, 50, 86, 133, 159, 386
MroI TCCGGA 1 cut(s) 181
MroNI GCCGGC 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 110, 144, 182
MspR9I CCNGG 4 cut(s) 80, 110, 176, 233
MvaI CCWGG 3 cut(s) 80, 176, 233
MwoI GCNNNNNNNGC 1 cut(s) 260
NaeI GCCGGC 1 cut(s) 145
NciI CCSGG 1 cut(s) 110
NcoI CCATGG 1 cut(s) 135
NdeII GATC 3 cut(s) 11, 184, 194
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 2 cut(s) 121, 139
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 332
NsbI TGCGCA 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 145
PkrI GCNGC 3 cut(s) 157, 377, 424
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 229
Psp6I CCWGG 3 cut(s) 78, 174, 231
PspGI CCWGG 3 cut(s) 78, 174, 231
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 3 cut(s) 146, 172, 229
PspPPI RGGWCCY 1 cut(s) 229
PstI CTGCAG 1 cut(s) 341
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 11, 194
RigI GGCCGGCC 1 cut(s) 147
RsaI GTAC 2 cut(s) 50, 74
RsaNI GTAC 2 cut(s) 49, 73
SatI GCNGC 3 cut(s) 156, 376, 423
Sau3AI GATC 3 cut(s) 11, 184, 194
Sau96I GGNCC 3 cut(s) 146, 172, 229
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 4 cut(s) 80, 110, 176, 233
SetI ASST 9 cut(s) 32, 39, 78, 92, 231, 249, 347, 380, 427
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 229
Sse9I AATT 2 cut(s) 270, 323
SsiI CCGC 3 cut(s) 155, 158, 222
StyD4I CCNGG 4 cut(s) 78, 108, 174, 231
StyI CCWWGG 2 cut(s) 135, 385
TaiI ACGT 1 cut(s) 249
TasI AATT 2 cut(s) 270, 323
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 158
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 375, 422
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 134
TspRI CASTG 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 1 cut(s) 323
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.